LOCUS       RC223382                7824 bp    DNA     circular  SYN 
DEFINITION  ANO3 (Myc-DDK-tagged)-Human anoctamin 3 (ANO3)
ACCESSION   NM_031418
VERSION     NM_031418.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3981..4072 
                     /label="Myc-DDK"  
     misc_feature    4102..4687 
                     /label="PolyA signal"  
     misc_feature    4159..4178 
                     /label="Restriction Enzyme"  
     misc_feature    4963..5635 
                     /label="ColE1"  
     misc_feature    6065..6859 
                     /label="Kan/Neo"  
     misc_feature    6894..7196 
                     /label="SV40 ori"  
     misc_feature    7282..7759 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3963..3969 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3963 
                     /label="ANO3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCACCAT TCAGGCTCCA TTCAGTCCTT TAAACAGCAA AAAGGTATGA 
     1081 ATATAAGCAA GAGTGAGATA ACAAAAGAAA CTTCGTTAAA ACCGTCTCGG AGATCCCTGC 
     1141 CTTGCCTCGC CCAGAGCTAC GCTTACTCAA AGAGCTTGAG CCAGTCTACT TCCCTCTTCC 
     1201 AGTCAACCGA GAGTGAATCT CAGGCTCCCA CATCTATAAC CTTAATCTCC ACTGACAAAG 
     1261 CAGAGCAAGT TAATACTGAG GAGAATAAAA ACGACTCTGT GCTGAGATGT TCATTTGCTG 
     1321 ACCTCAGCGA TTTTTGTTTG GCCCTAGGAA AAGATAAGGA TTACACGGAT GAATCAGAAC 
     1381 ACGCTACTTA TGACCGATCT CGTCTCATTA ATGACTTTGT TATCAAAGAT AAATCTGAAT 
     1441 TCAAGACAAA ATTATCTAAG AATGACATGA ATTACATAGC ATCCAGTGGA CCTCTGTTCA 
     1501 AAGATGGCAA AAAGAGAATT GATTACATCT TGGTTTATAG AAAGACAAAT ATACAATATG 
     1561 ATAAAAGAAA CACATTTGAA AAGAACCTCA GAGCAGAAGG CTTGATGTTG GAGAAGGAGC 
     1621 CAGCTATTGC AAGCCCCGAT ATCATGTTTA TTAAAATTCA CATTCCATGG GACACGCTGT 
     1681 GCAAGTATGC AGAGAGGCTG AATATCAGGA TGCCCTTCAG GAAAAAATGC TATTACACTG 
     1741 ACGGGAGGAG CAAATCAATG GGCAGGATGC AAACTTATTT TAGAAGAATC AAAAACTGGA 
     1801 TGGCCCAAAA CCCAATGGTT CTTGACAAGT CAGCTTTTCC AGACCTAGAG GAGTCAGACT 
     1861 GCTATACTGG CCCCTTCAGC CGTGCACGGA TTCACCACTT CATAATAAAT AATAAAGACA 
     1921 CCTTCTTCAG CAATGCTACT CGAAGCAGAA TAGTCTATCA CATGCTGGAA CGCACCAAAT 
     1981 ATGAAAATGG AATATCAAAA GTGGGTATCC GTAAACTTAT AAACAATGGC TCATACATAG 
     2041 CAGCGTTTCC ACCACATGAG GGAGCCTACA AAAGTAGCCA GCCCATTAAA ACCCATGGAC 
     2101 CTCAGAATAA CAGACATCTA TTATATGAGC GCTGGGCACG CTGGGGAATG TGGTATAAGC 
     2161 ATCAGCCTCT GGATTTAATC AGGCTATACT TTGGTGAGAA GATTGGACTA TACTTTGCTT 
     2221 GGCTGGGATG GTATACTGGA ATGTTGATTC CTGCAGCAAT TGTTGGTTTG TGCGTTTTCT 
     2281 TCTATGGATT ATTTACAATG AATAATAGTC AAGTAAGCCA AGAAATTTGT AAAGCCACTG 
     2341 AAGTCTTTAT GTGCCCTCTC TGTGACAAGA ACTGCTCCCT GCAGAGACTC AACGACAGCT 
     2401 GTATCTATGC CAAGGTGACA TATTTGTTCG ATAATGGAGG GACAGTCTTC TTTGCTATTT 
     2461 TTATGGCAAT ATGGGCCACA GTCTTCCTGG AGTTTTGGAA AAGGAGAAGG AGTATACTGA 
     2521 CCTATACTTG GGACCTTATC GAATGGGAAG AAGAGGAGGA AACACTTCGT CCCCAGTTTG 
     2581 AAGCCAAGTA TTACAAGATG GAGATTGTAA ATCCCATCAC GGGAAAACCT GAACCACATC 
     2641 AGCCTTCCTC AGACAAAGTC ACTCGTCTTC TTGTTTCTGT CTCAGGAATA TTCTTCATGA 
     2701 TATCCTTGGT GATCACTGCA GTGTTTGGAG TTGTGGTGTA CCGCCTGGTT GTCATGGAAC 
     2761 AGTTTGCATC ATTCAAGTGG AATTTCATCA AACAATACTG GCAGTTTGCA ACATCTGCTG 
     2821 CTGCTGTCTG TATCAATTTC ATAATCATTA TGTTGCTGAA TCTTGCTTAT GAAAAAATTG 
     2881 CTTACCTCCT CACCAATTTA GAATATCCTC GAACAGAATC AGAGTGGGAA AACAGCTTCG 
     2941 CCCTGAAGAT GTTCCTCTTC CAGTTTGTCA ATTTAAACAG TTCCATCTTC TATATCGCTT 
     3001 TCTTTTTGGG AAGATTCGTA GGCCACCCAG GAAAATACAA TAAACTTTTT GACCGGTGGA 
     3061 GACTGGAGGA ATGTCATCCT AGTGGCTGTT TGATAGACCT CTGCCTCCAG ATGGGTGTCA 
     3121 TCATGTTTTT GAAGCAAATA TGGAACAACT TCATGGAACT AGGATACCCG TTGATCCAGA 
     3181 ACTGGTGGTC ACGACATAAA ATCAAGCGGG GAATACATGA TGCTTCCATA CCTCAGTGGG 
     3241 AAAATGATTG GAATCTGCAG CCCATGAACC TTCATGGACT GATGGATGAG TACTTAGAAA 
     3301 TGGTTTTGCA ATTTGGTTTT ACCACCATCT TTGTTGCGGC TTTTCCTCTA GCCCCTCTTT 
     3361 TGGCTTTGTT AAACAATATC ATTGAAATCA GGCTGGATGC ATACAAATTT GTCACTCAAT 
     3421 GGCGGAGGCC TTTGCCAGCC CGAGCAACTG ACATAGGTAT CTGGCTTGGA ATTCTCGAAG 
     3481 GAATCGGTAT ATTGGCTGTG ATCACCAATG CATTTGTAAT TGCTATTACT TCTGATTACA 
     3541 TCCCACGTTT TGTTTATGAA TACAAATATG GCCCCTGTGC AAATCATGTA GAACCAAGTG 
     3601 AAAATTGCTT GAAGGGATAT GTCAACAATA GCCTATCCTT CTTTGACCTG AGTGAGCTTG 
     3661 GTATGGGAAA ATCTGGTTAT TGCAGGTACA GAGACTACAG AGGCCCGCCC TGGAGTTCCA 
     3721 AACCCTATGA GTTTACTTTA CAATACTGGC ATATCCTTGC TGCTAGATTG GCCTTCATTA 
     3781 TTGTGTTTGA GCACCTTGTT TTTGGGATTA AGTCATTCAT CGCATACCTG ATTCCAGACG 
     3841 TACCAAAGGG TCTACATGAC CGAATACGAC GAGAGAAGTA CTTAGTTCAA GAAATGATGT 
     3901 ATGAGGCTGA ACTGGAACAT TTGCAACAAC AACGGAGAAA AAGTGGTCAG CCTGTTCACC 
     3961 ATGAATGGCC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4021 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4081 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4141 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4201 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4261 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4321 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4381 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4441 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4501 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4561 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4621 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4681 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4741 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4801 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4861 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4921 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4981 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5041 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5101 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5161 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5221 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5281 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5341 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5401 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5461 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5521 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5581 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5641 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5701 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5761 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5821 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5881 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5941 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6001 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6061 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6121 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6181 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6241 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6301 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6361 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6421 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6481 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6541 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6601 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6661 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6721 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6781 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6841 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6901 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6961 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7021 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7081 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7141 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7201 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7261 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7321 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7381 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7441 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7501 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7561 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7621 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7681 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7741 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7801 TAACAAAAAT TTAACGCGAA TTTT
//