LOCUS       RC223358                8547 bp    DNA     circular  SYN 
DEFINITION  NOMO2 (Myc-DDK-tagged)-Human NODAL modulator 2 (NOMO2), transcript variant 2
ACCESSION   NM_173614
VERSION     NM_173614.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4704..4795 
                     /label="Myc-DDK"  
     misc_feature    4825..5410 
                     /label="PolyA signal"  
     misc_feature    4882..4901 
                     /label="Restriction Enzyme"  
     misc_feature    5686..6358 
                     /label="ColE1"  
     misc_feature    6788..7582 
                     /label="Kan/Neo"  
     misc_feature    7617..7919 
                     /label="SV40 ori"  
     misc_feature    8005..8482 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4686..4692 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4686 
                     /label="NOMO2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGGTGGGC CAGGGCGCGG GGCTGCTGGG GCCCGCGGTG GTCACCGCCG 
     1081 CGGTGGTGCT GCTGCTGAGC GGCGTGGGGC CGGCGCACGG CTCGGAGGAC ATCGTGGTGG 
     1141 GCTGCGGTGG CTTCGTCAAG TCGGACGTGG AGATCAACTA CTCGCTCATC GAGATAAAGC 
     1201 TGTACACCAA GCATGGGACT TTGAAATACC AGACAGACTG TGCCCCTAAT AATGGTTACT 
     1261 TTATGATCCC TTTGTATGAT AAGGGGGATT TCATTCTGAA GATTGAGCCT CCCCTAGGGT 
     1321 GGAGTTTTGA GCCGACGACC GTGGAGCTCC ATGTGGATGG AGTCAGTGAC ATCTGCACAA 
     1381 AGGGTGGGGA CATCAACTTT GTCTTCACTG GGTTCTCTGT GAATGGCAAG GTCCTCAGCA 
     1441 AAGGGCAGCC CCTGGGTCCT GCGGGAGTTC AGGTGTCTCT GAGAAACACT GGGACCGAAG 
     1501 CAAAGATCCA GTCCACAGTT ACACAGCCTG GCGGAAAGTT TGCATTTTTT AAAGTTCTGC 
     1561 CTGGAGATTA TGAAATCCTC GCAACTCATC CAACCTGGGC GTTGAAAGAG GCAAGCACCA 
     1621 CAGTGCGTGT AACCAACTCC AATGCCAATG CGGCCAGTCC CCTCATAGTT GCTGGCTACA 
     1681 ATGTGTCTGG CTCTGTCCGA AGTGATGGGG AGCCCATGAA AGGGGTGAAG TTTCTTCTCT 
     1741 TTTCTTCTTT AGTAACTAAA GAGGATGTCC TGGGCTGCAA TGTCTCACCA GTGCCTGGGT 
     1801 TCCAGCCCCA AGACGAGAGT CTGGTGTATT TGTGCTACAC GGTCTCCAGA GAAGATGGCT 
     1861 CGTTCTCTTT CTATTCCTTG CCAAGTGGGG GCTACACTGT GATTCCGTTC TATCGAGGGG 
     1921 AGAGGATTAC CTTTGATGTG GCGCCTTCCA GACTTGACTT CACAGTGGAG CATGACAGCT 
     1981 TGAAAATCGA GCCCGTGTTC CACGTCATGG GATTCTCCGT CACCGGGAGG GTCTTGAACG 
     2041 GACCCGAAGG AGATGGTGTT CCAGAAGCAG TAGTCACCCT GAATAACCAA ATCAAAGTTA 
     2101 AAACAAAAGC TGATGGCTCA TTCCGCCTTG AGAACATAAC CACAGGGACA TACACCATCC 
     2161 ATGCTCAGAA AGAGCACCTC TACTTTGAAA CGGTCACCAT CAAAATTGCA CCGAACACAC 
     2221 CTCAGCTGGC TGACATTGTT GCAACAGGGT TCAGTGTCTG TGGTCGGATA TCAATCATTC 
     2281 GCTTCCCGGA CACCGTCAAG CAGATGAATA AATACAAAGT TGTCCTGTCA TCTCAAGACA 
     2341 AGGACAAGTC TTTGGTCACC GTGGAGACAG ATGCTCATGG ATCATTTTGT TTTAAAGCAA 
     2401 AACCAGGGAC TTACAAAGTG CAGGTGATGG TTCCTGAGGC AGAAACCAGA GCAGGGCTGA 
     2461 CGTTGAAACC CCAGACATTT CCTCTTACTG TGACCGACAG GCCCGTGATG GATGTGGCCT 
     2521 TTGTACAGTT CTTGGCATCA GTTTCTGGGA AAGTCTCTTG TTTGGACACC TGTGGTGACT 
     2581 TGCTGGTGAC TCTACAGTCC CTGAGCCGCC AGGGTGAGAA GCGGAGCCTC CAGCTCTCCG 
     2641 GCAAGGTCAA CGCCATGACT TTCACCTTTG ACAACGTGCT CCCTGGAAAA TACAAAATAA 
     2701 GCATCATGCA TGAGGATTGG TGCTGGAAGA ACAAGAGCCT GGAGGTGGAA GTGCTGGAGG 
     2761 ATGACGTGTC TGCAGTTGAG TTCAGGCAGA CGGGCTACAT GCTGAGATGT TCCCTGTCTC 
     2821 ACGCCATCAC TCTGGAATTT TATCAGGATG GAAATGGGCG TGAGAATGTG GGGATTTATA 
     2881 ACCTCTCCAA AGGAGTCAAC CGATTCTGCC TGTCCAAGCC TGGTGTGTAC AAAGTGACCC 
     2941 CTCGCTCCTG CCACCGGTTT GAGCAAGCGT TCTACACCTA TGACACGTCT TCACCTAGTA 
     3001 TCTTGACATT GACAGCCATT CGCCACCATG TCCTTGGAAC TATCACCACC GACAAAATGA 
     3061 TGGATGTCAC TGTGACTATC AAGTCTTCCA TCGACAGTGA ACCCGCCTTG GTCTTAGGCC 
     3121 CTCTGAAGTC TGTGCAGGAG CTGCGGAGGG AGCAGCAGCT GGCTGAGATC GAGGCCCGCA 
     3181 GGCAGGAGAG GGAGAAAAAC GGCAATGAGG AAGGTGAAGA AAGAATGACC AAGCCTCCCG 
     3241 TGCAGGAGAT GGTAGATGAG TTACAAGGCC CCTTCTCGTA TGATTTCTCT TACTGGGCGC 
     3301 GGTCTGGAGA GAAAATCACT GTTACACCGT CATCTAAAGA GCTGCTCTTT TATCCCCCTT 
     3361 CAATGGAAGC CGTTGTCAGT GGAGAAAGCT GCCCAGGGAA GCTGATCGAG ATCCACGGGA 
     3421 AGGCAGGCCT GTTTTTAGAA GGCCAGATCC ACCCCGAGTT GGAAGGAGTC GAGATTGTCA 
     3481 TCAGTGAAAA GGGGGCAAGT TCACCGCTGA TCACAGTCTT TACTGATGAC AAAGGTGCCT 
     3541 ACAGTGTTGG CCCCCTGCAC AGTGACCTGG AGTACACGGT GACCTCACAG AAGGAGGGCT 
     3601 ATGTTCTGAC TGCAGTGGAA GGAACCATCG GAGACTTCAA GGCCTATGCC CTGGCAGGCG 
     3661 TAAGCTTTGA GATAAAAGCT GAGGATGACC AGCCCCTCCC GGGAGTCCTC TTATCCCTGA 
     3721 GTGGTGGCCT GTTTCGTTCC AACCTCTTGA CCCAGGACAA CGGCATTCTG ACATTCTCAA 
     3781 ACCTGAGCCC TGGCCAGTAT TACTTCAAAC CCATGATGAA GGAGTTCCGG TTTGAGCCAT 
     3841 CCTCACAGAT GATCGAGGTG CAGGAAGGCC AGAACCTGAA GATCACCATC ACGGGGTACC 
     3901 GAACCGCTTA CAGTTGCTAT GGCACAGTGT CTTCCTTAAA CGGAGAGCCC GAACAAGGGG 
     3961 TTGCCATGGA AGCGGTGGGC CAGAACGACT GCAGCATTTA CGGAGAAGAC ACCGTGACAG 
     4021 ACGAAGAGGG CAAGTTCAGA TTACGTGGAT TGCTGCCGGG ATGTGTGTAC CACGTTCAGC 
     4081 TCAAGGCAGA AGGCAACGAC CACATTGAGC GGGCGCTCCC CCACCATAGG GTGATTGAGG 
     4141 TTGGGAATAA TGACATCGAT GATGTAAACA TCATAGTTTT CCGGCAGATT AATCAATTTG 
     4201 ATTTAAGTGG AAATGTGATC ACTTCCTCTG AATACCTTCC TACGTTATGG GTCAAGCTTT 
     4261 ACAAAAGCGA AAACCTCGAC AATCCAATCC AGACAGTTTC CCTTGGCCAG TCCCTGTTCT 
     4321 TCCATTTCCC CCCACTGCTC AGAGATGGCG AGAACTATGT TGTGCTTCTG GACTCCACAC 
     4381 TCCCCAGATC CCAGTATGAC TACATCTTGC CTCAAGTTTC TTTCACCGCA GTGGGCTACC 
     4441 ATAAACACAT CACCTTGATT TTTAATCCCA CGAGGAAGCT GCCTGAACAG GACATCGCAC 
     4501 AAGGATCCTA CATTGCCCTG CCATTGACGC TGCTGGTTCT GCTGGCCGGT TACAACCATG 
     4561 ACAAGCTCAT TCCTTTGCTG CTGCAGTTGA CAAGCCGGCT ACAGGGAGTC GGCGCGCTCG 
     4621 GCCAGGCAGC CTCTGACAAT AGCGGCCCAG AAGATGCAAA GAGACAAGCC AAGAAACAGA 
     4681 AGACAAGGCG GACGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4741 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4801 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4861 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4921 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4981 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5041 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5101 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5161 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5221 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5281 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5341 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5401 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5461 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5521 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5581 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5641 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5701 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5761 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5821 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5881 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5941 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6001 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6061 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6121 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6181 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6241 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6301 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6361 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6421 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6481 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6541 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6601 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6661 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6721 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6781 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6841 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6901 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6961 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7021 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7081 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7141 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7201 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7261 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7321 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7381 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7441 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7501 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7561 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7621 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7681 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7741 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7801 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7861 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7921 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7981 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8041 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8101 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8161 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8221 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8281 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8341 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8401 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8461 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8521 ATTTAACAAA AATTTAACGC GAATTTT
//