LOCUS       RC223329                6642 bp    DNA     circular  SYN 
DEFINITION  BCAS1 (Myc-DDK-tagged)-Human breast carcinoma amplified sequence 1 (BCAS1)
ACCESSION   NM_003657
VERSION     NM_003657.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2797..2888 
                     /label="Myc-DDK"  
     misc_feature    2918..3503 
                     /label="PolyA signal"  
     misc_feature    2975..2994 
                     /label="Restriction Enzyme"  
     misc_feature    3779..4451 
                     /label="ColE1"  
     misc_feature    4881..5675 
                     /label="Kan/Neo"  
     misc_feature    5710..6012 
                     /label="SV40 ori"  
     misc_feature    6098..6575 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2772..2779 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2772 
                     /label="BCAS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTAACCAA ATGAGTGTTC CCCAAAGAGT TGAAGACCAA GAGAATGAAC 
     1081 CAGAAGCAGA GACTTACCAG GACAACGCGT CTGCTCTGAA CGGGGTTCCA GTGGTGGTGT 
     1141 CGACCCACAC AGTTCAGCAC TTAGAGGAAG TCGACTTGGG AATAAGTGTC AAGACGGATA 
     1201 ATGTGGCCAC TTCTTCCCCC GAGACAACGG AGATAAGTGC TGTTGCGGAT GCCAACGGAA 
     1261 AGAATCTTGG GAAAGAGGCC AAACCCGAGG CACCAGCTGC TAAATCTCGT TTTTTCTTGA 
     1321 TGCTCTCTCG GCCTGTACCA GGACGTACCG GAGACCAAGC CGCAGATTCA TCCCTTGGAT 
     1381 CAGTGAAGCT TGATGTCAGC TCCAATAAAG CTCCAGCGAA CAAAGACCCA AGTGAGAGCT 
     1441 GGACACTTCC GGTGGCAGCT GGACCGGGGC AGGACACAGA TAAAACCCCA GGGCACGCCC 
     1501 CGGCCCAAGA CAAGGTCCTC TCTGCCGCCA GGGATCCCAC GCTTCTCCCA CCTGAGACAG 
     1561 GGGGAGCAGG AGGAGAAGCT CCCTCCAAGC CCAAGGACTC CAGCTTTTTT GACAAATTCT 
     1621 TCAAGCTGGA CAAGGGACAG GAAAAGGTGC CAGGTGACAG CCAACAGGAA GCCAAGAGGG 
     1681 CAGAGCATCA AGACAAGGTG GATGAGGTTC CTGGCTTATC AGGGCAGTCC GATGATGTCC 
     1741 CTGCAGGGAA GGACATAGTT GACGGCAAGG AAAAAGAAGG ACAAGAACTT GGAACTGCGG 
     1801 ATTGCTCTGT CCCTGGGGAC CCAGAAGGAC TGGAGACTGC AAAGGACGAT TCCCAGGCAG 
     1861 CAGCTATAGC AGAGAATAAT AATTCTATCA TGAGTTTCTT TAAAACTCTG GTTTCACCTA 
     1921 ACAAAGCTGA AACAAAAAAG GACCCAGAAG ACACGGGTGC TGAAAAGTCA CCCACCACTT 
     1981 CAGCTGACCT TAAGTCAGAC AAAGCCAACT TTACATCCCA GGAGACCCAA GGGGCTGGCA 
     2041 AGAATTCCAA AGGATGCAAC CCATCGGGGC ACACACAGTC CGTGACAACC CCTGAACCTG 
     2101 CGAAGGAAGG CACCAAGGAG AAATCAGGAC CCACCTCTCT GCCTCTGGGC AAACTGTTTT 
     2161 GGAAAAAGTC AGTTAAAGAG GACTCAGTCC CCACAGGTGC GGAGGAGAAT GTGGTGTGTG 
     2221 AGTCACCAGT AGAGATTATA AAGTCCAAGG AAGTAGAATC AGCCTTACAA ACAGTGGACC 
     2281 TCAACGAAGG AGATGCTGCA CCTGAACCCA CAGAAGCGAA ACTCAAAAGA GAAGAAAGCA 
     2341 AACCAAGAAC CTCTCTGATG GCGTTTCTCA GACAAATGTC AGTGAAAGGG GATGGAGGGA 
     2401 TCACCCACTC AGAAGAAATA AATGGGAAAG ACTCCAGCTG CCAAACATCA GACTCCACAG 
     2461 AAAAGACTAT CACACCGCCA GAGCCTGAAC CAACAGGAGC ACCACAGAAG GGTAAAGAGG 
     2521 GCTCCTCGAA GGACAAGAAG TCAGCAGCCG AGATGAACAA GCAGAAGAGC AACAAGCAGG 
     2581 AAGCCAAAGA ACCAGCCCAG TGCACAGAGC AGGCCACGGT GGACACGAAC TCACTGCAGA 
     2641 ATGGGGACAA GCTCCAAAAG AGACCTGAGA AGCGGCAGCA GTCCCTTGGG GGCTTCTTTA 
     2701 AAGGCCTGGG ACCAAAGCGG ATGTTGGATG CTCAAGTGCA AACAGACCCA GTATCCATCG 
     2761 GACCAGTTGG CAAACCCAAG AGCGGACCGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     2821 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     2881 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     2941 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3001 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3061 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3121 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3181 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3241 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3301 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3361 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3421 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3481 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3541 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3601 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3661 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3721 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     3781 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     3841 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     3901 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     3961 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4021 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4081 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4141 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4201 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4261 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4321 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4381 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4441 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4501 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4561 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4621 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4681 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4741 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     4801 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     4861 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     4921 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     4981 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5041 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5101 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5161 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5221 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5281 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5341 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5401 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5461 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5521 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5581 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5641 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5701 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     5761 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     5821 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     5881 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     5941 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6001 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6061 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6121 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6181 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6241 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6301 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6361 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6421 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6481 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6541 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6601 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//