LOCUS       RC223225                8397 bp    DNA     circular  SYN 
DEFINITION  THBS2 (Myc-DDK-tagged)-Human thrombospondin 2 (THBS2)
ACCESSION   NM_003247
VERSION     NM_003247.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4554..4645 
                     /label="Myc-DDK"  
     misc_feature    4675..5260 
                     /label="PolyA signal"  
     misc_feature    4732..4751 
                     /label="Restriction Enzyme"  
     misc_feature    5536..6208 
                     /label="ColE1"  
     misc_feature    6638..7432 
                     /label="Kan/Neo"  
     misc_feature    7467..7769 
                     /label="SV40 ori"  
     misc_feature    7855..8332 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4536..4542 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4536 
                     /label="THBS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCTGGAGG CTGGTCCTGC TGGCTCTGTG GGTGTGGCCC AGCACGCAAG 
     1081 CTGGTCACCA GGACAAAGAC ACGACCTTCG ACCTTTTCAG TATCAGCAAC ATCAACCGCA 
     1141 AGACCATTGG CGCCAAGCAG TTCCGCGGGC CCGACCCCGG CGTGCCGGCT TACCGCTTCG 
     1201 TGCGCTTTGA CTACATCCCA CCGGTGAACG CAGATGACCT CAGCAAGATC ACCAAGATCA 
     1261 TGCGGCAGAA GGAGGGCTTC TTCCTCACGG CCCAGCTCAA GCAGGACGGC AAGTCCAGGG 
     1321 GCACGCTGTT GGCTCTGGAG GGCCCCGGTC TCTCCCAGAG GCAGTTCGAG ATCGTCTCCA 
     1381 ACGGCCCCGC GGACACGCTG GATCTCACCT ACTGGATTGA CGGCACCCGG CATGTGGTCT 
     1441 CCCTGGAGGA CGTCGGCCTG GCTGACTCGC AGTGGAAGAA CGTCACCGTG CAGGTGGCTG 
     1501 GCGAGACCTA CAGCTTGCAC GTGGGCTGCG ACCTCATAGA CAGCTTCGCT CTGGACGAGC 
     1561 CCTTCTACGA GCACCTGCAG GCGGAAAAGA GCCGGATGTA CGTGGCCAAA GGCTCTGCCA 
     1621 GAGAGAGTCA CTTCAGGGGT TTGCTTCAGA ACGTCCACCT AGTGTTTGAA AACTCTGTGG 
     1681 AAGATATTCT AAGCAAGAAG GGTTGCCAGC AAGGCCAGGG AGCTGAGATC AACGCCATCA 
     1741 GTGAGAACAC AGAGACGCTG CGCCTGGGTC CGCATGTCAC CACCGAGTAC GTGGGCCCCA 
     1801 GCTCGGAGAG GAGGCCCGAG GTGTGCGAAC GCTCGTGCGA GGAGCTGGGA AACATGGTCC 
     1861 AGGAGCTCTC GGGGCTCCAC GTCCTCGTGA ACCAGCTCAG CGAGAACCTC AAGAGAGTGT 
     1921 CGAATGATAA CCAGTTTCTC TGGGAGCTCA TTGGTGGCCC TCCTAAGACA AGGAACATGT 
     1981 CAGCTTGCTG GCAGGATGGC CGGTTCTTTG CGGAAAATGA AACGTGGGTG GTGGACAGCT 
     2041 GCACCACGTG TACCTGCAAG AAATTTAAAA CCATTTGCCA CCAAATCACC TGCCCGCCTG 
     2101 CAACCTGCGC CAGTCCATCC TTTGTGGAAG GCGAATGCTG CCCTTCCTGC CTCCACTCGG 
     2161 TGGACGGTGA GGAGGGCTGG TCTCCGTGGG CAGAGTGGAC CCAGTGCTCC GTGACGTGTG 
     2221 GCTCTGGGAC CCAGCAGAGA GGCCGGTCCT GTGACGTCAC CAGCAACACC TGCTTGGGGC 
     2281 CCTCCATCCA GACACGGGCT TGCAGTCTGA GCAAGTGTGA CACCCGCATC CGGCAGGACG 
     2341 GCGGCTGGAG CCACTGGTCA CCTTGGTCTT CATGCTCTGT GACCTGTGGA GTTGGCAATA 
     2401 TCACACGCAT CCGTCTCTGC AACTCCCCAG TGCCCCAGAT GGGGGGCAAG AATTGCAAAG 
     2461 GGAGTGGCCG GGAGACCAAA GCCTGCCAGG GCGCCCCATG CCCAATCGAT GGCCGCTGGA 
     2521 GCCCCTGGTC CCCGTGGTCG GCCTGCACTG TCACCTGTGC CGGTGGGATC CGGGAGCGCA 
     2581 CCCGGGTCTG CAACAGCCCT GAGCCTCAGT ACGGAGGGAA GGCCTGCGTG GGGGATGTGC 
     2641 AGGAGCGTCA GATGTGCAAC AAGAGGAGCT GCCCCGTGGA TGGCTGTTTA TCCAACCCCT 
     2701 GCTTCCCGGG AGCCCAGTGC AGCAGCTTCC CCGATGGGTC CTGGTCATGC GGCTCCTGCC 
     2761 CTGTGGGCTT CTTGGGCAAT GGCACCCACT GTGAGGACCT GGACGAGTGT GCCCTGGTCC 
     2821 CCGACATCTG CTTCTCCACC AGCAAGGTGC CTCGCTGTGT CAACACTCAG CCTGGCTTCC 
     2881 ACTGCCTGCC CTGCCCGCCC CGATACAGAG GGAACCAGCC CGTCGGGGTC GGCCTGGAAG 
     2941 CAGCCAAGAC GGAAAAGCAA GTGTGTGAGC CCGAAAACCC ATGCAAGGAC AAGACACACA 
     3001 ACTGCCACAA GCACGCGGAG TGCATCTACC TGGGCCACTT CAGCGACCCC ATGTACAAGT 
     3061 GCGAGTGCCA GACAGGCTAC GCGGGCGACG GGCTCATCTG CGGGGAGGAC TCGGACCTGG 
     3121 ACGGCTGGCC CAACCTCAAT CTGGTCTGCG CCACCAACGC CACCTACCAC TGCATCAAGG 
     3181 ATAACTGCCC CCATCTGCCA AATTCTGGGC AGGAAGACTT TGACAAGGAC GGGATTGGCG 
     3241 ATGCCTGTGA TGATGACGAT GACAATGACG GTGTGACCGA TGAGAAGGAC AACTGCCAGC 
     3301 TCCTCTTCAA TCCCCGCCAG GCTGACTATG ACAAGGATGA GGTTGGGGAC CGCTGTGACA 
     3361 ACTGCCCTTA CGTGCACAAC CCTGCCCAGA TCGACACAGA CAACAATGGA GAGGGTGACG 
     3421 CCTGCTCCGT GGACATTGAT GGGGACGATG TCTTCAATGA ACGAGACAAT TGTCCCTACG 
     3481 TCTACAACAC TGACCAGAGG GACACGGATG GTGACGGTGT GGGGGATCAC TGTGACAACT 
     3541 GCCCCCTGGT GCACAACCCT GACCAGACCG ACGTGGACAA TGACCTTGTT GGGGACCAGT 
     3601 GTGACAACAA CGAGGACATA GATGACGACG GCCACCAGAA CAACCAGGAC AACTGCCCCT 
     3661 ACATCTCCAA CGCCAACCAG GCTGACCATG ACAGAGACGG CCAGGGCGAC GCCTGTGACC 
     3721 CTGATGATGA CAACGATGGC GTCCCCGATG ACAGGGACAA CTGCCGGCTT GTGTTCAACC 
     3781 CAGACCAGGA GGACTTGGAC GGTGATGGAC GGGGTGATAT TTGTAAAGAT GATTTTGACA 
     3841 ATGACAACAT CCCAGATATT GATGATGTGT GTCCTGAAAA CAATGCCATC AGTGAGACAG 
     3901 ACTTCAGGAA CTTCCAGATG GTCCCCTTGG ATCCCAAAGG GACCACCCAA ATTGATCCCA 
     3961 ACTGGGTCAT TCGCCATCAA GGCAAGGAGC TGGTTCAGAC AGCCAACTCG GACCCCGGCA 
     4021 TCGCTGTAGG TTTTGACGAG TTTGGGTCTG TGGACTTCAG TGGCACATTC TACGTAAACA 
     4081 CTGACCGGGA CGACGACTAT GCCGGCTTCG TCTTTGGTTA CCAGTCAAGC AGCCGCTTCT 
     4141 ATGTGGTGAT GTGGAAGCAG GTGACGCAGA CCTACTGGGA GGACCAGCCC ACGCGGGCCT 
     4201 ATGGCTACTC CGGCGTGTCC CTCAAGGTGG TGAACTCCAC CACGGGGACG GGCGAGCACC 
     4261 TGAGGAACGC GCTGTGGCAC ACGGGGAACA CGCCGGGGCA GGTGCGAACC TTATGGCACG 
     4321 ACCCCAGGAA CATTGGCTGG AAGGACTACA CGGCCTATAG GTGGCACCTG ACTCACAGGC 
     4381 CCAAGACTGG CTACATCAGA GTCTTAGTGC ATGAAGGAAA ACAGGTCATG GCAGACTCAG 
     4441 GACCTATCTA TGACCAAACC TACGCTGGCG GGCGGCTGGG TCTATTTGTC TTCTCTCAAG 
     4501 AAATGGTCTA TTTCTCAGAC CTCAAGTACG AATGCAGAGA TATTACGCGT ACGCGGCCGC 
     4561 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4621 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4681 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4741 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4801 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4861 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4921 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4981 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5041 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5101 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5161 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5221 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5281 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5341 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5401 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5461 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5521 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5581 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5641 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5701 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5761 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5821 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5881 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5941 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6001 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6061 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6121 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6181 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6241 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6301 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6361 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6421 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6481 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6541 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6601 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6661 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6721 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6781 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6841 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6901 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6961 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7021 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7081 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7141 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7201 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7261 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7321 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7381 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7441 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7501 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7561 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7621 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7801 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7861 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7921 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7981 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8041 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8101 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8161 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8221 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8281 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8341 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//