LOCUS       RC223204                7476 bp    DNA     circular  SYN 
DEFINITION  TOP3B (Myc-DDK-tagged)-Human topoisomerase (DNA) III beta (TOP3B)
ACCESSION   NM_003935
VERSION     NM_003935.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3631..3722 
                     /label="Myc-DDK"  
     misc_feature    3752..4337 
                     /label="PolyA signal"  
     misc_feature    3809..3828 
                     /label="Restriction Enzyme"  
     misc_feature    4613..5285 
                     /label="ColE1"  
     misc_feature    5715..6509 
                     /label="Kan/Neo"  
     misc_feature    6544..6846 
                     /label="SV40 ori"  
     misc_feature    6932..7409 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3606..3613 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3606 
                     /label="TOP3B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGACTGTG CTCATGGTTG CTGAAAAGCC GTCCTTGGCA CAGTCAATTG 
     1081 CCAAAATCCT CTCTAGAGGG AGCCTGTCCT CACACAAAGG GCTGAACGGG GCCTGCTCAG 
     1141 TCCACGAGTA CACTGGGACC TTTGCTGGCC AGCCAGTGCG CTTCAAGATG ACGTCTGTCT 
     1201 GTGGTCACGT GATGACCCTG GATTTCCTGG GAAAATACAA CAAATGGGAC AAAGTGGACC 
     1261 CCGCAGAACT GTTCAGCCAA GCTCCCACGG AGAAGAAAGA AGCTAACCCC AAGCTGAACA 
     1321 TGGTGAAGTT CCTGCAGGTG GAGGGCAGAG GCTGCGACTA CATCGTGCTG TGGCTGGACT 
     1381 GCGACAAGGA GGGGGAGAAC ATCTGCTTTG AGGTTCTTGA TGCTGTTCTG CCCGTCATGA 
     1441 ACAAGGCCCA TGGTGGCGAG AAGACCGTGT TCCGGGCCAG GTTTAGCTCC ATCACGGACA 
     1501 CAGACATCTG TAATGCCATG GCCTGCCTAG GCGAGCCTGA CCACAACGAG GCGCTCTCAG 
     1561 TGGATGCTCG CCAGGAGCTG GACCTGCGAA TCGGCTGTGC ATTCACCAGG TTTCAGACTA 
     1621 AATATTTCCA GGGGAAATAC GGTGATTTAG ACAGCTCTCT CATCTCCTTT GGGCCGTGTC 
     1681 AGACTCCAAC CCTGGGATTC TGTGTGGAGA GACATGATAA AATCCAGTCC TTCAAACCAG 
     1741 AGACCTACTG GGTGCTGCAG GCCAAGGTTA ACACTGACAA AGACAGATCT CTCCTTTTGG 
     1801 ACTGGGACCG AGTAAGAGTG TTTGACCGGG AGATCGCACA GATGTTTTTA AACATGACAA 
     1861 AGCTGGAGAA GGAAGCCCAG GTGGAGGCCA CAAGCAGGAA AGAAAAGGCC AAGCAGAGGC 
     1921 CCCTGGCCCT GAACACTGTG GAGATGCTGC GTGTGGCCAG CTCTTCTCTG GGCATGGGGC 
     1981 CGCAGCACGC CATGCAGACG GCTGAGCGGC TCTACACGCA AGGCTACATC AGCTACCCAC 
     2041 GGACAGAGAC CACCCACTAC CCTGAGAACT TTGACCTGAA GGGCTCTCTG CGGCAGCAGG 
     2101 CCAACCACCC CTACTGGGCC GACACGGTGA AGCGGTTGTT AGCAGAAGGT ATCAACCGCC 
     2161 CGCGGAAAGG CCATGACGCC GGCGACCATC CCCCCATCAC CCCCATGAAG TCTGCCACAG 
     2221 AGGCCGAATT AGGGGGTGAC GCGTGGCGGC TCTATGAGTA CATCACCAGA CACTTCATCG 
     2281 CCACGGTCAG CCATGACTGC AAGTACCTGC AGAGCACCAT CTCCTTCAGA ATTGGGCCCG 
     2341 AGCTCTTCAC CTGCTCCGGG AAGACCGTCC TCTCACCAGG CTTCACGGAG GTCATGCCCT 
     2401 GGCAGAGCGT GCCCCTGGAG GAGAGCCTGC CCACTTGCCA GCGGGGTGAT GCCTTCCCTG 
     2461 TGGGCGAGGT GAAGATGCTG GAGAAGCAGA CGAACCCACC CGACTACCTG ACGGAGGCCG 
     2521 AGCTCATCAC GCTCATGGAG AAGCATGGCA TCGGCACGGA TGCCAGCATC CCTGTGCATA 
     2581 TCAACAACAT CTGCCAGCGC AACTATGTCA CGGTGGAGAG CGGGCGCCGG CTCAAGCCCA 
     2641 CCAACCTCGG CATCGTCCTG GTGCACGGCT ACTATAAGAT TGATGCAGAG CTGGTGCTCC 
     2701 CCACCATCCG CAGTGCAGTG GAGAAGCAGC TGAACCTGAT CGCCCAGGGC AAGGCCGACT 
     2761 ACCGCCAGGT CCTGGGCCAC ACCCTGGACG TGTTCAAGAG GAAGTTCCAC TACTTTGTCG 
     2821 ACTCCATTGC TGGCATGGAT GAGTTGATGG AGGTGTCTTT CTCGCCCCTG GCGGCCACAG 
     2881 GCAAGCCCCT CTCACGCTGT GGGAAGTGCC ACCGCTTCAT GAAGTACATC CAGGCCAAGC 
     2941 CAAGCCGCCT GCACTGCTCC CACTGCGATG AGACCTACAC GCTCCCCCAG AACGGCACCA 
     3001 TCAAGCTCTA CAAGGAGCTC CGCTGCCCTC TGGATGACTT CGAGCTGGTC CTGTGGTCAT 
     3061 CAGGCTCTCG GGGCAAGAGC TACCCGCTGT GCCCCTACTG CTACAACCAC CCACCCTTCC 
     3121 GAGACATGAA GAAAGGCATG GGCTGCAACG AGTGTACGCA CCCCTCCTGC CAGCACTCGC 
     3181 TGAGCATGCT GGGCATCGGC CAGTGCGTGG AATGTGAGAG CGGGGTGCTG GTGCTGGACC 
     3241 CCACCTCGGG CCCCAAGTGG AAGGTGGCCT GCAACAAGTG CAACGTGGTA GCGCACTGCT 
     3301 TCGAGAACGC CCACCGCGTG CGGGTGTCCG CCGACACCTG CAGTGTCTGT GAGGCCGCCT 
     3361 TGCTTGATGT GGACTTCAAC AAGGCCAAGT CCCCACTCCC GGGCGATGAG ACGCAGCACA 
     3421 TGGGCTGCGT CTTTTGTGAC CCCGTCTTCC AGGAGCTGGT GGAGCTGAAG CATGCGGCCT 
     3481 CCTGCCACCC CATGCACCGC GGTGGACCAG GGAGAAGGCA GGGTCGAGGG CGGGGCCGGG 
     3541 CCAGGAGGCC CCCTGGGAAG CCCAACCCCA GACGGCCCAA GGACAAGATG TCAGCCCTGG 
     3601 CCGCCTACTT TGTAAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3661 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3721 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3781 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3841 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3901 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3961 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4021 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4081 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4141 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4201 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4261 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4321 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4381 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4441 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4501 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4561 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4621 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4681 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4741 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4801 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4861 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4921 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4981 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5041 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5101 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5161 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5221 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5281 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5341 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5401 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5461 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5521 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5581 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5641 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5701 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5761 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5821 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5881 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5941 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6001 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6061 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6121 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6181 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6241 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6301 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6361 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6421 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6481 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6541 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6601 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6661 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6721 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6781 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6841 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6901 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6961 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7021 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7081 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7141 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7201 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7261 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7321 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7381 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7441 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//