LOCUS       RC223164                8349 bp    DNA     circular  SYN 
DEFINITION  CILP2 (Myc-DDK-tagged)-Human cartilage intermediate layer protein 2 (CILP2)
ACCESSION   NM_153221
VERSION     NM_153221.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4506..4597 
                     /label="Myc-DDK"  
     misc_feature    4627..5212 
                     /label="PolyA signal"  
     misc_feature    4684..4703 
                     /label="Restriction Enzyme"  
     misc_feature    5488..6160 
                     /label="ColE1"  
     misc_feature    6590..7384 
                     /label="Kan/Neo"  
     misc_feature    7419..7721 
                     /label="SV40 ori"  
     misc_feature    7807..8284 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4488..4494 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4488 
                     /label="CILP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGTCGCTG CTGCCACTGC TCTGTCTCTG TGTCGTCGCT GCGCACCTGG 
     1081 CGGGGGCCCG AGACGCCACC CCCACCGAGG AGCCAATGGC GACTGCACTG GGCCTGGAAA 
     1141 GACGGTCCGT GTACACCGGC CAGCCCTCAC CAGCCCTGGA GGACTGGGAA GAGGCCAGCG 
     1201 AGTGGACGTC CTGGTTCAAC GTGGACCACC CCGGAGGCGA CGGCGACTTC GAGAGCCTGG 
     1261 CTGCCATCCG CTTCTACTAC GGGCCAGCGC GCGTGTGCCC GCGACCGCTG GCGCTGGAAG 
     1321 CGCGCACCAC GGACTGGGCC CTGCCGTCCG CCGTCGGCGA GCGCGTGCAC TTGAACCCCA 
     1381 CGCGCGGCTT CTGGTGCCTC AACCGCGAGC AACCGCGTGG CCGCCGCTGC TCCAACTACC 
     1441 ACGTGCGCTT CCGCTGCCCA CTAGAAGCCT CGTGGGGCGC GTGGGGCCCG TGGGGTCCCT 
     1501 GCTCGGGGAG CTGTGGGCCA GGCCGTCGCT TGCGCCGCCG CCACTGCCCA AGCCCCGCTG 
     1561 GGGATGCGTG TCCCGGGCGT CCTCTGGAGG CGCAGAAGTG CGTGCGGCCT CGGTGTCCAG 
     1621 GGTGCAGCCT TGACACCTGT GAATGCCCGG ACCACATCCT CCTGGGCTCG GTGGTCACCC 
     1681 CATCTGGGCA ACCACTGCTA GGAGCCAGGG TCTCCCTGCG AGACCAGCCT GGCACTGTGG 
     1741 CCACCAGCGA TGCTCACGGA ACCTTCCGGG TGCCTGGTGT CTGTGCTGAC AGCCGCGCCA 
     1801 ACATCAGGGC CCAGATGGAT GGCTTCTCTG CAGGGGAGGC CCAGGCCCAG GCCAACGGAT 
     1861 CCATCTCTGT GGTCACCATC ATCCTTGATA AGTTGGAGAA GCCGTACCTG GTGAAACACC 
     1921 CTGAGTCCCG AGTGCGAGAG GCTGGCCAGA ATGTGACTTT CTGCTGCAAA GCCTCCGGGA 
     1981 CCCCCATGCC CAAGAAATAC TCCTGGTTCC ACAATGGGAC CCTGCTGGAC AGGCGAGCTC 
     2041 ATGGGTACGG GGCCCACCTG GAGCTGCGGG GACTGCGCCC AGACCAGGCT GGCATCTACC 
     2101 ACTGCAAGGC ATGGAATGAG GCGGGTGCCG TGCGCTCGGG CACTGCCCGG CTCACTGTAC 
     2161 TTGCCCCAGG CCAGCCAGCC TGCGACCCCC GGCCCCGAGA GTACCTGATC AAGCTCCCTG 
     2221 AGGACTGTGG TCAGCCAGGT AGTGGCCCTG CCTACCTGGA TGTGGGCCTC TGTCCCGACA 
     2281 CCCGCTGCCC CAGCCTGGCA GGCTCCAGCC CCCGCTGCGG GGACGCCAGC TCCCGCTGCT 
     2341 GCTCTGTGCG CCGTCTGGAG AGAAGGGAGA TTCACTGCCC TGGCTACGTC CTCCCAGTGA 
     2401 AGGTGGTGGC AGAGTGTGGC TGCCAGAAGT GTCTGCCCCC TCGGGGGCTG GTCCGGGGCC 
     2461 GTGTTGTGGC TGCTGACTCC GGGGAGCCGC TACGCTTCGC CAGGATTCTG CTGGGCCAGG 
     2521 AGCCCATCGG CTTCACCGCC TACCAGGGCG ACTTTACCAT TGAGGTGCCG CCCTCCACCC 
     2581 AGCGGCTGGT GGTGACTTTT GTGGACCCCA GCGGTGAGTT CATGGACGCT GTCCGGGTCT 
     2641 TGCCTTTTGA TCCTCGAGGT GCCGGCGTGT ACCACGAGGT CAAGGCCATG CGGAAGAAAG 
     2701 CCCCGGTCAT TTTACATACC AGCCAGAGCA ACACGATCCC CCTGGGCGAG CTGGAAGATG 
     2761 AGGCGCCCCT GGGCGAGCTG GTCCTGCCTT CTGGCGCTTT CCGCAGAGCC GACGGCAAAC 
     2821 CCTACTCGGG GCCTGTGGAG GCCCGGGTGA CGTTCGTGGA CCCCCGAGAC CTCACCTCGG 
     2881 CGGCGTCTGC CCCCAGTGAC CTGCGCTTCG TGGACAGCGA CGGCGAGCTG GCTCCACTGC 
     2941 GCACCTACGG CATGTTCTCC GTGGACCTCC GTGCGCCCGG CTCCGCGGAG CAGCTGCAGG 
     3001 TGGGGCCGGT GGCCGTGCGG GTGGCCGCCA GCCAGATCCA CATGCCAGGC CACGTGGAGG 
     3061 CCCTCAAGCT GTGGTCGCTG AACCCCGAGA CCGGCTTGTG GGAGGAGGAG AGCGGCTTCC 
     3121 GGCGCGAGGG GTCCTCGGGC CCCCGGGTGC GCCGGGAGGA GCGCGTCTTC CTGGTGGGCA 
     3181 ACGTGGAGAT CCGGGAGCGG CGCCTGTTCA ATCTGGACGT GCCTGAGCGC CGCCGCTGCT 
     3241 TCGTGAAGGT GCGCGCCTAC GCCAACGACA AGTTCACCCC CAGCGAGCAG GTGGAGGGCG 
     3301 TGGTGGTCAC GCTGGTCAAT CTGGAGCCCG CCCCCGGCTT CTCCGCCAAC CCCCGTGCCT 
     3361 GGGGCCGCTT TGACAGCGCG GTCACCGGCC CCAATGGCGC CTGCCTCCCC GCCTTCTGCG 
     3421 ACGCCGACAG GCCAGACGCC TACACCGCCC TGGTCACCGC CACCCTGGGC GGCGAGGAGC 
     3481 TGGAGCCGGC CCCTTCCTTG CCCCGCCCAC TCCCGGCCAC CGTGGGCGTC ACCCAGCCCT 
     3541 ACCTGGACAG GCTGGGGTAC CGTCGGACGG ACCACGACGA TCCCGCCTTC AAGCGTAACG 
     3601 GCTTCCGCAT CAACCTCGCC AAGCCCAGGC CAGGTGACCC CGCCGAGGCC AATGGGCCTG 
     3661 TGTACCCGTG GCGCAGCCTG CGGGAATGCC AGGGGGCCCC GGTGACTGCC AGCCACTTCC 
     3721 GCTTCGCCAG GGTGGAGGCG GACAAGTACG AGTACAACGT GGTCCCCTTC CGAGAGGGCA 
     3781 CACCTGCCTC CTGGACTGGC GATCTCCTGG CCTGGTGGCC CAACCCGCAG GAGTTCCGGG 
     3841 CCTGCTTCCT CAAGGTGAAG ATCCAGGGTC CCCAGGAGTA TATGGTCCGC TCCCACAACG 
     3901 CAGGGGGCAG CCACCCACGC ACCCGCGGCC AGCTCTACGG ACTTCGGGAT GCCCGGAGTG 
     3961 TGCGAGACCC CGAGCGTCCG GGCACCTCGG CAGCCTGCGT GGAGTTCAAG TGCAGCGGGA 
     4021 TGCTGTTCGA CCAGCGGCAG GTGGACAGGA CGCTGGTGAC CATTATGCCC CAGGGCAGCT 
     4081 GCCGGCGCGT GGCCGTCAAC GGACTCCTTC GGGATTACCT GACCCGGCAC CCCCCACCGG 
     4141 TGCCCGCGGA GGACCCAGCT GCCTTCTCCA TGCTGGCCCC CCTAGACCCT CTGGGCCACA 
     4201 ACTATGGCGT CTACACTGTC ACTGACCAGA GCCCACGCTT GGCCAAGGAG ATCGCCATTG 
     4261 GCCGCTGCTT TGATGGTTCC TCTGACGGCT TCTCCAGAGA GATGAAGGCT GATGCCGGCA 
     4321 CAGCCGTCAC CTTCCAGTGC CGGGAGCCAC CGGCCGGACG ACCCAGCCTC TTCCAGAGGC 
     4381 TGCTGGAGTC CCCGGCGACA GCACTTGGTG ACATCCGCAG GGAGATGAGC GAGGCGGCGC 
     4441 AGGCACAGGC CCGGGCCTCA GGTCCCCTCC GCACCCGCCG GGGTAGGGTC CGGCAGACGC 
     4501 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4561 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4621 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4681 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4741 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4801 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4861 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4921 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4981 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5041 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5101 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5161 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5221 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5281 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5341 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5401 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5461 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5521 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5581 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5641 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5701 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5761 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5821 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5881 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5941 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6001 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6061 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6121 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6181 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6241 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6301 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6361 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6421 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6481 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6541 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6601 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6661 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6721 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6781 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6841 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6901 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6961 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7021 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7081 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7141 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7201 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7261 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7321 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7381 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7441 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7501 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7561 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7801 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7861 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7921 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7981 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8041 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8101 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8161 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8221 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8281 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8341 GCGAATTTT
//