LOCUS       RC223041                8433 bp    DNA     circular  SYN 
DEFINITION  PCDH12 (Myc-DDK-tagged)-Human protocadherin 12 (PCDH12)
ACCESSION   NM_016580
VERSION     NM_016580.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4590..4681 
                     /label="Myc-DDK"  
     misc_feature    4711..5296 
                     /label="PolyA signal"  
     misc_feature    4768..4787 
                     /label="Restriction Enzyme"  
     misc_feature    5572..6244 
                     /label="ColE1"  
     misc_feature    6674..7468 
                     /label="Kan/Neo"  
     misc_feature    7503..7805 
                     /label="SV40 ori"  
     misc_feature    7891..8368 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4572..4578 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4572 
                     /label="PCDH12"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCAACTT CTGCAACTTC TGCTGGGGCT TTTGGGGCCA GGTGGCTACT 
     1081 TATTTCTTTT AGGGGATTGT CAGGAGGTGA CCACTCTCAC GGTGAAATAC CAAGTGTCAG 
     1141 AGGAAGTGCC ATCTGGTACA GTGATCGGGA AGCTGTCCCA GGAACTGGGC CGGGAGGAGA 
     1201 GGCGGAGGCA AGCTGGGGCT GCCTTCCAGG TGTTGCAGCT GCCTCAGGCG CTCCCCATTC 
     1261 AGGTGGACTC TGAGGAAGGC TTGCTCAGCA CAGGCAGGCG GCTGGATCGA GAGCAGCTGT 
     1321 GCCGACAGTG GGATCCCTGC CTGGTTTCCT TTGATGTGCT TGCCACAGGG GATTTGGCTC 
     1381 TGATCCATGT GGAGATCCAA GTGCTGGACA TCAATGACCA CCAGCCACGG TTTCCCAAAG 
     1441 GCGAGCAGGA GCTGGAAATC TCTGAGAGCG CCTCTCTGCG AACCCGGATC CCCCTGGACA 
     1501 GAGCTCTTGA CCCAGACACA GGCCCTAACA CCCTGCACAC CTACACTCTG TCTCCCAGTG 
     1561 AGCACTTTGC CTTGGATGTC ATTGTGGGCC CTGATGAGAC CAAACATGCA GAACTCATAG 
     1621 TGGTGAAGGA GCTGGACAGG GAAATCCATT CATTTTTTGA TCTGGTGTTA ACTGCCTATG 
     1681 ACAATGGGAA CCCCCCCAAG TCAGGTACCA GCTTGGTCAA GGTCAACGTC TTGGACTCCA 
     1741 ATGACAATAG CCCTGCGTTT GCTGAGAGTT CACTGGCACT GGAAATCCAA GAAGATGCTG 
     1801 CACCTGGTAC GCTTCTCATA AAACTGACCG CCACAGACCC TGACCAAGGC CCCAATGGGG 
     1861 AGGTGGAGTT CTTCCTCAGT AAGCACATGC CTCCAGAGGT GCTGGACACC TTCAGTATTG 
     1921 ATGCCAAGAC AGGCCAGGTC ATTCTGCGTC GACCTCTAGA CTATGAAAAG AACCCTGCCT 
     1981 ACGAGGTGGA TGTTCAGGCA AGGGACCTGG GTCCCAATCC TATCCCAGCC CATTGCAAAG 
     2041 TTCTCATCAA GGTTCTGGAT GTCAATGACA ACATCCCAAG CATCCACGTC ACATGGGCCT 
     2101 CCCAGCCATC ACTGGTGTCA GAAGCTCTTC CCAAGGACAG TTTTATTGCT CTTGTCATGG 
     2161 CAGATGACTT GGATTCAGGA CACAATGGTT TGGTCCACTG CTGGCTGAGC CAAGAGCTGG 
     2221 GCCACTTCAG GCTGAAAAGA ACTAATGGCA ACACATACAT GTTGCTAACC AATGCCACAC 
     2281 TGGACAGAGA GCAGTGGCCC AAATATACCC TCACTCTGTT AGCCCAAGAC CAAGGACTCC 
     2341 AGCCCTTATC AGCCAAGAAA CAGCTCAGCA TTCAGATCAG TGACATCAAC GACAATGCAC 
     2401 CTGTGTTTGA GAAAAGCAGG TATGAAGTCT CCACGCGGGA AAACAACTTA CCCTCTCTTC 
     2461 ACCTCATTAC CATCAAGGCT CATGATGCAG ACTTGGGCAT TAATGGAAAA GTCTCATACC 
     2521 GCATCCAGGA CTCCCCAGTT GCTCACTTAG TAGCTATTGA CTCCAACACA GGAGAGGTCA 
     2581 CTGCTCAGAG GTCACTGAAC TATGAAGAGA TGGCCGGCTT TGAGTTCCAG GTGATCGCAG 
     2641 AGGACAGCGG GCAACCCATG CTTGCATCCA GTGTCTCTGT GTGGGTCAGC CTCTTGGATG 
     2701 CCAATGATAA TGCCCCAGAG GTGGTCCAGC CTGTGCTCAG CGATGGAAAA GCCAGCCTCT 
     2761 CCGTGCTTGT GAATGCCTCC ACAGGCCACC TGCTGGTGCC CATCGAGACT CCCAATGGCT 
     2821 TGGGCCCAGC GGGCACTGAC ACACCTCCAC TGGCCACTCA CAGCTCCCGG CCATTCCTTT 
     2881 TGACAACCAT TGTGGCAAGA GATGCAGACT CGGGGGCAAA TGGAGAGCCC CTCTACAGCA 
     2941 TCCGCAGTGG AAATGAAGCC CACCTCTTCA TCCTCAACCC TCATACGGGG CAGCTGTTCG 
     3001 TCAATGTCAC CAATGCCAGC AGCCTCATTG GGAGTGAGTG GGAGCTGGAG ATAGTAGTAG 
     3061 AGGACCAGGG AAGCCCCCCC TTACAGACCC GAGCCCTGTT GAGGGTCATG TTTGTCACCA 
     3121 GTGTGGACCA CCTGAGGGAC TCAGCCCGCA AGCCTGGGGC CTTGAGCATG TCGATGCTGA 
     3181 CGGTGATCTG CCTGGCTGTA CTGTTGGGCA TCTTCGGGTT GATCCTGGCT TTGTTCATGT 
     3241 CCATCTGCCG GACAGAAAAG AAGGACAACA GGGCCTACAA CTGTCGGGAG GCCGAGTCCA 
     3301 CCTACCGCCA GCAGCCCAAG AGGCCCCAGA AACACATTCA GAAGGCAGAC ATCCACCTCG 
     3361 TGCCTGTGCT CAGGGGTCAG GCAGGTGAGC CTTGTGAAGT CGGGCAGTCC CACAAAGATG 
     3421 TGGACAAGGA GGCGATGATG GAAGCAGGCT GGGACCCCTG CCTGCAGGCC CCCTTCCACC 
     3481 TCACCCCGAC CCTGTACAGG ACGCTGCGTA ATCAAGGCAA CCAGGGAGCA CCGGCGGAGA 
     3541 GCCGAGAGGT GCTGCAAGAC ACGGTCAACC TCCTTTTCAA CCATCCCAGG CAGAGGAATG 
     3601 CCTCCCGGGA GAACCTGAAC CTTCCCGAGC CCCAGCCTGC CACAGGCCAG CCACGTTCCA 
     3661 GGCCTCTGAA GGTTGCAGGC AGCCCCACAG GGAGGCTGGC TGGAGACCAG GGCAGTGAGG 
     3721 AAGCCCCACA GAGGCCACCA GCCTCCTCTG CAACCCTGAG ACGGCAGCGA CATCTCAATG 
     3781 GCAAAGTGTC CCCTGAGAAA GAATCAGGGC CCCGTCAGAT CCTGCGGAGC CTGGTCCGGC 
     3841 TGTCTGTGGC TGCCTTCGCC GAGCGGAACC CCGTGGAGGA GCTCACTGTG GATTCTCCTC 
     3901 CTGTTCAGCA AATCTCCCAG CTGCTGTCCT TGCTGCATCA GGGCCAATTC CAGCCCAAAC 
     3961 CAAACCACCG AGGAAATAAG TACTTGGCCA AGCCAGGAGG CAGCAGGAGT GCAATCCCAG 
     4021 ACACAGATGG CCCAAGTGCA AGGGCTGGAG GCCAGACAGA CCCAGAACAG GAGGAAGGGC 
     4081 CTTTGGATCC TGAAGAGGAC CTCTCTGTGA AGCAACTGCT AGAAGAAGAG CTGTCAAGTC 
     4141 TGCTGGACCC CAGCACAGGT CTGGCCCTGG ACCGGCTGAG CGCCCCTGAC CCGGCCTGGA 
     4201 TGGCGAGACT CTCTTTGCCC CTCACCACCA ACTACCGTGA CAATGTGATC TCCCCGGATG 
     4261 CTGCAGCCAC GGAGGAGCCA AGGACCTTCC AGACGTTCGG CAAGGCAGAG GCACCAGAGC 
     4321 TGAGCCCAAC AGGCACGAGG CTGGCCAGCA CCTTTGTCTC GGAGATGAGC TCACTGCTGG 
     4381 AGATGCTGCT GGAACAGCGC TCCAGCATGC CCGTGGAGGC CGCCTCCGAG GCGCTGCGGC 
     4441 GGCTCTCGGT CTGCGGGAGG ACCCTCAGTT TAGACTTGGC CACCAGTGCA GCCTCAGGCA 
     4501 TGAAAGTGCA AGGGGACCCA GGTGGAAAGA CGGGGACTGA GGGCAAGAGC AGAGGCAGCA 
     4561 GCAGCAGCAG CAGGTGCCTG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4621 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4681 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4741 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4801 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4861 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4921 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4981 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5041 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5101 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5161 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5221 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5281 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5341 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5401 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5461 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5521 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5581 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5641 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5701 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5761 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5821 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5881 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5941 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6001 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6061 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6121 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6181 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6241 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6301 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6361 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6421 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6481 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6541 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6601 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6661 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6721 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6781 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6841 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6901 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6961 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7021 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7081 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7141 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7201 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7261 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7321 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7381 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7441 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7501 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7561 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7621 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7681 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7801 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7861 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7921 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7981 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8041 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8101 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8161 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8221 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8281 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8341 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8401 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//