LOCUS       RC223002                8586 bp    DNA     circular  SYN 
DEFINITION  BRD8 (Myc-DDK-tagged)-Human bromodomain containing 8 (BRD8), transcript variant 2
ACCESSION   NM_139199
VERSION     NM_139199.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4743..4834 
                     /label="Myc-DDK"  
     misc_feature    4864..5449 
                     /label="PolyA signal"  
     misc_feature    4921..4940 
                     /label="Restriction Enzyme"  
     misc_feature    5725..6397 
                     /label="ColE1"  
     misc_feature    6827..7621 
                     /label="Kan/Neo"  
     misc_feature    7656..7958 
                     /label="SV40 ori"  
     misc_feature    8044..8521 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4725..4731 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4725 
                     /label="BRD8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACGGGA ACGGGCAAAC ACAAGCTGCT AAGCACTGGC CCCACAGAGC 
     1081 CATGGTCCAT CCGAGAGAAG CTATGTTTAG CATCTTCTGT CATGAGAAGT GGCGATCAAA 
     1141 ATTGGGTATC AGTTAGCAGA GCAATCAAGC CCTTTGCAGA ACCTGGCCGC CCTCCAGACT 
     1201 GGTTCTCTCA AAAACATTGT GCTTCCCAGT ACTCGGAGCT TTTAGAGACC ACTGAGACAC 
     1261 CAAAACGGAA ACGAGGTGAA AAGGGAGAAG TGGTGGAAAC TGTTGAAGAT GTTATTGTTC 
     1321 GGAAATTGAC TGCTGAGCGA GTTGAAGAAC TAAAGAAAGT GATAAAGGAA ACCCAGGAGA 
     1381 GATATAGACG GCTAAAGAGA GATGCAGAAC TAATTCAAGC TGGACACATG GACAGCAGAC 
     1441 TGGATGAGCT TTGCAATGAC ATTGCAACGA AAAAGAAATT GGAAGAAGAG GAGGCTGAAG 
     1501 TAAAGAGGAA GGCTACAGAT GCTGCATACC AGGCTCGTCA AGCAGTAAAA ACACCCCCCC 
     1561 GGAGGTTACC CACTGTGATG GTTCGCTCTC CTATAGATTC TGCCTCCCCA GGAGGTGATT 
     1621 ATCCACTTGG GGACTTGACT CCAACCACTA TGGAAGAGGC TACCTCTGGG GTCAATGAGA 
     1681 GTGAAATGGC TGTGGCTTCT GGCCACCTGA ACAGTACAGG TGTCCTCCTG GAGGTAGGCG 
     1741 GGGTCCTTCC CATGATACAT GGTGGGGAGA TACAGCAAAC ACCCAATACT GTTGCAGCCT 
     1801 CCCCTGCTGC ATCAGGTGCT CCCACTCTTT CCCGGCTTTT AGAAGCTGGT CCTACACAGT 
     1861 TCACCACACC TCTTGCTTCC TTCACTACTG TTGCCAGTGA GCCTCCAGTT AAACTTGTGC 
     1921 CACCCCCTGT AGAGTCTGTG TCCCAAGCTA CCATTGTCAT GATGCCTGCG CTGCCAGCAC 
     1981 CATCCTCTGC TCCGGCTGTC TCCACTACTG AAAGTGTAGC TCCAGTGAGT CAACCCGACA 
     2041 ACTGTGTTCC CATGGAGGCT GTGGGGGATC CACATACTGT GACTGTTTCC ATGGACAGCA 
     2101 GTGAAATATC CATGATCATC AATTCTATCA AAGAAGAGTG TTTTCGATCA GGGGTAGCAG 
     2161 AGGCTCCTGT TGGATCAAAG GCTCCCAGCA TAGATGGGAA GGAAGAATTA GATCTGGCTG 
     2221 AGAAGATGGA TATTGCTGTG TCTTACACAG GTGAAGAGCT GGATTTTGAG ACTGTTGGAG 
     2281 ACATCATTGC CATCATTGAG GACAAGGTAG ATGATCATCC TGAAGTGCTG GATGTGGCAG 
     2341 CAGTGGAAGC AGCACTGTCA TTTTGTGAAG AAAATGATGA TCCTCAGTCC CTGCCTGGCC 
     2401 CCTGGGAGCA TCCTATCCAG CAGGAGCGGG ACAAGCCAGT ACCTCTCCCT GCACCAGAAA 
     2461 TGACGGTCAA GCAAGAGAGA CTGGACTTTG AGGAAACGGA AAACAAGGGA ATACATGAAC 
     2521 TGGTGGACAT CAGGGAGCCC AGTGCAGAGA TCAAGGTGGA ACCTGCAGAA CCAGAGCCAG 
     2581 TCATTTCAGG AGCCGAAATA GTAGCTGGAG TTGTTCCAGC CACAAGTATG GAGCCACCAG 
     2641 AACTCAGGAG TCAGGACTTA GATGAGGAAC TGGGAAGTAC TGCAGCTGGA GAGATTGTTG 
     2701 AAGCAGATGT TGCCATTGGG AAAGGCGATG AGACTCCACT TACAAATGTG AAGACAGAGG 
     2761 CATCCCCTGA AAGCATGTTG TCTCCATCAC ATGGCTCAAA TCCCATTGAA GATCCTTTAG 
     2821 AGGCAGAGAC TCAGCACAAG TTTGAAATGT CAGACTCATT GAAAGAAGAA TCAGGGACTA 
     2881 TTTTTGGAAG CCAGATAAAG GATGCCCCAG GTGAGGATGA GGAGGAAGAT GGTGTCAGTG 
     2941 AAGCGGCCAG CCTAGAGGAG CCTAAGGAAG AGGATCAAGG AGAAGGCTAC TTGTCAGAAA 
     3001 TGGATAATGA ACCTCCTGTG AGCGAGAGTG ATGATGGCTT CAGCATACAC AATGCTACAC 
     3061 TGCAGTCACA CACACTGGCA GACTCCATCC CCAGCAGCCC TGCTTCTTCA CAGTTCTCTG 
     3121 TCTGTAGTGA GGATCAGGAA GCTATTCAGG CACAGAAAAT TTGGAAGAAA GCCATCATGC 
     3181 TTGTATGGAG AGCTGCAGCT AATCATAGGT ATGCCAATGT CTTCCTGCAG CCTGTTACAG 
     3241 ATGACATAGC ACCTGGCTAC CACAGCATTG TGCAGAGGCC TATGGATTTG TCAACTATTA 
     3301 AGAAAAACAT AGAAAATGGA CTGATCCGAA GCACAGCTGA ATTTCAGCGT GACATTATGC 
     3361 TGATGTTTCA GAATGCTGTA ATGTACAATA GCTCAGACCA TGATGTCTAT CACATGGCAG 
     3421 TGGAGATGCA GCGAGATGTC TTGGAACAGA TCCAGCAATT CTTGGCCACG CAGTTGATTA 
     3481 TGCAAACATC CGAGTCTGGG ATCAGTGCTA AAAGTCTTCG AGGGAGAGAT TCTACCCGCA 
     3541 AACAGGATGC TTCAGAGAAG GACAGTGTCC CAATGGGCTC TCCTGCCTTC CTTCTCTCTC 
     3601 TCTTTATGGG ACACGAGTGG GTTTGGCTGG ATTCTGAACA AGATCATCCC AATGACTCTG 
     3661 AGTTGAGCAA TGACTGCAGG TCCCTCTTCA GCTCATGGGA CTCCAGTCTG GATCTTGATG 
     3721 TGGGCAACTG GAGGGAAACT GAGGATCCAG AGGCTGAGGA ACTAGAGGAA AGCAGCCCGG 
     3781 AGAGAGAACC TAGTGAACTG CTTGTTGGGG ATGGAGGCAG TGAGGAATCT CAGGAAGCGG 
     3841 CAAGGAAAGC CAGCCACCAG AACCTCCTCC ACTTTCTCTC TGAGGTAGCT TATTTAATGG 
     3901 AGCCCTTGTG CATCAGCAGC AACGAATCAA GTGAAGGCTG CTGCCCTCCA TCTGGTACCA 
     3961 GACAAGAAGG AAGGGAAATT AAAGCTAGCG AAGGAGAAAG GGAGCTCTGC AGAGAGACTG 
     4021 AAGAGCTTTC AGCTAAAGGA GACCCCTTAG TAGCTGAAAA GCCACTGGGA GAAAATGGAA 
     4081 AGCCAGAGGT GGCTTCAGCT CCCTCAGTTA TTTGTACAGT TCAGGGACTA CTCACAGAGA 
     4141 GTGAAGAGGG GGAGGCTCAG CAAGAATCCA AAGGGGAGGA CCAGGGTGAA GTATATGTGT 
     4201 CAGAGATGGA AGACCAGCCC CCTTCAGGCG AGTGTGATGA TGCCTTTAAC ATTAAGGAGA 
     4261 CTCCCTTGGT GGATACACTT TTCAGCCATG CTACCTCCTC AAAGCTGACT GATCTAAGCC 
     4321 AGGATGACCC TGTTCAGGAT CATTTGCTAT TTAAGAAGAC TCTCCTGCCA GTCTGGAAGA 
     4381 TGATTGCCAG TCACAGGTTC AGCAGTCCAT TTCTGAAGCC TGTGTCAGAA AGGCAGGCCC 
     4441 CAGGGTACAA GGATGTGGTG AAAAGACCCA TGGACTTAAC TAGCCTGAAG AGAAATCTCT 
     4501 CTAAGGGTCG GATTCGCACC ATGGCCCAAT TCCTGCGAGA CCTGATGCTG ATGTTCCAAA 
     4561 ATGCTGTAAT GTACAATGAC TCTGATCATC ATGTATACCA TATGGCTGTG GAGATGCGGC 
     4621 GAGAAGTCCT GGAGCAGATT CAGGTACTGA ATATCTGGTT AGACAAAAGA AAAGGCTCAA 
     4681 GTAGTCTGGA AGGAGAACCA GCTAACCCAG TGGATGATGG AAAACCTGTT TTCACGCGTA 
     4741 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4801 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4861 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4921 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4981 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5041 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5101 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5161 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5221 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5281 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5341 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5401 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5461 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5521 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5581 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5641 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5701 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5761 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5821 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5881 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5941 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6001 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6061 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6121 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6181 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6241 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6301 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6361 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6421 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6481 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6541 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6601 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6661 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6721 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6781 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6841 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6901 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6961 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7021 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7081 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7141 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7201 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7261 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7321 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7381 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7441 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7501 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7561 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7621 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7681 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7741 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7801 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7861 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7921 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7981 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8041 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8101 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8161 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8221 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8281 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8341 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8401 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8461 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8521 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8581 AATTTT
//