LOCUS       RC222984                6864 bp    DNA     circular  SYN 
DEFINITION  JPH1 (Myc-DDK-tagged)-Human junctophilin 1 (JPH1)
ACCESSION   NM_020647
VERSION     NM_020647.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3021..3112 
                     /label="Myc-DDK"  
     misc_feature    3142..3727 
                     /label="PolyA signal"  
     misc_feature    3199..3218 
                     /label="Restriction Enzyme"  
     misc_feature    4003..4675 
                     /label="ColE1"  
     misc_feature    5105..5899 
                     /label="Kan/Neo"  
     misc_feature    5934..6236 
                     /label="SV40 ori"  
     misc_feature    6322..6799 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3003..3009 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3003 
                     /label="JPH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGGCGGA AGGTTCGACT TCGACGATGG CGGCACCTAC TGCGGCGGCT 
     1081 GGGAGGAGGG CAAGGCGCAC GGGCATGGCA TCTGCACGGG GCCCAAGGGC CAGGGCGAGT 
     1141 ACTCGGGCTC CTGGTCGCAC GGCTTCGAGG TGGTCGGAGG CTACACCTGG CCCAGCGGCA 
     1201 ACACCTACCA GGGCTACTGG GCGCAGGGCA AGCGGCACGG GCTGGGGGTG GAGACGAAGG 
     1261 GCAAGTGGAT GTACCGGGGG GAGTGGTCAC ATGGTTTCAA GGGGCGCTAC GGGGTCCGGC 
     1321 AGAGCCTGTG CACCCCCGCT CGCTACGAGG GTACCTGGAG TAACGGGCTG CAAGACGGGT 
     1381 ACGGCGTGGA GACCTACGGG GACGGAGGTA CCTACCAGGG CCAGTGGGCC GGAGGCATGC 
     1441 GGCATGGCTA CGGCGTGCGC CAGAGCGTGC CCTACGGCAT GGCCACGGTG ATCCGCTCAC 
     1501 CGCTGCGTAC CTCGCTGGCC TCGCTGCGCA GCGAGCAGAG CAATGGCAGC GTGCTCCACG 
     1561 ACGCCGCAGC CGCCGCCGAC AGCCCGGCCG GCACCCGCGG CGGTTTCGTG CTCAACTTCC 
     1621 ACGCAGACGC TGAGCTAGCG GGCAAGAAGA AGGGCGGCCT CTTCCGGAGG GGCTCCCTTC 
     1681 TTGGAAGCAT GAAACTTCGC AAGTCCGAAT CCAAGTCTTC CATCTCGAGC AAGCGCAGCT 
     1741 CTGTCCGCAG CGACGCGGCC ATGAGCAGAA TTAGTTCCAG CGATGCCAAC TCCACGATCA 
     1801 GCTTTGGCGA TGTAGATTGT GATTTTTGCC CGGTGGAAGA CCACGTGGAC GCCACCACCA 
     1861 CGGAAACCTA CATGGGCGAG TGGAAGAACG ACAAGCGCAA CGGCTTCGGC GTTAGCGAGC 
     1921 GCTCCAATGG CATGAAGTAT GAAGGGGAGT GGGCAAATAA CAAGAGGCAT GGATATGGCT 
     1981 GTACCGTGTT TCCTGACGGC TCCAAAGAAG AGGGAAAATA CAAAAATAAT ATTCTGGTCC 
     2041 GTGGGATAAG GAAGCAGCTT ATACCAATAA GACATACAAA AACTAGGGAG AAGGTGGACA 
     2101 GAGCAATTGA AGGCGCCCAA AGGGCAGCTG CCATGGCCAG AACCAAAGTG GAAATAGCAA 
     2161 ATTCAAGGAC TGCACATGCC AGAGCGAAGG CCGATGCCGC CGACCAGGCC GCGCTGGCCG 
     2221 CTCGCCAGGA GTGCGACATC GCGAGAGCTG TGGCCAGGGA GCTGTCACCT GATTTCTACC 
     2281 AACCAGGCCC TGATTACGTC AAACAGAGAT TTCAGGAAGG TGTAGATGCT AAAGAAAATC 
     2341 CAGAAGAAAA GGTACCAGAA AAGCCACCTA CACCAAAGGA GTCTCCTCAT TTTTATCGCA 
     2401 AAGGCACGAC ACCCCCAAGA TCTCCTGAGG CAAGTCCCAA ACACAGCCAC TCTCCTGCTT 
     2461 CCTCCCCAAA GCCCCTGAAG AAGCAAAACC CCAGCTCAGG GGCGAGACTC AACCAAGACA 
     2521 AAAGGAGTGT GGCTGATGAG CAGGTGACGG CCATTGTCAA TAAGCCCTTG ATGTCAAAGG 
     2581 CTCCCACGAA GGAGGCAGGA GCGGTTGTGC CCCAGTCCAA GTACTCTGGC CGCCACCACA 
     2641 TCCCCAACCC CAGTAACGGG GAGCTGCATT CTCAGTATCA CGGCTACTAC GTGAAGCTGA 
     2701 ACGCCCCCCA GCACCCTCCA GTAGACGTGG AGGACGGCGA TGGATCCAGC CAGTCTTCCT 
     2761 CAGCACTGGT GCACAAGCCA TCCGCTAACA AGTGGAGTCC CTCCAAATCT GTGACAAAAC 
     2821 CAGTTGCCAA AGAAAGCAAA GCTGAGCCAA AAGCTAAGAA GTCTGAACTT GCTATACCAA 
     2881 AGAATCCAGC AAGCAACGAT TCATGCCCTG CTTTGGAAAA AGAAGCCAAT TCAGGCCCTA 
     2941 ATTCAATCAT GATTGTCCTT GTCATGCTGT TGAATATCGG GTTGGCCATT CTTTTTGTTC 
     3001 ACTTTCTAAC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3061 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3121 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3181 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3241 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3301 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3361 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3421 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3481 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3541 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3601 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3661 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3721 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3781 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3841 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     3901 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     3961 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4021 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4081 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4141 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4201 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4261 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4321 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4381 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4441 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4501 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4561 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4621 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4681 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4741 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4801 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4861 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     4921 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     4981 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5041 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5101 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5161 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5221 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5281 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5341 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5401 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5461 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5521 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5581 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5641 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5701 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5761 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5821 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     5881 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     5941 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6001 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6061 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6121 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6181 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6241 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6301 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6361 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6421 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6481 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6541 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6601 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6661 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6721 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6781 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6841 TAACAAAAAT TTAACGCGAA TTTT
//