LOCUS       RC222909                8556 bp    DNA     circular  SYN 
DEFINITION  ERBB2 (Myc-DDK-tagged)-Human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) (ERBB2), transcript variant 2
ACCESSION   NM_001005862
VERSION     NM_001005862.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4713..4804 
                     /label="Myc-DDK"  
     misc_feature    4834..5419 
                     /label="PolyA signal"  
     misc_feature    4891..4910 
                     /label="Restriction Enzyme"  
     misc_feature    5695..6367 
                     /label="ColE1"  
     misc_feature    6797..7591 
                     /label="Kan/Neo"  
     misc_feature    7626..7928 
                     /label="SV40 ori"  
     misc_feature    8014..8491 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4695..4701 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4695 
                     /label="ERBB2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCTGCGG CTCCCTGCCA GTCCCGAGAC CCACCTGGAC ATGCTCCGCC 
     1081 ACCTCTACCA GGGCTGCCAG GTGGTGCAGG GAAACCTGGA ACTCACCTAC CTGCCCACCA 
     1141 ATGCCAGCCT GTCCTTCCTG CAGGATATCC AGGAGGTGCA GGGCTACGTG CTCATCGCTC 
     1201 ACAACCAAGT GAGGCAGGTC CCACTGCAGA GGCTGCGGAT TGTGCGAGGC ACCCAGCTCT 
     1261 TTGAGGACAA CTATGCCCTG GCCGTGCTAG ACAATGGAGA CCCGCTGAAC AATACCACCC 
     1321 CTGTCACAGG GGCCTCCCCA GGAGGCCTGC GGGAGCTGCA GCTTCGAAGC CTCACAGAGA 
     1381 TCTTGAAAGG AGGGGTCTTG ATCCAGCGGA ACCCCCAGCT CTGCTACCAG GACACGATTT 
     1441 TGTGGAAGGA CATCTTCCAC AAGAACAACC AGCTGGCTCT CACACTGATA GACACCAACC 
     1501 GCTCTCGGGC CTGCCACCCC TGTTCTCCGA TGTGTAAGGG CTCCCGCTGC TGGGGAGAGA 
     1561 GTTCTGAGGA TTGTCAGAGC CTGACGCGCA CTGTCTGTGC CGGTGGCTGT GCCCGCTGCA 
     1621 AGGGGCCACT GCCCACTGAC TGCTGCCATG AGCAGTGTGC TGCCGGCTGC ACGGGCCCCA 
     1681 AGCACTCTGA CTGCCTGGCC TGCCTCCACT TCAACCACAG TGGCATCTGT GAGCTGCACT 
     1741 GCCCAGCCCT GGTCACCTAC AACACAGACA CGTTTGAGTC CATGCCCAAT CCCGAGGGCC 
     1801 GGTATACATT CGGCGCCAGC TGTGTGACTG CCTGTCCCTA CAACTACCTT TCTACGGACG 
     1861 TGGGATCCTG CACCCTCGTC TGCCCCCTGC ACAACCAAGA GGTGACAGCA GAGGATGGAA 
     1921 CACAGCGGTG TGAGAAGTGC AGCAAGCCCT GTGCCCGAGT GTGCTATGGT CTGGGCATGG 
     1981 AGCACTTGCG AGAGGTGAGG GCAGTTACCA GTGCCAATAT CCAGGAGTTT GCTGGCTGCA 
     2041 AGAAGATCTT TGGGAGCCTG GCATTTCTGC CGGAGAGCTT TGATGGGGAC CCAGCCTCCA 
     2101 ACACTGCCCC GCTCCAGCCA GAGCAGCTCC AAGTGTTTGA GACTCTGGAA GAGATCACAG 
     2161 GTTACCTATA CATCTCAGCA TGGCCGGACA GCCTGCCTGA CCTCAGCGTC TTCCAGAACC 
     2221 TGCAAGTAAT CCGGGGACGA ATTCTGCACA ATGGCGCCTA CTCGCTGACC CTGCAAGGGC 
     2281 TGGGCATCAG CTGGCTGGGG CTGCGCTCAC TGAGGGAACT GGGCAGTGGA CTGGCCCTCA 
     2341 TCCACCATAA CACCCACCTC TGCTTCGTGC ACACGGTGCC CTGGGACCAG CTCTTTCGGA 
     2401 ACCCGCACCA AGCTCTGCTC CACACTGCCA ACCGGCCAGA GGACGAGTGT GTGGGCGAGG 
     2461 GCCTGGCCTG CCACCAGCTG TGCGCCCGAG GGCACTGCTG GGGTCCAGGG CCCACCCAGT 
     2521 GTGTCAACTG CAGCCAGTTC CTTCGGGGCC AGGAGTGCGT GGAGGAATGC CGAGTACTGC 
     2581 AGGGGCTCCC CAGGGAGTAT GTGAATGCCA GGCACTGTTT GCCGTGCCAC CCTGAGTGTC 
     2641 AGCCCCAGAA TGGCTCAGTG ACCTGTTTTG GACCGGAGGC TGACCAGTGT GTGGCCTGTG 
     2701 CCCACTATAA GGACCCTCCC TTCTGCGTGG CCCGCTGCCC CAGCGGTGTG AAACCTGACC 
     2761 TCTCCTACAT GCCCATCTGG AAGTTTCCAG ATGAGGAGGG CGCATGCCAG CCTTGCCCCA 
     2821 TCAACTGCAC CCACTCCTGT GTGGACCTGG ATGACAAGGG CTGCCCCGCC GAGCAGAGAG 
     2881 CCAGCCCTCT GACGTCCATC ATCTCTGCGG TGGTTGGCAT TCTGCTGGTC GTGGTCTTGG 
     2941 GGGTGGTCTT TGGGATCCTC ATCAAGCGAC GGCAGCAGAA GATCCGGAAG TACACGATGC 
     3001 GGAGACTGCT GCAGGAAACG GAGCTGGTGG AGCCGCTGAC ACCTAGCGGA GCGATGCCCA 
     3061 ACCAGGCGCA GATGCGGATC CTGAAAGAGA CGGAGCTGAG GAAGGTGAAG GTGCTTGGAT 
     3121 CTGGCGCTTT TGGCACAGTC TACAAGGGCA TCTGGATCCC TGATGGGGAG AATGTGAAAA 
     3181 TTCCAGTGGC CATCAAAGTG TTGAGGGAAA ACACATCCCC CAAAGCCAAC AAAGAAATCT 
     3241 TAGACGAAGC ATACGTGATG GCTGGTGTGG GCTCCCCATA TGTCTCCCGC CTTCTGGGCA 
     3301 TCTGCCTGAC ATCCACGGTG CAGCTGGTGA CACAGCTTAT GCCCTATGGC TGCCTCTTAG 
     3361 ACCATGTCCG GGAAAACCGC GGACGCCTGG GCTCCCAGGA CCTGCTGAAC TGGTGTATGC 
     3421 AGATTGCCAA GGGGATGAGC TACCTGGAGG ATGTGCGGCT CGTACACAGG GACTTGGCCG 
     3481 CTCGGAACGT GCTGGTCAAG AGTCCCAACC ATGTCAAAAT TACAGACTTC GGGCTGGCTC 
     3541 GGCTGCTGGA CATTGACGAG ACAGAGTACC ATGCAGATGG GGGCAAGGTG CCCATCAAGT 
     3601 GGATGGCGCT GGAGTCCATT CTCCGCCGGC GGTTCACCCA CCAGAGTGAT GTGTGGAGTT 
     3661 ATGGTGTGAC TGTGTGGGAG CTGATGACTT TTGGGGCCAA ACCTTACGAT GGGATCCCAG 
     3721 CCCGGGAGAT CCCTGACCTG CTGGAAAAGG GGGAGCGGCT GCCCCAGCCC CCCATCTGCA 
     3781 CCATTGATGT CTACATGATC ATGGTCAAAT GTTGGATGAT TGACTCTGAA TGTCGGCCAA 
     3841 GATTCCGGGA GTTGGTGTCT GAATTCTCCC GCATGGCCAG GGACCCCCAG CGCTTTGTGG 
     3901 TCATCCAGAA TGAGGACTTG GGCCCAGCCA GTCCCTTGGA CAGCACCTTC TACCGCTCAC 
     3961 TGCTGGAGGA CGATGACATG GGGGACCTGG TGGATGCTGA GGAGTATCTG GTACCCCAGC 
     4021 AGGGCTTCTT CTGTCCAGAC CCTGCCCCGG GCGCTGGGGG CATGGTCCAC CACAGGCACC 
     4081 GCAGCTCATC TACCAGGAGT GGCGGTGGGG ACCTGACACT AGGGCTGGAG CCCTCTGAAG 
     4141 AGGAGGCCCC CAGGTCTCCA CTGGCACCCT CCGAAGGGGC TGGCTCCGAT GTATTTGATG 
     4201 GTGACCTGGG AATGGGGGCA GCCAAGGGGC TGCAAAGCCT CCCCACACAT GACCCCAGCC 
     4261 CTCTACAGCG GTACAGTGAG GACCCCACAG TACCCCTGCC CTCTGAGACT GATGGCTACG 
     4321 TTGCCCCCCT GACCTGCAGC CCCCAGCCTG AATATGTGAA CCAGCCAGAT GTTCGGCCCC 
     4381 AGCCCCCTTC GCCCCGAGAG GGCCCTCTGC CTGCTGCCCG ACCTGCTGGT GCCACTCTGG 
     4441 AAAGGCCCAA GACTCTCTCC CCAGGGAAGA ATGGGGTCGT CAAAGACGTT TTTGCCTTTG 
     4501 GGGGTGCCGT GGAGAACCCC GAGTACTTGA CACCCCAGGG AGGAGCTGCC CCTCAGCCCC 
     4561 ACCCTCCTCC TGCCTTCAGC CCAGCCTTCG ACAACCTCTA TTACTGGGAC CAGGACCCAC 
     4621 CAGAGCGGGG GGCTCCACCC AGCACCTTCA AAGGGACACC TACGGCAGAG AACCCAGAGT 
     4681 ACCTGGGTCT GGACGTGCCA GTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4741 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4801 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4861 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4921 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4981 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5041 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5101 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5161 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5221 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5281 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5341 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5401 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5461 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5521 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5581 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5641 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5701 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5761 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5821 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5881 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5941 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6001 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6061 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6121 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6181 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6241 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6301 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6361 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6421 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6481 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6541 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6601 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6661 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6721 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6781 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6841 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6901 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6961 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7021 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7081 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7141 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7201 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7261 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7321 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7381 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7441 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7501 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7561 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7621 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7681 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7741 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7801 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7861 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7921 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7981 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8041 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8101 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8161 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8221 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8281 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8341 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8401 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8461 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8521 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//