LOCUS       RC222906                8673 bp    DNA     circular  SYN 
DEFINITION  ADCY5 (Myc-DDK-tagged)-Human adenylate cyclase 5 (ADCY5), transcript variant 1
ACCESSION   NM_183357
VERSION     NM_183357.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4828..4919 
                     /label="Myc-DDK"  
     misc_feature    4949..5534 
                     /label="PolyA signal"  
     misc_feature    5006..5025 
                     /label="Restriction Enzyme"  
     misc_feature    5810..6482 
                     /label="ColE1"  
     misc_feature    6912..7706 
                     /label="Kan/Neo"  
     misc_feature    7741..8043 
                     /label="SV40 ori"  
     misc_feature    8129..8606 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4803..4810 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4803 
                     /label="ADCY5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCGGCTCC AAAAGCGTGA GCCCCCCGGG CTACGCGGCG CAGAAGACTG 
     1081 CGGCGCCGGC GCCCCGGGGA GGCCCCGAAC ACCGCTCTGC GTGGGGCGAG GCCGATTCCC 
     1141 GCGCGAATGG CTACCCCCAT GCCCCCGGGG GCTCTGCCCG CGGCTCCACC AAGAAACCCG 
     1201 GGGGGGCGGT GACCCCGCAG CAGCAGCAGC GCCTGGCCAG CCGCTGGCGC AGCGACGACG 
     1261 ACGACGATCC TCCGCTGAGC GGTGACGACC CCCTGGCCGG GGGCTTCGGC TTCAGCTTCC 
     1321 GCTCCAAGTC CGCCTGGCAG GAGCGCGGCG GCGACGACTG CGGTCGCGGC AGCCGCCGGC 
     1381 AGCGGCGGGG CGCGGCCAGC GGGGGCAGCA CCCGGGCGCC CCCTGCGGGC GGCGGCGGCG 
     1441 GCTCGGCGGC GGCGGCTGCC TCGGCGGGCG GGACGGAGGT GCGCCCTCGC TCGGTGGAGG 
     1501 TGGGTCTGGA GGAGCGGCGG GGCAAGGGGC GCGCGGCCGA CGAGCTGGAG GCCGGCGCCG 
     1561 TCGAGGGCGG CGAGGGGTCC GGGGATGGCG GCAGCTCGGC GGACTCGGGC TCGGGCGCGG 
     1621 GGCCCGGCGC GGTGCTGTCC CTGGGCGCCT GCTGCCTGGC GTTGCTGCAG ATATTCCGCT 
     1681 CCAAGAAGTT CCCGTCGGAC AAACTGGAGC GGCTGTACCA GCGCTACTTC TTCCGCCTGA 
     1741 ACCAGAGCAG CCTCACCATG CTCATGGCCG TGCTGGTGCT CGTGTGCCTG GTCATGTTGG 
     1801 CCTTCCACGC GGCGCGGCCC CCGCTCCAGC TGCCCTACCT GGCCGTGCTG GCGGCCGCCG 
     1861 TCGGCGTGAT CCTCATCATG GCTGTGCTTT GCAACCGCGC CGCCTTCCAC CAGGACCACA 
     1921 TGGGCCTGGC CTGCTATGCG CTCATCGCCG TGGTGCTGGC CGTCCAGGTG GTGGGCCTGC 
     1981 TGCTGCCGCA GCCACGCAGC GCCTCTGAGG GCATCTGGTG GACCGTGTTC TTCATCTACA 
     2041 CCATCTACAC GCTGCTGCCC GTGCGCATGC GGGCCGCAGT GCTCAGCGGG GTGCTCCTGT 
     2101 CCGCCCTCCA CCTGGCCATC GCCCTGCGCA CCAACGCCCA GGACCAGTTC CTGCTGAAGC 
     2161 AGCTTGTCTC CAATGTTCTC ATTTTCTCCT GCACCAACAT CGTGGGTGTC TGCACCCACT 
     2221 ATCCGGCTGA GGTCTCCCAG AGACAGGCTT TCCAGGAGAC CCGAGAGTGC ATCCAGGCGC 
     2281 GGCTCCACTC GCAGCGGGAG AACCAGCAGC AGGAACGGCT CCTGCTGTCT GTCCTTCCCC 
     2341 GTCATGTTGC CATGGAGATG AAAGCAGACA TCAACGCCAA GCAGGAGGAT ATGATGTTCC 
     2401 ATAAGATTTA CATCCAGAAA CATGACAACG TGAGCATCCT GTTTGCTGAC ATCGAGGGCT 
     2461 TCACCAGCCT GGCGTCCCAG TGCACTGCAC AGGAACTGGT CATGACCCTC AACGAGCTCT 
     2521 TCGCCCGCTT TGACAAGCTG GCCGCAGAGA ATCACTGTTT ACGTATTAAG ATCCTTGGGG 
     2581 ATTGTTATTA CTGCGTCTCG GGGCTGCCTG AAGCAAGGGC TGACCACGCC CACTGCTGTG 
     2641 TGGAGATGGG CATGGACATG ATCGAGGCCA TCTCGTTGGT CCGGGAGGTG ACAGGGGTGA 
     2701 ACGTGAACAT GCGTGTGGGA ATTCACAGCG GGCGAGTACA CTGCGGTGTC CTTGGTCTCA 
     2761 GGAAGTGGCA GTTCGACGTC TGGTCTAACG ATGTCACGCT AGCCAACCAC ATGGAGGCTG 
     2821 GCGGCAAGGC AGGACGCATC CACATCACCA AGGCTACACT CAACTACCTG AATGGGGACT 
     2881 ACGAGGTGGA GCCAGGCTGT GGGGGCGAGC GCAACGCCTA CCTCAAGGAG CACAGTATCG 
     2941 AGACCTTCCT CATCCTGCGC TGCACCCAGA AGCGGAAAGA AGAGAAGGCC ATGATCGCCA 
     3001 AGATGAACCG CCAGAGAACC AACTCCATCG GGCACAACCC ACCACACTGG GGGGCTGAGC 
     3061 GCCCCTTCTA CAACCACCTG GGTGGCAACC AGGTGTCCAA GGAGATGAAG CGGATGGGCT 
     3121 TTGAAGACCC CAAGGACAAG AACGCCCAGG AGAGTGCGAA CCCTGAGGAT GAAGTGGATG 
     3181 AGTTTCTGGG CCGTGCCATT GACGCCAGGA GCATTGATAG GCTTCGGTCT GAGCACGTCC 
     3241 GCAAGTTCCT CCTGACCTTC AGGGAGCCTG ACTTAGAGAA GAAGTACTCC AAGCAGGTAG 
     3301 ACGACCGATT TGGTGCCTAT GTGGCGTGTG CCTCGCTCGT CTTCCTCTTC ATCTGCTTTG 
     3361 TCCAGATCAC CATCGTGCCC CACTCCATAT TCATGCTCAG CTTCTACCTG ACCTGTTCCC 
     3421 TGCTGCTGAC CTTGGTGGTG TTTGTGTCTG TGATCTACTC CTGCGTAAAG CTCTTCCCCT 
     3481 CCCCACTGCA GACCCTCTCC AGGAAGATCG TGCGGTCCAA GATGAACAGC ACCCTGGTTG 
     3541 GGGTGTTCAC CATCACCCTG GTGTTCCTGG CGGCTTTTGT CAACATGTTC ACGTGCAACT 
     3601 CCAGGGACCT GCTGGGCTGC TTGGCACAGG AGCACAACAT CAGCGCGAGC CAGGTCAACG 
     3661 CGTGTCACGT GGCGGAGTCG GCCGTCAACT ACAGCCTGGG CGATGAGCAG GGCTTCTGTG 
     3721 GCAGCCCCTG GCCCAACTGC AACTTCCCCG AGTACTTCAC CTACAGCGTG CTGCTCAGCC 
     3781 TGCTGGCCTG CTCCGTGTTC CTGCAGATCA GCTGCATCGG GAAGCTGGTG CTCATGCTGG 
     3841 CCATCGAGCT CATCTACGTG CTCATCGTGG AGGTGCCAGG TGTCACGCTC TTCGACAACG 
     3901 CCGACCTGCT GGTCACCGCC AACGCCATAG ACTTCTTCAA CAACGGGACC TCCCAGTGCC 
     3961 CTGAGCATGC AACCAAGGTG GCATTGAAGG TGGTGACGCC CATCATCATC TCAGTCTTTG 
     4021 TGCTGGCCCT GTACCTGCAC GCCCAGCAGG TGGAGTCCAC TGCCCGCCTC GACTTCCTCT 
     4081 GGAAACTGCA GGCCACAGAG GAGAAAGAGG AGATGGAGGA GCTGCAGGCC TACAACCGGC 
     4141 GGCTGCTGCA CAACATCCTG CCCAAGGACG TGGCCGCTCA CTTCCTGGCC CGCGAGCGGC 
     4201 GCAATGATGA GCTCTACTAT CAGTCCTGTG AGTGTGTGGC GGTCATGTTC GCCTCCATCG 
     4261 CCAACTTCTC CGAGTTCTAC GTTGAGCTGG AGGCCAACAA CGAGGGTGTC GAGTGCCTGC 
     4321 GGCTACTCAA TGAGATCATC GCTGACTTTG ATGAGATCAT CAGCGAGGAT CGGTTCCGGC 
     4381 AGCTGGAGAA GATCAAGACC ATCGGCAGCA CCTACATGGC TGCCTCCGGC CTCAACGACT 
     4441 CTACCTACGA CAAGGTGGGC AAGACCCACA TCAAGGCACT GGCCGACTTT GCCATGAAGC 
     4501 TGATGGACCA GATGAAGTAC ATCAATGAGC ACTCCTTCAA CAACTTCCAG ATGAAGATCG 
     4561 GGCTCAACAT CGGCCCCGTG GTGGCCGGGG TGATAGGGGC ACGAAAGCCT CAGTACGACA 
     4621 TCTGGGGCAA TACCGTGAAC GTGGCCAGCC GCATGGACAG CACCGGTGTA CCCGACCGCA 
     4681 TCCAGGTCAC CACAGACATG TACCAGGTGC TGGCTGCCAA CACGTACCAG CTGGAGTGCC 
     4741 GGGGCGTGGT CAAGGTCAAG GGCAAAGGCG AGATGATGAC CTACTTCCTC AATGGAGGGC 
     4801 CCCCGCTCAG TAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4861 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4921 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4981 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5041 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5101 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5161 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5221 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5281 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5341 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5401 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5461 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5521 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5581 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5641 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5701 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5761 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5821 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5881 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5941 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6001 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6061 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6121 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6181 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6241 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6301 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6361 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6421 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6481 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6541 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6601 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6661 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6721 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6781 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6841 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6901 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6961 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7021 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7081 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7141 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7201 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7261 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7321 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7381 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7441 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7501 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7561 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7621 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7681 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7741 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7801 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7861 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7921 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7981 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8041 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8101 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8161 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8221 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8281 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8341 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8401 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8461 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8521 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8581 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8641 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//