LOCUS       RC222862L2                9480 bp    DNA     circular  SYN 
DEFINITION  ATXN1 (Myc-DDK-tagged)-Human ataxin 1 (ATXN1), transcript variant 1
ACCESSION   NM_000332
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7771..7951 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8679..9338 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7999..8618 
                     /label="ColE1"  
     misc_feature    6646..6666 
                     /label="LR50"  
     misc_feature    5786..6511 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6655..7246 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5765..5771 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5765 
                     /label="ATXN1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAAATCCAAC CAAGAGCGGA GCAACGAATG 
     3361 CCTGCCTCCC AAGAAGCGCG AGATCCCCGC CACCAGCCGG TCCTCCGAGG AGAAGGCCCC 
     3421 TACCCTGCCC AGCGACAACC ACCGGGTGGA GGGCACAGCA TGGCTCCCGG GCAACCCTGG 
     3481 TGGCCGGGGC CACGGGGGCG GGAGGCATGG GCCGGCAGGG ACCTCGGTGG AGCTTGGTTT 
     3541 ACAACAGGGA ATAGGTTTAC ACAAAGCATT GTCCACAGGG CTGGACTACT CCCCGCCCAG 
     3601 CGCTCCCAGG TCTGTCCCCG TGGCCACCAC GCTGCCTGCC GCGTACGCCA CCCCGCAGCC 
     3661 AGGGACCCCG GTGTCCCCCG TGCAGTACGC TCACCTGCCG CACACCTTCC AGTTCATTGG 
     3721 GTCCTCCCAA TACAGTGGAA CCTATGCCAG CTTCATCCCA TCACAGCTGA TCCCCCCAAC 
     3781 CGCCAACCCC GTCACCAGTG CAGTGGCCTC GGCCGCAGGG GCCACCACTC CATCCCAGCG 
     3841 CTCCCAGCTG GAGGCCTATT CCACTCTGCT GGCCAACATG GGCAGTCTGA GCCAGACGCC 
     3901 GGGACACAAG GCTGAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCATCAGCA 
     3961 TCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCACCTCA GCAGGGCTCC 
     4021 GGGGCTCATC ACCCCGGGGT CCCCCCCACC AGCCCAGCAG AACCAGTACG TCCACATTTC 
     4081 CAGTTCTCCG CAGAACACCG GCCGCACCGC CTCTCCTCCG GCCATCCCCG TCCACCTCCA 
     4141 CCCCCACCAG ACGATGATCC CACACACGCT CACCCTGGGG CCCCCCTCCC AGGTCGTCAT 
     4201 GCAATACGCC GACTCCGGCA GCCACTTTGT CCCTCGGGAG GCCACCAAGA AAGCTGAGAG 
     4261 CAGCCGGCTG CAGCAGGCCA TCCAGGCCAA GGAGGTCCTG AACGGTGAGA TGGAGAAGAG 
     4321 CCGGCGGTAC GGGGCCCCGT CCTCAGCCGA CCTGGGCCTG GGCAAGGCAG GCGGCAAGTC 
     4381 GGTTCCTCAC CCGTACGAGT CCAGGCACGT GGTGGTCCAC CCGAGCCCCT CAGACTACAG 
     4441 CAGTCGTGAT CCTTCGGGGG TCCGGGCCTC TGTGATGGTC CTGCCCAACA GCAACACGCC 
     4501 CGCAGCTGAC CTGGAGGTGC AACAGGCCAC TCATCGTGAA GCCTCCCCTT CTACCCTCAA 
     4561 CGACAAAAGT GGCCTGCATT TAGGGAAGCC TGGCCACCGG TCCTACGCGC TCTCACCCCA 
     4621 CACGGTCATT CAGACCACAC ACAGTGCTTC AGAGCCACTC CCGGTGGGAC TGCCAGCCAC 
     4681 GGCCTTCTAC GCAGGGACTC AACCCCCTGT CATCGGCTAC CTGAGCGGCC AGCAGCAAGC 
     4741 AATCACCTAC GCCGGCAGCC TGCCCCAGCA CCTGGTGATC CCCGGCACAC AGCCCCTGCT 
     4801 CATCCCGGTC GGCAGCACTG ACATGGAAGC GTCGGGGGCA GCCCCGGCCA TAGTCACGTC 
     4861 ATCCCCCCAG TTTGCTGCAG TGCCTCACAC GTTCGTCACC ACCGCCCTTC CCAAGAGCGA 
     4921 GAACTTCAAC CCTGAGGCCC TGGTCACCCA GGCCGCCTAC CCAGCCATGG TGCAGGCCCA 
     4981 GATCCACCTG CCTGTGGTGC AGTCCGTGGC CTCCCCGGCG GCGGCTCCCC CTACGCTGCC 
     5041 TCCCTACTTC ATGAAAGGCT CCATCATCCA GTTGGCCAAC GGGGAGCTAA AGAAGGTGGA 
     5101 AGACTTAAAA ACAGAAGATT TCATCCAGAG TGCAGAGATA AGCAACGACC TGAAGATCGA 
     5161 CTCCAGCACC GTAGAGAGGA TTGAAGACAG CCATAGCCCG GGCGTGGCCG TGATACAGTT 
     5221 CGCCGTCGGG GAGCACCGAG CCCAGGTCAG CGTTGAAGTT TTGGTAGAGT ATCCTTTTTT 
     5281 TGTGTTTGGA CAGGGCTGGT CATCCTGCTG TCCGGAGAGA ACCAGCCAGC TCTTTGATTT 
     5341 GCCGTGTTCC AAACTCTCAG TTGGGGATGT CTGCATCTCG CTTACCCTCA AGAACCTGAA 
     5401 GAACGGCTCT GTTAAAAAGG GCCAGCCCGT GGATCCCGCC AGCGTCCTGC TGAAGCACTC 
     5461 AAAGGCCGAC GGCCTGGCGG GCAGCAGACA CAGGTATGCC GAGCAGGAAA ACGGAATCAA 
     5521 CCAGGGGAGT GCCCAGATGC TCTCTGAGAA TGGCGAACTG AAGTTTCCAG AGAAAATGGG 
     5581 ATTGCCTGCA GCGCCCTTCC TCACCAAAAT AGAACCCAGC AAGCCCGCGG CAACGAGGAA 
     5641 GAGGAGGTGG TCGGCGCCAG AGAGCCGCAA ACTGGAGAAG TCAGAAGACG AACCACCTTT 
     5701 GACTCTTCCT AAGCCTTCTC TAATTCCTCA GGAGGTTAAG ATTTGCATTG AAGGCCGGTC 
     5761 TAATGTAGGC AAGACGCGTA CGCGGCCGCT CGAGATGAGC GGGGGCGAGG AGCTGTTCGC 
     5821 CGGCATCGTG CCCGTGCTGA TCGAGCTGGA CGGCGACGTG CACGGCCACA AGTTCAGCGT 
     5881 GCGCGGCGAG GGCGAGGGCG ACGCCGACTA CGGCAAGCTG GAGATCAAGT TCATCTGCAC 
     5941 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTGGTGACC ACCCTCTGCT ACGGCATCCA 
     6001 GTGCTTCGCC CGCTACCCCG AGCACATGAA GATGAACGAC TTCTTCAAGA GCGCCATGCC 
     6061 CGAGGGCTAC ATCCAGGAGC GCACCATCCA GTTCCAGGAC GACGGCAAGT ACAAGACCCG 
     6121 CGGCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCAAGGA 
     6181 CTTCAAGGAG GACGGCAACA TCCTGGGCCA CAAGCTGGAG TACAGCTTCA ACAGCCACAA 
     6241 CGTGTACATC CGCCCCGACA AGGCCAACAA CGGCCTGGAG GCTAACTTCA AGACCCGCCA 
     6301 CAACATCGAG GGCGGCGGCG TGCAGCTGGC CGACCACTAC CAGACCAACG TGCCCCTGGG 
     6361 CGACGGCCCC GTGCTGATCC CCATCAACCA CTACCTGAGC ACTCAGACCA AGATCAGCAA 
     6421 GGACCGCAAC GAGGCCCGCG ACCACATGGT GCTCCTGGAG TCCTTCAGCG CCTGCTGCCA 
     6481 CACCCACGGC ATGGACGAGC TGTACAGGTC CGGACTCAGA TAAGTTTAAA CGGCCGGCCG 
     6541 CGGTCTGTAC AAGTAGGATT CGTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC 
     6601 GAAGTTATAC ATGTTTAAGG GTTCCGGTTC CACTAGGTAC AATTCGATAT CAAGCTTATC 
     6661 GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT 
     6721 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC 
     6781 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG 
     6841 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC 
     6901 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC 
     6961 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG 
     7021 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG 
     7081 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC 
     7141 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT 
     7201 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC 
     7261 CGTCGACCTC GATCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC 
     7321 TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT 
     7381 CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT 
     7441 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT 
     7501 TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG 
     7561 GCCAGGGATC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA 
     7621 AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT 
     7681 GCATGGGATG GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA GCCGCCTAGC 
     7741 ATTTCATCAC ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT GGTTAGACCA 
     7801 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG 
     7861 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG 
     7921 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGCATGTGAG CAAAAGGCCA 
     7981 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     8041 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     8101 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     8161 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     8221 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     8281 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     8341 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     8401 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     8461 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     8521 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     8581 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     8641 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGATTA CGCCCCGCCC 
     8701 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA 
     8761 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA 
     8821 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA 
     8881 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT 
     8941 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA 
     9001 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA 
     9061 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT 
     9121 GCCATACGGA ACTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA 
     9181 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 
     9241 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC 
     9301 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATACT CTTCCTTTTT 
     9361 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     9421 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//