LOCUS       RC222862                7326 bp    DNA     circular  SYN 
DEFINITION  ATXN1 (Myc-DDK-tagged)-Human ataxin 1 (ATXN1), transcript variant 1
ACCESSION   NM_000332
VERSION     NM_000332.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3483..3574 
                     /label="Myc-DDK"  
     misc_feature    3604..4189 
                     /label="PolyA signal"  
     misc_feature    3661..3680 
                     /label="Restriction Enzyme"  
     misc_feature    4465..5137 
                     /label="ColE1"  
     misc_feature    5567..6361 
                     /label="Kan/Neo"  
     misc_feature    6396..6698 
                     /label="SV40 ori"  
     misc_feature    6784..7261 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3465..3471 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3465 
                     /label="ATXN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAATCCAAC CAAGAGCGGA GCAACGAATG CCTGCCTCCC AAGAAGCGCG 
     1081 AGATCCCCGC CACCAGCCGG TCCTCCGAGG AGAAGGCCCC TACCCTGCCC AGCGACAACC 
     1141 ACCGGGTGGA GGGCACAGCA TGGCTCCCGG GCAACCCTGG TGGCCGGGGC CACGGGGGCG 
     1201 GGAGGCATGG GCCGGCAGGG ACCTCGGTGG AGCTTGGTTT ACAACAGGGA ATAGGTTTAC 
     1261 ACAAAGCATT GTCCACAGGG CTGGACTACT CCCCGCCCAG CGCTCCCAGG TCTGTCCCCG 
     1321 TGGCCACCAC GCTGCCTGCC GCGTACGCCA CCCCGCAGCC AGGGACCCCG GTGTCCCCCG 
     1381 TGCAGTACGC TCACCTGCCG CACACCTTCC AGTTCATTGG GTCCTCCCAA TACAGTGGAA 
     1441 CCTATGCCAG CTTCATCCCA TCACAGCTGA TCCCCCCAAC CGCCAACCCC GTCACCAGTG 
     1501 CAGTGGCCTC GGCCGCAGGG GCCACCACTC CATCCCAGCG CTCCCAGCTG GAGGCCTATT 
     1561 CCACTCTGCT GGCCAACATG GGCAGTCTGA GCCAGACGCC GGGACACAAG GCTGAGCAGC 
     1621 AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCATCAGCA TCAGCAGCAG CAGCAGCAGC 
     1681 AGCAGCAGCA GCAGCAGCAG CAGCACCTCA GCAGGGCTCC GGGGCTCATC ACCCCGGGGT 
     1741 CCCCCCCACC AGCCCAGCAG AACCAGTACG TCCACATTTC CAGTTCTCCG CAGAACACCG 
     1801 GCCGCACCGC CTCTCCTCCG GCCATCCCCG TCCACCTCCA CCCCCACCAG ACGATGATCC 
     1861 CACACACGCT CACCCTGGGG CCCCCCTCCC AGGTCGTCAT GCAATACGCC GACTCCGGCA 
     1921 GCCACTTTGT CCCTCGGGAG GCCACCAAGA AAGCTGAGAG CAGCCGGCTG CAGCAGGCCA 
     1981 TCCAGGCCAA GGAGGTCCTG AACGGTGAGA TGGAGAAGAG CCGGCGGTAC GGGGCCCCGT 
     2041 CCTCAGCCGA CCTGGGCCTG GGCAAGGCAG GCGGCAAGTC GGTTCCTCAC CCGTACGAGT 
     2101 CCAGGCACGT GGTGGTCCAC CCGAGCCCCT CAGACTACAG CAGTCGTGAT CCTTCGGGGG 
     2161 TCCGGGCCTC TGTGATGGTC CTGCCCAACA GCAACACGCC CGCAGCTGAC CTGGAGGTGC 
     2221 AACAGGCCAC TCATCGTGAA GCCTCCCCTT CTACCCTCAA CGACAAAAGT GGCCTGCATT 
     2281 TAGGGAAGCC TGGCCACCGG TCCTACGCGC TCTCACCCCA CACGGTCATT CAGACCACAC 
     2341 ACAGTGCTTC AGAGCCACTC CCGGTGGGAC TGCCAGCCAC GGCCTTCTAC GCAGGGACTC 
     2401 AACCCCCTGT CATCGGCTAC CTGAGCGGCC AGCAGCAAGC AATCACCTAC GCCGGCAGCC 
     2461 TGCCCCAGCA CCTGGTGATC CCCGGCACAC AGCCCCTGCT CATCCCGGTC GGCAGCACTG 
     2521 ACATGGAAGC GTCGGGGGCA GCCCCGGCCA TAGTCACGTC ATCCCCCCAG TTTGCTGCAG 
     2581 TGCCTCACAC GTTCGTCACC ACCGCCCTTC CCAAGAGCGA GAACTTCAAC CCTGAGGCCC 
     2641 TGGTCACCCA GGCCGCCTAC CCAGCCATGG TGCAGGCCCA GATCCACCTG CCTGTGGTGC 
     2701 AGTCCGTGGC CTCCCCGGCG GCGGCTCCCC CTACGCTGCC TCCCTACTTC ATGAAAGGCT 
     2761 CCATCATCCA GTTGGCCAAC GGGGAGCTAA AGAAGGTGGA AGACTTAAAA ACAGAAGATT 
     2821 TCATCCAGAG TGCAGAGATA AGCAACGACC TGAAGATCGA CTCCAGCACC GTAGAGAGGA 
     2881 TTGAAGACAG CCATAGCCCG GGCGTGGCCG TGATACAGTT CGCCGTCGGG GAGCACCGAG 
     2941 CCCAGGTCAG CGTTGAAGTT TTGGTAGAGT ATCCTTTTTT TGTGTTTGGA CAGGGCTGGT 
     3001 CATCCTGCTG TCCGGAGAGA ACCAGCCAGC TCTTTGATTT GCCGTGTTCC AAACTCTCAG 
     3061 TTGGGGATGT CTGCATCTCG CTTACCCTCA AGAACCTGAA GAACGGCTCT GTTAAAAAGG 
     3121 GCCAGCCCGT GGATCCCGCC AGCGTCCTGC TGAAGCACTC AAAGGCCGAC GGCCTGGCGG 
     3181 GCAGCAGACA CAGGTATGCC GAGCAGGAAA ACGGAATCAA CCAGGGGAGT GCCCAGATGC 
     3241 TCTCTGAGAA TGGCGAACTG AAGTTTCCAG AGAAAATGGG ATTGCCTGCA GCGCCCTTCC 
     3301 TCACCAAAAT AGAACCCAGC AAGCCCGCGG CAACGAGGAA GAGGAGGTGG TCGGCGCCAG 
     3361 AGAGCCGCAA ACTGGAGAAG TCAGAAGACG AACCACCTTT GACTCTTCCT AAGCCTTCTC 
     3421 TAATTCCTCA GGAGGTTAAG ATTTGCATTG AAGGCCGGTC TAATGTAGGC AAGACGCGTA 
     3481 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3541 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3601 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3661 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3721 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3781 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3841 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3901 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3961 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4021 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4081 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4141 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4201 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4261 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4321 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4381 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4441 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4501 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4561 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4621 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4681 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4741 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4801 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4861 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4921 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4981 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5041 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5101 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5161 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5221 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5281 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5341 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5401 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5461 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5521 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5581 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5641 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5701 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5761 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5821 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5881 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5941 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6001 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6061 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6121 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6181 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6241 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6301 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6361 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6421 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6481 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6541 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6601 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6661 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6721 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6781 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6841 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6901 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6961 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7021 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7081 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7141 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7201 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7261 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7321 AATTTT
//