LOCUS       RC222738                8238 bp    DNA     circular  SYN 
DEFINITION  ADCY1 (Myc-DDK-tagged)-Human adenylate cyclase 1 (brain) (ADCY1)
ACCESSION   NM_021116
VERSION     NM_021116.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4395..4486 
                     /label="Myc-DDK"  
     misc_feature    4516..5101 
                     /label="PolyA signal"  
     misc_feature    4573..4592 
                     /label="Restriction Enzyme"  
     misc_feature    5377..6049 
                     /label="ColE1"  
     misc_feature    6479..7273 
                     /label="Kan/Neo"  
     misc_feature    7308..7610 
                     /label="SV40 ori"  
     misc_feature    7696..8173 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4377..4383 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4377 
                     /label="ADCY1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGGGCG CCGCGCGGCG GAGGCGGCGG CGGAGGCGGC GCGGGCGAGC 
     1081 CCGGGGGCGC CGAGCGGGCG GCCGGGACAA GCCGCCGGCG CGGGCTCCGG GCGTGCGACG 
     1141 AGGAGTTCGC TTGCCCAGAG CTGGAGGCGC TGTTCCGCGG CTACACGCTG CGGCTGGAGC 
     1201 AGGCGGCCAC GCTGAAGGCG CTGGCCGTTC TCAGCCTGCT GGCGGGCGCG CTGGCGCTGG 
     1261 CCGAGCTGCT GGGCGCGCCG GGGCCCGCGC CCGGCCTGGC CAAGGGCTCA CACCCGGTGC 
     1321 ACTGCGTCCT CTTCCTGGCG CTGCTCGTGG TAACCAACGT CCGGTCCCTG CAGGTGCCCC 
     1381 AGCTGCAGCA GGTCGGCCAG CTGGCGCTGC TCTTCAGCCT CACCTTCGCG CTGCTCTGCT 
     1441 GTCCTTTCGC GCTGGGCGGC CCCGCCCGGG GTTCCGCCGG GGCCGCTGGG GGGCCAGCGA 
     1501 CCGCCGAACA AGGGGTTTGG CAGCTCCTTT TGGTCACCTT CGTGTCCTAT GCCTTGCTGC 
     1561 CCGTGCGCAG CCTGCTGGCC ATAGGCTTTG GGCTCGTGGT GGCTGCGTCG CACTTGCTGG 
     1621 TCACAGCCAC CTTGGTCCCC GCCAAGCGCC CACGTCTCTG GAGGACGCTC GGTGCCAATG 
     1681 CCTTGCTCTT CGTCGGTGTG AACATGTATG GGGTCTTTGT GCGGATTCTG ACTGAGCGTT 
     1741 CACAGAGGAA GGCGTTCCTG CAGGCCCGGA GCTGCATTGA GGACCGACTG AGGCTGGAGG 
     1801 ATGAGAACGA GAAGCAGGAG CGGCTCCTCA TGAGCCTCCT GCCCCGGAAC GTTGCCATGG 
     1861 AGATGAAGGA GGACTTCCTG AAGCCCCCTG AGAGGATTTT CCACAAGATT TACATCCAGA 
     1921 GGCACGACAA TGTGAGCATC CTGTTTGCTG ACATCGTGGG TTTCACGGGC TTGGCATCCC 
     1981 AGTGCACAGC CCAGGAGCTG GTGAAACTCC TCAATGAGCT CTTCGGCAAG TTCGATGAAT 
     2041 TAGCCACGGA GAACCACTGT CGCCGCATCA AGATTCTCGG GGACTGCTAC TACTGCGTGT 
     2101 CGGGCCTCAC CCAGCCCAAG ACTGACCATG CCCACTGCTG TGTGGAGATG GGACTCGACA 
     2161 TGATTGATAC CATCACATCT GTGGCTGAAG CCACCGAGGT GGATCTGAAC ATGCGTGTGG 
     2221 GTCTGCACAC GGGCAGGGTC CTCTGTGGTG TCCTGGGCTT GCGCAAGTGG CAGTACGACG 
     2281 TGTGGTCCAA TGATGTGACC TTGGCCAATG TCATGGAAGC CGCTGGCCTG CCAGGGAAGG 
     2341 TTCATATCAC AAAGACGACC CTAGCGTGCT TGAATGGGGA CTACGAGGTA GAACCGGGTT 
     2401 ACGGACATGA GAGGAACAGT TTCTTGAAAA CTCATAACAT CGAAACCTTT TTTATTGTGC 
     2461 CATCCCATCG CCGAAAGATA TTTCCAGGCC TGATTCTCTC AGATATAAAA CCGGCCAAAA 
     2521 GGATGAAGTT CAAGACTGTC TGCTACCTGC TGGTGCAGCT CATGCACTGC CGGAAAATGT 
     2581 TCAAGGCCGA GATCCCCTTC TCCAATGTCA TGACCTGCGA GGACGATGAC AAGCGGAGGG 
     2641 CATTAAGAAC AGCCTCGGAA AAACTCAGAA ACCGCTCATC TTTTTCTACC AACGTTGTCT 
     2701 ACACCACCCC GGGCACTCGC GTCAACAGGT ACATCAGCCG CCTCTTAGAA GCCCGCCAGA 
     2761 CAGAGCTGGA GATGGCAGAC CTGAACTTCT TTACCCTGAA GTACAAACAT GTCGAACGGG 
     2821 AGCAAAAGTA CCACCAGCTT CAGGACGAGT ATTTCACCAG CGCCGTTGTC CTCACCCTCA 
     2881 TCCTGGCTGC CTTATTTGGC CTTGTCTACC TTCTAATATT CCCACAGAGT GTGGTCGTCC 
     2941 TGCTCCTGCT AGTATTCTGC ATCTGCTTCC TGGTGGCCTG TGTCCTGTAC CTGCACATCA 
     3001 CCCGGGTCCA GTGTTTTCCA GGGTGCCTGA CGATTCAGAT TCGCACTGTC CTGTGTATTT 
     3061 TCATAGTGGT CTTAATCTAC TCAGTAGCCC AAGGTTGTGT GGTGGGCTGC CTGCCTTGGG 
     3121 CCTGGAGCTC CAAGCCCAAC AGTTCCCTGG TGGTCCTTTC GTCTGGGGGC CAGCGCACAG 
     3181 CCCTGCCCAC CCTGCCCTGC GAGTCTACAC ACCATGCCCT GCTCTGCTGC CTGGTGGGCA 
     3241 CCCTCCCGCT AGCCATATTT TTCCGGGTGT CCTCCTTGCC AAAAATGATC CTGCTCTCCG 
     3301 GGCTCACCAC GTCCTACATC CTCGTTCTGG AGCTCAGCGG ATACACCAGG ACTGGGGGTG 
     3361 GTGCCGTCTC CGGGCGCAGC TACGAGCCGA TTGTGGCCAT CCTGCTCTTC TCCTGTGCGC 
     3421 TGGCCCTGCA TGCCAGGCAG GTGGACATCA GGCTGAGGCT GGACTACCTC TGGGCCGCAC 
     3481 AGGCAGAGGA GGAGCGAGAG GACATGGAGA AGGTGAAGCT GGACAACAGG CGCATCCTCT 
     3541 TCAACCTCCT GCCGGCCCAC GTCGCCCAGC ACTTCCTCAT GTCCAACCCT CGGAACATGG 
     3601 ACCTCTACTA CCAGTCCTAC TCCCAGGTGG GCGTCATGTT TGCCTCCATC CCCAACTTCA 
     3661 ATGACTTCTA CATCGAGCTG GACGGCAACA ACATGGGGGT GGAGTGTCTG CGGCTTCTCA 
     3721 ACGAGATCAT CGCCGACTTT GACGAGCTCA TGGAAAAAGA CTTTTACAAG GACATAGAGA 
     3781 AGATCAAGAC CATCGGGAGC ACCTACATGG CCGCTGTGGG GCTAGCGCCC ACCTCGGGGA 
     3841 CCAAGGCTAA GAAGTCCATC TCCTCCCACC TGAGCACGCT GGCGGACTTT GCCATTGAGA 
     3901 TGTTTGACGT TCTGGATGAA ATCAACTACC AGTCTTACAA CGACTTTGTC CTCCGAGTTG 
     3961 GCATCAATGT TGGCCCTGTG GTGGCTGGAG TGATTGGCGC TCGCAGGCCC CAGTACGACA 
     4021 TCTGGGGAAA CACAGTCAAC GTGGCCAGTC GGATGGATAG CACAGGGGTC CAGGGCAGAA 
     4081 TCCAGGTGAC TGAGGAAGTC CACCGGCTGC TGAGAAGGTG CCCCTACCAC TTTGTGTGCC 
     4141 GAGGCAAAGT CAGTGTCAAG GGCAAAGGCG AGATGTTGAC ATACTTTCTA GAAGGCAGGA 
     4201 CTGATGGAAA CGGCTCCCAA ATCAGGTCCC TGGGCTTGGA TCGGAAAATG TGTCCATTTG 
     4261 GGAGAGCTGG CCTTCAGGGC AGACGTCCCC CCGTGTGCCC CATGCCTGGC GTCTCAGTCA 
     4321 GGGCTGGGCT CCCTCCACAC TCCCCAGGCC AGTACCTGCC CTCTGCAGCA GCTGGGAAGG 
     4381 AGGCTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4441 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4501 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4561 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4621 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4681 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4741 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4801 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4861 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4921 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4981 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5041 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5101 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5161 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5221 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5281 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5341 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5401 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5461 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5521 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5581 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5641 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5701 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5761 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5821 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5881 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5941 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6001 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6061 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6121 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6181 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6241 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6301 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6361 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6421 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6481 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6541 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6601 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6661 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6721 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6781 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6841 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6901 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6961 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7021 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7081 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7141 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7201 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7261 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7321 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7381 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7441 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7501 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7561 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7621 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7681 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7741 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7801 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7861 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7921 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7981 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8041 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8101 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8161 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8221 AAATTTAACG CGAATTTT
//