LOCUS       RC222621L1                9063 bp    DNA     circular  SYN 
DEFINITION  CLCN2 (Myc-DDK-tagged)-Human chloride channel 2 (CLCN2), transcript variant 1
ACCESSION   NM_004366
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7354..7534 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8262..8921 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7693..8261 
                     /label="ColE1"  
     misc_feature    6229..6249 
                     /label="LR50 primer"  
     misc_feature    6026..6097 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6238..6829 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6008..6014 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6008 
                     /label="CLCN2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCGGCCGCG GCGGCGGAGG AAGGGATGGA 
     3361 GCCACGGGCG CTGCAGTACG AGCAGACCCT GATGTATGGC CGGTACACTC AGGACCTTGG 
     3421 GGCCTTTGCC AAAGAGGAAG CTGCTCGGAT TCGCCTGGGA GGGCCTGAAC CCTGGAAAGG 
     3481 TCCCCCTTCC TCTCGGGCTG CCCCAGAGCT CTTGGAATAT GGACGGAGCC GTTGCGCCCG 
     3541 ATGCCGCGTC TGTTCTGTCC GCTGCCACAA GTTCCTAGTA TCCAGGGTTG GTGAAGATTG 
     3601 GATCTTCCTG GTCCTGCTGG GGCTTCTCAT GGCATTGGTC AGCTGGGTCA TAGACTATGC 
     3661 CATTGCTGCC TGTCTGCAAG CCCAGCAGTG GATGTCCCGG GGCTTGAACA CCAGCATCTT 
     3721 GCTCCAGTAC CTGGCCTGGG TCACCTACCC TGTTGTCCTC ATCACTTTCT CAGCCGGATT 
     3781 CACACAGATC CTGGCCCCTC AGGCTGTCGG CTCTGGCATC CCTGAGATGA AGACCATCTT 
     3841 GCGGGGAGTG GTGCTGAAAG AATACCTCAC ACTCAAGACC TTTATAGCTA AGGTCATTGG 
     3901 GCTGACCTGC GCCCTAGGCA GCGGGATGCC GCTTGGCAAA GAGGGCCCTT TTGTGCATAT 
     3961 CGCAAGCATG TGTGCTGCCC TTCTCAGCAA GTTCCTCTCC CTCTTTGGGG GTATCTATGA 
     4021 GAATGAATCC CGGAACACAG AGATGCTGGC TGCCGCCTGT GCCGTGGGGG TGGGCTGCTG 
     4081 CTTCGCGGCA CCTATTGGAG GCGTCCTCTT CAGCATCGAG GTCACCTCCA CCTTCTTTGC 
     4141 AGTGCGGAAC TACTGGCGGG GCTTCTTCGC TGCCACCTTC AGTGCCTTCA TCTTCCGGGT 
     4201 CTTGGCAGTC TGGAACCGGG ATGAAGAGAC TATTACAGCC CTCTTCAAAA CCCGATTCCG 
     4261 GCTCGACTTC CCCTTTGACC TGCAGGAGCT GCCAGCCTTT GCTGTCATTG GTATTGCTAG 
     4321 TGGCTTCGGT GGAGCCCTCT TTGTCTACCT GAACCGGAAG ATTGTCCAGG TGATGCGGAA 
     4381 GCAGAAAACC ATCAATCGCT TCCTCATGAG GAAACGCCTG CTCTTCCCGG CTCTGGTGAC 
     4441 CCTGCTCATC TCCACGCTGA CCTTCCCCCC TGGCTTTGGA CAGTTCATGG CTGGACAGCT 
     4501 CTCACAGAAA GAGACGCTGG TCACCCTGTT TGACAATCGG ACGTGGGTCC GCCAGGGCCT 
     4561 GGTGGAGGAG CTAGAACCAC CCAGCACCTC ACAGGCCTGG AACCCACCAC GTGCCAACGT 
     4621 CTTCCTCACC CTGGTCATCT TCATTCTCAT GAAGTTCTGG ATGTCTGCAC TGGCCACCAC 
     4681 CATCCCAGTT CCCTGTGGGG CCTTCATGCC TGTCTTTGTC ATTGGAGCAG CATTTGGGCG 
     4741 TCTGGTGGGT GAAAGCATGG CTGCCTGGTT CCCAGATGGA ATTCATACGG ACAGCAGCAC 
     4801 CTACCGGATT GTGCCTGGGG GCTACGCTGT GGTCGCTGCG CTGGCAGGAG CGGTGACACA 
     4861 CACAGTGTCC ACGGCTGTGA TCGTGTTCGA GCTCACAGGC CAGATTGCCC ACATCCTGCC 
     4921 TGTCATGATC GCCGTCATCC TGGCCAACGC TGTCGCCCAG AGTCTGCAGC CCTCCCTCTA 
     4981 TGACAGCATC ATCCGAATCA AGAAACTGCC CTACCTGCCT GAGCTCGGCT GGGGCCGCCA 
     5041 CCAGCAGTAC CGGGTGCGTG TGGAGGACAT CATGGTGCGG GATGTTCCCC ATGTGGCCCT 
     5101 CAGCTGCACC TTCCGGGACC TGCGTTTGGC ACTGCACAGG ACCAAGGGCC GAATGCTGGC 
     5161 CCTAGTGGAG TCCCCTGAGT CCATGATTCT GCTGGGCTCC ATCGAGCGTT CACAGGTGGT 
     5221 GGCATTGTTG GGGGCCCAGC TGAGCCCAGC CCGCCGGCGG CAGCACATGC AGGAGCGCAG 
     5281 AGCCACCCAG ACCTCTCCAC TATCTGATCA GGAGGGTCCC CCTAGCCCTG AGGCTTCTGT 
     5341 CTGCTTCCAG GTGAACACAG AAGACTCAGC CTTCCCAGCA GCCCGGGGGG AGACCCACAA 
     5401 GCCCCTAAAG CCTGCACTCA AGAGGGGGCC CAGTGTCACC AGGAACCTCG GAGAGAGTCC 
     5461 CACAGGGAGC GCAGAGTCGG CAGGCATCGC CCTCCGGAGC CTCTTCTGTG GCAGTCCACC 
     5521 CCCTGAGGCT GCTTCGGAGA AGTTGGAATC CTGTGAGAAG CGCAAGCTGA AGCGTGTCCG 
     5581 AATCTCCCTG GCAAGTGACG CGGACCTGGA AGGCGAGATG AGCCCTGAAG AGATTCTGGA 
     5641 GTGGGAGGAG CAGCAACTAG ATGAACCTGT CAACTTCAGT GACTGCAAAA TTGATCCTGC 
     5701 TCCCTTCCAG CTGGTGGAGC GGACCTCTTT GCACAAGACT CACACTATCT TCTCACTGCT 
     5761 GGGAGTGGAC CATGCTTATG TCACCAGTAT TGGCAGACTC ATTGGAATCG TTACTCTAAA 
     5821 GGAGCTCCGG AAGGCCATCG AGGGCTCTGT CACAGCACAG GGTGTGAAAG TCCGGCCGCC 
     5881 CCTCGCCAGC TTCCGAGACA GTGCCACCAG CAGCAGTGAC ACGGAGACCA CTGAGGTGCA 
     5941 TGCACTCTGG GGGCCCCACT CCCGTCATGG CCTCCCCCGG GAGGGCAGCC CTTCCGACAG 
     6001 CGACGACAAA TGCCAAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA 
     6061 TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG 
     6121 CCGCGGTCTG TACAAGTAGG ATTCGTCGAG GGACCTAATA ACTTCGTATA GCATACATTA 
     6181 TACGAAGTTA TACATGTTTA AGGGTTCCGG TTCCACTAGG TACAATTCGA TATCAAGCTT 
     6241 ATCGATAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT 
     6301 GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT 
     6361 TCCCGTATGG CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG 
     6421 GAGTTGTGGC CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC 
     6481 CCCACTGGTT GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC 
     6541 CTCCCTATTG CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT 
     6601 CGGCTGTTGG GCACTGACAA TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG 
     6661 CTGCTCGCCT GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG 
     6721 GCCCTCAATC CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG 
     6781 CGTCTTCGCC TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCATCGA 
     6841 TACCGTCGAC CTCGATCGAG ACCTAGAAAA ACATGGAGCA ATCACAAGTA GCAATACAGC 
     6901 AGCTACCAAT GCTGATTGTG CCTGGCTAGA AGCACAAGAG GAGGAGGAGG TGGGTTTTCC 
     6961 AGTCACACCT CAGGTACCTT TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA 
     7021 CTTTTTAAAA GAAAAGGGGG GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATAT 
     7081 CCTTGATCTG TGGATCTACC ACACACAAGG CTACTTCCCT GATTGGCAGA ACTACACACC 
     7141 AGGGCCAGGG ATCAGATATC CACTGACCTT TGGATGGTGC TACAAGCTAG TACCAGTTGA 
     7201 GCAAGAGAAG GTAGAAGAAG CCAATGAAGG AGAGAACACC CGCTTGTTAC ACCCTGTGAG 
     7261 CCTGCATGGG ATGGATGACC CGGAGAGAGA AGTATTAGAG TGGAGGTTTG ACAGCCGCCT 
     7321 AGCATTTCAT CACATGGCCC GAGAGCTGCA TCCGGACTGT ACTGGGTCTC TCTGGTTAGA 
     7381 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 
     7441 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 
     7501 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGCATGT GAGCAAAAGG 
     7561 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     7621 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     7681 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     7741 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     7801 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     7861 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     7921 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     7981 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     8041 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     8101 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     8161 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     8221 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA TTACGCCCCG 
     8281 CCCTGCCACT CATCGCAGTA CTGTTGTAAT TCATTAAGCA TTCTGCCGAC ATGGAAGCCA 
     8341 TCACAAACGG CATGATGAAC CTGAATCGCC AGCGGCATCA GCACCTTGTC GCCTTGCGTA 
     8401 TAATATTTGC CCATGGTGAA AACGGGGGCG AAGAAGTTGT CCATATTGGC CACGTTTAAA 
     8461 TCAAAACTGG TGAAACTCAC CCAGGGATTG GCTGAGACGA AAAACATATT CTCAATAAAC 
     8521 CCTTTAGGGA AATAGGCCAG GTTTTCACCG TAACACGCCA CATCTTGCGA ATATATGTGT 
     8581 AGAAACTGCC GGAAATCGTC GTGGTATTCA CTCCAGAGCG ATGAAAACGT TTCAGTTTGC 
     8641 TCATGGAAAA CGGTGTAACA AGGGTGAACA CTATCCCATA TCACCAGCTC ACCGTCTTTC 
     8701 ATTGCCATAC GGAACTCCGG ATGAGCATTC ATCAGGCGGG CAAGAATGTG AATAAAGGCC 
     8761 GGATAAAACT TGTGCTTATT TTTCTTTACG GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA 
     8821 ACGGTCTGGT TATAGGTACA TTGAGCAACT GACTGAAATG CCTCAAAATG TTCTTTACGA 
     8881 TGCCATTGGG ATATATCAAC GGTGGTATAT CCAGTGATTT TTTTCTCCAT ACTCTTCCTT 
     8941 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     9001 TGTATTTAGA AAAATAAACA AATAGGGGTC CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     9061 GAC
//