LOCUS       RC222621                7569 bp    DNA     circular  SYN 
DEFINITION  CLCN2 (Myc-DDK-tagged)-Human chloride channel 2 (CLCN2), transcript variant 1
ACCESSION   NM_004366
VERSION     NM_004366.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3726..3817 
                     /label="Myc-DDK"  
     misc_feature    3847..4432 
                     /label="PolyA signal"  
     misc_feature    3904..3923 
                     /label="Restriction Enzyme"  
     misc_feature    4708..5380 
                     /label="ColE1"  
     misc_feature    5810..6604 
                     /label="Kan/Neo"  
     misc_feature    6639..6941 
                     /label="SV40 ori"  
     misc_feature    7027..7504 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3708..3714 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3708 
                     /label="CLCN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCGCG GCGGCGGAGG AAGGGATGGA GCCACGGGCG CTGCAGTACG 
     1081 AGCAGACCCT GATGTATGGC CGGTACACTC AGGACCTTGG GGCCTTTGCC AAAGAGGAAG 
     1141 CTGCTCGGAT TCGCCTGGGA GGGCCTGAAC CCTGGAAAGG TCCCCCTTCC TCTCGGGCTG 
     1201 CCCCAGAGCT CTTGGAATAT GGACGGAGCC GTTGCGCCCG ATGCCGCGTC TGTTCTGTCC 
     1261 GCTGCCACAA GTTCCTAGTA TCCAGGGTTG GTGAAGATTG GATCTTCCTG GTCCTGCTGG 
     1321 GGCTTCTCAT GGCATTGGTC AGCTGGGTCA TAGACTATGC CATTGCTGCC TGTCTGCAAG 
     1381 CCCAGCAGTG GATGTCCCGG GGCTTGAACA CCAGCATCTT GCTCCAGTAC CTGGCCTGGG 
     1441 TCACCTACCC TGTTGTCCTC ATCACTTTCT CAGCCGGATT CACACAGATC CTGGCCCCTC 
     1501 AGGCTGTCGG CTCTGGCATC CCTGAGATGA AGACCATCTT GCGGGGAGTG GTGCTGAAAG 
     1561 AATACCTCAC ACTCAAGACC TTTATAGCTA AGGTCATTGG GCTGACCTGC GCCCTAGGCA 
     1621 GCGGGATGCC GCTTGGCAAA GAGGGCCCTT TTGTGCATAT CGCAAGCATG TGTGCTGCCC 
     1681 TTCTCAGCAA GTTCCTCTCC CTCTTTGGGG GTATCTATGA GAATGAATCC CGGAACACAG 
     1741 AGATGCTGGC TGCCGCCTGT GCCGTGGGGG TGGGCTGCTG CTTCGCGGCA CCTATTGGAG 
     1801 GCGTCCTCTT CAGCATCGAG GTCACCTCCA CCTTCTTTGC AGTGCGGAAC TACTGGCGGG 
     1861 GCTTCTTCGC TGCCACCTTC AGTGCCTTCA TCTTCCGGGT CTTGGCAGTC TGGAACCGGG 
     1921 ATGAAGAGAC TATTACAGCC CTCTTCAAAA CCCGATTCCG GCTCGACTTC CCCTTTGACC 
     1981 TGCAGGAGCT GCCAGCCTTT GCTGTCATTG GTATTGCTAG TGGCTTCGGT GGAGCCCTCT 
     2041 TTGTCTACCT GAACCGGAAG ATTGTCCAGG TGATGCGGAA GCAGAAAACC ATCAATCGCT 
     2101 TCCTCATGAG GAAACGCCTG CTCTTCCCGG CTCTGGTGAC CCTGCTCATC TCCACGCTGA 
     2161 CCTTCCCCCC TGGCTTTGGA CAGTTCATGG CTGGACAGCT CTCACAGAAA GAGACGCTGG 
     2221 TCACCCTGTT TGACAATCGG ACGTGGGTCC GCCAGGGCCT GGTGGAGGAG CTAGAACCAC 
     2281 CCAGCACCTC ACAGGCCTGG AACCCACCAC GTGCCAACGT CTTCCTCACC CTGGTCATCT 
     2341 TCATTCTCAT GAAGTTCTGG ATGTCTGCAC TGGCCACCAC CATCCCAGTT CCCTGTGGGG 
     2401 CCTTCATGCC TGTCTTTGTC ATTGGAGCAG CATTTGGGCG TCTGGTGGGT GAAAGCATGG 
     2461 CTGCCTGGTT CCCAGATGGA ATTCATACGG ACAGCAGCAC CTACCGGATT GTGCCTGGGG 
     2521 GCTACGCTGT GGTCGCTGCG CTGGCAGGAG CGGTGACACA CACAGTGTCC ACGGCTGTGA 
     2581 TCGTGTTCGA GCTCACAGGC CAGATTGCCC ACATCCTGCC TGTCATGATC GCCGTCATCC 
     2641 TGGCCAACGC TGTCGCCCAG AGTCTGCAGC CCTCCCTCTA TGACAGCATC ATCCGAATCA 
     2701 AGAAACTGCC CTACCTGCCT GAGCTCGGCT GGGGCCGCCA CCAGCAGTAC CGGGTGCGTG 
     2761 TGGAGGACAT CATGGTGCGG GATGTTCCCC ATGTGGCCCT CAGCTGCACC TTCCGGGACC 
     2821 TGCGTTTGGC ACTGCACAGG ACCAAGGGCC GAATGCTGGC CCTAGTGGAG TCCCCTGAGT 
     2881 CCATGATTCT GCTGGGCTCC ATCGAGCGTT CACAGGTGGT GGCATTGTTG GGGGCCCAGC 
     2941 TGAGCCCAGC CCGCCGGCGG CAGCACATGC AGGAGCGCAG AGCCACCCAG ACCTCTCCAC 
     3001 TATCTGATCA GGAGGGTCCC CCTAGCCCTG AGGCTTCTGT CTGCTTCCAG GTGAACACAG 
     3061 AAGACTCAGC CTTCCCAGCA GCCCGGGGGG AGACCCACAA GCCCCTAAAG CCTGCACTCA 
     3121 AGAGGGGGCC CAGTGTCACC AGGAACCTCG GAGAGAGTCC CACAGGGAGC GCAGAGTCGG 
     3181 CAGGCATCGC CCTCCGGAGC CTCTTCTGTG GCAGTCCACC CCCTGAGGCT GCTTCGGAGA 
     3241 AGTTGGAATC CTGTGAGAAG CGCAAGCTGA AGCGTGTCCG AATCTCCCTG GCAAGTGACG 
     3301 CGGACCTGGA AGGCGAGATG AGCCCTGAAG AGATTCTGGA GTGGGAGGAG CAGCAACTAG 
     3361 ATGAACCTGT CAACTTCAGT GACTGCAAAA TTGATCCTGC TCCCTTCCAG CTGGTGGAGC 
     3421 GGACCTCTTT GCACAAGACT CACACTATCT TCTCACTGCT GGGAGTGGAC CATGCTTATG 
     3481 TCACCAGTAT TGGCAGACTC ATTGGAATCG TTACTCTAAA GGAGCTCCGG AAGGCCATCG 
     3541 AGGGCTCTGT CACAGCACAG GGTGTGAAAG TCCGGCCGCC CCTCGCCAGC TTCCGAGACA 
     3601 GTGCCACCAG CAGCAGTGAC ACGGAGACCA CTGAGGTGCA TGCACTCTGG GGGCCCCACT 
     3661 CCCGTCATGG CCTCCCCCGG GAGGGCAGCC CTTCCGACAG CGACGACAAA TGCCAAACGC 
     3721 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3781 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3841 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3901 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3961 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4021 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4081 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4141 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4201 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4261 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4321 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4381 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4441 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4501 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4561 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4621 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4681 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4741 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4801 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4861 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4921 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4981 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5041 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5101 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5161 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5221 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5281 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5341 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5401 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5461 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5521 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5581 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5641 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5701 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5761 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5821 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5881 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5941 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6001 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6061 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6121 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6181 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6241 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6301 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6361 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6421 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6481 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6541 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6601 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6661 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6721 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6781 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6841 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6901 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6961 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7021 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7081 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7141 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7201 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7261 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7321 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7381 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7441 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7501 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7561 GCGAATTTT
//