LOCUS       RC222609L1                8844 bp    DNA     circular  SYN 
DEFINITION  USP16 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 16 (USP16), transcript variant 1
ACCESSION   NM_006447
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7135..7315 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8043..8702 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7474..8042 
                     /label="ColE1"  
     misc_feature    6010..6030 
                     /label="LR50 primer"  
     misc_feature    5807..5878 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6019..6610 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5789..5795 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5789 
                     /label="USP16"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGAAAGAAA CGGACAAAGG GAAAAACTGT 
     3361 TCCAATCGAT GATTCCTCTG AAACTTTAGA ACCTGTGTGC AGACACATTA GAAAAGGATT 
     3421 GGAACAAGGT AATTTGAAAA AGGCTTTAGT GAATGTGGAA TGGAATATCT GCCAAGACTG 
     3481 TAAGACTGAC AATAAAGTGA AAGATAAAGC TGAAGAAGAA ACAGAAGAAA AGCCTTCAGT 
     3541 TTGGCTGTGT CTTAAATGTG GCCATCAGGG CTGTGGCAGA AATTCTCAGG AGCAGCATGC 
     3601 CTTGAAGCAC TATCTGACGC CAAGATCTGA ACCTCACTGT CTGGTTCTTA GTTTGGACAA 
     3661 CTGGAGTGTA TGGTGTTACG TATGTGATAA TGAGGTCCAG TATTGTAGTT CAAACCAGTT 
     3721 GGGTCAAGTG GTTGATTATG TCAGAAAACA AGCCAGCATT ACAACTCCAA AGCCAGCAGA 
     3781 GAAAGATAAT GGAAATATTG AACTTGAAAA TAAAAAATTA GAAAAAGAGA GTAAGAATGA 
     3841 ACAAGAGAGA GAAAAGAAGG AAAACATGGC TAAAGAGAAT CCTCCCATGA ATTCTCCTTG 
     3901 CCAAATAACC GTGAAAGGAC TCAGTAATTT GGGAAACACA TGTTTCTTCA ATGCAGTTAT 
     3961 GCAGAACTTG TCACAAACAC CAGTGCTTAG AGAACTACTA AAAGAAGTGA AAATGTCTGG 
     4021 AACAATTGTA AAAATTGAAC CACCTGATTT GGCATTAACA GAACCATTAG AAATAAACCT 
     4081 TGAGCCTCCA GGCCCTCTTA CTTTAGCCAT GAGCCAGTTT CTTAATGAGA TGCAAGAGAC 
     4141 CAAAAAGGGG GTTGTGACAC CGAAAGAACT CTTTTCTCAG GTCTGTAAAA AAGCAGTGCG 
     4201 GTTTAAAGGC TATCAGCAGC AAGACAGCCA GGAGCTGCTT CGCTACTTAT TGGATGGGAT 
     4261 GAGAGCAGAA GAACACCAAA GAGTGAGTAA AGGAATACTT AAAGCATTTG GTAATTCTAC 
     4321 TGAAAAGTTG GATGAAGAAC TAAAAAATAA AGTTAAAGAT TATGAGAAGA AAAAATCAAT 
     4381 GCCAAGTTTT GTTGACCGCA TCTTTGGTGG TGAACTAACT AGTATGATCA TGTGTGATCA 
     4441 ATGCAGAACT GTCTCCTTGG TTCATGAATC TTTCCTTGAT TTGTCCCTCC CAGTTTTAGA 
     4501 TGATCAGAGT GGTAAGAAAA GTGTAAATGA TAAAAATCTG AAAAAGACAG TGGAGGATGA 
     4561 AGATCAAGAT AGTGAGGAAG AAAAAGATAA CGACAGTTAC ATAAAAGAGA GAAGTGATAT 
     4621 TCCTTCTGGA ACAAGTAAGC ACTTACAGAA AAAAGCAAAG AAACAAGCCA AAAAGCAAGC 
     4681 CAAGAACCAA CGAAGACAAC AAAAAATTCA AGGAAAAGTT CTTCATTTAA ATGATATTTG 
     4741 TACTATTGAC CATCCTGAAG ACAGTGAATA TGAAGCTGAA ATGTCACTTC AAGGAGAAGT 
     4801 AAATATTAAA TCCAACCATA TTTCACAAGA GGGTGTTATG CATAAAGAAT ATTGTGTCAA 
     4861 CCAGAAAGAT TTGAATGGCC AAGCAAAAAT GATCGAAAGT GTAACTGACA ATCAAAAATC 
     4921 CACAGAGGAA GTAGATATGA AAAATATCAA CATGGATAAT GATCTGGAGG TTTTAACATC 
     4981 TTCTCCCACT AGGAATTTAA ATGGTGCCTA CCTAACGGAA GGGAGCAATG GAGAAGTGGA 
     5041 CATTTCCAAT GGTTTCAAAA ACCTAAATTT GAATGCTGCT CTTCATCCTG ATGAAATAAA 
     5101 TATAGAGATT CTGAATGATA GTCATACTCC TGGAACAAAG GTGTATGAGG TTGTAAATGA 
     5161 AGATCCAGAA ACTGCTTTCT GTACTCTTGC AAACAGGGAA GTTTTCAATA CTGATGAGTG 
     5221 TTCAATCCAA CATTGTTTAT ATCAGTTCAC CCGTAATGAG AAACTTCGAG ATGCGAATAA 
     5281 ACTGCTTTGT GAAGTATGCA CACGGAGACA GTGTAATGGA CCAAAGGCAA ATATAAAAGG 
     5341 TGAAAGGAAG CATGTTTACA CCAATGCCAA AAAGCAGATG CTAATTTCTC TTGCTCCTCC 
     5401 TGTTCTTACT CTTCATTTAA AGAGATTTCA GCAGGCTGGT TTTAACCTAC GCAAAGTTAA 
     5461 CAAACACATA AAGTTTCCGG AAATCTTAGA TTTGGCTCCT TTTTGCACCC TTAAATGTAA 
     5521 GAATGTTGCA GAAGAAAATA CAAGGGTACT CTATTCCTTA TATGGAGTTG TTGAACACAG 
     5581 TGGTACTATG AGGTCGGGGC ATTACACTGC CTATGCCAAG GCAAGAACCG CAAATAGTCA 
     5641 TCTCTCTAAT CTTGTTCTTC ACGGTGATAT TCCACAAGAT TTTGAAATGG AATCAAAAGG 
     5701 GCAGTGGTTT CACATCAGCG ACACACATGT GCAAGCTGTG CCTACAACTA AAGTACTAAA 
     5761 CTCACAAGCG TACCTCCTAT TTTATGAGAG AATACTGACG CGTACGCGGC CGCTCGAGCA 
     5821 GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA 
     5881 CGATAAGGTT TAAACGGCCG GCCGCGGTCT GTACAAGTAG GATTCGTCGA GGGACCTAAT 
     5941 AACTTCGTAT AGCATACATT ATACGAAGTT ATACATGTTT AAGGGTTCCG GTTCCACTAG 
     6001 GTACAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 
     6061 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 
     6121 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 
     6181 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 
     6241 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 
     6301 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 
     6361 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 
     6421 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG 
     6481 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 
     6541 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 
     6601 TGGGCCGCCT CCCCGCATCG ATACCGTCGA CCTCGATCGA GACCTAGAAA AACATGGAGC 
     6661 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA 
     6721 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA 
     6781 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 
     6841 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC 
     6901 TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG 
     6961 CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC 
     7021 CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA 
     7081 GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG 
     7141 TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA 
     7201 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT 
     7261 GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC 
     7321 TAGCAGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     7381 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     7441 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     7501 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     7561 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     7621 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     7681 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     7741 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     7801 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     7861 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     7921 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     7981 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     8041 TTTGGTCATG ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC 
     8101 ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC 
     8161 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG 
     8221 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG 
     8281 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC 
     8341 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC 
     8401 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT 
     8461 ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG 
     8521 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA 
     8581 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT 
     8641 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT 
     8701 TTTTTCTCCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8761 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT CCCGCGCACA 
     8821 TTTCCCCGAA AAGTGCCACC TGAC
//