LOCUS       RC222601L1                9372 bp    DNA     circular  SYN 
DEFINITION  ANO2 (Myc-DDK-tagged)-Human anoctamin 2 (ANO2)
ACCESSION   NM_020373
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7663..7843 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8571..9230 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    8002..8570 
                     /label="ColE1"  
     misc_feature    6538..6558 
                     /label="LR50 primer"  
     misc_feature    6335..6406 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6547..7138 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6317..6323 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6317 
                     /label="ANO2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCTGAAGAT ATACCCCTGC TCCCTGGCTC 
     3361 CCCACGCCGG CTGAGCCCTC AGGCAGGGTC CAGAGGGGGC CAGGGCCCCA AACATGGACA 
     3421 GCAGTGTCTC AAGATGCCAG GTCCCCGGGC CCCAGGTCTG CAGGGCGGTT CCAACAGAGA 
     3481 TCCTGGCCAG CCCTGCGGTG GAGAGAGCAC CCGCAGCAGC TCTGTCATCA ACAACTATCT 
     3541 GGATGCCAAT GAGCCTGTGT CCTTGGAGGC CCGTCTTAGC CGCATGCACT TCCATGACAG 
     3601 TCAGAGGAAG GTCGACTATG TACTTGCCTA CCACTACCGG AAACGCGGGG TGCACCTGGC 
     3661 CCAAGGCTTC CCTGGCCACT CGCTGGCTAT CGTCTCCAAT GGGGAGACAG GCAAGGAGCC 
     3721 TCATGCTGGG GGCCCAGGTG ACATTGAGCT GGGACCGCTC GATGCCCTGG AGGAGGAGAG 
     3781 GAAGGAGCAG CGGGAGGAAT TTGAGCACAA TCTGATGGAG GCTGGACTGG AGCTTGAGAA 
     3841 GGACTTGGAG AATAAAAGCC AGGGATCCAT CTTTGTCCGG ATACACGCCC CGTGGCAGGT 
     3901 GCTGGCCAGA GAGGCAGAAT TCTTGAAGAT CAAAGTTCCT ACCAAGAAAG AGATGTACGA 
     3961 GATCAAAGCA GGAGGCAGCA TTGCAAAGAA GTTCAGCGCG GCTCTGCAGA AGCTGAGCTC 
     4021 GCACCTGCAG CCCCGAGTTC CAGAACACAG CAACAACAAG ATGAAAAACC TCTCCTACCC 
     4081 ATTCTCCAGG GAGAAGATGT ACCTGTACAA CATCCAGGAA AAGGACACCT TCTTTGATAA 
     4141 TGCCACCCGT AGCCGCATTG TGCACGAGAT CCTGAAGCGC ACAGCCTGCT CCCGAGCCAA 
     4201 CAACACGATG GGTATTAACT CTCTGATCGC AAACAATATC TATGAGGCTG CCTACCCTCT 
     4261 TCATGACGGT GAATACGATA GTCCAGAGGA CGATATGAAT GACAGGAAGC TGCTATATCA 
     4321 AGAATGGGCG CGCTATGGAG TGTTCTATAA GTTCCAACCT ATTGACCTCA TCAGAAAGTA 
     4381 TTTTGGAGAA AAAATTGGAC TGTATTTTGC CTGGCTGGGA TTATATACAT CATTCCTCAT 
     4441 CCCATCTTCT GTAATTGGAG TGATTGTGTT TCTTTATGGA TGTGCAACAA TTGAAGAAGA 
     4501 TATTCCCAGC AGAGAGATGT GTGACCAGCA GAATGCCTTC ACCATGTGTC CCCTGTGTGA 
     4561 CAAGTCCTGT GATTACTGGA ACCTCAGCTC AGCCTGTGGG ACCGCGCAGG CCAGCCACCT 
     4621 GTTTGACAAC CCTGCCACCG TCTTCTTCTC TATCTTCATG GCTCTGTGGG CTACCATGTT 
     4681 CCTGGAAAAC TGGAAGAGGC TACAGATGCG ACTGGGCTAC TTTTGGGACC TGACTGGCAT 
     4741 AGAAGAGGAA GAAGAACGTG CCCAGGAACA TTCCAGGCCT GAGTATGAAA CCAAAGTTCG 
     4801 AGAGAAAATG CTAAAGGAGA GCAACCAGTC TGCTGTCCAG AAATTGGAAA CAAACACGAC 
     4861 GGAGTGTGGC GATGAGGATG ATGAAGATAA ACTGACCTGG AAGGATCGTT TCCCAGGTTA 
     4921 CCTGATGAAC TTTGCCTCCA TCTTATTCAT GATTGCCCTG ACATTCTCAA TCGTCTTTGG 
     4981 GGTGATAGTG TATCGAATAA CAACTGCAGC CGCTCTGTCT CTCAATAAGG CTACACGCTC 
     5041 CAATGTCCGG GTGACAGTGA CAGCAACAGC AGTCATCATC AACCTCGTGG TCATCCTCAT 
     5101 CCTGGACGAG ATCTACGGCG CTGTGGCCAA GTGGCTCACC AAAATTGAGG TTCCGAAAAC 
     5161 AGAACAGACT TTTGAAGAGC GCCTGATCCT CAAAGCTTTC TTGCTCAAGT TTGTCAATGC 
     5221 CTACTCCCCC ATCTTCTATG TGGCCTTTTT CAAAGGGAGG TTTGTGGGCA GGCCTGGAAG 
     5281 CTACGTCTAT GTATTCGATG GTTACCGCAT GGAAGAGTGT GCTCCAGGGG GCTGTCTCAT 
     5341 GGAGCTCTGC ATTCAGCTCA GCATCATCAT GTTGGGGAAG CAGTTGATCC AGAACAACAT 
     5401 CTTTGAGATT GGAGTCCCGA AGCTAAAGAA ACTATTTCGA AAGCTGAAAG ATGAGACCGA 
     5461 AGCTGGAGAA ACTGACTCTG CCCATTCGAA ACATCCAGAG CAGTGGGACC TAGACTACAG 
     5521 CTTGGAACCA TACACAGGAC TGACTCCGGA GTACATGGAA ATGATCATCC AGTTTGGTTT 
     5581 TGTCACCCTC TTCGTGGCCT CCTTTCCCCT GGCACCTGTG TTTGCCCTCC TCAACAACGT 
     5641 CATTGAAGTG CGGCTCGATG CAAAGAAGTT TGTTACAGAG CTGAGACGGC CGGATGCTGT 
     5701 AAGAACCAAA GATATCGGAA TCTGGTTTGA CATTCTCTCT GGAATTGGCA AGTTCTCTGT 
     5761 TATCAGCAAC GCTTTTGTCA TTGCGATCAC CTCCGACTTT ATCCCCCGCC TGGTGTACCA 
     5821 GTACTCCTAC AGTCACAATG GGACTCTGCA CGGCTTTGTC AACCACACCC TCTCCTTTTT 
     5881 CAACGTCAGC CAGCTGAAGG AGGGGACGCA GCCAGAAAAC TCACAGTTTG ACCAGGAGGT 
     5941 TCAGTTCTGC AGGTTTAAGG ATTACCGAGA GCCGCCATGG GCCCCGAACC CTTATGAGTT 
     6001 TTCGAAACAG TACTGGTTTA TTCTGTCTGC CCGTCTGGCT TTTGTCATAA TCTTCCAGAA 
     6061 CCTCGTGATG TTCCTGAGCG TCCTCGTGGA CTGGATGATT CCAGACATCC CCACGGACAT 
     6121 CAGCGACCAG ATCAAGAAAG AGAAGAGCTT ATTAGTGGAT TTCTTCCTGA AAGAGGAGCA 
     6181 TGAGAAGCTC AAGCTGATGG ATGAGCCGGC TCTGAGGAGC CCAGGAGGTG GGGATCGAAG 
     6241 CAGGAGCCGG GCAGCCAGCT CAGCACCTTC AGGCCAAAGC CAGCTGGGCA GCATGATGTC 
     6301 GTCAGGCTCT CAGCACACCA ATGTGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC 
     6361 AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA 
     6421 AACGGCCGGC CGCGGTCTGT ACAAGTAGGA TTCGTCGAGG GACCTAATAA CTTCGTATAG 
     6481 CATACATTAT ACGAAGTTAT ACATGTTTAA GGGTTCCGGT TCCACTAGGT ACAATTCGAT 
     6541 ATCAAGCTTA TCGATAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT 
     6601 CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT 
     6661 GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT 
     6721 CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT 
     6781 GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC 
     6841 GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG 
     6901 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC 
     6961 TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC 
     7021 GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG 
     7081 CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC 
     7141 CCGCATCGAT ACCGTCGACC TCGATCGAGA CCTAGAAAAA CATGGAGCAA TCACAAGTAG 
     7201 CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA GCACAAGAGG AGGAGGAGGT 
     7261 GGGTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA 
     7321 TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG 
     7381 ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC TACTTCCCTG ATTGGCAGAA 
     7441 CTACACACCA GGGCCAGGGA TCAGATATCC ACTGACCTTT GGATGGTGCT ACAAGCTAGT 
     7501 ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC CAATGAAGGA GAGAACACCC GCTTGTTACA 
     7561 CCCTGTGAGC CTGCATGGGA TGGATGACCC GGAGAGAGAA GTATTAGAGT GGAGGTTTGA 
     7621 CAGCCGCCTA GCATTTCATC ACATGGCCCG AGAGCTGCAT CCGGACTGTA CTGGGTCTCT 
     7681 CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA 
     7741 GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC 
     7801 TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGCATGTG 
     7861 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     7921 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     7981 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     8041 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     8101 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     8161 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     8221 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     8281 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     8341 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     8401 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     8461 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     8521 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAT 
     8581 TACGCCCCGC CCTGCCACTC ATCGCAGTAC TGTTGTAATT CATTAAGCAT TCTGCCGACA 
     8641 TGGAAGCCAT CACAAACGGC ATGATGAACC TGAATCGCCA GCGGCATCAG CACCTTGTCG 
     8701 CCTTGCGTAT AATATTTGCC CATGGTGAAA ACGGGGGCGA AGAAGTTGTC CATATTGGCC 
     8761 ACGTTTAAAT CAAAACTGGT GAAACTCACC CAGGGATTGG CTGAGACGAA AAACATATTC 
     8821 TCAATAAACC CTTTAGGGAA ATAGGCCAGG TTTTCACCGT AACACGCCAC ATCTTGCGAA 
     8881 TATATGTGTA GAAACTGCCG GAAATCGTCG TGGTATTCAC TCCAGAGCGA TGAAAACGTT 
     8941 TCAGTTTGCT CATGGAAAAC GGTGTAACAA GGGTGAACAC TATCCCATAT CACCAGCTCA 
     9001 CCGTCTTTCA TTGCCATACG GAACTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA 
     9061 ATAAAGGCCG GATAAAACTT GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA 
     9121 TCCAGCTGAA CGGTCTGGTT ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT 
     9181 TCTTTACGAT GCCATTGGGA TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATA 
     9241 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     9301 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTCC CGCGCACATT TCCCCGAAAA 
     9361 GTGCCACCTG AC
//