LOCUS       RC222567                9402 bp    DNA     circular  SYN 
DEFINITION  PRDM15 (Myc-DDK-tagged)-Human PR domain containing 15 (PRDM15), transcript variant 1
ACCESSION   NM_022115
VERSION     NM_022115.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5559..5650 
                     /label="Myc-DDK"  
     misc_feature    5680..6265 
                     /label="PolyA signal"  
     misc_feature    5737..5756 
                     /label="Restriction Enzyme"  
     misc_feature    6541..7213 
                     /label="ColE1"  
     misc_feature    7643..8437 
                     /label="Kan/Neo"  
     misc_feature    8472..8774 
                     /label="SV40 ori"  
     misc_feature    8860..9337 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5541..5547 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5541 
                     /label="PRDM15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCCCGCCGC CGCCCCCCGG CTTCCGGTGC TGCGCAGTTT CCGGAGCGGA 
     1081 TCGCAACCCG GAGTCCGGAT CCGATCCCGC TCTGCACATT CCAAAGGCAR CCGCGCGCCG 
     1141 CCCCGGTCCA GCCGCCATGC CGACTGTTCT TTGTTACATT CGCCGGCTGC GGGCACCGTT 
     1201 GGCGATCGGA GTCAAAACCC GGCTGGATTT CCCGGAGCCG CTCCGGGATC GCCCTGCGCG 
     1261 CCGCCCGCCC GCCGGGGTCT TCGCCGCCCC GGCCCGCGGC CCCGCGGCCC CCGCCGCCGG 
     1321 GCGGCGTCGT CGCCGAGGCC CCAGGGGATG TCGTTATCCC CCGCCCTCGG GTACAGCCCA 
     1381 TGCGGGTCGC ACGGGGGGGT CCCTGGACCC CCAACCCCGC GTTTAGAGAA GCTGAGTCCT 
     1441 GGTCCCAGAT TGGGAACCAG AGGGTCAGTG AGCAGCTCTT GGAAACATCT CTAGGGAATG 
     1501 AGGTGTCCGA CACTGAGCCA CTGAGCCCTG CGAGTGCAGG CCTGCGACGC AATCCTGCCC 
     1561 TACCTCCTGG ACCCTTTGCA CAAAACTTTT CCTGGGGGAA CCAGGAAAAT CTGCCCCCAG 
     1621 CCCTGGGGAA GATTGCGAAC GGAGGAGGAA CTGGGGCAGG CAAGGCCGAA TGCGGCTATG 
     1681 AAACTGAGTC ACACTTGCTA GAGCCGCACG AGATACCTTT GAACGTGAAT ACACACAAGT 
     1741 TCAGTGACTG TGAGTTTCCA TATGAGTTTT GCACGGTCTG CTTTTCACCC TTCAAGCTGC 
     1801 TGGGGATGAG CGGGGTGGAG GGCGTGTGGA ATCAGCATTC AAGAAGTGCC AGCATGCACA 
     1861 CTTTCCTAAA CCACTCAGCA ACGGGCATCA GAGAAGCAGG TTGCAGGAAG GACATGCCSG 
     1921 TGTCAGAGAT GGCTGAAGAT GGGAGCGAAG AGATCATGTT CATCTGGTGT GAAGACTGCA 
     1981 GCCAGTACCA CGACTCCGAA TGTCCCGAGC TGGGCCCAGT GGTCATGGTC AAAGACTCCT 
     2041 TTGTGTTAAG CAGGGCAAGG TCTTGGCCTG CCAGCGGACA CGTGCACACC CAGGCGGGGC 
     2101 AGGGGATGCG GGGTTATGAG GACAGGGACA GGGCTGACCC ACAGCAGCTT CCAGAAGCAG 
     2161 TCCCTGCAGG CCTGGTGAGG CGGCTCAGTG GGCAGCAGCT GCCCTGCCGT TCCACCCTCA 
     2221 CCTGGGGGAG GCTGTGCCAC CTGGTGGCCC AGGGCAGGTC ATCCCTTCCT CCCAACTTGG 
     2281 AGATCAGACG ACTGGAAGAT GGAGCCGAGG GGGTGTTCGC CATCACTCAG CTCGTCAAGC 
     2341 GGACACAGTT CGGTCCCTTT GAGTCCAGGA GGGTCGCCAA ATGGGAAAAG GAGTCTGCAT 
     2401 TTCCCCTGAA GGTGTTCCAG AAGGACGGGC ACCCCGTGTG CTTCGACACC TCCAACGAGG 
     2461 ATGACTGCAA CTGGATGATG CTGGTGCGGC CAGCGGCGGA GGCCGAGCAC CAGAACCTGA 
     2521 CGGCCTACCA GCACGGCAGC GACGTGTACT TCACCACCTC CAGAGACATC CCCCCGGGTA 
     2581 CCGAGCTGCG CGTGTGGTAT GCGGCCTTCT ATGCCAAGAA GATGGACAAG CCCATGCTGA 
     2641 AGCAGGCCGG CTCTGGCGTC CACGCTGCAG GCACCCCAGA AAACAGCGCC CCCGTGGAGT 
     2701 CGGAGCCCAG CCAGTGGGCG TGTAAAGTGT GTTCTGCCAC CTTCCTGGAG CTGCAGCTCC 
     2761 TCAATGAACA TCTGTTGGGC CACTTAGAAC AAGCCAAAAG CCTTCCTCCA GGCAGCCAAA 
     2821 GCGAGGCAGC AGCTCCCGAG AAGGAGCAGG ACACACCCCG GGGGGAACCC CCTGCAGTGC 
     2881 CCGAGAGCGA GAATGTTGCC ACCAAAGAAC AGAAGAAAAA GCCTCGAAGG GGGAGAAAAC 
     2941 CCAAAGTGTC CAAAGCTGAG CAGCCTCTAG TCATCGTGGA AGACAAGGAA CCCACAGAGC 
     3001 AAGTGGCAGA GATCATTACC GAGGTCCCTC CGGATGAGCC TGTGAGTGCA ACGCCAGATG 
     3061 AGCGGATCAT GGAGCTGGTT CTGGGGAAGC TGGCCACCAC CACCACTGAC ACCAGCTCGG 
     3121 TTCCAAAGTT CACCCATCAT CAGAATAACA CCATCACGCT CAAGAGGAGC TTAATTCTCT 
     3181 CAAGCAGACA CGGCATCCGG CGCAAGCTCA TCAAACAGCT CGGGGAGCAC AAGCGGGTTT 
     3241 ACCAGTGCAA TATCTGCAGC AAGATCTTCC AGAACAGCAG CAACCTGAGC AGGCACGTGC 
     3301 GCTCGCATGG TGACAAGCTG TTTAAGTGCG AAGAGTGTGC AAAATTGTTC AGCCGCAAAG 
     3361 AGAGCCTAAA GCAGCACGTT TCCTACAAGC ACAGCAGGAA CGAGGTGGAC GGCGAGTACA 
     3421 GGTACCGCTG CGGCACTTGT GAGAAGACCT TCCGCATCGA GAGCGCGCTG GAGTTCCACA 
     3481 ACTGCAGGAC AGATGACAAG ACGTTCCAAT GTGAGATGTG TTTCAGATTC TTCTCCACCA 
     3541 ACAGCAACCT CTCCAAGCAC AAGAAGAAGC ACGGCGACAA GAAGTTTGCC TGTGAGGTCT 
     3601 GCAGCAAGAT GTTCTACCGC AAGGACGTCA TGCTGGACCA CCAGCGCCGG CACCTGGAAG 
     3661 GAGTGCGGCG AGTGAAGCGA GAGGACCTGG AGGCCGGTGG GGAGAACCTG GTCCGTTACA 
     3721 AGAAGGAGCC TTCCGGGTGC CCGGTGTGTG GCAAGGTGTT CTCCTGCCGG AGCAATATGA 
     3781 ACAAGCACCT GCTCACCCAC GGSGACAAGA AGTACACCTG CGAGATCTGC GGGCGCAAGT 
     3841 TCTTCCGCGT GGATGTGCTC AGGGACCACA TCCATGTCCA CTTCAAGGAC ATCGCGTTGA 
     3901 TGGATGACCA CCAGAGGGAA GAGTTTATCG GCAAGATCGG GATCTCCTCG GAAGAAAACG 
     3961 ATGACAATTC TGACGAGAGC GCAGACTCGG AGCCTCACAA GTACAGCTGC AAGCGGTGCC 
     4021 AGCTCACCTT CGGCCGGGGG AAGGAGTACC TGAAGCACAT CATGGAGGTG CACAAGGAGA 
     4081 AGGGCTWTGG CTGCAGCATC TGCAACCGGC GCTTTGCACT GMAGGCCACC TACCACGCCC 
     4141 ACATGGTCAT CCACCGTGAA AACCTGCCGG ACCCCAACGT GCAGAAGTAC ATCCACCCCT 
     4201 GCGAGATCTG CGGGCGGATC TTCAACAGCA TCGGGAACCT GGAGCGCCAC AAGCTCATCC 
     4261 ACACAGGTGT GAAGAGCCAC GCCTGCGAGC AGTGTGGGAA GTCCTTTGCC AGGAAGGACA 
     4321 TGCTGAAGGA GCACATGCGT GTGCACGACA ACGTCCGCGA GTACCTGTGT GCCGAGTGTG 
     4381 GGAAAGGCAT GAAGACCAAG CACGCGCTGC GCCACCACAT GAAGCTGCAC AAGGGCATCA 
     4441 AGGAGTACGA GTGCAAGGAG TGCCACCGCA GGTTCGCGCA GAAGGTCAAC ATGCTCAAGC 
     4501 ACTGCAAGCG GCACACGGGG ATTAAAGATT TCATGTGTGA ATTGTGTGGG AAGACATTCA 
     4561 GCGAGAGGAA CACCATGGAG ACCCACAAGC TCATCCACAC AGTGGGCAAG CAGTGGACGT 
     4621 GCTCCGTGTG CGACAAGAAG TACGTGACCG AGTACATGCT GCAGAAGCAC GTTCAGCTCA 
     4681 CACACGACAA GGTGGAGGCG CAGAGCTGCC AGCTGTGCGG GACCAAGGTG TCCACCAGGG 
     4741 CCTCCATGAG CCGACACATG CGGCGCAAGC ACCCCGAGGT GCTCGCGGTG AGGATCGATG 
     4801 ACCTGGACCA CCTCCCGGAG ACCACCACCA TCGACGCCTC CTCCATTGGC ATCGTCCAGC 
     4861 CTGAGCTGAC TCTGGAGCAG GAGGATTTGG CCGAAGGGAA GCACGGGAAA GCTGCCAAGC 
     4921 GAAGTCACAA GAGAAAGCAG AAGCCAGAAG AGGAGGCGGG TGCTCCGGTG CCCGAGGACG 
     4981 CCACCTTCAG CGAATACTCA GAGAAAGAGA CGGAGTTCAC AGGCAGTGTA GGCGACGAGA 
     5041 CCAATTCCGC AGTACAGAGC ATTCAGCAGG TAGTGGTGAC CCTGGGTGAC CCAAATGTGA 
     5101 CCACACCATC GAGCTCAGTC GGCTTAACCA ACATCACCGT GACCCCCATC ACCACTGCGG 
     5161 CCGCGACTCA GTTTACCAAT CTCCAGCCGG TGGCCGTGGG GCACCTTACC ACCCCTGAAC 
     5221 GCCAGTTACA GCTGGACAAC TCAATCCTGA CCGTGACCTT TGATACCGTC AGCGGCTCTG 
     5281 CCATGTTGCA CAACCGCCAA AATGACGTCC AGATCCACCC CCAGCCGGAA GCCTCGAACC 
     5341 CACAGTCTGT GGCCCATTTC ATCAACCTGA CGACCCTGGT CAACTCCATC ACGCCCCTGG 
     5401 GGAGCCAGCT TAGTGACCAG CACCCGCTCA CGTGGCGGGC AGTGCCCCAG ACTGACGTCT 
     5461 TGCCACCCTC GCAGCCGCAG GCACCCCCAC AGCAGGCGGC CCAGCCCCAG GTGCAGGCGG 
     5521 AGCAGCAGCA GCAGCAGATG TACAGCTACA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     5581 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5641 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5701 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5761 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5821 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5881 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5941 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     6001 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     6061 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     6121 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     6181 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     6241 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     6301 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     6361 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     6421 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     6481 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     6541 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6601 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6661 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6721 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6781 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6841 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6901 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6961 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     7021 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     7081 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     7141 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     7201 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     7261 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     7321 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     7381 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     7441 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     7501 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     7561 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7621 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7681 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7741 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7801 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7861 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7921 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7981 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     8041 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     8101 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     8161 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     8221 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     8281 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     8341 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     8401 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     8461 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     8521 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     8581 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8641 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8701 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8761 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8821 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8881 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8941 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     9001 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     9061 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     9121 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     9181 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     9241 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     9301 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     9361 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//