LOCUS       RC222491                7608 bp    DNA     circular  SYN 
DEFINITION  TMC2 (Myc-DDK-tagged)-Human transmembrane channel-like 2 (TMC2)
ACCESSION   NM_080751
VERSION     NM_080751.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3763..3854 
                     /label="Myc-DDK"  
     misc_feature    3884..4469 
                     /label="PolyA signal"  
     misc_feature    3941..3960 
                     /label="Restriction Enzyme"  
     misc_feature    4745..5417 
                     /label="ColE1"  
     misc_feature    5847..6641 
                     /label="Kan/Neo"  
     misc_feature    6676..6978 
                     /label="SV40 ori"  
     misc_feature    7064..7541 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3738..3745 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3738 
                     /label="TMC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCCACCAG GTAAAGGGCC TGAAAGAGGA AGCACGAGGC GGAGTGAAAG 
     1081 GGCGGGTGAA GAGCGGCTCT CCACACACAG GTGACAGGCT GGGAAGGAGA TCCTCAAGCA 
     1141 AGCGGGCTCT CAAAGCCGAG GGGACCCCAG GCAGGCGCGG AGCTCAGCGA AGCCAGAAGG 
     1201 AGCGCGCCGG GGGCAGCCCA AGCCCGGGGT CTCCCCGGAG GAAGCAAACA GGGCGCAGGA 
     1261 GACACAGAGA AGAGCTGGGG GAGCAGGAGC GGGGCGAGGC AGAGAGGACC TGCGAGGGCA 
     1321 GGAGAAAGCG CGACGAGAGG GCCTCCTTCC AGGAGCGGAC AGCAGCCCCA AAGAGGGAAA 
     1381 AGGAGATTCC GAGGAGGGAG GAGAAGTCGA AGCGGCAGAA GAAACCCAGG TCATCCTCCT 
     1441 TGGCCTCCAG TGCCTCTGGT GGGGAGTCCC TGTCCGAGGA GGAACTGGCC CAGATCCTGG 
     1501 AGCAGGTGGA AGAAAAAAAG AAGCTCATTG CCACCATGCG GAGCAAGCCC TGGCCCATGG 
     1561 CGAAGAAGCT GACAGAGCTC AGGGAGGCCC AGGAATTTGT GGAGAAGTAT GAAGGTGCCT 
     1621 TGGGAAAGGG GAAAGGCAAG CAACTATATG CCTACAAGAT GCTGATGGCC AAGAAATGGG 
     1681 TCAAATTTAA GAGAGACTTT GATAATTTCA AGACTCAATG TATCCCCTGG GAAATGAAGA 
     1741 TCAAGGACAT TGAAAGTCAC TTTGGTTCTT CAGTGGCATC GTATTTCATC TTTCTCCGAT 
     1801 GGATGTATGG AGTTAACCTT GTCCTTTTTG GCTTAATATT TGGTCTAGTC ATAATCCCAG 
     1861 AGGTACTGAT GGGCATGCCC TATGGGAGTA TTCCCAGAAA GACAGTGCCT CGGGCTGAGG 
     1921 AAGAAAAGGC CATGGATTTT TCTGTCCTTT GGGATTTTGA GGGCTATATC AAGTACTCTG 
     1981 CACTCTTCTA TGGCTACTAC AACAACCAGA GGACCATCGG GTGGCTGAGG TACCGGCTGC 
     2041 CTATGGCTTA CTTTATGGTG GGGGTCAGCG TGTTCGGCTA CAGCCTGATT ATTGTCATTC 
     2101 GATCGATGGC CAGCAATACC CAAGGAAGCA CAGGCGAAGG GGAGAGTGAC AACTTCACAT 
     2161 TCAGCTTCAA GATGTTCACC AGCTGGGACT ACCTGATCGG GAATTCAGAG ACAGCTGATA 
     2221 ACAAATATGC ATCCATCACC ACCAGCTTCA AGGAATCAAT AGTGGATGAA CAAGAGAGTA 
     2281 ACAAAGAAGA AAATATCCAT CTGACAAGAT TTCTTCGTGT CCTGGCCAAC TTTCTCATCA 
     2341 TCTGCTGTTT GTGTGGAAGT GGGTACCTCA TTTACTTTGT GGTTAAGCGA TCTCAGCAAT 
     2401 TCTCCAAAAT GCAGAATGTC AGCTGGTATG AAAGGAATGA GGTAGAGATC GTGATGTCCC 
     2461 TGCTTGGAAT GTTTTGTCCC CCTCTGTTTG AAACCATCGC TGCCCTGGAG AATTACCACC 
     2521 CACGCACTGG ACTGAAGTGG CAGCTGGGAC GCATCTTTGC ACTCTTCCTG GGGAACCTCT 
     2581 ACACATTTCT CTTGGCCCTG ATGGATGACG TCCACCTCAA GCTTGCTAAT GAAGAGACAA 
     2641 TAAAGAACAT CACTCACTGG ACTCTGTTTA ACTATTACAA CTCTTCTGGT TGGAACGAGA 
     2701 GTGTCCCCCG ACCACCCCTG CACCCTGCAG ATGTGCCCCG GGGTTCTTGC TGGGAGACAG 
     2761 CTGTGGGCAT TGAATTCATG AGGCTGACGG TGTCTGACAT GCTGGTAACG TACATCACCA 
     2821 TCCTGCTGGG GGACTTCCTA CGGGCTTGTT TTGTGCGGTT CATGAACTAC TGCTGGTGCT 
     2881 GGGACTTGGA GGCTGGATTT CCTTCATATG CTGAGTTTGA TATTAGTGGA AATGTGCTGG 
     2941 GTTTGATCTT CAACCAAGGA ATGATCTGGA TGGGCTCCTT CTATGCTCCA GGCCTGGTGG 
     3001 GCATTAATGT GCTGCGCCTG CTGACCTCCA TGTACTTCCA GTGCTGGGCG GTGATGAGCA 
     3061 GCAACGTACC CCATGAACGC GTGTTCAAAG CCTCCCGATC CAACAACTTC TACATGGGCC 
     3121 TCCTGCTGCT GGTGCTCTTC CTCAGCCTCC TGCCGGTGGC CTACACCATC ATGTCCCTCC 
     3181 CACCCTCCTT TGACTGCGGG CCGTTCAGTG GGAAAAACAG AATGTACGAT GTCCTCCAAG 
     3241 AGACCATTGA AAACGATTTC CCAACCTTCC TGGGCAAGAT CTTTGCTTTC CTCGCCAATC 
     3301 CAGGCCTGAT CATCCCAGCC ATCCTGCTGA TGTTCTTGGC CATTTACTAC CTGAACTCAG 
     3361 TTTCCAAAAG CCTTTCCCGA GCTAATGCCC AGCTGAGGAA GAAAATCCAA GTGCTCCGTG 
     3421 AAGTTGAGAA GAGTCACAAA TCTGTAAAAG GCAAAGCCAC AGCCAGAGAT TCAGAGGACA 
     3481 CACCTAAAAG CAGCTCCAAA AATGCCACCC AGCTCCAACT CACCAAGGAA GAGACCACTC 
     3541 CTCCCTCTGC CAGCCAAAGC CAGGCCATGG ACAAGAAGGC GCAGGGCCCT GGGACCTCCA 
     3601 ATTCTGCCAG CAGGACCACA CTGCCTGCCT CTGGACACCT TCCTATATCT CGGCCCCCTG 
     3661 GAATCGGACC AGATTCTGGC CACGCCCCAT CTCAGACTCA TCCGTGGAGG TCAGCCTCTG 
     3721 GAAAGAGTGC TCAGAGACCT CCCCACAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     3781 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3841 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3901 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3961 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4021 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4081 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4141 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4201 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4261 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4321 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4381 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4441 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4501 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4561 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4621 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4681 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4741 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4801 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4861 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4921 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4981 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5041 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5101 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5161 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5221 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5281 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5341 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5401 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5461 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5521 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5581 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5641 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5701 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5761 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5821 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5881 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5941 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6001 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6061 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6121 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6181 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6241 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6301 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6361 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6421 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6481 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6541 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6601 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6661 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6721 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6781 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6841 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6901 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6961 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7021 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7081 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7141 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7201 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7261 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7321 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7381 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7441 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7501 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7561 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//