LOCUS       RC222401                8292 bp    DNA     circular  SYN 
DEFINITION  SLC4A5 (Myc-DDK-tagged)-Human solute carrier family 4, sodium bicarbonate cotransporter, member 5 (SLC4A5), transcript variant a
ACCESSION   NM_021196
VERSION     NM_021196.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4449..4540 
                     /label="Myc-DDK"  
     misc_feature    4570..5155 
                     /label="PolyA signal"  
     misc_feature    4627..4646 
                     /label="Restriction Enzyme"  
     misc_feature    5431..6103 
                     /label="ColE1"  
     misc_feature    6533..7327 
                     /label="Kan/Neo"  
     misc_feature    7362..7664 
                     /label="SV40 ori"  
     misc_feature    7750..8227 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4431..4437 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4431 
                     /label="SLC4A5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGTGAAG GAGGAGAAGG CTGGGGTAGG AAAGCTGGAC CACACTAACC 
     1081 ACAGGAGGAG ATTTCCGGAT CAGAAAGAAT GCCCTCCTAT CCACATTGGG CTTCCAGTAC 
     1141 CCACTTACCC TCAAAGAAAA ACTGACCAGA AGGGACATCT TTCAGGCCTG CAAAAAGTCC 
     1201 ACTGGGGCCT GCGGCCAGAC CAGCCACAGC AGGAACTGAC TGGCCCAGGG AGTGGGGCAA 
     1261 GCAGCCAGGA CAGCAGCATG GATCTTATCA GCAGGACTCG GTCCCCAGCT GCTGAGCAGC 
     1321 TCCAGGACAT CCTGGGGGAG GAAGATGAGG CTCCCAACCC CACCCTCTTT ACAGAGATGG 
     1381 ATACTCTGCA GCATGACGGA GACCAGATGG AGTGGAAGGA GTCAGCCAGG TGGATAAAGT 
     1441 TTGAAGAAAA GGTAGAGGAA GGCGGCGAAC GCTGGAGCAA GCCCCACGTG TCCACACTAT 
     1501 CCCTGCACAG CCTCTTCGAG CTCCGTACCT GCCTGCAGAC GGGGACGGTG CTGCTGGATT 
     1561 TGGACAGTGG CTCCTTACCA CAGATCATAG ATGATGTCAT TGAGAAGCAG ATTGAGGATG 
     1621 GTCTCCTGCG GCCAGAGCTC CGGGAGAGGG TCAGTTACGT CCTCCTGAGG AGGCACCGCC 
     1681 ACCAAACCAA GAAGCCCATC CACCGCTCCT TAGCTGACAT TGGGAAGTCA GTCTCCACCA 
     1741 CAAATCGCAG TCCTGCCCGG AGCCCTGGTG CTGGCCCGAG TCTACACCAC TCCACGGAAG 
     1801 ACCTGCGGAT GCGGCAGAGT GCAAATTACG GACGTCTGTG TCATGCCCAG AGCAGAAGCA 
     1861 TGAATGACAT TTCTCTCACC CCAAACACAG ACCAGCGGAA AAACAAATTC ATGAAGAAGA 
     1921 TCCCCAAGGA CTCAGAAGCG TCCAACGTGC TCGTGGGCGA GGTGGACTTC CTAGACCAGC 
     1981 CATTCATCGC GTTCGTGCGC CTCATCCAGT CGGCCATGCT GGGAGGAGTG ACCGAGGTGC 
     2041 CTGTCCCCAC CAGATTTCTG TTTATACTAC TGGGACCTTC TGGGAGAGCA AAATCCTACA 
     2101 ATGAAATTGG CCGTGCCATT GCAACCCTCA TGGTAGATGA TCTCTTCAGT GACGTGGCCT 
     2161 ACAAAGCCCG CAATCGGGAA GATCTGATCG CAGGAATTGA TGAATTTCTG GATGAGGTCA 
     2221 TCGTCCTTCC TCCTGGAGAA TGGGACCCAA ATATCCGGAT TGAGCCCCCC AAGAAGGTGC 
     2281 CCTCTGCTGA CAAGAGGAAA TCTGTGTTCT CCCTAGCAGA GCTGGGCCAG ATGAATGGCT 
     2341 CTGTGGGAGG AGGCGGCGGA GCTCCTGGAG GAGGCAATGG AGGTGGTGGT GGTGGTGGCA 
     2401 GTGGCGGCGG GGCTGGCAGT GGCGGGGCCG GCGGAACAAG CAGCGGGGAT GATGGAGAGA 
     2461 TGCCAGCCAT GCATGAAATC GGGGAGGAAC TTATCTGGAC AGGAAGGTTC TTCGGTGGCC 
     2521 TGTGTCTGGA TATCAAGAGG AAGTTGCCCT GGTTCCCAAG TGACTTCTAT GATGGCTTCC 
     2581 ACATTCAGTC CATCTCTGCC ATCCTATTCA TCTACCTCGG CTGTATCACC AACGCGATCA 
     2641 CCTTTGGTGG GCTTCTGGGG GATGCCACCG ACAATTATCA GGGAGTGATG GAGAGCTTCC 
     2701 TGGGCACTGC CATGGCTGGC TCCTTGTTCT GCCTCTTCTC GGGACAGCCT CTCATCATTC 
     2761 TCAGCAGCAC GGGGCCCATC CTCATCTTTG AGAAGCTCCT CTTCGACTTC AGCAAAGGCA 
     2821 ATGGCCTGGA CTACATGGAG TTCCGCCTCT GGATTGGCCT ACACTCAGCT GTCCAGTGCC 
     2881 TTATCCTAGT GGCCACAGAT GCCAGCTTTA TCATCAAATA TATCACCCGC TTCACCGAGG 
     2941 AGGGCTTCTC CACCCTTATC AGCTTCATCT TCATCTACGA TGCCATCAAG AAGATGATCG 
     3001 GTGCCTTCAA GTACTACCCT ATCAATATGG ACTTCAAGCC AAACTTCATC ACTACCTACA 
     3061 AGTGCGAGTG TGTCGCCCCT GACACAGTGA ATACAACCGT GTTCAATGCT TCAGCCCCAT 
     3121 TGGCACCAGA CACCAACGCT TCTCTGTACA ACCTCCTTAA CCTCACAGCG TTGGACTGGT 
     3181 CCCTGCTGAG CAAGAAGGAG TGTCTGAGCT ACGGCGGGCG CCTGCTTGGG AATTCCTGCA 
     3241 AGTTTATCCC AGACCTGGCG CTCATGTCCT TCATCCTTTT CTTTGGGACA TACTCCATGA 
     3301 CCCTGACCCT GAAGAAGTTC AAATTCAGCC GCTATTTTCC TACCAAGGTC CGGGCCCTGG 
     3361 TGGCTGACTT TTCCATTGTT TTCTCCATCC TGATGTTCTG TGGAATCGAT GCCTGTTTTG 
     3421 GCCTAGAAAC TCCCAAGCTG CATGTGCCCA GTGTCATCAA GCCACCGCGG CCTGACCGAG 
     3481 GCTGGTTCGT GGCCCCCTTT GGGAAGAACC CGTGGTGGGT ATACCCAGCA AGCATCCTGC 
     3541 CCGCCCTGCT GGTGACCATC CTGATCTTCA TGGACCAGCA GATCACTGCC GTCATTGTCA 
     3601 ACCGGAAGGA GAACAAACTG AAGAAGGCTG CCGGCTACCA TCTGGACCTG TTCTGGGTGG 
     3661 GCATCCTCAT GGCTTTGTGC TCCTTTATGG GGCTCCCCTG GTACGTGGCT GCCACGGTCA 
     3721 TCTCCATCGC CCACATCGAC AGCCTCAAGA TGGAGACAGA GACCAGTGCC CCTGGGGAGC 
     3781 AGCCCCAGTT TCTGGGAGTC AGGGAACAGA GAGTAACCGG CATCATCGTC TTCATCCTGA 
     3841 CGGGAATCTC TGTCTTCCTG GCTCCCATCC TAAAGTGTAT CCCCCTGCCG GTGCTGTACG 
     3901 GAGTCTTCCT CTACATGGGC GTGGCCTCCC TGAATGGCAT CCAGATGGGG ACCGGAGGGT 
     3961 CAGAATTCAA AATCCAGAAG AAGCTGACTC CATTCTGGGA ACGCTGCAAG CTCTTCCTGA 
     4021 TGCCAGCCAA GCACCAGCCG GACCATGCCT TCCTGCGGCA CGTGCCGCTG CGCCGGATCC 
     4081 ACCTCTTCAC CCTGGTGCAG ATCCTCTGCC TGGCGGTGCT CTGGATCCTC AAATCCACGG 
     4141 TGGCTGCCAT CATCTTCCCG GTCATGATCC TGGGCCTCAT CATCGTTCGA AGGCTTCTGG 
     4201 ATTTCATCTT TTCCCAGCAC GACCTGGCCT GGATTGACAA CATCCTCCCA GAGAAGGAAA 
     4261 AAAAGGAGAC AGACAAGAAG AGGAAGAGAA AAAAAGGGGC CCACGAGGAC TGTGATGAGG 
     4321 AGCCCCAGTT CCCTCCTCCC TCGGTTATAA AGATTCCCAT GGAAAGTGTC CAATCAGATC 
     4381 CCCAAAACGG TATCCACTGC ATTGCCAGAA AAAGATCTTC CAGTTGGAGT TACTCACTCA 
     4441 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4501 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4561 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4621 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4681 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4741 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4801 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4861 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4921 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4981 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5041 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5101 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5161 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5221 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5281 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5341 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5401 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5461 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5521 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5581 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5641 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5701 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5761 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5821 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5881 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5941 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6001 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6061 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6121 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6181 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6241 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6301 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6361 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6421 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6481 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6541 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6601 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6661 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6721 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6781 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6841 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6901 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6961 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7021 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7081 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7141 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7201 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7261 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7321 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7381 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7441 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7501 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7561 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7741 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7801 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7861 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7921 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7981 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8041 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8101 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8161 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8221 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8281 AACGCGAATT TT
//