LOCUS       RC222384                9183 bp    DNA     circular  SYN 
DEFINITION  NOS1 (Myc-DDK-tagged)-Human nitric oxide synthase 1 (neuronal) (NOS1), transcript variant 1
ACCESSION   NM_000620
VERSION     NM_000620.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5340..5431 
                     /label="Myc-DDK"  
     misc_feature    5461..6046 
                     /label="PolyA signal"  
     misc_feature    5518..5537 
                     /label="Restriction Enzyme"  
     misc_feature    6322..6994 
                     /label="ColE1"  
     misc_feature    7424..8218 
                     /label="Kan/Neo"  
     misc_feature    8253..8555 
                     /label="SV40 ori"  
     misc_feature    8641..9118 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5322..5328 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5322 
                     /label="NOS1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGATCAC ATGTTCGGTG TTCAGCAAAT CCAGCCCAAT GTCATTTCTG 
     1081 TTCGTCTCTT CAAGCGCAAA GTTGGGGGCC TGGGATTTCT GGTGAAGGAG CGGGTCAGTA 
     1141 AGCCGCCCGT GATCATCTCT GACCTGATTC GTGGGGGCGC CGCAGAGCAG AGTGGCCTCA 
     1201 TCCAGGCCGG AGACATCATT CTTGCGGTCA ACGGCCGGCC CTTGGTGGAC CTGAGCTATG 
     1261 ACAGCGCCCT GGAGGTACTC AGAGGCATTG CCTCTGAGAC CCACGTGGTC CTCATTCTGA 
     1321 GGGGCCCTGA AGGTTTCACC ACGCACCTGG AGACCACCTT TACAGGTGAT GGGACCCCCA 
     1381 AGACCATCCG GGTGACACAG CCCCTGGGTC CCCCCACCAA AGCCGTGGAT CTGTCCCACC 
     1441 AGCCACCGGC CGGCAAAGAA CAGCCCCTGG CAGTGGATGG GGCCTCGGGT CCCGGGAATG 
     1501 GGCCTCAGCA TGCCTACGAT GATGGGCAGG AGGCTGGCTC ACTCCCCCAT GCCAACGGCC 
     1561 TGGCCCCCAG GCCCCCAGGC CAGGACCCCG CGAAGAAAGC AACCAGAGTC AGCCTCCAAG 
     1621 GCAGAGGGGA GAACAATGAA CTGCTCAAGG AGATAGAGCC TGTGCTGAGC CTTCTCACCA 
     1681 GTGGGAGCAG AGGGGTCAAG GGAGGGGCAC CTGCCAAGGC AGAGATGAAA GATATGGGAA 
     1741 TCCAGGTGGA CAGAGATTTG GACGGCAAGT CACACAAACC TCTGCCCCTC GGCGTGGAGA 
     1801 ACGACCGAGT CTTCAATGAC CTATGGGGGA AGGGCAATGT GCCTGTCGTC CTCAACAACC 
     1861 CATATTCAGA GAAGGAGCAG CCCCCCACCT CAGGAAAACA GTCCCCCACA AAGAATGGCA 
     1921 GCCCCTCCAA GTGTCCACGC TTCCTCAAGG TCAAGAACTG GGAGACTGAG GTGGTTCTCA 
     1981 CTGACACCCT CCACCTTAAG AGCACATTGG AAACGGGATG CACTGAGTAC ATCTGCATGG 
     2041 GCTCCATCAT GCATCCTTCT CAGCATGCAA GGAGGCCTGA AGACGTCCGC ACAAAAGGAC 
     2101 AGCTCTTCCC TCTCGCCAAA GAGTTTATTG ATCAATACTA TTCATCAATT AAAAGATTTG 
     2161 GCTCCAAAGC CCACATGGAA AGGCTGGAAG AGGTGAACAA AGAGATCGAC ACCACTAGCA 
     2221 CTTACCAGCT CAAGGACACA GAGCTCATCT ATGGGGCCAA GCACGCCTGG CGGAATGCCT 
     2281 CGCGCTGTGT GGGCAGGATC CAGTGGTCCA AGCTGCAGGT ATTCGATGCC CGTGACTGCA 
     2341 CCACGGCCCA CGGGATGTTC AACTACATCT GTAACCATGT CAAGTATGCC ACCAACAAAG 
     2401 GGAACCTCAG GTCTGCCATC ACCATATTCC CCCAGAGGAC AGACGGCAAG CACGACTTCC 
     2461 GAGTCTGGAA CTCCCAGCTC ATCCGCTACG CTGGCTACAA GCAGCCTGAC GGCTCCACCC 
     2521 TGGGGGACCC AGCCAATGTG CAGTTCACAG AGATATGCAT ACAGCAGGGC TGGAAACCGC 
     2581 CTAGAGGCCG CTTCGATGTC CTGCCGCTCC TGCTTCAGGC CAACGGCAAT GACCCTGAGC 
     2641 TCTTCCAGAT TCCTCCAGAG CTGGTGTTGG AAGTTCCCAT CAGGCACCCC AAGTTTGAGT 
     2701 GGTTCAAGGA CCTGGGGCTG AAGTGGTACG GCCTCCCCGC CGTGTCCAAC ATGCTCCTAG 
     2761 AGATTGGCGG CCTGGAGTTC AGCGCCTGTC CCTTCAGTGG CTGGTACATG GGCACAGAGA 
     2821 TTGGTGTCCG CGACTACTGT GACAACTCCC GCTACAATAT CCTGGAGGAA GTGGCCAAGA 
     2881 AGATGAACTT AGACATGAGG AAGACGTCCT CCCTGTGGAA GGACCAGGCG CTGGTGGAGA 
     2941 TCAATATCGC GGTTCTCTAT AGCTTCCAGA GTGACAAAGT GACCATTGTT GACCATCACT 
     3001 CCGCCACCGA GTCCTTCATT AAGCACATGG AGAATGAGTA CCGCTGCCGG GGGGGCTGCC 
     3061 CTGCCGACTG GGTGTGGATC GTGCCCCCCA TGTCCGGAAG CATCACCCCT GTGTTCCACC 
     3121 AGGAGATGCT CAACTACCGG CTCACCCCCT CCTTCGAATA CCAGCCTGAT CCCTGGAACA 
     3181 CGCATGTCTG GAAAGGCACC AACGGGACCC CCACAAAGCG GCGAGCCATC GGCTTCAAGA 
     3241 AGCTAGCAGA AGCTGTCAAG TTCTCGGCCA AGCTGATGGG GCAGGCTATG GCCAAGAGGG 
     3301 TGAAAGCGAC CATCCTCTAT GCCACAGAGA CAGGCAAATC GCAAGCTTAT GCCAAGACCT 
     3361 TGTGTGAGAT CTTCAAACAC GCCTTTGATG CCAAGGTGAT GTCCATGGAA GAATATGACA 
     3421 TTGTGCACCT GGAACATGAA ACTCTGGTCC TTGTGGTCAC CAGCACCTTT GGCAATGGAG 
     3481 ATCCCCCTGA GAATGGGGAG AAATTCGGCT GTGCTTTGAT GGAAATGAGG CACCCCAACT 
     3541 CTGTGCAGGA AGAAAGGAAG AGCTACAAGG TCCGATTCAA CAGCGTCTCC TCCTACTCTG 
     3601 ACTCCCAAAA ATCATCAGGC GATGGGCCCG ACCTCAGAGA CAACTTTGAG AGTGCTGGAC 
     3661 CCCTGGCCAA TGTGAGGTTC TCAGTTTTTG GCCTCGGCTC ACGAGCATAC CCTCACTTTT 
     3721 GCGCCTTCGG ACACGCTGTG GACACCCTCC TGGAAGAACT GGGAGGGGAG AGGATCTTGA 
     3781 AGATGAGGGA AGGGGATGAG CTCTGTGGGC AGGAAGAGGC TTTCAGGACC TGGGCCAAGA 
     3841 AGGTCTTCAA GGCAGCCTGT GATGTCTTCT GTGTGGGAGA TGATGTCAAC ATTGAAAAGG 
     3901 CCAACAATTC CCTCATCAGC AATGATCGCA GCTGGAAGAG AAACAAGTTC CGCCTCACCT 
     3961 TTGTGGCCGA AGCTCCAGAA CTCACACAAG GTCTATCCAA TGTCCACAAA AAGCGAGTCT 
     4021 CAGCTGCCCG GCTCCTTAGC CGTCAAAACC TCCAGAGCCC TAAATCCAGT CGGTCAACTA 
     4081 TCTTCGTGCG TCTCCACACC AACGGGAGCC AGGAGCTGCA GTACCAGCCT GGGGACCACC 
     4141 TGGGTGTCTT CCCTGGCAAC CACGAGGACC TCGTGAATGC CCTGATCGAG CGGCTGGAGG 
     4201 ACGCGCCGCC TGTCAACCAG ATGGTGAAAG TGGAACTGCT GGAGGAGCGG AACACGGCTT 
     4261 TAGGTGTCAT CAGTAACTGG ACAGACGAGC TCCGCCTCCC GCCCTGCACC ATCTTCCAGG 
     4321 CCTTCAAGTA CTACCTGGAC ATCACCACGC CACCAACGCC TCTGCAGCTG CAGCAGTTTG 
     4381 CCTCCCTAGC TACCAGCGAG AAGGAGAAGC AGCGTCTGCT GGTCCTCAGC AAGGGTTTGC 
     4441 AGGAGTACGA GGAATGGAAA TGGGGCAAGA ACCCCACCAT CGTGGAGGTG CTGGAGGAGT 
     4501 TCCCATCTAT CCAGATGCCG GCCACCCTGC TCCTGACCCA GCTGTCCCTG CTGCAGCCCC 
     4561 GCTACTATTC CATCAGCTCC TCCCCAGACA TGTACCCTGA TGAAGTGCAC CTCACTGTGG 
     4621 CCATCGTTTC CTACCGCACT CGAGATGGAG AAGGACCAAT TCACCACGGC GTATGCTCCT 
     4681 CCTGGCTCAA CCGGATACAG GCTGACGAAC TGGTCCCCTG TTTCGTGAGA GGAGCACCCA 
     4741 GCTTCCACCT GCCCCGGAAC CCCCAAGTCC CCTGCATCCT CGTTGGACCA GGCACCGGCA 
     4801 TTGCCCCTTT CCGAAGCTTC TGGCAACAGC GGCAATTTGA TATCCAACAC AAAGGAATGA 
     4861 ACCCCTGCCC CATGGTCCTG GTCTTCGGGT GCCGGCAATC CAAGATAGAT CATATCTACA 
     4921 GGGAAGAGAC CCTGCAGGCC AAGAACAAGG GGGTCTTCAG AGAGCTGTAC ACGGCTTACT 
     4981 CCCGGGAGCC AGACAAACCA AAGAAGTACG TGCAGGACAT CCTGCAGGAG CAGCTGGCGG 
     5041 AGTCTGTGTA CCGAGCCCTG AAGGAGCAAG GGGGCCACAT ATACGTCTGT GGGGACGTCA 
     5101 CCATGGCTGC TGATGTCCTC AAAGCCATCC AGCGCATCAT GACCCAGCAG GGGAAGCTCT 
     5161 CGGCAGAGGA CGCCGGCGTA TTCATCAGCC GGATGAGGGA TGACAACCGA TACCATGAGG 
     5221 ATATTTTTGG AGTCACCCTG CGAACGTACG AAGTGACCAA CCGCCTTAGA TCTGAGTCCA 
     5281 TTGCCTTCAT TGAAGAGAGC AAAAAAGACA CCGATGAGGT TTTCAGCTCC ACGCGTACGC 
     5341 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5401 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5461 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5521 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5581 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5641 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5701 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5761 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5821 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5881 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5941 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6001 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6061 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6121 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6181 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6241 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6301 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6361 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6421 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6481 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6541 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6601 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6661 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6721 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6781 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6841 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6901 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6961 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7021 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7081 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7141 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7201 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7261 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7321 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7381 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7441 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7501 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7561 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7621 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7681 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7741 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7801 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7861 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7921 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7981 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8041 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8101 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8161 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8221 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8281 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8341 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8401 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8461 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8521 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8581 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8641 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8701 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8761 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8821 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8881 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8941 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9001 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9061 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9121 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9181 TTT
//