LOCUS       RC222379                9246 bp    DNA     circular  SYN 
DEFINITION  MAGI2 (Myc-DDK-tagged)-Human membrane associated guanylate kinase, WW and PDZ domain containing 2 (MAGI2)
ACCESSION   NM_012301
VERSION     NM_012301.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5403..5494 
                     /label="Myc-DDK"  
     misc_feature    5524..6109 
                     /label="PolyA signal"  
     misc_feature    5581..5600 
                     /label="Restriction Enzyme"  
     misc_feature    6385..7057 
                     /label="ColE1"  
     misc_feature    7487..8281 
                     /label="Kan/Neo"  
     misc_feature    8316..8618 
                     /label="SV40 ori"  
     misc_feature    8704..9181 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5385..5391 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5385 
                     /label="MAGI2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCAAAAGC TTGAAAAAGA AAAGCCACTG GACTAGCAAA GTCCATGAGA 
     1081 GTGTCATTGG CAGGAACCCG GAGGGCCAGC TGGGCTTTGA ACTGAAGGGG GGCGCCGAGA 
     1141 ATGGACAGTT CCCCTACCTG GGGGAGGTGA AGCCCGGCAA GGTGGCCTAT GAGAGCGGCA 
     1201 GCAAATTGGT GTCGGAGGAG CTGCTGCTGG AGGTGAACGA GACCCCCGTG GCGGGGCTCA 
     1261 CCATCAGGGA CGTGCTGGCC GTGATCAAAC ACTGCAAGGA CCCCCTCCGG CTCAAGTGTG 
     1321 TCAAGCAAGG AGGAATTGTT GATAAAGACC TTCGTCACTA CCTCAACTTA CGATTTCAAA 
     1381 AGGGTTCTGT GGACCATGAG CTTCAGCAAA TCATTCGTGA CAACCTCTAC CTCCGCACGG 
     1441 TGCCATGCAC CACAAGGCCA CATAAGGAGG GTGAGGTCCC TGGAGTGGAT TATATTTTCA 
     1501 TCACTGTTGA AGATTTTATG GAATTGGAGA AAAGTGGTGC TCTCCTAGAA AGTGGGACTT 
     1561 ATGAAGACAA TTACTACGGT ACCCCAAAGC CGCCAGCAGA ACCAGCACCA TTATTGTTAA 
     1621 ATGTAACAGA CCAGATACTT CCAGGAGCCA CTCCAAGTGC TGAAGGAAAA CGGAAGAGGA 
     1681 ATAAATCAGT GAGCAACATG GAGAAAGCCA GTATAGAGCC TCCTGAGGAG GAAGAGGAAG 
     1741 AGAGGCCTGT GGTCAATGGA AATGGAGTAG TAGTAACACC AGAATCCAGT GAACATGAAG 
     1801 ACAAAAGTGC AGGTGCCTCA GGGGAGATGC CCTCCCAGCC TTATCCTGCA CCAGTGTACA 
     1861 GTCAGCCTGA GGAGCTGAAG GAGCAGATGG ATGACACAAA GCCAACTAAA CCTGAAGACA 
     1921 ATGAGGAACC AGACCCATTG CCTGATAACT GGGAAATGGC CTATACAGAG AAGGGCGAAG 
     1981 TCTACTTCAT TGACCATAAC ACAAAGACAA CATCATGGCT GGATCCACGA CTTGCGAAAA 
     2041 AGGCTAAACC TCCAGAAGAG TGCAAAGAAA ATGAGCTTCC ATATGGCTGG GAAAAAATCG 
     2101 ATGATCCCAT TTATGGCACT TATTATGTTG ACCACATAAA TAGAAGAACA CAGTTTGAAA 
     2161 ATCCTGTCCT GGAAGCAAAA AGGAAGCTAC AGCAACATAA CATGCCCCAC ACAGAACTTG 
     2221 GAACAAAGCC CCTGCAGGCC CCAGGTTTCC GAGAAAAACC ACTCTTCACC CGGGATGCAT 
     2281 CCCAGTTGAA GGGAACATTC CTCAGCACCA CCCTAAAAAA GAGCAACATG GGCTTTGGAT 
     2341 TTACCATCAT TGGTGGAGAC GAGCCTGATG AGTTTCTGCA GGTGAAAAGT GTGATTCCGG 
     2401 ATGGGCCTGC AGCACAGGAT GGAAAAATGG AAACAGGTGA TGTCATTGTC TATATTAATG 
     2461 AAGTTTGTGT CCTTGGACAC ACTCATGCAG ATGTTGTCAA ACTTTTCCAG TCTGTTCCTA 
     2521 TTGGTCAGAG TGTCAACCTG GTGTTGTGTC GTGGCTACCC TTTGCCCTTT GATCCTGAAG 
     2581 ACCCTGCTAA CAGCATGGTG CCACCCCTTG CAATAATGGA GAGGCCACCT CCAGTGATGG 
     2641 TCAATGGAAG ACACAACTAT GAAACATATT TGGAGTACAT TTCTCGGACC TCACAGTCAG 
     2701 TTCCAGATAT AACAGATCGG CCGCCTCATT CTCTGCACTC CATGCCAACT GATGGTCAGC 
     2761 TAGACGGCAC GTATCCACCG CCCGTCCATG ATGACAATGT GTCTATGGCT TCATCTGGGG 
     2821 CCACCCAAGC TGAACTTATG ACCTTAACCA TTGTGAAAGG TGCCCAGGGC TTCGGCTTCA 
     2881 CTATTGCCGA CAGTCCTACA GGACAGCGGG TGAAACAAAT ACTTGACATT CAGGGATGCC 
     2941 CTGGCCTGTG TGAAGGCGAC CTCATTGTTG AGATCAACCA GCAGAATGTA CAGAACCTGA 
     3001 GCCATACAGA AGTAGTGGAT ATACTTAAGG ACTGTCCCAT TGGAAGTGAA ACTTCTTTGA 
     3061 TTATCCATCG AGGAGGTTTC TTTTCTCCAT GGAAAACTCC AAAGCCTATA ATGGACCGAT 
     3121 GGGAGAATCA AGGCAGTCCT CAAACGAGTT TATCTGCTCC GGCCATACCG CAGAACCTGC 
     3181 CCTTCCCACC TGCCCTTCAC AGGAGCTCCT TTCCTGACTC AACAGAGGCC TTTGACCCAC 
     3241 GGAAGCCTGA TCCATATGAG CTCTACGAGA AATCTAGGGC CATTTATGAA AGTAGGCAAC 
     3301 AAGTGCCACC CAGGACCAGT TTTCGAATGG ATTCCTCTGG TCCAGATTAT AAGGAATTGG 
     3361 ATGTTCATCT TCGGAGGATG GAGTCTGGAT TTGGCTTCAG AATCCTCGGG GGAGATGAGC 
     3421 CTGGACAGCC TATTTTGATT GGAGCTGTCA TTGCCATGGG CTCAGCCGAC AGAGATGGCC 
     3481 GCCTTCACCC AGGAGATGAG CTTGTGTATG TTGATGGGAT TCCAGTAGCC GGCAAAACCC 
     3541 ACCGCTATGT CATCGACCTC ATGCACCACG CAGCCCGCAA TGGGCAGGTC AACCTCACTG 
     3601 TGAGAAGAAA GGTGCTATGT GGAGGGGAGC CCTGCCCAGA GAACGGGAGA AGTCCAGGCT 
     3661 CTGTATCCAC CCACCACAGC TCTCCACGCA GTGACTACGC AACCTACACC AACAGCAACC 
     3721 ACGCTGCCCC CAGTAGCAAT GCCTCTCCCC CTGAAGGCTT CGCCTCCCAC AGCCTGCAGA 
     3781 CCAGTGATGT GGTCATTCAC CGCAAAGAGA ATGAGGGCTT CGGCTTTGTC ATCATCAGCT 
     3841 CCCTGAACAG GCCTGAGTCT GGATCCACTA TAACTGTGCC CCATAAAATC GGACGCATCA 
     3901 TTGATGGGAG TCCTGCAGAT CGCTGTGCAA AACTAAAAGT GGGAGACCGG ATCCTAGCAG 
     3961 TGAATGGCCA GTCTATCATC AACATGCCTC ACGCTGACAT CGTGAAGCTC ATCAAGGATG 
     4021 CAGGTCTTAG TGTCACCCTT CGCATCATTC CTCAGGAGGA GCTCAACAGC CCCACCTCGG 
     4081 CACCCAGCTC AGAGAAGCAG AGTCCCATGG CGCAGCAGAG TCCCCTGGCA CAGCAGAGTC 
     4141 CCCTGGCCCA GCCAAGCCCA GCCACCCCCA ACAGCCCCAT CGCCCAGCCA GCACCACCTC 
     4201 AACCACTTCA GCTGCAAGGA CACGAAAATA GTTACAGGTC GGAAGTGAAA GCAAGGCAAG 
     4261 ATGTGAAACC AGACATCCGA CAGCCTCCAT TCACAGACTA CAGGCAGCCC CCGCTGGATT 
     4321 ACAGGCAACC CCCAGGAGGG GACTACCAGC AGCCCCCACC CTTGGACTAC AGGCAGCCTC 
     4381 CCCTACTGGA CTACAGGCAG CACTCCCCCG ACACCAGGCA GTACCCTCTG TCGGACTACA 
     4441 GACAACCCCA GGATTTTGAT TATTTCACTG TGGACATGGA GAAAGGAGCC AAAGGATTTG 
     4501 GATTCAGCAT TCGTGGAGGA AGGGAATACA AAATGGATTT GTATGTGTTG AGACTGGCAG 
     4561 AAGATGGACC AGCAATAAGG AATGGGAGGA TGAGGGTAGG AGATCAAATC ATTGAAATCA 
     4621 ATGGGGAAAG CACAAGGGAC ATGACACATG CCAGAGCAAT AGAACTCATC AAATCTGGAG 
     4681 GAAGACGAGT GAGGCTGCTG CTCAAGAGAG GCACGGGACA GGTCCCAGAA TATGACGAAC 
     4741 CCGCCCCCTG GAGTTCTCCC GCTGCCGCCG CCCCAGGTCT GCCGGAAGTA GGCGTCTCCC 
     4801 TGGACGACGG CCTCGCTCCA TTCTCTCCAT CACATCCAGC CCCACCCTCC GACCCTTCCC 
     4861 ACCAGATAAG CCCAGGCCCA ACTTGGGATA TCAAACGGGA ACACGACGTT AGGAAACCAA 
     4921 AGGAGCTTTC AGCCTGCGGC CAGAAGAAGC AGCGCCTCGG GGAGCAGAGG GAGCGCTCGG 
     4981 CGAGTCCGCA GAGGGCCGCG CGGCCGAGGC TCGAGGAGGC GCCCGGCGGC CAGGGGCGGC 
     5041 CCGAGGCCGG CAGGCCCGCC TCGGAGGCCA GGGCGCCCGG GCTCGCGGCG GCAGACGCGG 
     5101 CGGACGCGGC GCGGGCGGGC GGGAAGGAGG CGCCCCGTGC GGCGGCGGGC TCCGAGCTCT 
     5161 GCCGGCGCGA AGGCCCGGGG GCTGCGCCGG CGTTTGCCGG CCCGGGCGGC GGCGGCAGCG 
     5221 GCGCGCTGGA GGCCGAGGGC AGGGCGGGTG CGCGCGCGGG TCCCCGGCCG GGCCCGCGAC 
     5281 CCCCGGGGGG CGCCCCGGCG CGCAAGGCCG CCGTCGCGCC GGGGCCCTGG AAGGTGCCGG 
     5341 GTTCTGACAA GCTGCCCAGC GTCCTCAAAC CCGGCGCCTC GGCCGCCAGC AGAACGCGTA 
     5401 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5461 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5521 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5581 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5641 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5701 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5761 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5821 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5881 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5941 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     6001 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     6061 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     6121 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6181 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6241 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6301 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6361 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6421 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6481 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6541 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6601 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6661 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6721 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6781 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6841 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6901 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6961 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     7021 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     7081 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     7141 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7201 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7261 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7321 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7381 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7441 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7501 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7561 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7621 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7681 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7741 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7801 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7861 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7921 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7981 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     8041 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     8101 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     8161 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8221 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8281 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8341 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8401 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8461 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8521 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8581 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8641 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8701 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8761 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8821 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8881 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8941 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     9001 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     9061 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     9121 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9181 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9241 AATTTT
//