LOCUS       RC222369                7605 bp    DNA     circular  SYN 
DEFINITION  GRIK2 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 2 (GRIK2), transcript variant 1
ACCESSION   NM_021956
VERSION     NM_021956.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3762..3853 
                     /label="Myc-DDK"  
     misc_feature    3883..4468 
                     /label="PolyA signal"  
     misc_feature    3940..3959 
                     /label="Restriction Enzyme"  
     misc_feature    4744..5416 
                     /label="ColE1"  
     misc_feature    5846..6640 
                     /label="Kan/Neo"  
     misc_feature    6675..6977 
                     /label="SV40 ori"  
     misc_feature    7063..7540 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3744..3750 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3744 
                     /label="GRIK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGATTATT TTCCCGATTC TAAGTAATCC AGTCTTCAGG CGCACCGTTA 
     1081 AACTCCTGCT CTGTTTACTG TGGATTGGAT ATTCTCAAGG AACCACACAT GTATTAAGAT 
     1141 TTGGTGGTAT TTTTGAATAT GTGGAATCTG GCCCAATGGG AGCTGAGGAA CTTGCATTCA 
     1201 GATTTGCTGT GAACACAATT AACAGAAACA GAACATTGCT ACCCAATACT ACCCTTACCT 
     1261 ATGATACCCA GAAGATAAAC CTTTATGATA GTTTTGAAGC ATCCAAGAAA GCCTGTGATC 
     1321 AGCTGTCTCT TGGGGTGGCT GCCATCTTCG GGCCTTCACA CAGCTCATCA GCAAACGCAG 
     1381 TGCAGTCCAT CTGCAATGCT CTGGGAGTTC CCCACATACA GACCCGCTGG AAGCACCAGG 
     1441 TGTCAGACAA CAAAGATTCC TTCTATGTCA GTCTCTACCC AGACTTCTCT TCACTCAGCC 
     1501 GTGCCATTTT AGACCTGGTG CAGTTTTTCA AGTGGAAAAC CGTCACGGTT GTGTATGATG 
     1561 ACAGCACTGG TCTCATTCGT TTGCAAGAGC TCATCAAAGC TCCATCAAGG TATAATCTTC 
     1621 GACTCAAAAT TCGTCAGTTA CCTGCTGATA CAAAGGATGC AAAACCCTTA CTAAAAGAAA 
     1681 TGAAAAGAGG CAAGGAGTTT CATGTAATCT TTGATTGTAG CCATGAAATG GCAGCAGGCA 
     1741 TTTTAAAACA GGCATTAGCT ATGGGAATGA TGACAGAATA CTATCATTAT ATCTTTACCA 
     1801 CTCTGGACCT CTTTGCTCTT GATGTTGAGC CCTACCGATA CAGTGGTGTT AACATGACAG 
     1861 GGTTCAGAAT ATTAAATACA GAAAATACCC AAGTCTCCTC CATCATTGAA AAGTGGTCGA 
     1921 TGGAACGATT GCAGGCACCT CCGAAACCCG ATTCAGGTTT GCTGGATGGA TTTATGACGA 
     1981 CTGATGCTGC TCTAATGTAT GATGCTGTGC ATGTGGTGTC TGTGGCCGTT CAACAGTTTC 
     2041 CCCAGATGAC AGTCAGTTCC TTGCAGTGTA ATCGACATAA ACCCTGGCGC TTCGGGACCC 
     2101 GCTTTATGAG TCTAATTAAA GAGGCACATT GGGAAGGCCT CACAGGCAGA ATAACTTTCA 
     2161 ACAAAACCAA TGGCTTGAGA ACAGATTTTG ATTTGGATGT GATCAGTCTG AAGGAAGAAG 
     2221 GTCTAGAAAA GATTGGAACG TGGGATCCAG CCAGTGGCCT GAATATGACA GAAAGTCAAA 
     2281 AGGGAAAGCC AGCGAACATC ACAGATTCCT TATCCAATCG TTCTTTGATT GTTACCACCA 
     2341 TTTTGGAAGA GCCTTATGTC CTTTTTAAGA AGTCTGACAA ACCTCTCTAT GGTAATGATC 
     2401 GATTTGAAGG CTATTGCATT GATCTCCTCA GAGAGTTATC TACAATCCTT GGCTTTACAT 
     2461 ATGAAATTAG ACTTGTGGAA GATGGGAAAT ATGGAGCCCA GGATGATGCC AATGGACAAT 
     2521 GGAATGGAAT GGTTCGTGAA CTAATTGATC ATAAAGCTGA CCTTGCAGTT GCTCCACTGG 
     2581 CTATTACCTA TGTTCGAGAG AAGGTCATCG ACTTTTCCAA GCCCTTTATG ACACTTGGAA 
     2641 TAAGTATTTT GTACCGCAAG CCCAATGGTA CAAACCCAGG CGTCTTCTCC TTCCTGAATC 
     2701 CTCTCTCCCC TGATATCTGG ATGTATGTTC TGCTGGCTTA CTTGGGTGTC AGTTGTGTGC 
     2761 TCTTTGTCAT AGCCAGGTTT AGTCCTTATG AGTGGTATAA TCCACACCCT TGCAACCCTG 
     2821 ACTCAGACGT GGTGGAAAAC AATTTTACCT TGCTAAATAG TTTCTGGTTT GGGGTTGGAG 
     2881 CTCTCATGCG GCAAGGTTCT GAGCTCATGC CCAAAGCACT GTCCACCAGG ATAGTGGGAG 
     2941 GCATTTGGTG GTTTTTCACA CTTATCATCA TTTCTTCGTA TACTGCTAAC TTAGCCGCCT 
     3001 TTCTGACAGT GGAACGCATG GAATCCCCTA TTGACTCTGC TGATGATTTA GCTAAACAAA 
     3061 CCAAGATAGA ATATGGAGCA GTAGAGGATG GTGCAACCAT GACTTTTTTC AAGAAATCAA 
     3121 AAATCTCCAC GTATGACAAA ATGTGGGCCT TTATGAGTAG CAGAAGGCAG TCAGTGCTGG 
     3181 TCAAAAGTAA TGAAGAAGGA ATCCAGCGAG TCCTCACCTC TGATTATGCT TTCCTAATGG 
     3241 AGTCAACAAC CATCGAGTTT GTTACCCAGC GGAACTGTAA CCTGACACAG ATTGGCGGCC 
     3301 TTATAGACTC TAAAGGTTAT GGCGTTGGCA CTCCCATGGG TTCTCCATAT CGAGACAAAA 
     3361 TTACCATAGC AATTCTTCAG CTGCAAGAGG AAGGCAAACT GCATATGATG AAGGAGAAAT 
     3421 GGTGGAGGGG CAATGGTTGC CCAGAAGAGG AAAGCAAAGA GGCCAGTGCC CTGGGGGTTC 
     3481 AGAATATTGG TGGCATCTTC ATTGTTCTGG CAGCCGGCTT GGTGCTTTCA GTTTTTGTGG 
     3541 CAGTGGGAGA ATTTTTATAC AAATCCAAAA AAAACGCTCA ATTGGAAAAG AGGTCCTTCT 
     3601 GTAGTGCCAT GGTAGAAGAA TTGAGGATGT CCCTGAAGTG CCAGCGTCGG TTAAAACATA 
     3661 AGCCACAGGC CCCAGTTATT GTGAAAACAG AAGAAGTTAT CAACATGCAC ACATTTAACG 
     3721 ACAGAAGGTT GCCAGGTAAA GAAACCATGG CAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3781 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3841 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3901 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3961 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4021 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4081 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4141 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4201 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4261 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4321 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4381 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4441 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4501 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4561 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4621 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4681 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4741 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4801 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4861 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4921 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4981 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5041 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5101 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5161 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5221 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5281 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5341 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5401 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5461 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5521 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5581 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5641 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5701 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5761 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5821 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5881 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5941 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6001 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6061 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6121 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6181 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6241 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6301 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6361 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6421 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6481 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6541 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6601 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6661 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6721 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6781 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6841 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6901 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6961 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7021 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7081 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7141 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7201 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7261 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7321 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7381 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7441 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7501 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7561 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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