LOCUS       RC222341                8820 bp    DNA     circular  SYN 
DEFINITION  ATXN2 (Myc-DDK-tagged)-Human ataxin 2 (ATXN2)
ACCESSION   NM_002973
VERSION     NM_002973.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4977..5068 
                     /label="Myc-DDK"  
     misc_feature    5098..5683 
                     /label="PolyA signal"  
     misc_feature    5155..5174 
                     /label="Restriction Enzyme"  
     misc_feature    5959..6631 
                     /label="ColE1"  
     misc_feature    7061..7855 
                     /label="Kan/Neo"  
     misc_feature    7890..8192 
                     /label="SV40 ori"  
     misc_feature    8278..8755 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4959..4965 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4959 
                     /label="ATXN2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCTCAGCG GCCGCAGCTC CTCGGAGTCC CGCGGTGGCC ACCGAGTCTC 
     1081 GCCGCTTCGC CGCAGCCAGG TGGCCCGGGT GGCGCTCGCT CCAGCGGCCG GCGCGGCGGA 
     1141 GCGGGCGGGG CGGCGGTGGC GCGGCCCCGG GACCGTATCC CTCCGCCGCC CCTCCCCCGC 
     1201 CCGGCCCCGG CCCCCCTCCC TCCCGGCAGA GCTCGCCTCC CTCCGCCTCA GACTGTTTTG 
     1261 GTAGCAACGG CAACGGCGGC GGCGCGTTTC GGCCCGGCTC CCGGCGGCTC CTTGGTCTCG 
     1321 GCGGGCCTCC CCGCCCCTTC GTCGTCCTCC TTCTCCCCCT CGCCAGCCCG GGCGCCCCTC 
     1381 CGGCCGCGCC AACCCGCGCC TCCCCGCTCG GCGCCCGCGC GTCCCCGCCG CGTTCCGGCG 
     1441 TCTCCTTGGC GCGCCCGGCT CCCGGCTGTC CCCGCCCGGC GTGCGAGCCG GTGTATGGGC 
     1501 CCCTCACCAT GTCGCTGAAG CCCCAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC 
     1561 AGCAACAGCA GCAGCAGCAG CAGCAGCAGC AGCCGCCGCC CGCGGCTGCC AATGTCCGCA 
     1621 AGCCCGGCGG CAGCGGCCTT CTAGCGTCGC CCGCCGCCGC GCCTTCGCCG TCCTCGTCCT 
     1681 CGGTCTCCTC GTCCTCGGCC ACGGCTCCCT CCTCGGTGGT CGCGGCGACC TCCGGCGGCG 
     1741 GGAGGCCCGG CCTGGGCAGA GGTCGAAACA GTAACAAAGG ACTGCCTCAG TCTACGATTT 
     1801 CTTTTGATGG AATCTATGCA AATATGAGGA TGGTTCATAT ACTTACATCA GTTGTTGGCT 
     1861 CCAAATGTGA AGTACAAGTG AAAAATGGAG GTATATATGA AGGAGTTTTT AAAACTTACA 
     1921 GTCCGAAGTG TGATTTGGTA CTTGATGCCG CACATGAGAA AAGTACAGAA TCCAGTTCGG 
     1981 GGCCGAAACG TGAAGAAATA ATGGAGAGTA TTTTGTTCAA ATGTTCAGAC TTTGTTGTGG 
     2041 TACAGTTTAA AGATATGGAC TCCAGTTATG CAAAAAGAGA TGCTTTTACT GACTCTGCTA 
     2101 TCAGTGCTAA AGTGAATGGC GAACACAAAG AGAAGGACCT GGAGCCCTGG GATGCAGGTG 
     2161 AACTCACAGC CAATGAGGAA CTTGAGGCTT TGGAAAATGA CGTATCTAAT GGATGGGATC 
     2221 CCAATGATAT GTTTCGATAT AATGAAGAAA ATTATGGTGT AGTGTCTACG TATGATAGCA 
     2281 GTTTATCTTC GTATACAGTG CCCTTAGAAA GAGATAACTC AGAAGAATTT TTAAAACGGG 
     2341 AAGCAAGGGC AAACCAGTTA GCAGAAGAAA TTGAGTCAAG TGCCCAGTAC AAAGCTCGAG 
     2401 TGGCCCTGGA AAATGATGAT AGGAGTGAGG AAGAAAAATA CACAGCAGTT CAGAGAAATT 
     2461 CCAGTGAACG TGAGGGGCAC AGCATAAACA CTAGGGAAAA TAAATATATT CCTCCTGGAC 
     2521 AAAGAAATAG AGAAGTCATA TCCTGGGGAA GTGGGAGACA GAATTCACCG CGTATGGGCC 
     2581 AGCCTGGATC GGGCTCCATG CCATCAAGAT CCACTTCTCA CACTTCAGAT TTCAACCCGA 
     2641 ATTCTGGTTC AGACCAAAGA GTAGTTAATG GAGGTGTTCC CTGGCCATCG CCTTGCCCAT 
     2701 CTCCTTCCTC TCGCCCACCT TCTCGCTACC AGTCAGGTCC CAACTCTCTT CCACCTCGGG 
     2761 CAGCCACCCC TACACGGCCG CCCTCCAGGC CCCCCTCGCG GCCATCCAGA CCCCCGTCTC 
     2821 ACCCCTCTGC TCATGGTTCT CCAGCTCCTG TCTCTACTAT GCCTAAACGC ATGTCTTCAG 
     2881 AAGGGCCTCC AAGGATGTCC CCAAAGGCCC AGCGACATCC TCGAAATCAC AGAGTTTCTG 
     2941 CTGGGAGGGG TTCCATATCC AGTGGCCTAG AATTTGTATC CCACAACCCA CCCAGTGAAG 
     3001 CAGCTACTCC TCCAGTAGCA AGGACCAGTC CCTCGGGGGG AACGTGGTCA TCAGTGGTCA 
     3061 GTGGGGTTCC AAGATTATCC CCTAAAACTC ATAGACCCAG GTCTCCCAGA CAGAACAGTA 
     3121 TTGGAAATAC CCCCAGTGGG CCAGTTCTTG CTTCTCCCCA AGCTGGTATT ATTCCAACTG 
     3181 AAGCTGTTGC CATGCCTATT CCAGCTGCAT CTCCTACGCC TGCTAGTCCT GCATCGAACA 
     3241 GAGCTGTTAC CCCTTCTAGT GAGGCTAAAG ATTCCAGGCT TCAAGATCAG AGGCAGAACT 
     3301 CTCCTGCAGG GAATAAAGAA AATATTAAAC CCAATGAAAC ATCACCTAGC TTCTCAAAAG 
     3361 CTGAAAACAA AGGTATATCA CCAGTTGTTT CTGAACATAG AAAACAGATT GATGATTTAA 
     3421 AGAAATTTAA GAATGATTTT AGGTTACAGC CAAGTTCTAC TTCTGAATCT ATGGATCAAC 
     3481 TACTAAACAA AAATAGAGAG GGAGAAAAAT CAAGAGATTT GATCAAAGAC AAAATTGAAC 
     3541 CAAGTGCTAA GGATTCTTTC ATTGAAAATA GCAGCAGCAA CTGTACCAGT GGCAGCAGCA 
     3601 AGCCGAATAG CCCCAGCATT TCCCCTTCAA TACTTAGTAA CACGGAGCAC AAGAGGGGAC 
     3661 CTGAGGTCAC TTCCCAAGGG GTTCAGACTT CCAGCCCAGC ATGTAAACAA GAGAAAGACG 
     3721 ATAAGGAAGA GAAGAAAGAC GCAGCTGAGC AAGTTAGGAA ATCAACATTG AATCCCAATG 
     3781 CAAAGGAGTT CAACCCACGT TCCTTCTCTC AGCCAAAGCC TTCTACTACC CCAACTTCAC 
     3841 CTCGGCCTCA AGCACAACCT AGCCCATCTA TGGTGGGTCA TCAACAGCCA ACTCCAGTTT 
     3901 ATACTCAGCC TGTTTGTTTT GCACCAAATA TGATGTATCC AGTCCCAGTG AGCCCAGGCG 
     3961 TGCAACCTTT ATACCCAATA CCTATGACGC CCATGCCAGT GAATCAAGCC AAGACATATA 
     4021 GAGCAGTACC AAATATGCCC CAACAGCGGC AAGACCAGCA TCATCAGAGT GCCATGATGC 
     4081 ACCCAGCGTC AGCAGCGGGC CCACCGATTG CAGCCACCCC ACCAGCTTAC TCCACGCAAT 
     4141 ATGTTGCCTA CAGTCCTCAG CAGTTCCCAA ATCAGCCCCT TGTTCAGCAT GTGCCACATT 
     4201 ATCAGTCTCA GCATCCTCAT GTCTATAGTC CTGTAATACA GGGTAATGCT AGAATGATGG 
     4261 CACCACCAAC ACACGCCCAG CCTGGTTTAG TATCTTCTTC AGCAACTCAG TACGGGGCTC 
     4321 ATGAGCAGAC GCATGCGATG TATGCATGTC CCAAATTACC ATACAACAAG GAGACAAGCC 
     4381 CTTCTTTCTA CTTTGCCATT TCCACGGGCT CCCTTGCTCA GCAGTATGCG CACCCTAACG 
     4441 CTACCCTGCA CCCACATACT CCACACCCTC AGCCTTCAGC TACCCCCACT GGACAGCAGC 
     4501 AAAGCCAACA TGGTGGAAGT CATCCTGCAC CCAGTCCTGT TCAGCACCAT CAGCACCAGG 
     4561 CCGCCCAGGC TCTCCATCTG GCCAGTCCAC AGCAGCAGTC AGCCATTTAC CACGCGGGGC 
     4621 TTGCGCCAAC TCCACCCTCC ATGACACCTG CCTCCAACAC GCAGTCGCCA CAGAATAGTT 
     4681 TCCCAGCAGC ACAACAGACT GTCTTTACGA TCCATCCTTC TCACGTTCAG CCGGCGTATA 
     4741 CCAACCCACC CCACATGGCC CACGTACCTC AGGCTCATGT ACAGTCAGGA ATGGTTCCTT 
     4801 CTCATCCAAC TGCCCATGCG CCAATGATGC TAATGACGAC ACAGCCACCC GGCGGTCCCC 
     4861 AGGCCGCCCT CGCTCAAAGT GCACTACAGC CCATTCCAGT CTCGACAACA GCGCATTTCC 
     4921 CCTATATGAC GCACCCTTCA GTACAAGCCC ACCACCAACA GCAGTTGACG CGTACGCGGC 
     4981 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5041 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5101 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5161 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5221 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5281 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5341 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5401 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5461 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5521 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5581 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5641 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5701 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5761 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5821 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5881 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5941 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6001 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6061 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6121 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6181 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6241 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6301 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6361 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6421 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6481 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6541 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6601 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6661 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6721 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6781 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6841 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6901 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6961 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7021 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7081 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7141 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7201 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7261 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7321 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7381 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7441 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7501 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7561 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7621 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7681 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7741 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7801 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7861 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7921 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7981 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8041 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8101 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8161 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8221 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8281 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8341 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8401 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8461 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8521 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8581 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8641 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8701 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8761 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//