LOCUS       RC222286                6795 bp    DNA     circular  SYN 
DEFINITION  NOL4 (Myc-DDK-tagged)-Human nucleolar protein 4 (NOL4), transcript variant 1
ACCESSION   NM_003787
VERSION     NM_003787.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2952..3043 
                     /label="Myc-DDK"  
     misc_feature    3073..3658 
                     /label="PolyA signal"  
     misc_feature    3130..3149 
                     /label="Restriction Enzyme"  
     misc_feature    3934..4606 
                     /label="ColE1"  
     misc_feature    5036..5830 
                     /label="Kan/Neo"  
     misc_feature    5865..6167 
                     /label="SV40 ori"  
     misc_feature    6253..6730 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2934..2940 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2934 
                     /label="NOL4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGCGAG CGCGACATGT ACCGCCAGTT CCAGGACTGG TGCCTCAGGA 
     1081 CTTACGGGGA CTCAGGCAAG ACCAAGACGG TGACCCGTAA AAAATACGAA CGGATCGTCC 
     1141 AGCTCCTCAA TGGCTCCGAG TCGAGCTCCA CGGACAACGC CAAATTTAAA TTCTGGGTCA 
     1201 AATCGAAGGG CTTCCAGCTG GGCCAGCCGG ACGAGGTCCG CGGGGGAGGC GGCGGCGCCA 
     1261 AGCAAGTGCT CTACGTGCCT GTCAAGACCA CGGATGGCGT AGGGGTAGAT GAGAAGCTAT 
     1321 CTTTACGACG GGTAGCTGTG GTTGAAGATT TCTTTGACAT TATTTATTCG ATGCATGTGG 
     1381 AAACGGGGCC AAATGGAGAA CAAATTCGGA AACACGCTGG ACAAAAGAGA ACTTACAAAG 
     1441 CAATTTCAGA GAGCTATGCC TTCCTACCAA GAGAAGCGGT GACACGATTT CTAATGAGCT 
     1501 GCTCAGAGTG CCAGAAAAGA ATGCATTTAA ACCCAGATGG AACAGATCAT AAAGATAATG 
     1561 GAAAACCTCC CACTTTGGTG ACCAGCATGA TTGACTACAA CATGCCAATT ACCATGGCCT 
     1621 ACATGAAACA CATGAAGCTG CAGCTGCTAA ACTCACAGCA AGATGAGGAT GAAAGTTCAA 
     1681 TAGAAAGTGA TGAATTTGAC ATGAGTGATT CAACACGGAT GTCAGCTGTG AACTCTGATC 
     1741 TTAGCTCCAA TCTTGAAGAA AGAATGCAAA GTCCCCAGAA TCTTCATGGC CAGCAAGATG 
     1801 ATGATTCTGC TGCAGAGAGC TTTAATGGCA ATGAGACTCT GGGGCACAGT TCAATTGCTT 
     1861 CAGGGGGAAC ACACAGCAGG GAGATGGGAG ACTCCAACAG TGATGGCAAA ACTGGGCTGG 
     1921 AGCAAGATGA ACAGCCACTG AACCTGAGTG ACAGTCCCCT CTCTGCGCAG CTAACTTCGG 
     1981 AATACAGAAT AGATGATCAC AACAGTAATG GGAAAAACAA GTATAAGAAT CTTCTAATTT 
     2041 CTGACCTCAA GATGGAACGA GAGGCGAGAG AAAATGGAAG CAAGTCTCCT GCACATAGTT 
     2101 ACTCCAGCTA TGACTCTGGC AAAAATGAGA GTGTAGACCG AGGAGCTGAG GACCTCTCAC 
     2161 TAAACAGGGG AGATGAGGAC GAAGATGACC ACGAGGACCA TGACGATTCG GAGAAAGTTA 
     2221 ATGAGACAGA CGGCGTTGAA GCCGAGCGGC TGAAAGCTTT TAATATGTTT GTCAGGCTGT 
     2281 TTGTAGATGA AAACTTGGAC CGAATGGTCC CAATCTCTAA GCAGCCCAAA GAAAAGATCC 
     2341 AGGCTATCAT TGACTCATGC AGGCGACAAT TCCCTGAGTA TCAAGAGCGT GCCAGAAAAC 
     2401 GTATACGTAC TTACCTCAAG TCCTGCAGGC GGATGAAAAG AAGTGGTTTT GAGATGTCTC 
     2461 GACCTATTCC TTCCCACCTT ACTTCAGCAG TTGCAGAGAG TATCTTGGCT TCAGCTTGTG 
     2521 AGAGTGAGAG TAGAAATGCC GCCAAGAGGA TGCGTCTGGA GAGACAGCAG GATGAGTCTG 
     2581 CTCCAGCTGA CAAACAGTGT AAACCAGAGG CGACCCAGGC CACTTACTCA ACATCAGCTG 
     2641 TTCCAGGCTC ACAGGACGTG CTGTACATCA ATGGAAATGG GACCTATAGT TACCATAGTT 
     2701 ACAGAGGGCT AGGAGGGGGT CTGCTAAATC TGAATGATGC TTCCAGCAGT GGACCCACTG 
     2761 ATCTCAGCAT GAAGAGACAA TTGGCGACTA GCTCAGGATC CTCCAGCAGC TCAAACTCCA 
     2821 GACCCCAGCT GAGTCCAACT GAAATCAATG CCGTGAGACA GCTTGTTGCA GGATATCGAG 
     2881 AATCAGCTGC ATTTTTATTG CGATCTGCAG ATGAACTGGA AAATCTCATT TTACAACAGA 
     2941 ACACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     3001 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     3061 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     3121 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     3181 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     3241 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     3301 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     3361 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     3421 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     3481 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     3541 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     3601 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     3661 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     3721 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     3781 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     3841 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     3901 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     3961 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     4021 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     4081 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     4141 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     4201 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     4261 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     4321 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     4381 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     4441 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     4501 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     4561 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     4621 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     4681 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     4741 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     4801 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     4861 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     4921 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     4981 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     5041 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     5101 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     5161 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     5221 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     5281 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     5341 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     5401 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     5461 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     5521 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     5581 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     5641 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     5701 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     5761 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     5821 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     5881 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     5941 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     6001 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     6061 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     6121 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6181 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     6241 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     6301 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     6361 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     6421 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     6481 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     6541 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     6601 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     6661 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     6721 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     6781 TTTAACGCGA ATTTT
//