LOCUS       RC222253                8271 bp    DNA     circular  SYN 
DEFINITION  CIITA (Myc-DDK-tagged)-Human class II, major histocompatibility complex, transactivator (CIITA)
ACCESSION   NM_000246
VERSION     NM_000246.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4428..4519 
                     /label="Myc-DDK"  
     misc_feature    4549..5134 
                     /label="PolyA signal"  
     misc_feature    4606..4625 
                     /label="Restriction Enzyme"  
     misc_feature    5410..6082 
                     /label="ColE1"  
     misc_feature    6512..7306 
                     /label="Kan/Neo"  
     misc_feature    7341..7643 
                     /label="SV40 ori"  
     misc_feature    7729..8206 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4410..4416 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4410 
                     /label="CIITA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGTTGCCTG GCTCCACGCC CTGCTGGGTC CTACCTGTCA GAGCCCCAAG 
     1081 GCAGCTCACA GTGTGCCACC ATGGAGTTGG GGCCCCTAGA AGGTGGCTAC CTGGAGCTTC 
     1141 TTAACAGCGA TGCTGACCCC CTGTGCCTCT ACCACTTCTA TGACCAGATG GACCTGGCTG 
     1201 GAGAAGAAGA GATTGAGCTC TACTCAGAAC CCGACACAGA CACCATCAAC TGCGACCAGT 
     1261 TCAGCAGGCT GTTGTGTGAC ATGGAAGGTG ATGAAGAGAC CAGGGAGGCT TATGCCAATA 
     1321 TCGCGGAACT GGACCAGTAT GTCTTCCAGG ACTCCCAGCT GGAGGGCCTG AGCAAGGACA 
     1381 TTTTCAAGCA CATAGGACCA GATGAAGTGA TCGGTGAGAG TATGGAGATG CCAGCAGAAG 
     1441 TTGGGCAGAA AAGTCAGAAA AGACCCTTCC CAGAGGAGCT TCCGGCAGAC CTGAAGCACT 
     1501 GGAAGCCAGC TGAGCCCCCC ACTGTGGTGA CTGGCAGTCT CCTAGTGGGA CCAGTGAGCG 
     1561 ACTGCTCCAC CCTGCCCTGC CTGCCACTGC CTGCGCTGTT CAACCAGGAG CCAGCCTCCG 
     1621 GCCAGATGCG CCTGGAGAAA ACCGACCAGA TTCCCATGCC TTTCTCCAGT TCCTCGTTGA 
     1681 GCTGCCTGAA TCTCCCTGAG GGACCCATCC AGTTTGTCCC CACCATCTCC ACTCTGCCCC 
     1741 ATGGGCTCTG GCAAATCTCT GAGGCTGGAA CAGGGGTCTC CAGTATATTC ATCTACCATG 
     1801 GTGAGGTGCC CCAGGCCAGC CAAGTACCCC CTCCCAGTGG ATTCACTGTC CACGGCCTCC 
     1861 CAACATCTCC AGACCGGCCA GGCTCCACCA GCCCCTTCGC TCCATCAGCC ACTGACCTGC 
     1921 CCAGCATGCC TGAACCTGCC CTGACCTCCC GAGCAAACAT GACAGAGCAC AAGACGTCCC 
     1981 CCACCCAATG CCCGGCAGCT GGAGAGGTCT CCAACAAGCT TCCAAAATGG CCTGAGCCGG 
     2041 TGGAGCAGTT CTACCGCTCA CTGCAGGACA CGTATGGTGC CGAGCCCGCA GGCCCGGATG 
     2101 GCATCCTAGT GGAGGTGGAT CTGGTGCAGG CCAGGCTGGA GAGGAGCAGC AGCAAGAGCC 
     2161 TGGAGCGGGA ACTGGCCACC CCGGACTGGG CAGAACGGCA GCTGGCCCAA GGAGGCCTGG 
     2221 CTGAGGTGCT GTTGGCTGCC AAGGAGCACC GGCGGCCGCG TGAGACACGA GTGATTGCTG 
     2281 TGCTGGGCAA AGCTGGTCAG GGCAAGAGCT ATTGGGCTGG GGCAGTGAGC CGGGCCTGGG 
     2341 CTTGTGGCCG GCTTCCCCAG TACGACTTTG TCTTCTCTGT CCCCTGCCAT TGCTTGAACC 
     2401 GTCCGGGGGA TGCCTATGGC CTGCAGGATC TGCTCTTCTC CCTGGGCCCA CAGCCACTCG 
     2461 TGGCGGCCGA TGAGGTTTTC AGCCACATCT TGAAGAGACC TGACCGCGTT CTGCTCATCC 
     2521 TAGACGGCTT CGAGGAGCTG GAAGCGCAAG ATGGCTTCCT GCACAGCACG TGCGGACCGG 
     2581 CACCGGCGGA GCCCTGCTCC CTCCGGGGGC TGCTGGCCGG CCTTTTCCAG AAGAAGCTGC 
     2641 TCCGAGGTTG CACCCTCCTC CTCACAGCCC GGCCCCGGGG CCGCCTGGTC CAGAGCCTGA 
     2701 GCAAGGCCGA CGCCCTATTT GAGCTGTCCG GCTTCTCCAT GGAGCAGGCC CAGGCATACG 
     2761 TGATGCGCTA CTTTGAGAGC TCAGGGATGA CAGAGCACCA AGACAGAGCC CTGACGCTCC 
     2821 TCCGGGACCG GCCACTTCTT CTCAGTCACA GCCACAGCCC TACTTTGTGC CGGGCAGTGT 
     2881 GCCAGCTCTC AGAGGCCCTG CTGGAGCTTG GGGAGGACGC CAAGCTGCCC TCCACGCTCA 
     2941 CGGGACTCTA TGTCGGCCTG CTGGGCCGTG CAGCCCTCGA CAGCCCCCCC GGGGCCCTGG 
     3001 CAGAGCTGGC CAAGCTGGCC TGGGAGCTGG GCCGCAGACA TCAAAGTACC CTACAGGAGG 
     3061 ACCAGTTCCC ATCCGCAGAC GTGAGGACCT GGGCGATGGC CAAAGGCTTA GTCCAACACC 
     3121 CACCGCGGGC CGCAGAGTCC GAGCTGGCCT TCCCCAGCTT CCTCCTGCAA TGCTTCCTGG 
     3181 GGGCCCTGTG GCTGGCTCTG AGTGGCGAAA TCAAGGACAA GGAGCTCCCG CAGTACCTAG 
     3241 CATTGACCCC AAGGAAGAAG AGGCCCTATG ACAACTGGCT GGAGGGCGTG CCACGCTTTC 
     3301 TGGCTGGGCT GATCTTCCAG CCTCCCGCCC GCTGCCTGGG AGCCCTACTC GGGCCATCGG 
     3361 CGGCTGCCTC GGTGGACAGG AAGCAGAAGG TGCTTGCGAG GTACCTGAAG CGGCTGCAGC 
     3421 CGGGGACACT GCGGGCGCGG CAGCTGCTGG AGCTGCTGCA CTGCGCCCAC GAGGCCGAGG 
     3481 AGGCTGGAAT TTGGCAGCAC GTGGTACAGG AGCTCCCCGG CCGCCTCTCT TTTCTGGGCA 
     3541 CCCGCCTCAC GCCTCCTGAT GCACATGTAC TGGGCAAGGC CTTGGAGGCG GCGGGCCAAG 
     3601 ACTTCTCCCT GGACCTCCGC AGCACTGGCA TTTGCCCCTC TGGATTGGGG AGCCTCGTGG 
     3661 GACTCAGCTG TGTCACCCGT TTCAGGGCTG CCTTGAGCGA CACGGTGGCG CTGTGGGAGT 
     3721 CCCTGCAGCA GCATGGGGAG ACCAAGCTAC TTCAGGCAGC AGAGGAGAAG TTCACCATCG 
     3781 AGCCTTTCAA AGCCAAGTCC CTGAAGGATG TGGAAGACCT GGGAAAGCTT GTGCAGACTC 
     3841 AGAGGACGAG AAGTTCCTCG GAAGACACAG CTGGGGAGCT CCCTGCTGTT CGGGACCTAA 
     3901 AGAAACTGGA GTTTGCGCTG GGCCCTGTCT CAGGCCCCCA GGCTTTCCCC AAACTGGTGC 
     3961 GGATCCTCAC GGCCTTTTCC TCCCTGCAGC ATCTGGACCT GGATGCGCTG AGTGAGAACA 
     4021 AGATCGGGGA CGAGGGTGTC TCGCAGCTCT CAGCCACCTT CCCCCAGCTG AAGTCCTTGG 
     4081 AAACCCTCAA TCTGTCCCAG AACAACATCA CTGACCTGGG TGCCTACAAA CTCGCCGAGG 
     4141 CCCTGCCTTC GCTCGCTGCA TCCCTGCTCA GGCTAAGCTT GTACAATAAC TGCATCTGCG 
     4201 ACGTGGGAGC CGAGAGCTTG GCTCGTGTGC TTCCGGACAT GGTGTCCCTC CGGGTGATGG 
     4261 ACGTCCAGTA CAACAAGTTC ACGGCTGCCG GGGCCCAGCA GCTCGCTGCC AGCCTTCGGA 
     4321 GGTGTCCTCA TGTGGAGACG CTGGCGATGT GGACGCCCAC CATCCCATTC AGTGTCCAGG 
     4381 AACACCTGCA ACAACAGGAT TCACGGATCA GCCTGAGAAC GCGTACGCGG CCGCTCGAGC 
     4441 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4501 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4561 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4621 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4681 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4741 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4801 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4861 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4921 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4981 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5041 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5101 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5161 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5521 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5761 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6061 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6121 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6181 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6241 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6301 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6361 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6421 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6481 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6541 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6601 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6661 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6721 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6781 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6841 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6901 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6961 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7021 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7081 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7141 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7201 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7261 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7321 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7381 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7441 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7501 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7621 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7681 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7741 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7801 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7861 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7921 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7981 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8041 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8101 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8161 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8221 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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