LOCUS       RC222233L2                9624 bp    DNA     circular  SYN 
DEFINITION  AGO3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 1
ACCESSION   NM_024852
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7913..8093 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8821..9480 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    8141..8760 
                     /label="ColE1"  
     misc_feature    6788..6808 
                     /label="LR50"  
     misc_feature    5928..6653 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6797..7388 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5900..5907 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5900 
                     /label="AGO3"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAAATCGGC TCCGCAGGAC CCGCTGGGGC 
     3361 CCAGCCCCTA CTCATGGTGC CCAGAAGACC TGGCTATGGC ACCATGGGCA AACCCATTAA 
     3421 ACTGCTGGCT AACTGTTTTC AAGTTGAAAT CCCAAAGATT GATGTCTACC TCTATGAGGT 
     3481 AGATATTAAA CCAGACAAGT GTCCTAGGAG AGTGAACAGG GAGGTGGTTG ACTCAATGGT 
     3541 TCAGCATTTT AAAGTAACTA TATTTGGAGA CCGTAGACCA GTTTATGATG GAAAAAGAAG 
     3601 TCTTTACACC GCCAATCCAC TTCCTGTGGC AACTACAGGG GTAGATTTAG ACGTTACTTT 
     3661 ACCTGGGGAA GGTGGAAAAG ATCGACCTTT CAAGGTGTCA ATCAAATTTG TCTCTCGGGT 
     3721 GAGTTGGCAC CTACTGCATG AAGTACTGAC AGGACGGACC TTGCCTGAGC CACTGGAATT 
     3781 AGACAAGCCA ATCAGCACTA ACCCTGTCCA TGCCGTTGAT GTGGTGCTAC GACATCTGCC 
     3841 CTCCATGAAA TACACACCTG TGGGGCGTTC ATTTTTCTCC GCTCCAGAAG GATATGACCA 
     3901 CCCTCTGGGA GGGGGCAGGG AAGTGTGGTT TGGATTCCAT CAGTCTGTTC GGCCTGCCAT 
     3961 GTGGAAAATG ATGCTTAATA TCGATGTTTC TGCCACTGCC TTCTACAAAG CACAACCTGT 
     4021 AATTCAGTTC ATGTGTGAAG TTCTTGATAT TCATAATATT GATGAGCAAC CAAGACCTCT 
     4081 GACTGATTCT CATCGGGTAA AATTCACCAA AGAGATAAAA GGTTTGAAGG TTGAAGTGAC 
     4141 TCATTGTGGA ACAATGAGAC GGAAATACCG TGTTTGTAAT GTAACAAGGA GGCCTGCCAG 
     4201 TCATCAAACC TTTCCTTTAC AGTTAGAAAA CGGCCAAACT GTGGAGAGAA CAGTAGCGCA 
     4261 GTATTTCAGA GAAAAGTATA CTCTTCAGCT GAAGTACCCG CACCTTCCCT GTCTGCAAGT 
     4321 CGGGCAGGAA CAGAAACACA CCTACCTGCC ACTAGAAGTC TGTAATATTG TGGCAGGGCA 
     4381 ACGATGTATC AAGAAGCTAA CAGACAATCA GACTTCCACT ATGATCAAGG CAACAGCAAG 
     4441 ATCTGCACCA GATAGACAAG AGGAAATTAG CAGATTGGTA AGAAGTGCAA ATTATGAAAC 
     4501 AGATCCATTT GTTCAGGAGT TTCAATTTAA AGTTCGGGAT GAAATGGCTC ATGTAACTGG 
     4561 ACGCGTACTT CCAGCACCTA TGCTCCAGTA TGGAGGACGG AATCGGACAG TAGCAACACC 
     4621 GAGCCATGGA GTATGGGACA TGCGAGGGAA ACAATTCCAC ACAGGAGTTG AAATCAAAAT 
     4681 GTGGGCTATC GCTTGTTTTG CCACACAGAG GCAGTGCAGA GAAGAAATAT TGAAGGGTTT 
     4741 CACAGACCAG CTGCGTAAGA TTTCTAAGGA TGCAGGGATG CCCATCCAGG GCCAGCCATG 
     4801 CTTCTGCAAA TATGCACAGG GGGCAGACAG CGTAGAGCCC ATGTTCCGGC ATCTCAAGAA 
     4861 CACATATTCT GGCCTACAGC TTATTATCGT CATCCTGCCG GGGAAGACAC CAGTGTATGC 
     4921 GGAAGTGAAA CGTGTAGGAG ACACACTTTT GGGTATGGCT ACACAATGTG TTCAAGTCAA 
     4981 GAATGTAATA AAAACATCTC CTCAAACTCT GTCAAACTTG TGCCTAAAGA TAAATGTTAA 
     5041 ACTCGGAGGG ATCAATAATA TTCTTGTACC TCATCAAAGA CCTTCTGTGT TCCAGCAACC 
     5101 AGTGATCTTT TTGGGAGCCG ATGTCACTCA TCCACCTGCT GGTGATGGAA AGAAGCCTTC 
     5161 TATTGCTGCT GTTGTAGGTA GTATGGATGC ACACCCAAGC AGATACTGTG CCACAGTAAG 
     5221 AGTTCAGAGA CCCCGACAGG AGATCATCCA GGACTTGGCC TCCATGGTCC GGGAACTTCT 
     5281 TATTCAATTT TATAAGTCAA CTCGGTTCAA GCCTACTCGT ATCATCTTTT ATCGGGATGG 
     5341 TGTTTCAGAG GGGCAGTTTA GGCAGGTATT ATATTATGAA CTACTAGCAA TTCGAGAAGC 
     5401 CTGCATCAGT TTGGAGAAAG ACTATCAACC TGGAATAACC TACATTGTAG TTCAGAAGAG 
     5461 ACATCACACT CGATTATTTT GTGCTGATAG GACAGAAAGG GTTGGAAGAA GTGGCAATAT 
     5521 CCCAGCTGGA ACAACAGTTG ATACAGACAT TACACACCCA TATGAGTTCG ATTTTTACCT 
     5581 CTGTAGCCAT GCTGGAATAC AGGGTACCAG TCGTCCTTCA CACTATCATG TTTTATGGGA 
     5641 TGATAACTGC TTTACTGCAG ATGAACTTCA GCTGCTAACT TACCAGCTCT GCCACACTTA 
     5701 CGTACGCTGT ACACGATCTG TTTCTATACC TGCACCAGCG TATTATGCTC ACCTGGTAGC 
     5761 ATTTAGAGCC AGATATCATC TTGTGGACAA AGAACATGAC AGTGCTGAAG GAAGTCACGT 
     5821 TTCAGGACAA AGCAATGGGC GAGATCCACA AGCTCTTGCC AAGGCTGTAC AGATTCACCA 
     5881 AGATACCTTA CGCACAATGT ACTTCGCTAG CGGACCGACG CGTACGCGGC CGCTCGAGAT 
     5941 GAGCGGGGGC GAGGAGCTGT TCGCCGGCAT CGTGCCCGTG CTGATCGAGC TGGACGGCGA 
     6001 CGTGCACGGC CACAAGTTCA GCGTGCGCGG CGAGGGCGAG GGCGACGCCG ACTACGGCAA 
     6061 GCTGGAGATC AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTGGT 
     6121 GACCACCCTC TGCTACGGCA TCCAGTGCTT CGCCCGCTAC CCCGAGCACA TGAAGATGAA 
     6181 CGACTTCTTC AAGAGCGCCA TGCCCGAGGG CTACATCCAG GAGCGCACCA TCCAGTTCCA 
     6241 GGACGACGGC AAGTACAAGA CCCGCGGCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA 
     6301 CCGCATCGAG CTGAAGGGCA AGGACTTCAA GGAGGACGGC AACATCCTGG GCCACAAGCT 
     6361 GGAGTACAGC TTCAACAGCC ACAACGTGTA CATCCGCCCC GACAAGGCCA ACAACGGCCT 
     6421 GGAGGCTAAC TTCAAGACCC GCCACAACAT CGAGGGCGGC GGCGTGCAGC TGGCCGACCA 
     6481 CTACCAGACC AACGTGCCCC TGGGCGACGG CCCCGTGCTG ATCCCCATCA ACCACTACCT 
     6541 GAGCACTCAG ACCAAGATCA GCAAGGACCG CAACGAGGCC CGCGACCACA TGGTGCTCCT 
     6601 GGAGTCCTTC AGCGCCTGCT GCCACACCCA CGGCATGGAC GAGCTGTACA GGTCCGGACT 
     6661 CAGATAAGTT TAAACGGCCG GCCGCGGTCT GTACAAGTAG GATTCGTCGA GGGACCTAAT 
     6721 AACTTCGTAT AGCATACATT ATACGAAGTT ATACATGTTT AAGGGTTCCG GTTCCACTAG 
     6781 GTACAATTCG ATATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA 
     6841 TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG 
     6901 CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC 
     6961 TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC 
     7021 ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT 
     7081 TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT 
     7141 GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG 
     7201 AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG 
     7261 TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG 
     7321 CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT 
     7381 TGGGCCGCCT CCCCGCATCG ATACCGTCGA CCTCGATCGA GACCTAGAAA AACATGGAGC 
     7441 AATCACAAGT AGCAATACAG CAGCTACCAA TGCTGATTGT GCCTGGCTAG AAGCACAAGA 
     7501 GGAGGAGGAG GTGGGTTTTC CAGTCACACC TCAGGTACCT TTAAGACCAA TGACTTACAA 
     7561 GGCAGCTGTA GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA 
     7621 CTCCCAACGA AGACAAGATA TCCTTGATCT GTGGATCTAC CACACACAAG GCTACTTCCC 
     7681 TGATTGGCAG AACTACACAC CAGGGCCAGG GATCAGATAT CCACTGACCT TTGGATGGTG 
     7741 CTACAAGCTA GTACCAGTTG AGCAAGAGAA GGTAGAAGAA GCCAATGAAG GAGAGAACAC 
     7801 CCGCTTGTTA CACCCTGTGA GCCTGCATGG GATGGATGAC CCGGAGAGAG AAGTATTAGA 
     7861 GTGGAGGTTT GACAGCCGCC TAGCATTTCA TCACATGGCC CGAGAGCTGC ATCCGGACTG 
     7921 TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA 
     7981 CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT 
     8041 GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC 
     8101 TAGCAGCATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     8161 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     8221 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     8281 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     8341 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     8401 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     8461 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     8521 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     8581 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     8641 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     8701 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     8761 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     8821 TTTGGTCATG ATTACGCCCC GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC 
     8881 ATTCTGCCGA CATGGAAGCC ATCACAAACG GCATGATGAA CCTGAATCGC CAGCGGCATC 
     8941 AGCACCTTGT CGCCTTGCGT ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG 
     9001 TCCATATTGG CCACGTTTAA ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG 
     9061 AAAAACATAT TCTCAATAAA CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC 
     9121 ACATCTTGCG AATATATGTG TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC 
     9181 GATGAAAACG TTTCAGTTTG CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT 
     9241 ATCACCAGCT CACCGTCTTT CATTGCCATA CGGAACTCCG GATGAGCATT CATCAGGCGG 
     9301 GCAAGAATGT GAATAAAGGC CGGATAAAAC TTGTGCTTAT TTTTCTTTAC GGTCTTTAAA 
     9361 AAGGCCGTAA TATCCAGCTG AACGGTCTGG TTATAGGTAC ATTGAGCAAC TGACTGAAAT 
     9421 GCCTCAAAAT GTTCTTTACG ATGCCATTGG GATATATCAA CGGTGGTATA TCCAGTGATT 
     9481 TTTTTCTCCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     9541 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     9601 TTTCCCCGAA AAGTGCCACC TGAC
//