LOCUS       RC222209                6594 bp    DNA     circular  SYN 
DEFINITION  CES3 (Myc-DDK-tagged)-Human carboxylesterase 3 (CES3), transcript variant 1
ACCESSION   NM_024922
VERSION     NM_024922.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2751..2842 
                     /label="Myc-DDK"  
     misc_feature    2872..3457 
                     /label="PolyA signal"  
     misc_feature    2929..2948 
                     /label="Restriction Enzyme"  
     misc_feature    3733..4405 
                     /label="ColE1"  
     misc_feature    4835..5629 
                     /label="Kan/Neo"  
     misc_feature    5664..5966 
                     /label="SV40 ori"  
     misc_feature    6052..6529 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2733..2739 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2733 
                     /label="CES3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAGAGCA GTGAGAGTGG AGTCCGGGGT CCTGGTCGGG GTGGTCTGTC 
     1081 TGCTCCTGGC ATGCCCTGCC ACAGCCACTG GGCCCGAAGT TGCTCAGCCT GAAGTAGACA 
     1141 CCACCCTGGG TCGTGTGCGA GGCCGGCAGG TGGGCGTGAA GGGCACAGAC CGCCTTGTGA 
     1201 ATGTCTTTCT GGGCATTCCA TTTGCCCAGC CGCCACTGGG CCCTGACCGG TTCTCAGCCC 
     1261 CACACCCAGC ACAGCCCTGG GAGGGTGTGC GGGATGCCAG CACTGCGCCC CCAATGTGCC 
     1321 TACAAGACGT GGAGAGCATG AACAGCAGCA GATTTGTCCT CAACGGAAAA CAGCAGATCT 
     1381 TCTCCGTTTC AGAGGACTGC CTGGTCCTCA ACGTCTATAG CCCAGCTGAG GTCCCCGCAG 
     1441 GGTCCGGTAG GCCGGTCATG GTATGGGTCC ATGGAGGCGC TCTGATAACT GGCGCTGCCA 
     1501 CCTCCTACGA TGGATCAGCT CTGGCTGCCT ATGGGGATGT GGTCGTGGTT ACAGTCCAGT 
     1561 ACCGCCTTGG GGTCCTTGGC TTCTTCAGCA CTGGAGATGA GCATGCACCT GGCAACCAGG 
     1621 GCTTCCTAGA TGTGGTAGCT GCTTTGCGCT GGGTGCAAGA AAACATCGCC CCCTTCGGGG 
     1681 GTGACCTCAA CTGTGTCACT GTCTTTGGTG GATCTGCCGG TGGGAGCATC ATCTCTGGCC 
     1741 TGGTCCTGTC CCCAGTGGCT GCAGGGCTGT TCCACAGAGC CATCACACAG AGTGGGGTCA 
     1801 TCACCACCCC AGGGATCATC GACTCTCACC CTTGGCCCCT AGCTCAGAAA ATCGCAAACA 
     1861 CCTTGGCCTG CAGCTCCAGC TCCCCGGCTG AGATGGTGCA GTGCCTTCAG CAGAAAGAAG 
     1921 GAGAAGAGCT GGTCCTTAGC AAGAAGCTGA AAAATACTAT CTATCCTCTC ACCGTTGATG 
     1981 GCACTGTCTT CCCCAAAAGC CCCAAGGAAC TCCTGAAGGA GAAGCCCTTC CACTCTGTGC 
     2041 CCTTCCTCAT GGGTGTCAAC AACCATGAGT TCAGCTGGCT CATCCCCAGG GGCTGGGGTC 
     2101 TCCTGGATAC AATGGAGCAG ATGAGCCGGG AGGACATGCT GGCCATCTCA ACACCCGTCT 
     2161 TGACCAGTCT GGATGTGCCC CCTGAGATGA TGCCCACCGT CATAGATGAA TACCTAGGAA 
     2221 GCAACTCGGA CGCACAAGCC AAATGCCAGG CGTTCCAGGA ATTCATGGGT GACGTATTCA 
     2281 TCAATGTTCC CACCGTCAGT TTTTCAAGAT ACCTTCGAGA TTCTGGAAGC CCTGTCTTTT 
     2341 TCTATGAGTT CCAGCATCGA CCCAGTTCTT TTGCGAAGAT CAAACCTGCC TGGGTGAAGG 
     2401 CTGATCATGG GGCCGAGGGT GCTTTTGTGT TCGGAGGTCC CTTCCTCATG GACGAGAGCT 
     2461 CCCGCCTGGC CTTTCCAGAG GCCACAGAGG AGGAGAAGCA GCTAAGCCTC ACCATGATGG 
     2521 CCCAGTGGAC CCACTTTGCC CGGACAGGGG ACCCCAATAG CAAGGCTCTG CCTCCTTGGC 
     2581 CCCAATTCAA CCAGGCGGAA CAATATCTGG AGATCAACCC AGTGCCACGG GCCGGACAGA 
     2641 AGTTCAGGGA GGCCTGGATG CAGTTCTGGT CAGAGACGCT CCCCAGCAAG ATACAACAGT 
     2701 GGCACCAGAA GCAGAAGAAC AGGAAGGCCC AGGAGGACCT CACGCGTACG CGGCCGCTCG 
     2761 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     2821 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     2881 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     2941 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3001 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3061 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3121 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     3181 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     3241 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     3301 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     3361 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     3421 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     3481 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     3541 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     3601 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     3661 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     3721 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     3781 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     3841 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     3901 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     3961 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4021 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4081 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4141 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     4201 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     4261 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     4321 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     4381 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     4441 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     4501 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     4561 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     4621 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     4681 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     4741 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     4801 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     4861 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     4921 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     4981 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5041 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5101 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     5161 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     5221 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     5281 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     5341 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     5401 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     5461 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     5521 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     5581 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     5641 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     5701 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     5761 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     5821 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     5881 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     5941 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6001 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6061 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6121 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     6181 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     6241 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     6301 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     6361 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     6421 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     6481 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     6541 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//