LOCUS       RC222193                9363 bp    DNA     circular  SYN 
DEFINITION  MBD5 (Myc-DDK-tagged)-Human methyl-CpG binding domain protein 5 (MBD5)
ACCESSION   NM_018328
VERSION     NM_018328.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5520..5611 
                     /label="Myc-DDK"  
     misc_feature    5641..6226 
                     /label="PolyA signal"  
     misc_feature    5698..5717 
                     /label="Restriction Enzyme"  
     misc_feature    6502..7174 
                     /label="ColE1"  
     misc_feature    7604..8398 
                     /label="Kan/Neo"  
     misc_feature    8433..8735 
                     /label="SV40 ori"  
     misc_feature    8821..9298 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5502..5508 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5502 
                     /label="MBD5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATGGAGGC AAAGAGTGTG ACGGAGGGGA CAAGGAAGGA GGTCTTCCAG 
     1081 CTATACAAGT TCCTGTGGGT TGGCAGCGTC GTGTGGATCA AAATGGAGTG CTTTATGTCA 
     1141 GTCCCAGTGG GTCTTTGTTA TCTTGCTTGG AGCAGGTTAA AACATACCTG CTTACTGATG 
     1201 GAACATGCAA GTGTGGCTTG GAATGTCCTC TTATTCTTCC CAAGGTATTT AATTTTGATC 
     1261 CTGGAGCTGC TGTGAAACAG AGAACCGCAG AAGATGTTAA GGCAGATGAA GATGTCACAA 
     1321 AGCTATGCAT ACATAAAAGA AAAATTATTG CAGTGGCCAC ACTTCATAAA AGCATGGAAG 
     1381 CCCCACATCC TTCTCTGGTG CTCACCAGTC CCGGAGGAGG AACAAATGCA ACTCCAGTAG 
     1441 TACCTTCTCG GGCAGCAACT CCAAGATCAG TAAGAAATAA GTCTCATGAA GGAATTACAA 
     1501 ATTCTGTAAT GCCTGAATGT AAGAATCCTT TCAAGTTAAT GATTGGATCA TCAAATGCCA 
     1561 TGGGAAGGCT ATATGTACAA GAACTGCCTG GAAGCCAACA ACAAGAACTC CACCCTGTCT 
     1621 ACCCCCGACA GAGATTGGGC AGCAGTGAAC ATGGACAGAA ATCTCCATTC CGTGGCAGCC 
     1681 ATGGAGGCCT GCCCAGCCCA GCGTCATCAG GTTCCCAGAT ATATGGAGAT GGTTCAATCT 
     1741 CTCCAAGGAC TGACCCACTT GGAAGTCCTG ATGTTTTCAC AAGAAGTAAT CCTGGTTTTC 
     1801 ATGGAGCTCC CAATTCTAGT CCTATTCACC TGAATAGGAC TCCTCTTTCT CCACCTTCAG 
     1861 TAATGCTACA TGGTTCTCCT GTACAGTCAT CCTGTGCAAT GGCTGGAAGG ACTAATATAC 
     1921 CTCTTTCCCC AACCTTGACT ACAAAGAGTC CAGTAATGAA AAAACCAATG TGTAATTTTT 
     1981 CAACTAATAT GGAAATACCA CGAGCAATGT TCCACCACAA ACCACCCCAA GGCCCACCTC 
     2041 CCCCTCCTCC ACCTTCTTGT GCTCTTCAGA AAAAGCCATT AACATCTGAG AAAGATCCAC 
     2101 TTGGCATTCT TGACCCTATT CCTAGTAAAC CAGTGAATCA GAACCCTGTT ATCATTAATC 
     2161 CAACCAGTTT CCATTCAAAT GTCCACTCTC AGGTACCTAT GATGAATGTA AGCATGCCTC 
     2221 CTGCTGTTGT TCCTTTGCCA AGTAATCTCC CATTGCCAAC TGTAAAACCT GGTCACATGA 
     2281 ATCATGGGAG TCATGTACAA AGAGTTCAGC ATTCAGCTTC AACCTCCCTG TCCCCTTCTC 
     2341 CAGTGACATC CCCCGTGCAC ATGATGGGGA CTGGAATTGG AAGGATTGAG GCATCGCCCC 
     2401 AAAGATCACG CTCATCTTCC ACATCATCAG ATCATGGAAA TTTCATGATG CCACCTGTAG 
     2461 GACCCCAGGC CACTTCTAGT GGTATTAAGG TTCCACCCAG GTCACCAAGG TCAACAATAG 
     2521 GGTCCCCAAG GCCATCAATG CCATCAAGCC CTTCTACCAA GTCCGATGGA CATCATCAGT 
     2581 ACAAGGATAT CCCTAACCCA TTAATTGCTG GAATAAGTAA TGTACTAAAT ACCCCAAGCA 
     2641 GTGCAGCTTT TCCTACTGCA TCTGCCGGAA GTAGTTCTGT AAAGAGTCAG CCTGGTTTGC 
     2701 TGGGAATGCC TTTAAATCAG ATCTTGAACC AGCACAATGC TGCCTCCTTT CCAGCAAGTA 
     2761 GTTTACTCTC AGCAGCAGCC AAAGCACAGC TAGCAAATCA AAACAAACTT GCTGGTAACA 
     2821 ACAGTAGCAG CAGTAGCAAT TCTGGAGCTG TTGCCGGCAG TGGCAACACT GAAGGACATA 
     2881 GCACTTTAAA CACCATGTTC CCTCCTACTG CCAACATGCT TCTCCCAACA GGTGAAGGGC 
     2941 AAAGTGGTCG AGCAGCACTA AGAGATAAGC TGATGTCTCA GCAAAAAGAC GCATTGCGGA 
     3001 AAAGAAAACA ACCACCTACG ACAGTGTTGA GTTTGCTCAG ACAGTCTCAA ATGGATAGTT 
     3061 CTGCAGTTCC TAAACCTGGA CCTGACTTGC TAAGGAAGCA GGGTCAGGGT TCATTTCCCA 
     3121 TCAGTTCAAT GTCTCAGTTA CTACAGTCTA TGAGTTGTCA AAGCTCTCAC TTGAGTAGCA 
     3181 ATAGTACCCC GGGTTGTGGG GCCTCAAATA CTGCTTTGCC TTGCTCTGCT AACCAGCTGC 
     3241 ATTTTACAGA TCCCAGTATG AACTCTAGTG TTCTTCAGAA CATACCTTTA AGAGGGGAAG 
     3301 CCGTGCACTG CCACAATGCA AACACTAACT TTGTTCACAG TAACAGTCCA GTCCCCAACC 
     3361 ACCATCTTGC AGGTTTAATA AATCAGATTC AGGCTAGCGG GAACTGTGGG ATGCTCAGTC 
     3421 AGTCGGGCAT GGCTTTAGGA AATTCCTTAC ATCCCAATCC ACCTCAGTCA AGAATTTCAA 
     3481 CGTCCTCCAC TCCAGTGATA CCAAACAGCA TTGTTAGCAG CTATAATCAA ACAAGTTCTG 
     3541 AAGCAGGCGG TTCAGGACCA TCATCCTCCA TAGCCATAGC GGGCACCAAC CACCCTGCCA 
     3601 TCACAAAGAC AACATCTGTT CTTCAAGATG GCGTCATAGT CACCACTGCA GCTGGAAACC 
     3661 CACTGCAGAG TCAGCTACCC ATTGGGAGTG ATTTTCCTTT TGTTGGCCAG GAGCACGCAC 
     3721 TTCATTTTCC ATCCAACAGC ACTTCAAACA ACCATCTTCC ACACCCCTTG AACCCCAGCC 
     3781 TCCTCAGTTC TCTACCTATC TCTTTGCCAG TGAATCAACA GCATCTCCTA AACCAGAATC 
     3841 TATTAAATAT CCTCCAGCCT TCAGCAGGAG AAGGTGATAT GTCATCAATA AACAATACTT 
     3901 TGAGTAACCA TCAACTGACT CATCTACAGT CGCTGTTAAA CAACAATCAG ATGTTTCCTC 
     3961 CAAATCAGCA ACAGCAGCAA CTTCTCCAGG GGTACCAGAA TCTCCAGGCG TTCCAAGGAC 
     4021 AGTCCACAAT TCCTTGCCCA GCTAACAATA ACCCCATGGC TTGTCTGTTT CAGAACTTTC 
     4081 AGGTGAGAAT GCAGGAAGAT GCAGCTCTCC TAAACAAAAG AATAAGCACT CAGCCTGGGC 
     4141 TCACAGCACT TCCTGAGAAT CCAAACACTA CACTTCCACC TTTTCAAGAT ACACCTTGTG 
     4201 AGTTGCAACC GAGGATTGAC CCATCTCTTG GTCAACAGGT GAAGGATGGC CTCGTTGTGG 
     4261 GTGGCCCAGG TGATGCTTCC GTAGATGCCA TTTACAAAGC AGTTGTCGAT GCAGCCAGCA 
     4321 AAGGAATGCA GGTTGTCATC ACCACTGCAG TCAACAGTAC AACTCAGATC AGCCCCATTC 
     4381 CAGCTCTGAG TGCCATGAGT GCCTTCACTG CCTCAATTGG TGACCCATTA AATCTCTCCA 
     4441 GTGCTGTCAG TGCGGTCATT CATGGACGGA ACATGGGAGG TGTTGATCAT GATGGTAGGC 
     4501 TGAGGAATTC AAGAGGGGCT CGGCTGCCCA AGAATCTAGA CCATGGGAAA AATGTGAACG 
     4561 AAGGAGATGG GTTTGAATAT TTCAAGTCAG CAAGTTGCCA CACATCCAAA AAACAGTGGG 
     4621 ACGGGGAGCA AAGCCCCAGA GGGGAGCGAA ACAGGTGGAA GTACGAGGAA TTTTTAGATC 
     4681 ATCCAGGCCA TATCCACAGT AGTCCTTGTC ATGAAAGGCC CAACAATGTC TCTACACTGC 
     4741 CATTTCTGCC TGGGGAACAG CACCCAATAC TGTTACCACC AAGAAACTGT CCAGGGGATA 
     4801 AAATTCTAGA GGAAAATTTC AGGTATAATA ACTACAAAAG AACTATGATG AGTTTTAAGG 
     4861 AGAGACTAGA GAACACTGTG GAAAGATGTG CACACATAAA TGGGAATAGA CCTCGACAGA 
     4921 GTCGGGGATT TGGAGAGCTG CTAAGCACTG CAAAGCAAGA CCTGGTCCTA GAGGAGCAGT 
     4981 CTCCAAGTTC CTCAAATAGT TTGGAAAATT CTCTGGTCAA AGACTACATC CATTACAATG 
     5041 GAGACTTTAA TGCCAAAAGC GTTAATGGGT GTGTGCCTAG CCCTTCAGAT GCTAAAAGCA 
     5101 TTAGTAGTGA AGATGACCTA AGGAACCCAG ACTCCCCCTC TTCAAATGAA TTGATACATT 
     5161 ATAGACCAAG GACGTTCAAT GTTGGCGACT TGGTCTGGGG CCAAATCAAA GGACTGACTT 
     5221 CCTGGCCTGG AAAATTAGTA AGAGAAGACG ACGTTCACAA TTCATGTCAA CAAAGCCCCG 
     5281 AGGAAGGGAA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG GAAGCCTACA 
     5341 GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA GAAGCTGCTA 
     5401 TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA ATCCCACAGG 
     5461 GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA ACGCGTACGC 
     5521 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5581 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5641 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5701 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5761 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5821 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5881 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5941 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     6001 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     6061 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     6121 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6181 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6241 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6301 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6361 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6421 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6481 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6541 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6601 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6661 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6721 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6781 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6841 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6901 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6961 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     7021 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     7081 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     7141 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7201 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7261 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7321 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7381 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7441 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7501 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7561 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7621 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7681 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7741 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7801 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7861 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7921 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7981 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     8041 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     8101 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     8161 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8221 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8281 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8341 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8401 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8461 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8521 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8581 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8641 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8701 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8761 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8821 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8881 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8941 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     9001 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     9061 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     9121 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9181 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9241 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9301 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9361 TTT
//