LOCUS       RC222135                8802 bp    DNA     circular  SYN 
DEFINITION  OTOF (Myc-DDK-tagged)-Human otoferlin (OTOF), transcript variant 3
ACCESSION   NM_194322
VERSION     NM_194322.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4959..5050 
                     /label="Myc-DDK"  
     misc_feature    5080..5665 
                     /label="PolyA signal"  
     misc_feature    5137..5156 
                     /label="Restriction Enzyme"  
     misc_feature    5941..6613 
                     /label="ColE1"  
     misc_feature    7043..7837 
                     /label="Kan/Neo"  
     misc_feature    7872..8174 
                     /label="SV40 ori"  
     misc_feature    8260..8737 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4941..4947 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4941 
                     /label="OTOF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGACCGAT ACTCAGGATG GCCCTAGCGA GAGCTCCCAG ATCATGAGGT 
     1081 CCCTCACTCC CCTGATCAAC AGGGAGGAGG CATTTGGGGA GGCTGGGGAG GCGGGGCTGT 
     1141 GGCCCAGCAT CACCCACACT CCTGATTCAC AGGAAGAAGG CCTGAACGAC ATACAGGAGA 
     1201 TGATCAAAAC GGAGAAGTCC TACCCTGAGC GTCGCCTGCG GGGCGTCCTG GAGGAGCTGA 
     1261 GCTGTGGCTG CTGCCGCTTC CTCTCCCTCG CTGACAAGGA CCAGGGCCAC TCATCCCGCA 
     1321 CCAGGCTTGA CCGGGAGCGC CTCAAGTCCT GCATGAGGGA GCTGGAAAAC ATGGGGCAGC 
     1381 AGGCCAGGAT GCTGCGGGCC CAGGTGAAGC GGCACACGGT GCGGGACAAG CTGAGGCTGT 
     1441 GCCAGAACTT CCTGCAGAAG CTGCGCTTCC TGGCGGACGA GCCCCAGCAC AGCATTCCCG 
     1501 ACATCTTCAT CTGGATGATG AGCAACAACA AGCGTGTCGC CTATGCCCGT GTGCCCTCCA 
     1561 AGGACCTGCT CTTCTCCATC GTGGAGGAGG AGACTGGCAA GGACTGCGCC AAGGTCAAGA 
     1621 CGCTCTTCCT TAAGCTGCCA GGGAAGCGGG GCTTCGGCTC GGCAGGCTGG ACAGTGCAGG 
     1681 CCAAGGTGGA GCTGTACCTG TGGCTGGGCC TCAGCAAACA GCGCAAGGAG TTCCTGTGCG 
     1741 GCCTGCCCTG TGGCTTCCAG GAGGTCAAGG CAGCCCAGGG CCTGGGCCTG CATGCCTTCC 
     1801 CACCCGTCAG CCTGGTCTAC ACCAAGAAGC AGGCGTTCCA GCTCCGAGCG CACATGTACC 
     1861 AGGCCCGCAG CCTCTTTGCC GCCGACAGCA GCGGACTCTC AGACCCCTTT GCCCGCGTCT 
     1921 TCTTCATCAA TCAGAGTCAG TGCACAGAGG TGCTGAATGA GACCCTGTGT CCCACCTGGG 
     1981 ACCAGATGCT GGTGTTCGAC AACCTGGAGC TCTATGGTGA AGCTCATGAG CTGAGGGACG 
     2041 ATCCGCCCAT CATTGTCATT GAAATCTATG ACCAGGATTC CATGGGCAAA GCTGACTTCA 
     2101 TGGGCCGGAC CTTCGCCAAA CCCCTGGTGA AGATGGCAGA CGAGGCGTAC TGCCCACCCC 
     2161 GCTTCCCACC TCAGCTCGAG TACTACCAGA TCTACCGTGG CAACGCCACA GCTGGAGACC 
     2221 TGCTGGCGGC CTTCGAGCTG CTGCAGATTG GACCAGCAGG GAAGGCTGAC CTGCCCCCCA 
     2281 TCAATGGCCC GGTGGACGTG GACCGAGGTC CCATCATGCC CGTGCCCATG GGCATCCGGC 
     2341 CCGTGCTCAG CAAGTACCGA GTGGAGGTGC TGTTCTGGGG CCTACGGGAC CTAAAGCGGG 
     2401 TGAACCTGGC CCAGGTGGAC CGGCCACGGG TGGACATCGA GTGTGCAGGG AAGGGGGTGC 
     2461 AGTCGTCCCT GATCCACAAT TATAAGAAGA ACCCCAACTT CAACACCCTC GTCAAGTGGT 
     2521 TTGAAGTGGA CCTCCCAGAG AACGAGCTGC TGCACCCGCC CTTGAACATC CGTGTGGTGG 
     2581 ACTGCCGGGC CTTCGGTCGC TACACACTGG TGGGCTCCCA TGCCGTCAGC TCCCTGCGAC 
     2641 GCTTCATCTA CCGGCCCCCA GACCGCTCGG CCCCCAGCTG GAACACCACG GTCAGGCTTC 
     2701 TCCGGCGCTG CCGTGTGCTG TGCAATGGGG GCTCCTCCTC TCACTCCACA GGGGAGGTTG 
     2761 TGGTGACTAT GGAGCCAGAG GTACCCATCA AGAAACTGGA GACCATGGTG AAGCTGGACG 
     2821 CGACTTCTGA AGCTGTTGTC AAGGTGGATG TGGCTGAGGA GGAGAAGGAG AAGAAGAAGA 
     2881 AGAAGAAGGG CACTGCGGAG GAGCCAGAGG AGGAGGAGCC AGACGAGAGC ATGCTGGACT 
     2941 GGTGGTCCAA GTACTTTGCC TCCATTGACA CCATGAAGGA GCAACTTCGA CAACAAGAGC 
     3001 CCTCTGGAAT TGACTTGGAG GAGAAGGAGG AAGTGGACAA TACCGAGGGC CTGAAGGGGT 
     3061 CAATGAAGGG CAAGGAGAAG GCAAGGGCTG CCAAAGAGGA GAAGAAGAAG AAAACTCAGA 
     3121 GCTCTGGCTC TGGCCAGGGG TCCGAGGCCC CCGAGAAGAA GAAACCCAAG ATTGATGAGC 
     3181 TTAAGGTATA CCCCAAAGAG CTGGAGTCCG AGTTTGATAA CTTTGAGGAC TGGCTGCACA 
     3241 CTTTCAACTT GCTTCGGGGC AAGACCGGGG ATGATGAGGA TGGCTCCACC GAGGAGGAGC 
     3301 GCATTGTGGG ACGCTTCAAG GGCTCCCTCT GCGTGTACAA AGTGCCACTC CCAGAGGACG 
     3361 TGTCCCGGGA AGCCGGCTAC GACTCCACCT ACGGCATGTT CCAGGGCATC CCGAGCAATG 
     3421 ACCCCATCAA TGTGCTGGTC CGAGTCTATG TGGTCCGGGC CACGGACCTG CACCCTGCTG 
     3481 ACATCAACGG CAAAGCTGAC CCCTACATCG CCATCCGGCT AGGCAAGACT GACATCCGCG 
     3541 ACAAGGAGAA CTACATCTCC AAGCAGCTCA ACCCTGTCTT TGGGAAGTCC TTTGACATCG 
     3601 AGGCCTCCTT CCCCATGGAA TCCATGCTGA CGGTGGCTGT GTATGACTGG GACCTGGTGG 
     3661 GCACTGATGA CCTCATTGGG GAAACCAAGA TCGACCTGGA GAACCGCTTC TACAGCAAGC 
     3721 ACCGCGCCAC CTGCGGCATC GCCCAGACCT ACTCCACACA TGGCTACAAT ATCTGGCGGG 
     3781 ACCCCATGAA GCCCAGCCAG ATCCTGACCC GCCTCTGCAA AGACGGCAAA GTGGACGGCC 
     3841 CCCACTTTGG GCCCCCTGGG AGAGTGAAGG TGGCCAACCG CGTCTTCACT GGGCCCTCTG 
     3901 AGATTGAGGA CGAGAACGGT CAGAGGAAGC CCACAGACGA GCATGTGGCG CTGTTGGCCC 
     3961 TGAGGCACTG GGAGGACATC CCCCGCGCAG GCTGCCGCCT GGTGCCAGAG CATGTGGAGA 
     4021 CGAGGCCGCT GCTCAACCCC GACAAGCCGG GCATCGAGCA GGGCCGCCTG GAGCTGTGGG 
     4081 TGGACATGTT CCCCATGGAC ATGCCAGCCC CTGGGACGCC TCTGGACATC TCACCTCGGA 
     4141 AGCCCAAGAA GTACGAGCTG CGGGTCATCA TCTGGAACAC AGATGAGGTG GTCTTGGAGG 
     4201 ACGACGACTT CTTCACAGGG GAGAAGTCCA GTGACATCTT CGTGAGGGGG TGGCTGAAGG 
     4261 GCCAGCAGGA GGACAAGCAG GACACAGACG TCCACTACCA CTCCCTCACT GGCGAGGGCA 
     4321 ACTTCAACTG GCGCTACCTG TTCCCCTTCG ACTACCTGGC GGCGGAGGAG AAGATCGTCA 
     4381 TCTCCAAGAA GGAGTCCATG TTCTCCTGGG ACGAGACCGA GTACAAGATC CCCGCGCGGC 
     4441 TCACCCTGCA GATCTGGGAT GCGGACCACT TCTCCGCTGA CGACTTCCTG GGGGCCATCG 
     4501 AGCTGGACCT GAACCGGTTC CCGCGGGGCG CAAAGACAGC CAAGCAGTGC ACCATGGAGA 
     4561 TGGCCACCGG GGAGGTGGAC GTGCCCCTCG TGTCCATCTT CAAGCAAAAG CGCGTCAAAG 
     4621 GCTGGTGGCC CCTCCTGGCC CGCAATGAGA ACGATGAGTT TGAGCTCACG GGCAAGGTGG 
     4681 AGGCTGAGCT GCATTTACTG ACAGCAGAGG AGGCAGAGAA GAACCCAGTG GGCCTGGCCC 
     4741 GCAATGAACC TGACCCCCTA GAGAAACCCA ACCGGCCCGA CACGAGCTTC ATCTGGTTCC 
     4801 TGAACCCTCT CAAGTCGGCT CGCTACTTCT TGTGGCACAC GTATCGCTGG CTGCTCCTCA 
     4861 AACTGTTGCT GCTCCTGCTG CTGCTCCTCC TCCTCGCCCT GTTCCTCTAC TCTGTGCCTG 
     4921 GCTACCTGGT CAAGAAAATC CTCGGGGCCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4981 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     5041 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     5101 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     5161 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     5221 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     5281 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     5341 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     5401 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     5461 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5521 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5581 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5641 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5701 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5761 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5821 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5881 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5941 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     6001 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     6061 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     6121 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     6181 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     6241 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     6301 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     6361 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     6421 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6481 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6541 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6601 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6661 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6721 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6781 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6841 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6901 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6961 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     7021 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     7081 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     7141 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     7201 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     7261 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     7321 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     7381 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     7441 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7501 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7561 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7621 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7681 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7741 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7801 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7861 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7921 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7981 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     8041 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8101 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8161 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     8221 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     8281 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     8341 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     8401 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     8461 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8521 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8581 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8641 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8701 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8761 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//