LOCUS       RC222133                7758 bp    DNA     circular  SYN 
DEFINITION  XYLT1 (Myc-DDK-tagged)-Human xylosyltransferase I (XYLT1)
ACCESSION   NM_022166
VERSION     NM_022166.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3915..4006 
                     /label="Myc-DDK"  
     misc_feature    4036..4621 
                     /label="PolyA signal"  
     misc_feature    4093..4112 
                     /label="Restriction Enzyme"  
     misc_feature    4897..5569 
                     /label="ColE1"  
     misc_feature    5999..6793 
                     /label="Kan/Neo"  
     misc_feature    6828..7130 
                     /label="SV40 ori"  
     misc_feature    7216..7693 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3897..3903 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3897 
                     /label="XYLT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGCGGCG CCGTGCGCCC GGAGGCTGGC CCGGCGCTCG CACTCGGCGC 
     1081 TGCTCGCGGC GCTCACGGTG CTGCTGCTGC AGACGCTGGT CGTGTGGAAT TTCAGCAGCC 
     1141 TCGACTCCGG GGCCGGGGAG CGCCGCGGGG GCGCAGCGGT CGGCGGCGGG GAGCAGCCGC 
     1201 CCCCGGCCCC GGCCCCGCGC CGGGAGCGCC GGGACCTGCC CGCCGAGCCG GCTGCAGCCC 
     1261 GAGGAGGAGG AGGAGGCGGC GGCGGAGGAG GAGGAGGACG GGGGCCCCAG GCGCGGGCGC 
     1321 GGGGAGGCGG CCCCGGAGAA CCGCGGGGAC AGCAGCCGGC CAGCCGGGGG GCACTGCCCG 
     1381 CCCGGGCTCT GGATCCACAC CCAAGTCCGC TCATCACCCT GGAGACTCAG GATGGCTACT 
     1441 TTTCTCATCG GCCGAAAGAG AAAGTGCGAA CAGACAGCAA CAACGAGAAC TCTGTCCCCA 
     1501 AAGACTTTGA GAATGTGGAC AACAGCAACT TCGCACCCAG GACTCAAAAG CAGAAGCACC 
     1561 AGCCTGAGTT GGCGAAGAAG CCACCGAGTA GACAGAAGGA GCTTTTGAAA AGGAAGCTGG 
     1621 AACAGCAGGA GAAAGGAAAA GGACATACAT TCCCTGGGAA AGGCCCCGGT GAGGTGCTGC 
     1681 CTCCCGGGGA CAGAGCCGCA GCCAACAGCA GCCACGGGAA GGATGTGTCC AGACCGCCTC 
     1741 ATGCCAGGAA AACTGGGGGC AGCTCCCCCG AGACCAAGTA TGACCAGCCC CCTAAGTGTG 
     1801 ACATCTCAGG CAAGGAGGCC ATCTCTGCCC TGTCCCGTGC TAAGTCCAAG CACTGCCGCC 
     1861 AGGAGATTGG GGAGACTTAC TGCCGCCACA AGTTAGGGCT GCTGATGCCT GAGAAGGTGA 
     1921 CTCGGTTCTG CCCCCTCGAG GGTAAAGCCA ACAAGAACGT GCAGTGGGAC GAGGACTCCG 
     1981 TGGAGTACAT GCCAGCCAAC CCGGTCAGAA TCGCCTTTGT CCTGGTGGTC CACGGCCGTG 
     2041 CCTCTCGGCA GTTGCAGCGC ATGTTCAAGG CCATCTACCA CAAAGACCAC TTCTACTACA 
     2101 TCCACGTGGA CAAGCGCTCT AATTACCTGC ATCGGCAAGT GCTCCAGGTC TCCAGGCAGT 
     2161 ACAGCAATGT CCGCGTCACC CCCTGGAGAA TGGCCACCAT CTGGGGAGGA GCCAGCCTCC 
     2221 TGTCCACCTA CCTGCAGAGC ATGCGGGACC TCCTGGAGAT GACCGACTGG CCCTGGGACT 
     2281 TCTTCATCAA CCTGAGTGCG GCCGACTACC CCATCAGGAC AAATGACCAG TTGGTGGCGT 
     2341 TTCTCTCCCG ATACCGAGAT ATGAATTTCT TGAAGTCACA CGGCCGGGAC AATGCAAGGT 
     2401 TCATTCGGAA GCAGGGCCTG GATCGGCTCT TCCTGGAGTG CGACGCTCAC ATGTGGCGCC 
     2461 TGGGAGATCG GCGGATCCCA GAGGGCATTG CCGTGGATGG CGGTTCGGAC TGGTTCCTGC 
     2521 TGAACCGGAG GTTTGTAGAA TATGTGACCT TCTCCACAGA CGATCTGGTG ACCAAGATGA 
     2581 AACAGTTCTA CTCCTACACC CTGCTTCCTG CTGAGTCCTT CTTCCATACG GTCCTGGAGA 
     2641 ACAGCCCCCA CTGCGACACC ATGGTGGACA ACAACCTGCG CATCACCAAC TGGAATCGCA 
     2701 AGCTGGGCTG CAAGTGCCAG TACAAGCACA TCGTGGACTG GTGCGGCTGC TCCCCCAATG 
     2761 ACTTCAAGCC GCAGGACTTC CACCGCTTCC AGCAGACAGC CCGGCCTACC TTCTTTGCCC 
     2821 GCAAGTTTGA AGCCGTGGTG AATCAGGAAA TCATTGGGCA GCTGGACTAT TACCTGTACG 
     2881 GGAACTACCC TGCAGGTACC CCGGGCCTGC GCTCCTACTG GGAGAATGTC TACGATGAGC 
     2941 CTGACGGCAT CCACAGCCTG AGCGACGTGA CACTCACCTT GTACCACTCC TTTGCCCGCC 
     3001 TGGGTCTTCG ACGGGCCGAG ACGTCCCTGC ACACGGATGG GGAGAACAGC TGCCGATACT 
     3061 ACCCAATGGG CCACCCAGCA TCTGTGCACC TCTACTTCCT TGCTGACCGC TTCCAGGGCT 
     3121 TTCTGATCAA GCATCATGCT ACCAATCTGG CTGTGAGCAA ACTAGAGACT CTGGAGACCT 
     3181 GGGTGATGCC GAAAAAAGTC TTCAAGATCG CAAGCCCACC CAGTGACTTT GGGAGGCTTC 
     3241 AGTTTTCCGA GGTCGGCACT GACTGGGATG CCAAGGAGAG GCTATTCCGC AACTTTGGGG 
     3301 GTCTTCTGGG GCCCATGGAT GAGCCGGTGG GTATGCAGAA GTGGGGGAAG GGACCTAATG 
     3361 TGACCGTGAC CGTCATTTGG GTGGATCCCG TCAATGTCAT CGCAGCCACC TACGACATCC 
     3421 TCATTGAGTC CACTGCCGAA TTCACACACT ACAAGCCCCC TTTGAACTTG CCCCTGAGGC 
     3481 CTGGGGTCTG GACAGTGAAA ATTCTCCACC ACTGGGTGCC AGTTGCAGAG ACCAAATTCC 
     3541 TCGTTGCGCC TCTGACCTTC TCGAACAGGC AGCCCATCAA ACCTGAGGAG GCACTGAAGC 
     3601 TGCACAATGG GCCCCTCCGC AATGCCTACA TGGAGCAGAG CTTCCAGAGC CTAAACCCCG 
     3661 TCCTCAGCCT GCCCATCAAC CCCGCCCAGG TGGAACAGGC ACGGAGGAAC GCAGCCTCCA 
     3721 CGGGCACAGC GCTGGAGGGA TGGCTGGACT CGTTGGTGGG CGGGATGTGG ACTGCCATGG 
     3781 ACATCTGTGC CACGGGCCCC ACAGCCTGCC CGGTCATGCA GACCTGCAGC CAGACGGCCT 
     3841 GGAGCTCCTT CAGCCCTGAC CCCAAGTCGG AGCTGGGGGC AGTCAAACCT GATGGCCGGC 
     3901 TCAGGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3961 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4021 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4081 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4141 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4201 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4261 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4321 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4381 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4441 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4501 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4561 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4621 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4681 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4741 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4801 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4861 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4921 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4981 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5041 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5101 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5161 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5221 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5281 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5341 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5401 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5461 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5521 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5581 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5641 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5701 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5761 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5821 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5881 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5941 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6001 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6061 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6121 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6181 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6241 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6301 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6361 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6421 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6481 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6541 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6601 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6661 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6721 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6781 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6841 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6901 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6961 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7021 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7081 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7141 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7201 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7261 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7321 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7381 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7441 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7501 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7561 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7621 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7681 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7741 AAATTTAACG CGAATTTT
//