LOCUS       RC222027                7224 bp    DNA     circular  SYN 
DEFINITION  SP3 (Myc-DDK-tagged)-Human Sp3 transcription factor (SP3), transcript variant 1
ACCESSION   NM_003111
VERSION     NM_003111.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3381..3472 
                     /label="Myc-DDK"  
     misc_feature    3502..4087 
                     /label="PolyA signal"  
     misc_feature    3559..3578 
                     /label="Restriction Enzyme"  
     misc_feature    4363..5035 
                     /label="ColE1"  
     misc_feature    5465..6259 
                     /label="Kan/Neo"  
     misc_feature    6294..6596 
                     /label="SV40 ori"  
     misc_feature    6682..7159 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3363..3369 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3363 
                     /label="SP3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCGCTCCC GAAAAGCCCG TGAAACAAGA GGAAATGGCT GCCTTGGACG 
     1081 TGGATAGCGG CGGCGGCGGT GGCGGCGGCG GCGGCCACGG CGAGTATCTG CAGCAGCAGC 
     1141 AACAGCACGG AAACGGTGCG GTGGCGGCGG CAGCGGCGGC CCAGGACACT CAGCCGTCAC 
     1201 CGCTCGCTCT GCTGGCCGCT ACCTGCAGCA AGATAGGGCC GCCATCGCCG GGCGACGACG 
     1261 AGGAGGAGGC GGCCGCCGCA GCCGGGGCCC CCGCCGCCGC CGGAGCGACA GGTGATTTGG 
     1321 CTTCTGCACA GTTAGGAGGA GCACCAAACC GATGGGAGGT TTTGTCAGCC ACACCTACAA 
     1381 CTATAAAAGA TGAAGCTGGT AATCTAGTCC AGATTCCAAG TGCTGCTACT TCAAGTGGGC 
     1441 AGTATGTTCT TCCCCTTCAG AATTTGCAGA ATCAACAAAT ATTTTCCGTT GCACCAGGAT 
     1501 CAGATTCATC AAATGGTGCA GTGTCCAGTG TTCAATATCA AGTGATACCA CAGATCCAGT 
     1561 CAGCAGATGG TCAGCAGGTT CAAATTGGTT TCACAGGCTC TTCAGATAAT GGGGGTATAA 
     1621 ATCAAGAAAG CAGTCAAATT CAGATCATTC CTGGCTCTAA TCAAACCTTA CTTGCCTCTG 
     1681 GAACACCTTC TGCTAACATC CAGAATCTCA TACCACAGAC TGGTCAAGTC CAGGTTCAGG 
     1741 GAGTTGCAAT TGGTGGTTCA TCTTTTCCTG GTCAAACCCA AGTAGTTGCT AATGTGCCTC 
     1801 TTGGTCTGCC AGGAAATATT ACGTTTGTAC CAATCAATAG TGTCGATCTA GATTCTTTGG 
     1861 GACTCTCGGG CAGTTCTCAG ACAATGACTG CAGGCATTAA TGCCGACGGA CATTTGATAA 
     1921 ACACAGGACA AGCTATGGAT AGTTCAGACA ATTCAGAAAG GACTGGTGAG CGGGTTTCTC 
     1981 CTGATATTAA TGAAACTAAT ACTGATACAG ATTTATTTGT GCCAACATCC TCTTCATCAC 
     2041 AGTTGCCTGT TACGATAGAT AGTACAGGTA TATTACAACA AAACACAAAT AGCTTGACTA 
     2101 CATCTAGTGG GCAGGTTCAT TCTTCAGATC TTCAGGGAAA TTATATCCAG TCGCCTGTTT 
     2161 CTGAAGAGAC ACAGGCACAG AATATTCAGG TTTCTACAGC ACAGCCTGTT GTACAGCATC 
     2221 TACAACTTCA AGAGTCTCAG CAGCCAACCA GTCAAGCCCA AATTGTGCAA GGTATTACAC 
     2281 CACAGACAAT CCATGGTGTG CAAGCCAGTG GTCAAAATAT ATCACAACAG GCTTTGCAAA 
     2341 ATCTTCAGTT GCAGCTGAAT CCTGGAACCT TTTTAATTCA GGCACAGACA GTGACCCCTT 
     2401 CTGGACAGGT AACTTGGCAA ACGTTTCAAG TACAAGGGGT CCAGAACTTG CAGAATTTGC 
     2461 AAATACAGAA TACTGCTGCC CAACAAATAA CTTTGACGCC TGTTCAAACC CTCACACTTG 
     2521 GTCAAGTTGC GGCAGGTGGA GCCTTCACTT CAACTCCAGT TAGTCTAAGC ACTGGTCAGT 
     2581 TGCCAAATCT ACAAACAGTT ACAGTGAACT CTATAGATTC TGCTGGTATA CAGCTACATC 
     2641 CAGGAGAGAA TGCTGACAGT CCTGCAGATA TTAGGATCAA GGAAGAAGAA CCTGATCCTG 
     2701 AAGAGTGGCA GCTCAGTGGT GATTCTACCT TGAATACCAA TGACCTAACA CACTTAAGAG 
     2761 TACAGGTGGT AGATGAAGAA GGGGACCAAC AACATCAAGA AGGAAAAAGA CTTCGGAGGG 
     2821 TAGCTTGCAC CTGTCCCAAC TGTAAAGAAG GTGGTGGAAG AGGTACCAAT CTTGGGAAAA 
     2881 AGAAGCAACA CATTTGTCAT ATACCAGGAT GTGGTAAAGT CTATGGGAAG ACCTCACATC 
     2941 TGAGAGCTCA TCTGCGTTGG CATTCTGGAG AACGCCCTTT TGTTTGTAAC TGGATGTACT 
     3001 GTGGTAAAAG ATTTACTCGA AGTGATGAAT TACAGAGGCA CAGAAGAACA CATACAGGTG 
     3061 AGAAGAAATT TGTTTGTCCA GAATGTTCAA AACGCTTTAT GAGAAGTGAC CACCTTGCCA 
     3121 AACATATTAA AACACACCAG AATAAAAAAG GTATTCACTC TAGCAGTACA GTGCTGGCAT 
     3181 CTGTGGAAGC TGCGCGAGAT GATACTTTGA TTACTGCAGG AGGAACAACG CTTATCCTTG 
     3241 CAAATATTCA ACAAGGTTCT GTTTCAGGGA TAGGAACTGT TAATACTTCC GCCACCAGCA 
     3301 ATCAAGATAT CCTTACCAAC ACTGAAATAC CTTTACAGCT TGTCACAGTT TCTGGAAATG 
     3361 AGACAATGGA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3421 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3481 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3541 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3601 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3661 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3721 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3781 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3841 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3901 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3961 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4021 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4081 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4141 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4201 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4261 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4321 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4381 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4441 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4501 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4561 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4621 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4681 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4741 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4801 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4861 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4921 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4981 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5041 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5101 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5161 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5221 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5281 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5341 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5401 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5461 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5521 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5581 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5641 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5701 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5761 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5821 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5881 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5941 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6001 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6061 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6121 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6181 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6241 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6301 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6361 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6421 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6481 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6541 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6601 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6661 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6721 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6781 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6841 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6901 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6961 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7021 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7081 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7141 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7201 TAACAAAAAT TTAACGCGAA TTTT
//