LOCUS       RC221936                7389 bp    DNA     circular  SYN 
DEFINITION  EPC1 (Myc-DDK-tagged)-Human enhancer of polycomb homolog 1 (Drosophila) (EPC1)
ACCESSION   NM_025209
VERSION     NM_025209.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3546..3637 
                     /label="Myc-DDK"  
     misc_feature    3667..4252 
                     /label="PolyA signal"  
     misc_feature    3724..3743 
                     /label="Restriction Enzyme"  
     misc_feature    4528..5200 
                     /label="ColE1"  
     misc_feature    5630..6424 
                     /label="Kan/Neo"  
     misc_feature    6459..6761 
                     /label="SV40 ori"  
     misc_feature    6847..7324 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3528..3534 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3528 
                     /label="EPC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTAAACTG TCGTTTCGGG CGCGGGCGCT AGACGCCTCG AAGCCGCTGC 
     1081 CGGTTTTCCG CTGTGAGGAT CTGCCCGACC TGCACGAATA CGCCTCGATA AACAGGGCCG 
     1141 TGCCGCAGAT GCCCACCGGA ATGGAGAAGG AAGAGGAGTC GGAACATCAT CTTCAGCGGG 
     1201 CTATTTCAGC ACAGCAGGTG TATGGCGAGA AGAGGGATAA TATGGTTATA CCGGTCCCAG 
     1261 AGGCAGAAAG TAATATTGCT TACTATGAGT CTATATATCC TGGGGAATTT AAGATGCCAA 
     1321 AGCAGCTCAT TCACATACAG CCTTTTAGTT TGGATGCTGA ACAGCCTGAT TATGATTTGG 
     1381 ATTCTGAAGA TGAAGTATTT GTGAATAAAC TGAAAAAGAA AATGGACATC TGCCCATTGC 
     1441 AATTTGAGGA GATGATTGAC CGCCTAGAAA AAGGCAGTGG TCAGCAGCCA GTCAGTCTGC 
     1501 AGGAAGCCAA ACTACTGCTA AAAGAAGATG ATGAACTAAT TAGAGAAGTT TATGAATATT 
     1561 GGATTAAAAA GAGAAAAAAC TGTCGAGGGC CATCTCTTAT TCCATCAGTA AAACAAGAGA 
     1621 AGCGAGATGG TTCCAGCACA AATGATCCTT ATGTGGCTTT TAGAAGGCGT ACTGAAAAAA 
     1681 TGCAGACTCG AAAAAATCGC AAAAATGATG AAGCCTCTTA CGAAAAAATG CTTAAGCTGC 
     1741 GACGAGATCT AAGTCGAGCT GTTACTATTC TAGAGATGAT AAAAAGAAGA GAAAAAAGTA 
     1801 AAAGAGAGCT ATTGCACTTA ACACTGGAAA TTATGGAAAA GAGGTATAAT TTGGGCGACT 
     1861 ACAATGGAGA GATCATGTCT GAGGTTATGG CACAGAGACA GCCAATGAAA CCTACTTATG 
     1921 CCATCCCCAT CATCCCTATT ACTAATAGCA GTCAATTTAA ACACCAGGAA GCAATGGATG 
     1981 TGAAGGAGTT CAAAGTTAAT AAGCAAGATA AAGCCGATCT TATCCGACCG AAACGGAAAT 
     2041 ATGAAAAGAA GCCCAAAGTC TTACCATCGT CTGCCGCTGC TACTCCCCAA CAGACGAGTC 
     2101 CTGCTGCACT GCCAGTCTTC AATGCTAAAG ATCTGAATCA GTATGACTTT CCCAGCTCAG 
     2161 ACGAAGAACC TCTCTCCCAG GTTTTGTCTG GCTCTTCGGA AGCTGAGGAA GACAATGATC 
     2221 CTGATGGTCC TTTTGCTTTC CGTAGGAAAG CAGGCTGTCA GTACTATGCT CCTCACTTAG 
     2281 ACCAAACTGG CAACTGGCCT TGGACTAGTC CTAAAGATGG AGGATTAGGG GATGTGCGAT 
     2341 ATAGATACTG CTTAACTACT CTCACCGTAC CCCAAAGGTG TATTGGATTT GCACGAAGAC 
     2401 GGGTTGGGCG CGGTGGAAGG GTCTTACTGG ACAGAGCTCA TTCAGACTAT GACAGTGTGT 
     2461 TTCACCATCT GGATTTGGAA ATGCTTTCCT CACCACAACA TTCTCCAGTC AATCAGTTTG 
     2521 CCAATACCTC AGAAACAAAT ACCTCGGACA AATCTTTCTC TAAAGACCTC AGTCAGATAC 
     2581 TAGTCAATAT CAAATCATGT AGATGGCGGC ATTTTAGGCC TCGGACACCA TCCCTACATG 
     2641 ACAGTGACAA TGATGAACTC TCCTGTAGAA AATTATATAG GAGTATAAAC CGAACAGGAA 
     2701 CAGCACAACC TGGGACCCAG ACATGCAGTA CCTCTACGCA AAGTAAAAGT AGCAGTGGTT 
     2761 CAGCACACTT TGCATTTACA GCCGAACAAT ACCAGCAACA TCAACAGCAA CTGGCACTCA 
     2821 TGCAGAAACA GCAGCTTGCA CAAATTCAGC AACAGCAAGC AAATAGTAAT TCCTCCACCA 
     2881 ACACATCACA GAACCTTGCA TCTAACCAGC AGAAAAGTGG CTTTCGCCTG AATATACAGG 
     2941 GTTTAGAAAG AACACTACAG GGTTTTGTTT CTAAGACTTT GGATTCTGCT AGTGCACAGT 
     3001 TTGCTGCTTC TGCTTTGGTG ACATCAGAAC AACTGATGGG ATTCAAGATG AAGGATGATG 
     3061 TGGTGCTTGG AATCGGGGTG AATGGCGTCC TTCCAGCCTC AGGAGTATAC AAGGGCTTAC 
     3121 ACCTCAGTAG TACTACACCA ACAGCACTTG TACATACAAG TCCATCAACG GCAGGTTCAG 
     3181 CTTTGTTACA GCCTTCAAAT ATTACACAGA CTTCAAGTTC CCACAGTGCA CTGAGTCATC 
     3241 AAGTAACTGC TGCCAATTCT GCAACAACTC AGGTTCTGAT TGGGAACAAC ATTCGATTAA 
     3301 CTGTACCTTC ATCAGTTGCC ACTGTAAACT CTATTGCCCC AATAAATGCA CGACATATAC 
     3361 CTAGGACTTT AAGTGCTGTT CCATCATCTG CCTTAAAGCT GGCCGCTGCA GCAAACTGTC 
     3421 AAGTTTCCAA GGTTCCATCT TCATCCTCTG TAGATTCAGT TCCAAGGGAA AATCATGAAT 
     3481 CAGAAAAGCC AGCACTGAAC AACATAGCAG ACAACACAGT AGCGATGGAG GTGACGACGC 
     3541 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3601 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3661 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3721 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3781 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3841 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3901 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3961 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4021 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4081 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4141 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4201 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4261 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4321 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4381 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4441 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4501 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4561 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4621 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4681 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4741 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4801 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4861 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4921 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4981 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5041 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5101 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5161 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5221 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5281 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5341 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5401 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5461 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5521 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5581 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5641 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5701 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5761 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5821 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5881 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5941 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6001 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6061 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6121 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6181 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6241 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6301 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6361 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6421 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6481 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6541 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6601 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6661 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6721 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6781 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6841 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6901 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6961 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7021 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7081 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7141 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7201 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7261 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7321 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7381 GCGAATTTT
//