LOCUS       RC221895                8415 bp    DNA     circular  SYN 
DEFINITION  PC (Myc-DDK-tagged)-Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 1
ACCESSION   NM_000920
VERSION     NM_000920.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4572..4663 
                     /label="Myc-DDK"  
     misc_feature    4693..5278 
                     /label="PolyA signal"  
     misc_feature    4750..4769 
                     /label="Restriction Enzyme"  
     misc_feature    5554..6226 
                     /label="ColE1"  
     misc_feature    6656..7450 
                     /label="Kan/Neo"  
     misc_feature    7485..7787 
                     /label="SV40 ori"  
     misc_feature    7873..8350 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4554..4560 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4554 
                     /label="PC"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGAAGTTC CGAACAGTCC ATGGGGGCCT GAGGCTCCTG GGAATCCGCC 
     1081 GAACCTCCAC CGCCCCCGCT GCCTCCCCAA ATGTCCGGCG CCTGGAGTAT AAGCCCATCA 
     1141 AGAAAGTCAT GGTGGCCAAC AGAGGTGAGA TTGCCATCCG TGTGTTCCGG GCCTGCACGG 
     1201 AGCTGGGCAT CCGCACCGTA GCCATCTACT CTGAGCAGGA CACGGGCCAG ATGCACCGGC 
     1261 AGAAAGCAGA TGAAGCCTAT CTCATCGGCC GCGGCCTGGC CCCCGTGCAG GCCTACCTGC 
     1321 ACATCCCAGA CATCATCAAG GTGGCCAAGG AGAACAACGT AGATGCAGTG CACCCTGGCT 
     1381 ACGGGTTCCT CTCTGAGCGA GCGGACTTCG CCCAGGCCTG CCAGGATGCA GGGGTCCGGT 
     1441 TTATTGGGCC AAGCCCAGAA GTGGTCCGCA AGATGGGAGA CAAGGTGGAG GCCCGGGCCA 
     1501 TCGCCATTGC TGCGGGTGTT CCCGTTGTCC CTGGCACAGA TGCCCCCATC ACGTCCCTGC 
     1561 ATGAGGCCCA CGAGTTCTCC AACACCTACG GCTTCCCCAT CATCTTCAAG GCGGCCTATG 
     1621 GGGGTGGAGG GCGTGGCATG AGGGTGGTGC ACAGCTACGA GGAGCTGGAG GAGAATTACA 
     1681 CCCGGGCCTA CTCAGAGGCT CTGGCCGCCT TTGGGAATGG GGCGCTGTTT GTGGAGAAGT 
     1741 TCATCGAGAA GCCACGGCAC ATCGAGGTGC AGATCTTGGG GGACCAGTAT GGGAACATCC 
     1801 TGCACCTGTA CGAGCGAGAC TGCTCCATCC AGCGGCGGCA CCAGAAGGTG GTCGAGATTG 
     1861 CCCCCGCCGC CCACCTGGAC CCGCAGCTTC GGACTCGGCT CACCAGCGAC TCTGTGAAAC 
     1921 TCGCTAAACA GGTGGGCTAC GAGAACGCAG GCACCGTGGA GTTCCTGGTG GACAGGCACG 
     1981 GCAAGCACTA CTTCATCGAG GTCAACTCCC GCCTGCAGGT GGAGCACACG GTCACAGAGG 
     2041 AGATCACCGA CGTAGACCTG GTCCATGCTC AGATCCACGT GGCTGAGGGC AGGAGCCTAC 
     2101 CCGACCTGGG CCTGCGGCAG GAGAACATCC GCATCAACGG GTGTGCCATC CAGTGCCGGG 
     2161 TCACCACCGA GGACCCCGCG CGCAGCTTCC AGCCGGACAC CGGCCGCATT GAGGTGTTCC 
     2221 GGAGCGGAGA GGGCATGGGC ATCCGCCTGG ATAATGCTTC CGCCTTCCAA GGAGCCGTCA 
     2281 TCTCGCCCCA CTACGACTCC CTGCTGGTCA AAGTCATTGC CCACGGCAAA GACCACCCCA 
     2341 CGGCCGCCAC CAAGATGAGC AGGGCCCTTG CGGAGTTCCG CGTCCGAGGT GTGAAGACCA 
     2401 ACATCGCCTT CCTGCAGAAT GTGCTCAACA ACCAGCAGTT CCTGGCAGGC ACTGTGGACA 
     2461 CCCAGTTCAT CGACGAGAAC CCAGAGCTGT TCCAGCTGCG GCCTGCACAG AACCGGGCCC 
     2521 AAAAGCTGTT GCACTACCTC GGCCATGTCA TGGTAAACGG TCCAACCACC CCGATTCCCG 
     2581 TCAAGGCCAG CCCCAGCCCC ACGGACCCCG TTGTCCCTGC AGTGCCCATA GGCCCGCCCC 
     2641 CGGCTGGTTT CAGAGACATC CTGCTGCGAG AGGGGCCTGA GGGCTTTGCT CGAGCTGTGC 
     2701 GGAACCACCC GGGGCTGCTG CTGATGGACA CGACCTTCAG GGACGCCCAC CAGTCACTGC 
     2761 TGGCCACTCG TGTGCGCACC CACGATCTCA AAAAGATCGC CCCCTATGTT GCCCACAACT 
     2821 TCAGCAAGCT CTTCAGCATG GAGAACTGGG GAGGAGCCAC GTTTGACGTC GCCATGCGCT 
     2881 TCCTGTATGA GTGCCCCTGG CGGCGGCTGC AGGAGCTCCG GGAGCTCATC CCCAACATCC 
     2941 CTTTCCAGAT GCTGCTGCGG GGGGCCAATG CTGTGGGCTA CACCAACTAC CCAGACAACG 
     3001 TGGTCTTCAA GTTCTGTGAA GTGGCCAAAG AGAATGGCAT GGATGTCTTC CGTGTGTTTG 
     3061 ACTCCCTCAA CTACTTGCCC AACATGCTGC TGGGCATGGA GGCGGCAGGA AGTGCCGGAG 
     3121 GCGTGGTGGA GGCTGCCATC TCATACACGG GCGACGTGGC CGACCCCAGC CGCACCAAGT 
     3181 ACTCACTGCA GTACTACATG GGCTTGGCCG AAGAGCTGGT GCGAGCTGGC ACCCACATCC 
     3241 TGTGCATCAA GGACATGGCC GGGCTGCTGA AGCCCACGGC CTGCACCATG CTGGTCAGCT 
     3301 CCCTCCGGGA CCGCTTCCCC GACCTCCCAC TGCACATCCA CACCCACGAC ACGTCAGGGG 
     3361 CAGGCGTGGC AGCCATGCTG GCCTGTGCCC AGGCTGGAGC TGATGTGGTG GATGTGGCAG 
     3421 CTGATTCCAT GTCTGGGATG ACTTCACAGC CCAGCATGGG GGCCCTGGTG GCCTGTACCA 
     3481 GAGGGACTCC CCTGGACACA GAGGTGCCCA TGGAGCGCGT GTTTGACTAC AGTGAGTACT 
     3541 GGGAGGGGGC TCGGGGACTG TACGCGGCCT TCGACTGCAC GGCCACCATG AAGTCTGGCA 
     3601 ACTCGGACGT GTATGAAAAT GAGATCCCAG GGGGCCAGTA CACCAACCTG CACTTCCAGG 
     3661 CCCACAGCAT GGGGCTTGGC TCCAAGTTCA AGGAGGTCAA GAAGGCCTAT GTGGAGGCCA 
     3721 ACCAGATGCT GGGCGATCTC ATCAAGGTGA CGCCCTCCTC CAAGATCGTG GGGGACCTGG 
     3781 CCCAGTTTAT GGTGCAGAAT GGATTGAGCC GGGCAGAGGC CGAAGCTCAG GCGGAAGAGC 
     3841 TGTCCTTTCC CCGCTCCGTG GTGGAGTTCC TGCAGGGCTA CATCGGTGTC CCCCATGGGG 
     3901 GGTTCCCCGA ACCCTTTCGC TCTAAGGTAC TGAAGGACCT GCCAAGGGTG GAGGGGCGGC 
     3961 CTGGAGCCTC CCTCCCTCCC CTGGATCTGC AGGCACTGGA GAAGGAGCTG GTAGACCGGC 
     4021 ATGGGGAGGA GGTGACGCCG GAAGATGTGC TCTCAGCAGC TATGTACCCC GATGTGTTTG 
     4081 CCCACTTCAA GGACTTCACT GCCACCTTTG GCCCCCTGGA TAGCCTGAAT ACTCGCCTCT 
     4141 TCCTGCAGGG ACCCAAGATC GCAGAGGAGT TTGAGGTGGA GCTGGAGCGG GGCAAGACGC 
     4201 TGCACATCAA AGCCCTGGCC GTGAGCGACC TGAACCGGGC CGGCCAGAGG CAGGTCTTCT 
     4261 TTGAGCTCAA TGGGCAGCTG CGGTCCATCT TGGTCAAGGA CACCCAGGCC ATGAAGGAGA 
     4321 TGCACTTCCA CCCCAAGGCC CTAAAGGACG TGAAGGGCCA GATCGGGGCG CCCATGCCTG 
     4381 GGAAGGTGAT AGACATCAAA GTGGTGGCAG GGGCCAAGGT GGCCAAGGGC CAGCCCCTGT 
     4441 GTGTGCTCAG TGCCATGAAG ATGGAGACTG TGGTGACCTC ACCCATGGAG GGTACTGTCC 
     4501 GCAAGGTTCA TGTGACCAAG GACATGACAC TGGAAGGTGA CGACCTCATC CTGGAGATCG 
     4561 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4621 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4681 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4741 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4801 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4861 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4921 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4981 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5041 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5101 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5161 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5221 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5281 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5341 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5401 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5461 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5521 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5581 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5641 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5701 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5761 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5821 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5881 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5941 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6001 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6061 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6121 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6181 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6241 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6301 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6361 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6421 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6481 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6541 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6601 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6661 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6721 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6781 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6841 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6901 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6961 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7021 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7081 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7141 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7201 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7261 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7321 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7381 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7441 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7501 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7561 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7621 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7681 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7741 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7801 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7861 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7921 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7981 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8041 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8101 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8161 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8221 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8281 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8341 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8401 TTTAACGCGA ATTTT
//