LOCUS       RC221816                9204 bp    DNA     circular  SYN 
DEFINITION  PTPRT (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, T (PTPRT), transcript variant 2
ACCESSION   NM_007050
VERSION     NM_007050.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5361..5452 
                     /label="Myc-DDK"  
     misc_feature    5482..6067 
                     /label="PolyA signal"  
     misc_feature    5539..5558 
                     /label="Restriction Enzyme"  
     misc_feature    6343..7015 
                     /label="ColE1"  
     misc_feature    7445..8239 
                     /label="Kan/Neo"  
     misc_feature    8274..8576 
                     /label="SV40 ori"  
     misc_feature    8662..9139 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5343..5349 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5343 
                     /label="PTPRT"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCCTC GCCGCGCTCG CCCTCAGCCT GCTCCTGAGG CTGCAGCTGC 
     1081 CGCCACTGCC CGGCGCCCGG GCTCAGAGCG CCGCAGGTGG CTGTTCCTTT GATGAGCACT 
     1141 ACAGCAACTG TGGTTATAGT GTGGCTCTAG GGACCAATGG GTTCACCTGG GAGCAGATTA 
     1201 ACACATGGGA GAAACCAATG CTGGACCAGG CAGTGCCCAC AGGATCTTTC ATGATGGTGA 
     1261 ACAGCTCTGG GAGAGCCTCT GGCCAGAAGG CCCACCTTCT CCTGCCAACC CTGAAGGAGA 
     1321 ATGACACCCA CTGCATCGAC TTCCATTACT ACTTCTCCAG CCGTGACAGG TCCAGCCCAG 
     1381 GGGCCTTGAA CGTCTACGTG AAGGTGAATG GTGGCCCCCA AGGGAACCCT GTGTGGAATG 
     1441 TGTCCGGGGT CGTCACTGAG GGCTGGGTGA AGGCAGAGCT CGCCATCAGC ACTTTCTGGC 
     1501 CACATTTCTA TCAGGTGATA TTTGAATCCG TCTCATTGAA GGGTCATCCT GGCTACATCG 
     1561 CCGTGGACGA GGTCCGGGTC CTTGCTCATC CATGCAGAAA AGCACCTCAT TTTCTGCGAC 
     1621 TCCAAAACGT GGAGGTGAAT GTGGGGCAGA ATGCCACATT TCAGTGCATT GCTGGTGGGA 
     1681 AGTGGTCTCA GCATGACAAG CTTTGGCTCC AGCAATGGAA TGGCAGGGAC ACGGCCCTGA 
     1741 TGGTCACCCG TGTGGTCAAC CACAGGCGCT TCTCAGCCAC AGTCAGTGTG GCAGACACTG 
     1801 CCCAGCGGAG CGTCAGCAAG TACCGCTGTG TGATCCGCTC TGATGGTGGG TCTGGTGTGT 
     1861 CCAACTACGC GGAGCTGATC GTGAAAGAGC CTCCCACGCC CATTGCTCCC CCAGAGCTGC 
     1921 TGGCTGTGGG GGCCACATAC CTGTGGATCA AGCCAAATGC CAACTCCATC ATCGGGGATG 
     1981 GCCCCATCAT CCTGAAGGAA GTGGAATATC GCACCACCAC AGGCACGTGG GCAGAGACCC 
     2041 ACATAGTCGA CTCTCCCAAC TATAAGCTGT GGCATCTGGA CCCCGATGTT GAGTATGAGA 
     2101 TCCGAGTGCT CCTCACACGA CCAGGTGAGG GGGGTACGGG ACCGCCAGGG CCTCCCCTCA 
     2161 CCACCAGGAC CAAGTGTGCA GATCCGGTAC ATGGCCCACA GAACGTGGAA ATCGTAGACA 
     2221 TCAGAGCCCG GCAGCTGACC CTGCAGTGGG AGCCCTTCGG CTACGCGGTG ACCCGCTGCC 
     2281 ATAGCTACAA CCTCACCGTG CAGTACCAGT ATGTGTTCAA CCAGCAGCAG TACGAGGCCG 
     2341 AGGAGGTCAT CCAGACCTCC TCCCACTACA CCCTGCGAGG CCTGCGCCCC TTCATGACCA 
     2401 TCCGGCTGCG ACTCTTGCTG TCTAACCCCG AGGGCCGAAT GGAGAGCGAG GAGCTGGTGG 
     2461 TGCAGACTGA GGAAGACGTT CCAGGAGCTG TTCCTCTAGA ATCCATCCAA GGGGGGCCCT 
     2521 TTGAGGAGAA GATCTACATC CAGTGGAAAC CTCCCAATGA GACCAATGGG GTCATCACGC 
     2581 TCTACGAGAT CAACTACAAG GCTGTCGGCT CGCTGGACCC AAGTGCTGAC CTCTCGAGCC 
     2641 AGAGGGGGAA AGTGTTCAAG CTCCGGAATG AAACCCACCA CCTCTTTGTG GGTCTGTACC 
     2701 CAGGGACCAC CTATTCCTTC ACCATCAAGG CCAGCACAGC AAAGGGCTTT GGGCCCCCTG 
     2761 TCACCACTCG GATTGCCACC AAAATTTCAG CTCCATCCAT GCCTGAGTAC GACACAGACA 
     2821 CCCCATTGAA TGAGACAGAC ACGACCATCA CAGTGATGCT GAAACCCGCT CAGTCCCGGG 
     2881 GAGCTCCTGT CAGTGTTTAT CAGCTGGTTG TCAAGGAGGA GCGACTTCAG AAGTCACGGA 
     2941 GGGCAGCTGA CATTATTGAG TGCTTTTCGG TGCCCGTGAG CTATCGGAAT GCCTCCAGCC 
     3001 TCGATTCTCT ACACTACTTT GCTGCTGAGT TGAAGCCTGC CAACCTGCCT GTCACCCAGC 
     3061 CATTTACAGT GGGTGACAAT AAGACATACA ATGGCTACTG GAACCCTCCT CTCTCTCCCC 
     3121 TGAAAAGCTA CAGCATCTAC TTCCAGGCAC TCAGCAAAGC CAATGGAGAG ACCAAAATCA 
     3181 ACTGTGTTCG TCTGGCTACA AAAGGTGCCT CCACCCAGAA TTCTAACACT GTGGAGCCAG 
     3241 AGAAGCAGGT GGACAACACC GTGAAGATGG CTGGCGTGAT CGCTGGCCTC CTCATGTTCA 
     3301 TCATCATTCT CCTGGGCGTG ATGCTCACCA TCAAAAGGAG AAGAAATGCT TATTCCTACT 
     3361 CCTATTACTT GAAGCTGGCC AAGAAGCAGA AGGAGACCCA GAGTGGAGCC CAGAGGGAGA 
     3421 TGGGGCCTGT GGCCTCTGCC GACAAACCCA CCACCAAGCT CAGCGCCAGC CGCAATGATG 
     3481 AAGGCTTCTC TTCTAGTTCT CAGGACGTCA ACGGATTCAC AGATGGCAGC CGCGGGGAGC 
     3541 TTTCCCAGCC CACCCTCACG ATCCAGACTC ATCCCTACCG CACCTGTGAC CCTGTGGAGA 
     3601 TGAGCTACCC CCGGGACCAG TTCCAACCCG CCATCCGGGT GGCTGACTTG CTGCAGCACA 
     3661 TCACGCAGAT GAAGAGAGGC CAGGGCTACG GGTTCAAGGA GGAATACGAG GCCTTACCAG 
     3721 AGGGGCAGAC AGCTTCGTGG GACACAGCCA AGGAGGATGA AAACCGCAAT AAGAATCGAT 
     3781 ATGGGAACAT CATATCCTAC GACCATTCCC GGGTGAGGCT GCTGGTGCTG GATGGAGACC 
     3841 CGCACTCTGA CTACATCAAT GCCAACTACA TTGACGGATA CCATCGACCT CGGCACTACA 
     3901 TTGCGACTCA AGGTCCGATG CAGGAGACTG TAAAGGACTT TTGGAGAATG ATCTGGCAGG 
     3961 AGAACTCCGC CAGCATCGTC ATGGTCACAA ACCTGGTGGA AGTGGGCAGG GTGAAATGTG 
     4021 TGCGATACTG GCCAGATGAC ACGGAGGTCT ACGGAGACAT TAAAGTCACC CTGATTGAAA 
     4081 CAGAGCCCCT GGCAGAATAC GTCATACGCA CCTTCACAGT CCAGAAGAAA GGCTACCATG 
     4141 AGATCCGGGA GCTCCGCCTC TTCCACTTCA CCAGCTGGCC TGACCACGGC GTTCCCTGCT 
     4201 ATGCCACTGG CCTTCTGGGC TTCGTCCGCC AGGTCAAGTT CCTCAACCCC CCGGAAGCTG 
     4261 GGCCCATAGT GGTCCACTGC AGTGCTGGGG CTGGGCGGAC TGGCTGCTTC ATTGCCATTG 
     4321 ACACCATGCT TGACATGGCC GAGAATGAAG GGGTGGTGGA CATCTTCAAC TGCGTGCGTG 
     4381 AGCTCCGGGC CCAAAGGGTC AACCTGGTAC AGACAGAGGA GCAATATGTG TTTGTGCACG 
     4441 ATGCCATCCT GGAAGCGTGC CTCTGTGGCA ACACTGCCAT CCCTGTGTGT GAGTTCCGTT 
     4501 CTCTCTACTA CAATATCAGC AGGCTGGACC CCCAGACAAA CTCCAGCCAA ATCAAAGATG 
     4561 AATTTCAGAC CCTCAACATT GTGACACCCC GTGTGCGGCC CGAGGACTGC AGCATTGGGC 
     4621 TCCTGCCCCG GAACCATGAT AAGAATCGAA GTATGGACGT GCTGCCTCTG GACCGCTGCC 
     4681 TGCCCTTCCT TATCTCAGTG GACGGAGAAT CCAGCAATTA CATCAACGCA GCACTGATGG 
     4741 ATAGCCACAA GCAGCCTGCC GCCTTCGTGG TCACCCAGCA CCCTCTACCC AACACCGTGG 
     4801 CAGACTTCTG GAGGCTGGTG TTCGATTACA ACTGCTCCTC TGTGGTGATG CTGAATGAGA 
     4861 TGGACACTGC CCAGTTCTGT ATGCAGTACT GGCCTGAGAA GACCTCCGGG TGCTATGGGC 
     4921 CCATCCAGGT GGAGTTCGTC TCCGCAGACA TCGACGAGGA CATCATCCAC AGAATATTCC 
     4981 GCATCTGTAA CATGGCCCGG CCACAGGATG GTTATCGTAT AGTCCAGCAC CTCCAGTACA 
     5041 TTGGCTGGCC TGCCTACCGG GACACGCCCC CCTCCAAGCG CTCTCTGCTC AAAGTGGTCC 
     5101 GACGACTGGA GAAGTGGCAG GAGCAGTATG ACGGGAGGGA GGGACGTACT GTGGTCCACT 
     5161 GCCTAAATGG GGGAGGCCGT AGTGGAACCT TCTGTGCCAT CTGCAGTGTG TGTGAGATGA 
     5221 TCCAGCAGCA AAACATCATT GACGTGTTCC ACATCGTGAA AACACTGCGT AACAACAAAT 
     5281 CCAACATGGT GGAGACCCTG GAACAGTATA AATTTGTATA CGAGGTGGCA CTGGAATATT 
     5341 TAAGCTCCTT TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5401 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5461 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5521 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5581 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5641 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5701 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5761 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5821 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5881 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5941 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     6001 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     6061 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6121 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6181 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6241 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6301 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6361 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6421 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6481 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6541 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6601 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6661 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6721 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6781 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6841 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6901 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6961 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     7021 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     7081 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7141 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7201 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7261 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7321 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7381 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7441 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7501 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7561 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7621 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7681 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7741 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7801 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7861 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7921 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7981 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     8041 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     8101 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8161 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8221 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8281 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8341 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8401 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8461 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8521 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8581 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8641 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8701 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8761 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8821 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8881 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8941 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     9001 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     9061 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9121 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9181 TAACAAAAAT TTAACGCGAA TTTT
//