LOCUS       RC221779                7320 bp    DNA     circular  SYN 
DEFINITION  PCDHGB1 (Myc-DDK-tagged)-Human protocadherin gamma subfamily B, 1 (PCDHGB1), transcript variant 2
ACCESSION   NM_032095
VERSION     NM_032095.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3477..3568 
                     /label="Myc-DDK"  
     misc_feature    3598..4183 
                     /label="PolyA signal"  
     misc_feature    3655..3674 
                     /label="Restriction Enzyme"  
     misc_feature    4459..5131 
                     /label="ColE1"  
     misc_feature    5561..6355 
                     /label="Kan/Neo"  
     misc_feature    6390..6692 
                     /label="SV40 ori"  
     misc_feature    6778..7255 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    3459..3465 
                     /label="Restriction Enzyme"  
     misc_feature    1029..3459 
                     /label="PCDHGB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG CAGAGAGCCA GAGAAGCCGA AATGATGAAA AGTCAGGTAC 
     1081 TGTTTCCCTT CCTGCTGTCT TTGTTCTGCG GGGCCATCTC CCAGCAGATC CGATACACGA 
     1141 TTCCAGAGGA GCTAGCCAAC GGCTCACGGG TGGGGAAACT TGCCAAGGAT CTGGGGCTCA 
     1201 GTGTCCGGGA GTTGCCAACT CGAAAACTGC GGGTTAGTGC AGAGGATTAT TTCAACGTTA 
     1261 GTTTGGAGAG CGGGGATTTG TTAGTGAACG GTAGGATAGA TCGAGAGAAG ATTTGCGGAA 
     1321 GGAAACTTGA GTGTGCACTA GAATTCGAAA CGGTCGCTGA AAACCCAATG AATGTTTTCC 
     1381 ACGTGGTTGT TGTAATCCAA GATATTAATG ACAATGCACC ACGTTTCGTT GCAAAAGGCA 
     1441 TTGACTTAGA AATTTGTGAG TCAGCCTTAC CCGGGGTAAA ATTCTCTCTG GATTCTGCTC 
     1501 AAGATGCAGA TGTGGAAGGC AATTCACTGA AGTTATACAC CATCAACCCC AATCAATACT 
     1561 TCTCTCTGTC AACGAAGGAA AGTCCTGATG GAAGTAAATA TCCGGTATTA CTGCTGGAAA 
     1621 AACCTCTAGA CAGGGAACAT CAGAGCTCTC ATCGCTTAAT CCTGACTGCC ATGGATGGCG 
     1681 GGGACCCGCC TCTAAGCGGC ACCACCCATA TCTGGATCCG AGTTACGGAT GCCAATGATA 
     1741 ATGCTCCCGT GTTTAGCCAG GAGGTATACA GGGTTAGCCT CCAAGAAAAC GTACCGTGGG 
     1801 GAACCTCCGT GCTGCGGGTG ATGGCCACAG ACCAGGATGA GGGCATTAAT GCAGAGATCA 
     1861 CCTATGCCTT CCTCAATTCC CCAATAAGTA CCAGCCTCTT CAATCTCAAT CCAAATACTG 
     1921 GCGACATCAC AACCAATGGC ACATTGGATT TTGAAGAGAC AAGTAGATAT GTGTTGAGTG 
     1981 TGGAAGCTAA GGATGGAGGA GTACACACAG CTCACTGTAA TGTTCAAATA GAAATTGTTG 
     2041 ACGAGAATGA CAATGCCCCA GAGGTGACAT TCATGTCCTT CTCTAACCAG ATTCCAGAGG 
     2101 ATTCAGACCT TGGAACTGTA ATAGCCCTCA TAAAAGTGCG AGACAAGGAT TCTGGGCAAA 
     2161 ATGGCATGGT GACATGCTAT ACTCAGGAAG AAGTTCCTTT CAAATTAGAA TCCACCTCGA 
     2221 AGAATTATTA CAAGCTGGTG ATTGCTGGAG CCCTAAACCG GGAGCAGACA GCAGACTACA 
     2281 ACGTCACAAT CATAGCCACC GACAAGGGCA AACCAGCCCT TTCCTCCAGG ACAAGCATCA 
     2341 CCCTGCACAT CTCCGACATC AACGACAATG CACCTGTTTT CCATCAGGCC TCCTATGTGG 
     2401 TCCACGTGTC TGAGAACAAC CCACCTGGCG CCTCCATTGC ACAAGTAAGC GCCTCCGACC 
     2461 CGGATTTGGG ACCCAACGGC AGAGTCTCCT ACTCTATTCT GGCCAGTGAC CTGGAGCCGC 
     2521 GGGAGCTGTT GTCCTACGTG TCCGTGAGCC CGCAGAGCGG GGTGGTGTTC GCGCAGCGCG 
     2581 CCTTCGACCA CGAGCAGCTG CGCGCCTTCG AGCTCACACT GCAGGCCAGG GACCAGGGCT 
     2641 CCCCCGCGCT CAGCGCCAAC GTGAGCCTGC GCGTGTTGGT GGGCGACCTC AATGACAATG 
     2701 CGCCACGGGT GCTGTACCCC GCGCTGGGGC CTGATGGCTC CGCCCTCTTC GATATGGTGC 
     2761 CACGCGCCGC AGAGCCCGGC TACCTGGTGA CCAAGGTGGT GGCGGTGGAC GCAGACTCAG 
     2821 GACACAACGC TTGGCTGTCC TACCACGTGC TGCAGGCCAG CGAGCCCGGG CTCTTCAGCC 
     2881 TGGGGTTGCG CACGGGTGAG GTGCGCACAG CGCGTGCCTT GGGCGACAGG GACGCGGCCC 
     2941 GCCAGCGCCT GCTGGTCGCT GTGCGTGATG GAGGACAGCC GCCACTCTCC GCCACCGCCA 
     3001 CGCTGCACCT AATCTTCGCG GATAGCCTGC AAGAGGTATT GCCAGACCTC AGCGACCGCC 
     3061 CTGAGCCCTC TGACCCCCAG ACGGAACTGC AGTTTTACCT GGTTGTGGCC TTGGCCTTGA 
     3121 TCTCAGTGCT CTTTCTCCTC GCGGTGATTC TAGCGATCGC CCTGCGCCTG CGACGTTCCT 
     3181 CCAGCCTCGA CACTGAGGGC TGCTTTCAAA CCGGTCTCTG CTCCAAGTCT GGGCCCGGGG 
     3241 TTCCTCCCAA CCACAGCGAG GGGACTTTGC CCTATTCCTA CAATCTATGT ATTGCCTCTC 
     3301 ATTCTGCAAA GACAGAGTTT AATTCTCTCA ACCTGACACC GGAAATGGCT CCCCCTCAGG 
     3361 ATCTGCTGTG TGATGATCCT TCTATGGTTG TATGTGCCAG TAATGAAGAT CACAAAATCG 
     3421 CTTATGACCC TTCTTTGTCT TCGCACGTGA GTTTCTGCAA ATCTAGTACG CGTACGCGGC 
     3481 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3541 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3601 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3661 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3721 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3781 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3841 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3901 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3961 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4021 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4081 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4141 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4201 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4261 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4321 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4381 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4441 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4501 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4561 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4621 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4681 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4741 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4801 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4861 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4921 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4981 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5041 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5101 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5161 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5221 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5281 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5341 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5401 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5461 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5521 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5581 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5641 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5701 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5761 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5821 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5881 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5941 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6001 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6061 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6121 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6181 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6241 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6301 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6361 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6421 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6481 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6541 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6601 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6661 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6721 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6781 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6841 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6901 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6961 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7021 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7081 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7141 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7201 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7261 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//