LOCUS       RC221766                9036 bp    DNA     circular  SYN 
DEFINITION  EIF3A (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 3, subunit A (EIF3A)
ACCESSION   NM_003750
VERSION     NM_003750.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5191..5282 
                     /label="Myc-DDK"  
     misc_feature    5312..5897 
                     /label="PolyA signal"  
     misc_feature    5369..5388 
                     /label="Restriction Enzyme"  
     misc_feature    6173..6845 
                     /label="ColE1"  
     misc_feature    7275..8069 
                     /label="Kan/Neo"  
     misc_feature    8104..8406 
                     /label="SV40 ori"  
     misc_feature    8492..8969 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5166..5173 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5166 
                     /label="EIF3A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGCCTAT TTTCAGAGGC CGGAAAATGC CCTCAAACGC GCCAACGAAT 
     1081 TTCTTGAGGT TGGCAAAAAG CAGCCTGCTC TGGATGTTCT TTATGATGTT ATGAAAAGTA 
     1141 AAAAACATAG AACATGGCAA AAGATACACG AACCAATTAT GTTGAAATAC TTGGAACTTT 
     1201 GCGTGGATCT TCGCAAGAGC CACTTGGCAA AGGAGGGGTT ATACCAGTAT AAGAACATTT 
     1261 GTCAACAGGT GAACATAAAA TCTCTGGAGG ATGTTGTTAG GGCATATTTG AAAATGGCAG 
     1321 AGGAAAAAAC TGAAGCTGCT AAAGAAGAAT CTCAGCAGAT GGTCTTAGAT ATAGAGGATC 
     1381 TAGATAATAT TCAAACTCCT GAGAGTGTTC TCCTAAGTGC TGTAAGTGGT GAAGACACTC 
     1441 AGGATCGTAC TGACAGATTA CTTTTAACTC CATGGGTTAA ATTCCTGTGG GAGTCTTACA 
     1501 GGCAGTGTTT GGACCTTCTT AGAAACAATT CTAGAGTAGA GCGCCTGTAC CATGATATTG 
     1561 CCCAGCAAGC TTTCAAATTC TGCCTCCAAT ACACGCGTAA GGCTGAATTC CGTAAACTGT 
     1621 GTGACAATTT GAGAATGCAC TTATCGCAGA TTCAGCGCCA CCATAACCAA AGTACGGCAA 
     1681 TCAATCTTAA TAATCCAGAG AGCCAGTCCA TGCATTTGGA AACCAGACTT GTTCAGCTGG 
     1741 ACAGTGCTAT CAGCATGGAA TTGTGGCAGG AAGCATTCAA AGCTGTGGAA GATATTCACG 
     1801 GGCTATTCTC CTTGTCTAAA AAACCACCTA AACCTCAGTT GATGGCAAAT TACTATAACA 
     1861 AAGTCTCAAC TGTGTTTTGG AAATCTGGAA ATGCTCTTTT TCATGCATCT ACACTCCATC 
     1921 GTCTTTACCA TCTCTCTAGA GAAATGAGAA AGAATCTCAC ACAAGACGAG ATGCAAAGAA 
     1981 TGTCTACTAG AGTCCTTTTA GCCACTCTTT CCATCCCTAT TACTCCTGAG CGTACGGATA 
     2041 TTGCTCGACT TCTGGATATG GATGGCATTA TAGTTGAAAA ACAGCGTCGC CTTGCAACAC 
     2101 TACTAGGTCT TCAAGCCCCA CCGACACGAA TTGGCCTTAT TAATGATATG GTCAGATTTA 
     2161 ATGTACTACA ATATGTTGTC CCAGAAGTGA AAGACCTTTA CAATTGGCTT GAAGTAGAAT 
     2221 TTAACCCATT AAAACTCTGT GAGCGAGTCA CAAAGGTTCT AAATTGGGTT AGGGAACAAC 
     2281 CTGAAAAGGA ACCGGAATTG CAGCAGTATG TGCCACAACT GCAAAACAAC ACCATCCTCC 
     2341 GCCTTCTGCA GCAGGTGTCA CAGATTTATC AGAGCATTGA GTTTTCTCGT TTGACTTCTT 
     2401 TGGTTCCTTT TGTTGATGCT TTCCAACTGG AACGGGCCAT AGTAGATGCA GCCAGGCATT 
     2461 GCGACTTGCA GGTTCGTATT GATCACACTT CTCGGACCCT GAGTTTTGGA TCTGATTTGA 
     2521 ATTATGCTAC TCGAGAAGAT GCTCCGATTG GTCCTCATTT GCAAAGCATG CCTTCAGAGC 
     2581 AGATAAGAAA CCAGCTGACA GCCATGTCCT CAGTACTTGC AAAAGCACTT GAAGTCATTA 
     2641 AACCAGCTCA TATACTGCAA GAGAAAGAAG AACAGCATCA GTTGGCTGTC ACTGCATACC 
     2701 TTAAAAATTC ACGAAAAGAG CACCAGCGGA TCCTGGCTCG CCGCCAGACA ATTGAGGAGA 
     2761 GAAAAGAGCG CCTTGAGAGT CTGAATATTC AGCGTGAGAA AGAAGAATTG GAACAGAGGG 
     2821 AAGCTGAACT CCAGAAAGTG CGGAAGGCTG AGGAAGAGAG GCTGCGCCAG GAAGCAAAGG 
     2881 AGAGAGAGAA GGAGCGTATC TTACAGGAAC ATGAACAAAT CAAAAAGAAA ACTGTCCGAG 
     2941 AGCGTTTGGA GCAGATCAAG AAAACAGAAC TGGGTGCCAA AGCATTCAAA GATATTGATA 
     3001 TTGAAGACCT TGAGGAATTG GATCCAGATT TTATCATGGC TAAACAGGTT GAACAACTGG 
     3061 AGAAAGAAAA GAAAGAACTT CAAGAACGCC TAAAGAATCA AGAAAAGAAG ATTGACTATT 
     3121 TTGAAAGAGC CAAACGTTTG GAAGAAATTC CTTTGATAAA GAGCGCTTAC GAGGAACAGA 
     3181 GAATTAAAGA CATGGATCTG TGGGAGCAAC AAGAGGAAGA AAGAATTACT ACAATGCAGC 
     3241 TAGAACGTGA AAAGGCTCTT GAACATAAGA ATCGAATGTC ACGAATGCTT GAAGACAGAG 
     3301 ATTTATTCGT AATGCGACTC AAAGCTGCAC GGCAGTCTGT TTATGAGGAA AAACTTAAAC 
     3361 AGTTTGAAGA GCGATTAGCA GAAGAAAGGC ATAATCGATT GGAAGAACGG AAAAGGCAGC 
     3421 GTAAAGAAGA ACGCAGGATA ACATACTATA GAGAAAAAGA AGAGGAGGAG CAGAGAAGGG 
     3481 CAGAAGAACA AATGCTAAAA GAGCGGGAAG AGAGAGAGCG CGCCGAACGA GCAAAACGCG 
     3541 AGGAAGAGCT ACGAGAGTAT CAGGAGCGGG TGAAGAAATT AGAAGAAGTG GAAAGGAAAA 
     3601 AACGCCAAAG GGAGTTGGAA ATTGAAGAAC GAGAACGGCG TAGAGAGGAA GAGAGAAGAC 
     3661 TTGGCGATAG TTCCCTTTCT AGAAAGGACT CTCGTTGGGG AGATAGAGAT TCAGAAGGCA 
     3721 CCTGGAGAAA AGGACCTGAA GCAGATTCTG AGTGGAGAAG AGGCCCGCCA GAGAAGGAGT 
     3781 GGAGACGTGG AGAAGGGCGA GATGAGGACA GGTCTCATAG AAGAGATGAA GAGCGGCCCC 
     3841 GGCGTCTGGG GGATGATGAA GATAGAGAGC CCTCTCTTAG ACCAGACGAT GATCGGGTTC 
     3901 CCCGGCGTGG CATGGATGAT GACAGAGGCC CTAGACGTGG TCCTGAGGAA GATAGGTTCT 
     3961 CTCGTCGTGG GGCAGACGAT GACCGGCCTT CCTGGCGTAA CACAGATGAT GACAGGCCTC 
     4021 CCAGACGAAT TGCCGATGAA GACAGGGGAA ACTGGCGTCA TGCGGATGAT GACAGACCAC 
     4081 CTAGACGAGG ACTGGATGAG GACAGAGGAA GCTGGCGAAC AGCTGATGAG GACAGAGGAC 
     4141 CAAGACGTGG GATGGATGAT GACCGGGGGC CGAGGCGAGG AGGCGCTGAT GATGAGCGAT 
     4201 CATCCTGGCG TAATGCTGAT GATGACCGGG GTCCCAGGCG AGGGTTGGAT GATGATCGGG 
     4261 GTCCCAGGCG AGGCATGGAT GATGACCGGG GTCCCAGGCG AGGCATGGAT GATGACCGGG 
     4321 GTCCCAGGCG AGGCATGGAT GATGACCGGG GTCCCAGGCG AGGGTTGGAT GATGATCGAG 
     4381 GACCTTGGAG GAACGCCGAT GATGACAGAA TTCCCAGGCG TGGTGCAGAG GATGACAGGG 
     4441 GCCCTTGGAG AAACATGGAT GATGATCGCC TTTCAAGACG TGCTGATGAT GATCGGTTTC 
     4501 CCAGACGGGG TGATGACTCA AGACCTGGTC CTTGGAGACC ATTAGTCAAG CCAGGTGGAT 
     4561 GGAGAGAGAA AGAAAAAGCC AGAGAGGAGA GCTGGGGTCC ACCTCGAGAA TCAAGGCCAT 
     4621 CAGAAGAACG TGAATGGGAC AGAGAAAAAG AAAGGGACAG AGATAATCAA GATCGGGAGG 
     4681 AGAATGACAA GGACCCTGAG AGAGAAAGGG ACAGAGAGAG AGATGTGGAT CGAGAGGATC 
     4741 GCTTCAGAAG ACCTAGGGAT GAAGGTGGCT GGAGAAGAGG ACCAGCTGAG GAATCTTCAA 
     4801 GCTGGAGAGA CTCAAGTCGC CGGGACGATA GGGATAGGGA TGACCGTCGC CGTGAGAGGG 
     4861 ATGACCGGCG TGATCTAAGA GAAAGACGAG ATCTAAGAGA CGACAGGGAC CGAAGAGGAC 
     4921 CTCCACTCAG ATCAGAACGT GAAGAAGTAA GTTCTTGGAG ACGTGCTGAT GACAGGAAAG 
     4981 ATGACCGGGT GGAAGAGCGG GACCCTCCTC GTCGAGTTCC TCCCCCAGCT CTTTCAAGAG 
     5041 ACCGAGAAAG AGACCGAGAC CGAGAAAGAG AAGGTGAAAA AGAGAAGGCC TCATGGAGAG 
     5101 CTGAGAAAGA TAGGGAATCT CTCCGTCGTA CTAAAAATGA GACTGATGAA GATGGATGGA 
     5161 CCACAGTACG ACGTAGCGGA CCGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5221 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5281 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5341 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5401 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5461 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5521 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5581 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5641 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5701 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5761 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5821 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5881 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5941 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6001 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6061 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6121 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6181 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6241 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6301 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6361 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6421 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6481 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6541 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6601 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6661 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6721 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6781 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6841 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6901 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6961 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7021 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7081 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7141 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7201 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7261 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7321 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7381 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7441 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7501 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7561 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7621 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7681 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7741 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7801 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7861 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7921 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7981 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8041 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8101 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8161 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8221 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8281 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8341 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8401 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8461 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8521 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8581 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8641 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8701 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8761 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8821 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8881 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8941 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9001 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//