LOCUS       RC221644                9135 bp    DNA     circular  SYN 
DEFINITION  COL2A1 (Myc-DDK-tagged)-Human collagen, type II, alpha 1 (COL2A1), transcript variant 2
ACCESSION   NM_033150
VERSION     NM_033150.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5292..5383 
                     /label="Myc-DDK"  
     misc_feature    5413..5998 
                     /label="PolyA signal"  
     misc_feature    5470..5489 
                     /label="Restriction Enzyme"  
     misc_feature    6274..6946 
                     /label="ColE1"  
     misc_feature    7376..8170 
                     /label="Kan/Neo"  
     misc_feature    8205..8507 
                     /label="SV40 ori"  
     misc_feature    8593..9070 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5274..5280 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5274 
                     /label="COL2A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATTCGCCTC GGGGCTCCCC AGACGCTGGT GCTGCTGACG CTGCTCGTCG 
     1081 CCGCTGTCCT TCGGTGTCAG GGCCAGGATG TCCGGCAACC AGGACCAAAG GGACAGAAAG 
     1141 GAGAACCTGG AGACATCAAG GATATTGTAG GACCCAAAGG ACCTCCTGGG CCTCAGGGAC 
     1201 CTGCAGGGGA ACAAGGACCC AGAGGGGATC GTGGTGACAA AGGTGAAAAA GGTGCCCCTG 
     1261 GACCTCGTGG CAGAGATGGA GAACCTGGGA CCCCTGGAAA TCCTGGCCCC CCTGGTCCTC 
     1321 CCGGCCCCCC TGGTCCCCCT GGTCTTGGTG GAAACTTTGC TGCCCAGATG GCTGGAGGAT 
     1381 TTGATGAAAA GGCTGGTGGC GCCCAGTTGG GAGTAATGCA AGGACCAATG GGCCCCATGG 
     1441 GACCTCGAGG ACCTCCAGGC CCTGCAGGTG CTCCTGGGCC TCAAGGATTT CAAGGCAATC 
     1501 CTGGTGAACC TGGTGAACCT GGTGTCTCTG GTCCCATGGG TCCCCGTGGT CCTCCTGGTC 
     1561 CCCCTGGAAA GCCTGGTGAT GATGGTGAAG CTGGAAAACC TGGAAAAGCT GGTGAAAGGG 
     1621 GTCCGCCTGG TCCTCAGGGT GCTCGTGGTT TCCCAGGAAC CCCAGGCCTT CCTGGTGTCA 
     1681 AAGGTCACAG AGGTTATCCA GGCCTGGACG GTGCTAAGGG AGAGGCGGGT GCTCCTGGTG 
     1741 TGAAGGGTGA GAGTGGTTCC CCGGGTGAGA ACGGATCTCC GGGCCCAATG GGTCCTCGTG 
     1801 GCCTGCCTGG TGAAAGAGGA CGGACTGGCC CTGCTGGCGC TGCGGGTGCC CGAGGCAACG 
     1861 ATGGTCAGCC AGGCCCCGCA GGGCCTCCGG GTCCTGTCGG TCCTGCTGGT GGTCCTGGCT 
     1921 TCCCTGGTGC TCCTGGAGCC AAGGGTGAAG CCGGCCCCAC TGGTGCCCGT GGTCCTGAAG 
     1981 GTGCTCAAGG TCCTCGCGGT GAACCTGGTA CTCCTGGGTC CCCTGGGCCT GCTGGTGCCT 
     2041 CCGGTAACCC TGGAACAGAT GGAATTCCTG GAGCCAAAGG ATCTGCTGGT GCTCCTGGCA 
     2101 TTGCTGGTGC TCCTGGCTTC CCTGGGCCAC GGGGCCCTCC TGGCCCTCAA GGTGCAACTG 
     2161 GTCCTCTGGG CCCGAAAGGT CAGACGGGTG AACCTGGTAT TGCTGGCTTC AAAGGTGAAC 
     2221 AAGGCCCCAA GGGAGAACCT GGCCCTGCTG GCCCCCAGGG AGCCCCTGGA CCCGCTGGTG 
     2281 AAGAAGGCAA GAGAGGTGCC CGTGGAGAGC CTGGTGGCGT TGGGCCCATC GGTCCCCCTG 
     2341 GAGAAAGAGG TGCTCCCGGC AACCGCGGTT TCCCAGGTCA AGATGGTCTG GCAGGTCCCA 
     2401 AGGGAGCCCC TGGAGAGCGA GGGCCCAGTG GTCTTGCTGG CCCCAAGGGA GCCAACGGTG 
     2461 ACCCTGGCCG TCCTGGAGAA CCTGGCCTTC CTGGAGCCCG GGGTCTCACT GGCCGCCCTG 
     2521 GTGATGCTGG TCCTCAAGGC AAAGTTGGCC CTTCTGGAGC CCCTGGTGAA GATGGTCGTC 
     2581 CTGGACCTCC AGGTCCTCAG GGGGCTCGTG GGCAGCCTGG TGTCATGGGT TTCCCTGGCC 
     2641 CCAAAGGTGC CAACGGTGAG CCTGGCAAAG CTGGTGAGAA GGGACTGCCT GGTGCTCCTG 
     2701 GTCTGAGGGG TCTTCCTGGC AAAGATGGTG AGACAGGTGC TGCAGGACCC CCTGGCCCTG 
     2761 CTGGACCTGC TGGTGAACGA GGCGAGCAGG GTGCTCCTGG GCCATCTGGG TTCCAGGGAC 
     2821 TTCCTGGCCC TCCTGGTCCC CCAGGTGAAG GTGGAAAACC AGGTGACCAG GGTGTTCCCG 
     2881 GTGAAGCTGG AGCCCCTGGC CTCGTGGGTC CCAGGGGTGA ACGAGGTTTC CCAGGTGAAC 
     2941 GTGGCTCTCC CGGTGCCCAG GGCCTCCAGG GTCCCCGTGG CCTCCCCGGC ACTCCTGGCA 
     3001 CTGATGGTCC CAAAGGTGCA TCTGGCCCAG CAGGCCCCCC TGGGGCTCAG GGCCCTCCAG 
     3061 GTCTTCAGGG AATGCCTGGC GAGAGGGGAG CAGCTGGTAT CGCTGGGCCC AAAGGCGACA 
     3121 GGGGTGACGT TGGTGAGAAA GGCCCTGAGG GAGCCCCTGG AAAGGATGGT GGACGAGGCC 
     3181 TGACAGGTCC CATTGGCCCC CCTGGCCCAG CTGGTGCTAA TGGCGAGAAG GGAGAAGTTG 
     3241 GACCTCCTGG TCCTGCAGGA AGTGCTGGTG CTCGTGGCGC TCCGGGTGAA CGTGGAGAGA 
     3301 CTGGGCCCCC CGGACCAGCG GGATTTGCTG GGCCTCCTGG TGCTGATGGC CAGCCTGGGG 
     3361 CCAAGGGTGA GCAAGGAGAG GCCGGCCAGA AAGGCGATGC TGGTGCCCCT GGTCCTCAGG 
     3421 GCCCCTCTGG AGCACCTGGG CCTCAGGGTC CTACTGGAGT GACTGGTCCT AAAGGAGCCC 
     3481 GAGGTGCCCA AGGCCCCCCG GGAGCCACTG GATTCCCTGG AGCTGCTGGC CGCGTTGGAC 
     3541 CCCCAGGCTC CAATGGCAAC CCTGGACCCC CTGGTCCCCC TGGTCCTTCT GGAAAAGATG 
     3601 GTCCCAAAGG TGCTCGAGGA GACAGCGGCC CCCCTGGCCG AGCTGGTGAA CCCGGCCTCC 
     3661 AAGGTCCTGC TGGACCCCCT GGCGAGAAGG GAGAGCCTGG AGATGACGGT CCCTCTGGTG 
     3721 CCGAAGGTCC ACCAGGTCCC CAGGGTCTGG CTGGTCAGAG AGGCATCGTC GGTCTGCCTG 
     3781 GGCAACGTGG TGAGAGAGGA TTCCCTGGCT TGCCTGGCCC GTCGGGTGAG CCCGGCAAGC 
     3841 AGGGTGCTCC TGGAGCATCT GGAGACAGAG GTCCTCCTGG CCCCGTGGGT CCTCCTGGCC 
     3901 TGACGGGTCC TGCAGGTGAA CCTGGACGAG AGGGAAGCCC CGGTGCTGAT GGCCCCCCTG 
     3961 GCAGAGATGG CGCTGCTGGA GTCAAGGGTG ATCGTGGTGA GACTGGTGCT GTGGGAGCTC 
     4021 CTGGAGCCCC TGGGCCCCCT GGCTCCCCTG GCCCCGCTGG TCCAACTGGC AAGCAAGGAG 
     4081 ACAGAGGAGA AGCTGGTGCA CAAGGCCCCA TGGGACCCTC AGGACCAGCT GGAGCCCGGG 
     4141 GAATCCAGGG TCCTCAAGGC CCCAGAGGTG ACAAAGGAGA GGCTGGAGAG CCTGGCGAGA 
     4201 GAGGCCTGAA GGGACACCGT GGCTTCACTG GTCTGCAGGG TCTGCCCGGC CCTCCTGGTC 
     4261 CTTCTGGAGA CCAAGGTGCT TCTGGTCCTG CTGGTCCTTC TGGCCCTAGA GGTCCTCCTG 
     4321 GCCCCGTCGG TCCCTCTGGC AAAGATGGTG CTAATGGAAT CCCTGGCCCC ATTGGGCCTC 
     4381 CTGGTCCCCG TGGACGATCA GGCGAAACCG GCCCTGCTGG TCCTCCTGGA AATCCTGGAC 
     4441 CCCCTGGTCC TCCAGGTCCC CCTGGCCCTG GCATCGACAT GTCCGCCTTT GCTGGCTTAG 
     4501 GCCCGAGAGA GAAGGGCCCC GACCCCCTGC AGTACATGCG GGCCGACCAG GCAGCCGGTG 
     4561 GCCTGAGACA GCATGACGCC GAGGTGGATG CCACACTCAA GTCCCTCAAC AACCAGATTG 
     4621 AGAGCATCCG CAGCCCCGAG GGCTCCCGCA AGAACCCTGC TCGCACCTGC AGAGACCTGA 
     4681 AACTCTGCCA CCCTGAGTGG AAGAGTGGAG ACTACTGGAT TGACCCCAAC CAAGGCTGCA 
     4741 CCTTGGACGC CATGAAGGTT TTCTGCAACA TGGAGACTGG CGAGACTTGC GTCTACCCCA 
     4801 ATCCAGCAAA CGTTCCCAAG AAGAACTGGT GGAGCAGCAA GAGCAAGGAG AAGAAACACA 
     4861 TCTGGTTTGG AGAAACCATC AATGGTGGCT TCCATTTCAG CTATGGAGAT GACAATCTGG 
     4921 CTCCCAACAC TGCCAACGTC CAGATGACCT TCCTACGCCT GCTGTCCACG GAAGGCTCCC 
     4981 AGAACATCAC CTACCACTGC AAGAACAGCA TTGCCTATCT GGACGAAGCA GCTGGCAACC 
     5041 TCAAGAAGGC CCTGCTCATC CAGGGCTCCA ATGACGTGGA GATCCGGGCA GAGGGCAATA 
     5101 GCAGGTTCAC GTACACTGCC CTGAAGGATG GCTGCACGAA ACATACCGGT AAGTGGGGCA 
     5161 AGACTGTTAT CGAGTACCGG TCACAGAAGA CCTCACGCCT CCCCATCATT GACATTGCAC 
     5221 CCATGGACAT AGGAGGGCCC GAGCAGGAAT TCGGTGTGGA CATAGGGCCG GTCTGCTTCT 
     5281 TGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5341 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5401 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5461 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5521 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5581 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5641 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5701 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5761 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5821 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5881 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5941 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6001 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6061 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6121 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6181 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6241 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6301 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6361 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6421 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6481 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6541 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6601 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6661 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6721 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6781 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6841 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6901 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6961 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7021 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7081 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7141 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7201 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7261 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7321 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7381 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7441 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7501 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7561 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7621 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7681 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7741 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7801 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7861 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7921 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7981 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8041 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8101 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8161 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8221 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8281 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8341 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8401 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8461 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8521 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8581 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8641 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8701 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8761 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8821 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8881 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8941 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9001 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9061 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9121 TTTAACGCGA ATTTT
//