LOCUS       RC221621                8559 bp    DNA     circular  SYN 
DEFINITION  AFF3 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 3 (AFF3), transcript variant 1
ACCESSION   NM_002285
VERSION     NM_002285.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4716..4807 
                     /label="Myc-DDK"  
     misc_feature    4837..5422 
                     /label="PolyA signal"  
     misc_feature    4894..4913 
                     /label="Restriction Enzyme"  
     misc_feature    5698..6370 
                     /label="ColE1"  
     misc_feature    6800..7594 
                     /label="Kan/Neo"  
     misc_feature    7629..7931 
                     /label="SV40 ori"  
     misc_feature    8017..8494 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4698..4704 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4698 
                     /label="AFF3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAGCTTC GACTTAGCCC TGCTCCAGGA ATGGGACCTC GAGTCACTGT 
     1081 GTGTCTATGA ACCAGATAGA AATGCATTAC GGAGGAAAGA ACGAGAAAGA AGAAATCAAG 
     1141 AAACTCAACA GGATGATGGC ACGTTTAATT CTAGTTACTC TCTCTTCAGT GAGCCCTACA 
     1201 AGACTAACAA GGGGGATGAA CTCTCCAACC GGATCCAGAA CACTTTAGGC AATTATGATG 
     1261 AAATGAAAGA CTTTTTAACT GATAGATCCA ATCAGAGTCA TCTCGTTGGA GTTCCCAAAC 
     1321 CTGGGGTTCC TCAGACTCCT GTGAACAAGA TCGATGAACA TTTTGTTGCA GATTCAAGAG 
     1381 CCCAGAACCA GCCCTCGTCT ATCTGTAGCA CTACAACTTC CACACCAGCA GCTGTCCCCG 
     1441 TGCAGCAGAG TAAGAGAGGC ACTATGGGCT GGCAGAAGGC TGGGCACCCA CCCTCTGACG 
     1501 GCCAACAGAG AGCAACACAA CAGGGCTCTC TCAGGACCTT GCTTGGAGAT GGTGTTGGCA 
     1561 GACAGCAGCC TCGGGCCAAA CAAGTGTGCA ATGTGGAGGT GGGCCTTCAG ACCCAGGAGA 
     1621 GGCCACCTGC CATGGCGGCC AAGCACAGCA GCAGCGGACA CTGTGTTCAG AACTTTCCTC 
     1681 CATCCCTAGC TTCAAAACCC AGCCTGGTCC AGCAGAAACC GACCGCGTAT GTGAGGCCAA 
     1741 TGGACGGCCA AGATCAGGCC CCTGATGAGT CTCCTAAGCT GAAGTCGTCT TCGGAAACCA 
     1801 GCGTGCACTG CACATCATAC AGGGGAGTCC CTGCCAGCAA GCCGGAGCCT GCCAGAGCCA 
     1861 AGGCCAAGCT CTCCAAGTTC AGCATCCCCA AGCAGGGGGA GGAGAGTAGA TCTGGAGAAA 
     1921 CCAACAGCTG TGTTGAAGAA ATAATCCGGG AGATGACCTG GCTTCCACCA CTTTCTGCTA 
     1981 TTCAAGCACC TGGCAAAGTG GAACCAACCA AATTTCCATT TCCAAATAAG GACTCTCAGC 
     2041 TTGTATCCTC TGGACACAAT AATCCAAAGA AAGGTGATGC AGAGCCAGAG AGTCCAGACA 
     2101 ATGGCACATC GAATACATCA ATGCTGGAAG ATGACCTTAA GCTAAGCAGT GATGAAGAGG 
     2161 AGAATGAACA GCAGGCAGCT CAGAGAACGG CTCTCCGCGC TCTCTCTGAC AGCGCCGTGG 
     2221 TCCAGCAGCC CAACTGCAGA ACCTCGGTGC CTTCCAGCAA GGGCAGCAGC AGCAGCAGCA 
     2281 GCAGCGGCAG CAGCAGCTCC TCCAGCGACT CAGAGAGCAG CTCCGGATCT GACTCGGAGA 
     2341 CCGAGAGCAG CTCCAGCGAG AGTGAGGGCA GCAAGCCCCC CCACTTCTCC AGCCCCGAGG 
     2401 CTGAACCGGC ATCCTCTAAC AAGTGGCAGC TGGATAAATG GCTAAACAAA GTTAATCCCC 
     2461 ACAAGCCTCC TATTCTGATC CAAAATGAAA GCCACGGGTC AGAGAGCAAT CAGTACTACA 
     2521 ACCCGGTGAA AGAGGACGTC CAGGACTGTG GGAAAGTCCC CGACGTTTGC CAGCCCAGCC 
     2581 TGAGAGAGAA GGAGATCAAG AGCACTTGCA AGGAGGAGCA AAGGCCAAGG ACAGCCAACA 
     2641 AGGCCCCTGG GAGTAAAGGC GTGAAGCAGA AGTCCCCGCC CGCGGCCGTG GCCGTGGCGG 
     2701 TGAGCGCAGC CGCCCCGCCA CCCGCAGTGC CCTGTGCGCC CGCGGAGAAC GCGCCCGCGC 
     2761 CTGCCCGGAG GTCCGCGGGC AAGAAGCCCA CCAGGCGCAC CGAGAGGACC TCAGCCGGGG 
     2821 ACGGCGCCAA CTGCCACCGG CCCGAGGAGC CCGCGGCCGC GGACGCGCTG GGGACGAGCG 
     2881 TGGTGGTCCC CCCGGAGCCC ACCAAAACCA GGCCCTGTGG CAACAACAGA GCGAGCCACC 
     2941 GCAAGGAGCT GCGCTCCTCC GTGACCTGCG AGAAGCGCCG CACGCGGGGG CTAAGCAGGA 
     3001 TCGTCCCCAA ATCCAAGGAG TTCATTGAGA CAGAGTCGTC ATCTTCATCC TCCTCCTCGG 
     3061 ACTCCGACCT GGAGTCCGAG CAGGAGGAGT ACCCTCTGTC CAAAGCACAG ACCGTGGCTG 
     3121 CCTCTGCCTC CTCCGGGAAT GATCAGAGGC TGAAGGAGGC CGCTGCCAAC GGGGGCAGTG 
     3181 GTCCTAGGGC CCCTGTAGGC TCCATCAACG CCAGGACCAC CAGTGACATC GCCAAGGAGC 
     3241 TGGAGGAGCA GTTCTACACA CTGGTCCCCT TTGGCCGGAA CGAACTTCTC TCCCCTCTAA 
     3301 AGGACAGTGA TGAGATCAGG TCTCTCTGGG TCAAAATCGA CCTGACCCTC CTGTCCAGGA 
     3361 TCCCAGAACA CCTGCCCCAG GAGCCAGGGG TATTGAGCGC CCCTGCCACC AAGGACTCTG 
     3421 AGAGCGCACC GCCCAGCCAC ACCTCGGACA CACCTGCAGA AAAGGCTTTG CCAAAATCCA 
     3481 AGAGGAAACG CAAGTGTGAC AACGAAGACG ACTACAGGGA GATCAAGAAG TCCCAGGGAG 
     3541 AGAAAGACAG CTCTTCAAGA CTGGCCACCT CCACCAGTAA TACTTTGTCT GCAAACCACT 
     3601 GCAACATGAA CATCAACAGT GTGGCAATAC CAATAAATAA AAATGAAAAA ATGCTTCGGT 
     3661 CGCCCATCTC ACCCCTCTCT GATGCATCTA AACACAAATA CACCAGCGAG GACTTAACTT 
     3721 CTTCCAGCCG ACCTAATGGC AACAGTTTGT TTACTTCAGC CTCTTCCAGC AAAAAGCCTA 
     3781 AGGCCGACAG CCAGCTGCAG CCTCACGGCG GAGACCTCAC GAAAGCAGCT CACAACAATT 
     3841 CTGAAAACAT TCCCCTCCAC AAGTCACGGC CGCAGACGAA GCCGTGGTCT CCAGGCTCCA 
     3901 ACGGCCACAG GGACTGCAAG AGGCAGAAAC TTGTCTTCGA TGATATGCCT CGCAGTGCCG 
     3961 ATTATTTTAT GCAAGAAGCT AAACGAATGA AGCATAAAGC AGATGCAATG GTGGAAAAGT 
     4021 TTGGAAAGGC TTTGAACTAT GCTGAAGCAG CATTGTCGTT TATCGAGTGT GGAAATGCAA 
     4081 TGGAACAAGG CCCCATGGAA TCCAAATCTC CTTATACGAT GTATTCAGAA ACAGTAGAGC 
     4141 TCATCAGGTA TGCTATGAGA CTAAAAACCC ACTCAGGCCC CAATGCCACA CCAGAAGACA 
     4201 AACAACTGGC TGCATTATGT TACCGATGCC TGGCCCTCCT GTACTGGCGG ATGTTTCGAC 
     4261 TCAAAAGGGA CCACGCTGTA AAGTATTCAA AAGCACTAAT CGACTATTTC AAGAACTCAT 
     4321 CTAAAGCCGC CCAAGCCCCA TCTCCGTGGG GGGCCAGTGG AAAGAGCACT GGAACCCCAT 
     4381 CCCCCATGTC TCCCAACCCC TCTCCCGCCA GCTCCGTGGG GTCTCAGGGC AGCCTCTCCA 
     4441 ACGCCAGCGC CCTGTCCCCG TCGACCATCG TCAGCATCCC ACAGCGCATC CACCAGATGG 
     4501 CGGCCAACCA CGTCAGCATC ACCAACAGCA TCCTGCACAG CTACGACTAC TGGGAGATGG 
     4561 CCGACAACCT GGCCAAGGAA AACCGAGAAT TCTTCAACGA CCTGGATCTG CTCATGGGGC 
     4621 CGGTCACCCT GCACAGCAGC ATGGAGCACC TGGTCCAGTA CTCCCAACAG GGCCTGCACT 
     4681 GGCTGCGGAA CAGCGCCCAC CTGTCAACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4741 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4801 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4861 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4921 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4981 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     5041 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     5101 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     5161 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5221 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5281 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5341 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5401 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5461 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5521 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5581 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5641 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5701 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5761 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5821 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5881 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5941 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     6001 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     6061 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     6121 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6181 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6241 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6301 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6361 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6421 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6481 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6541 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6601 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6661 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6721 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6781 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6841 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6901 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6961 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     7021 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     7081 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     7141 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7201 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7261 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7321 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7381 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7441 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7501 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7561 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7621 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7681 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7741 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7801 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7861 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7921 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7981 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8041 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     8101 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     8161 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8221 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8281 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8341 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8401 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8461 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8521 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//