LOCUS       RC221611L1                8970 bp    DNA     circular  SYN 
DEFINITION  PROM1 (Myc-DDK-tagged)-Human prominin 1 (PROM1), transcript variant 1
ACCESSION   NM_006017
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7261..7441 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8169..8828 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7600..8168 
                     /label="ColE1"  
     misc_feature    6136..6156 
                     /label="LR50 primer"  
     misc_feature    5933..6004 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6145..6736 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5915..5921 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5915 
                     /label="PROM1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCCTCGTA CTCGGCTCCC TGTTGCTGCT 
     3361 GGGGCTGTGC GGGAACTCCT TTTCAGGAGG GCAGCCTTCA TCCACAGATG CTCCTAAGGC 
     3421 TTGGAATTAT GAATTGCCTG CAACAAATTA TGAGACCCAA GACTCCCATA AAGCTGGACC 
     3481 CATTGGCATT CTCTTTGAAC TAGTGCATAT CTTTCTCTAT GTGGTACAGC CGCGTGATTT 
     3541 CCCAGAAGAT ACTTTGAGAA AATTCTTACA GAAGGCATAT GAATCCAAAA TTGATTATGA 
     3601 CAAGCCAGAA ACTGTAATCT TAGGTCTAAA GATTGTCTAC TATGAAGCAG GGATTATTCT 
     3661 ATGCTGTGTC CTGGGGCTGC TGTTTATTAT TCTGATGCCT CTGGTGGGGT ATTTCTTTTG 
     3721 TATGTGTCGT TGCTGTAACA AATGTGGTGG AGAAATGCAC CAGCGACAGA AGGAAAATGG 
     3781 GCCCTTCCTG AGGAAATGCT TTGCAATCTC CCTGTTGGTG ATTTGTATAA TAATAAGCAT 
     3841 TGGCATCTTC TATGGTTTTG TGGCAAATCA CCAGGTAAGA ACCCGGATCA AAAGGAGTCG 
     3901 GAAACTGGCA GATAGCAATT TCAAGGACTT GCGAACTCTC TTGAATGAAA CTCCAGAGCA 
     3961 AATCAAATAT ATATTGGCCC AGTACAACAC TACCAAGGAC AAGGCGTTCA CAGATCTGAA 
     4021 CAGTATCAAT TCAGTGCTAG GAGGCGGAAT TCTTGACCGA CTGAGACCCA ACATCATCCC 
     4081 TGTTCTTGAT GAGATTAAGT CCATGGCAAC AGCGATCAAG GAGACCAAAG AGGCGTTGGA 
     4141 GAACATGAAC AGCACCTTGA AGAGCTTGCA CCAACAAAGT ACACAGCTTA GCAGCAGTCT 
     4201 GACCAGCGTG AAAACTAGCC TGCGGTCATC TCTCAATGAC CCTCTGTGCT TGGTGCATCC 
     4261 ATCAAGTGAA ACCTGCAACA GCATCAGATT GTCTCTAAGC CAGCTGAATA GCAACCCTGA 
     4321 ACTGAGGCAG CTTCCACCCG TGGATGCAGA ACTTGACAAC GTTAATAACG TTCTTAGGAC 
     4381 AGATTTGGAT GGCCTGGTCC AACAGGGCTA TCAATCCCTT AATGATATAC CTGACAGAGT 
     4441 ACAACGCCAA ACCACGACTG TCGTAGCAGG TATCAAAAGG GTCTTGAATT CCATTGGTTC 
     4501 AGATATCGAC AATGTAACTC AGCGTCTTCC TATTCAGGAT ATACTCTCAG CATTCTCTGT 
     4561 TTATGTTAAT AACACTGAAA GTTACATCCA CAGAAATTTA CCTACATTGG AAGAGTATGA 
     4621 TTCATACTGG TGGCTGGGTG GCCTGGTCAT CTGCTCTCTG CTGACCCTCA TCGTGATTTT 
     4681 TTACTACCTG GGCTTACTGT GTGGCGTGTG CGGCTATGAC AGGCATGCCA CCCCGACCAC 
     4741 CCGAGGCTGT GTCTCCAACA CCGGAGGCGT CTTCCTCATG GTTGGAGTTG GATTAAGTTT 
     4801 CCTCTTTTGC TGGATATTGA TGATCATTGT GGTTCTTACC TTTGTCTTTG GTGCAAATGT 
     4861 GGAAAAACTG ATCTGTGAAC CTTACACGAG CAAGGAATTA TTCCGGGTTT TGGATACACC 
     4921 CTACTTACTA AATGAAGACT GGGAATACTA TCTCTCTGGG AAGCTATTTA ATAAATCAAA 
     4981 AATGAAGCTC ACTTTTGAAC AAGTTTACAG TGACTGCAAA AAAAATAGAG GCACTTACGG 
     5041 CACTCTTCAC CTGCAGAACA GCTTCAATAT CAGTGAACAT CTCAACATTA ATGAGCATAC 
     5101 TGGAAGCATA AGCAGTGAAT TGGAAAGTCT GAAGGTAAAT CTTAATATCT TTCTGTTGGG 
     5161 TGCAGCAGGA AGAAAAAACC TTCAGGATTT TGCTGCTTGT GGAATAGACA GAATGAATTA 
     5221 TGACAGCTAC TTGGCTCAGA CTGGTAAATC CCCCGCAGGA GTGAATCTTT TATCATTTGC 
     5281 ATATGATCTA GAAGCAAAAG CAAACAGTTT GCCCCCAGGA AATTTGAGGA ACTCCCTGAA 
     5341 AAGAGATGCA CAAACTATTA AAACAATTCA CCAGCAACGA GTCCTTCCTA TAGAACAATC 
     5401 ACTGAGCACT CTATACCAAA GCGTCAAGAT ACTTCAACGC ACAGGGAATG GATTGTTGGA 
     5461 GAGAGTAACT AGGATTCTAG CTTCTCTGGA TTTTGCTCAG AACTTCATCA CAAACAATAC 
     5521 TTCCTCTGTT ATTATTGAGG AAACTAAGAA GTATGGGAGA ACAATAATAG GATATTTTGA 
     5581 ACATTATCTG CAGTGGATCG AGTTCTCTAT CAGTGAGAAA GTGGCATCGT GCAAACCTGT 
     5641 GGCCACCGCT CTAGATACTG CTGTTGATGT CTTTCTGTGT AGCTACATTA TCGACCCCTT 
     5701 GAATTTGTTT TGGTTTGGCA TAGGAAAAGC TACTGTATTT TTACTTCCGG CTCTAATTTT 
     5761 TGCGGTAAAA CTGGCTAAGT ACTATCGTCG AATGGATTCG GAGGACGTGT ACGATGATGT 
     5821 TGAAACTATA CCCATGAAAA ATATGGAAAA TGGTAATAAT GGTTATCATA AAGATCATGT 
     5881 ATATGGTATT CACAATCCTG TTATGACAAG CCCATCACAA CATACGCGTA CGCGGCCGCT 
     5941 CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA 
     6001 TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCTGTAC AAGTAGGATT CGTCGAGGGA 
     6061 CCTAATAACT TCGTATAGCA TACATTATAC GAAGTTATAC ATGTTTAAGG GTTCCGGTTC 
     6121 CACTAGGTAC AATTCGATAT CAAGCTTATC GATAATCAAC CTCTGGATTA CAAAATTTGT 
     6181 GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT 
     6241 TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT 
     6301 AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG 
     6361 GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG 
     6421 CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC 
     6481 TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG 
     6541 TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC 
     6601 GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC 
     6661 CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC 
     6721 TCCCTTTGGG CCGCCTCCCC GCATCGATAC CGTCGACCTC GATCGAGACC TAGAAAAACA 
     6781 TGGAGCAATC ACAAGTAGCA ATACAGCAGC TACCAATGCT GATTGTGCCT GGCTAGAAGC 
     6841 ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC 
     6901 TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT 
     6961 AATTCACTCC CAACGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA 
     7021 CTTCCCTGAT TGGCAGAACT ACACACCAGG GCCAGGGATC AGATATCCAC TGACCTTTGG 
     7081 ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA 
     7141 GAACACCCGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT 
     7201 ATTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC ATGGCCCGAG AGCTGCATCC 
     7261 GGACTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT 
     7321 AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC 
     7381 CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA 
     7441 AATCTCTAGC AGCATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     7501 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     7561 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     7621 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     7681 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     7741 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     7801 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     7861 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     7921 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     7981 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     8041 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     8101 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     8161 AGGGATTTTG GTCATGATTA CGCCCCGCCC TGCCACTCAT CGCAGTACTG TTGTAATTCA 
     8221 TTAAGCATTC TGCCGACATG GAAGCCATCA CAAACGGCAT GATGAACCTG AATCGCCAGC 
     8281 GGCATCAGCA CCTTGTCGCC TTGCGTATAA TATTTGCCCA TGGTGAAAAC GGGGGCGAAG 
     8341 AAGTTGTCCA TATTGGCCAC GTTTAAATCA AAACTGGTGA AACTCACCCA GGGATTGGCT 
     8401 GAGACGAAAA ACATATTCTC AATAAACCCT TTAGGGAAAT AGGCCAGGTT TTCACCGTAA 
     8461 CACGCCACAT CTTGCGAATA TATGTGTAGA AACTGCCGGA AATCGTCGTG GTATTCACTC 
     8521 CAGAGCGATG AAAACGTTTC AGTTTGCTCA TGGAAAACGG TGTAACAAGG GTGAACACTA 
     8581 TCCCATATCA CCAGCTCACC GTCTTTCATT GCCATACGGA ACTCCGGATG AGCATTCATC 
     8641 AGGCGGGCAA GAATGTGAAT AAAGGCCGGA TAAAACTTGT GCTTATTTTT CTTTACGGTC 
     8701 TTTAAAAAGG CCGTAATATC CAGCTGAACG GTCTGGTTAT AGGTACATTG AGCAACTGAC 
     8761 TGAAATGCCT CAAAATGTTC TTTACGATGC CATTGGGATA TATCAACGGT GGTATATCCA 
     8821 GTGATTTTTT TCTCCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     8881 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTCCCG 
     8941 CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//