LOCUS       RC221611                7476 bp    DNA     circular  SYN 
DEFINITION  PROM1 (Myc-DDK-tagged)-Human prominin 1 (PROM1), transcript variant 1
ACCESSION   NM_006017
VERSION     NM_006017.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3633..3724 
                     /label="Myc-DDK"  
     misc_feature    3754..4339 
                     /label="PolyA signal"  
     misc_feature    3811..3830 
                     /label="Restriction Enzyme"  
     misc_feature    4615..5287 
                     /label="ColE1"  
     misc_feature    5717..6511 
                     /label="Kan/Neo"  
     misc_feature    6546..6848 
                     /label="SV40 ori"  
     misc_feature    6934..7411 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3615..3621 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3615 
                     /label="PROM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCTCGTA CTCGGCTCCC TGTTGCTGCT GGGGCTGTGC GGGAACTCCT 
     1081 TTTCAGGAGG GCAGCCTTCA TCCACAGATG CTCCTAAGGC TTGGAATTAT GAATTGCCTG 
     1141 CAACAAATTA TGAGACCCAA GACTCCCATA AAGCTGGACC CATTGGCATT CTCTTTGAAC 
     1201 TAGTGCATAT CTTTCTCTAT GTGGTACAGC CGCGTGATTT CCCAGAAGAT ACTTTGAGAA 
     1261 AATTCTTACA GAAGGCATAT GAATCCAAAA TTGATTATGA CAAGCCAGAA ACTGTAATCT 
     1321 TAGGTCTAAA GATTGTCTAC TATGAAGCAG GGATTATTCT ATGCTGTGTC CTGGGGCTGC 
     1381 TGTTTATTAT TCTGATGCCT CTGGTGGGGT ATTTCTTTTG TATGTGTCGT TGCTGTAACA 
     1441 AATGTGGTGG AGAAATGCAC CAGCGACAGA AGGAAAATGG GCCCTTCCTG AGGAAATGCT 
     1501 TTGCAATCTC CCTGTTGGTG ATTTGTATAA TAATAAGCAT TGGCATCTTC TATGGTTTTG 
     1561 TGGCAAATCA CCAGGTAAGA ACCCGGATCA AAAGGAGTCG GAAACTGGCA GATAGCAATT 
     1621 TCAAGGACTT GCGAACTCTC TTGAATGAAA CTCCAGAGCA AATCAAATAT ATATTGGCCC 
     1681 AGTACAACAC TACCAAGGAC AAGGCGTTCA CAGATCTGAA CAGTATCAAT TCAGTGCTAG 
     1741 GAGGCGGAAT TCTTGACCGA CTGAGACCCA ACATCATCCC TGTTCTTGAT GAGATTAAGT 
     1801 CCATGGCAAC AGCGATCAAG GAGACCAAAG AGGCGTTGGA GAACATGAAC AGCACCTTGA 
     1861 AGAGCTTGCA CCAACAAAGT ACACAGCTTA GCAGCAGTCT GACCAGCGTG AAAACTAGCC 
     1921 TGCGGTCATC TCTCAATGAC CCTCTGTGCT TGGTGCATCC ATCAAGTGAA ACCTGCAACA 
     1981 GCATCAGATT GTCTCTAAGC CAGCTGAATA GCAACCCTGA ACTGAGGCAG CTTCCACCCG 
     2041 TGGATGCAGA ACTTGACAAC GTTAATAACG TTCTTAGGAC AGATTTGGAT GGCCTGGTCC 
     2101 AACAGGGCTA TCAATCCCTT AATGATATAC CTGACAGAGT ACAACGCCAA ACCACGACTG 
     2161 TCGTAGCAGG TATCAAAAGG GTCTTGAATT CCATTGGTTC AGATATCGAC AATGTAACTC 
     2221 AGCGTCTTCC TATTCAGGAT ATACTCTCAG CATTCTCTGT TTATGTTAAT AACACTGAAA 
     2281 GTTACATCCA CAGAAATTTA CCTACATTGG AAGAGTATGA TTCATACTGG TGGCTGGGTG 
     2341 GCCTGGTCAT CTGCTCTCTG CTGACCCTCA TCGTGATTTT TTACTACCTG GGCTTACTGT 
     2401 GTGGCGTGTG CGGCTATGAC AGGCATGCCA CCCCGACCAC CCGAGGCTGT GTCTCCAACA 
     2461 CCGGAGGCGT CTTCCTCATG GTTGGAGTTG GATTAAGTTT CCTCTTTTGC TGGATATTGA 
     2521 TGATCATTGT GGTTCTTACC TTTGTCTTTG GTGCAAATGT GGAAAAACTG ATCTGTGAAC 
     2581 CTTACACGAG CAAGGAATTA TTCCGGGTTT TGGATACACC CTACTTACTA AATGAAGACT 
     2641 GGGAATACTA TCTCTCTGGG AAGCTATTTA ATAAATCAAA AATGAAGCTC ACTTTTGAAC 
     2701 AAGTTTACAG TGACTGCAAA AAAAATAGAG GCACTTACGG CACTCTTCAC CTGCAGAACA 
     2761 GCTTCAATAT CAGTGAACAT CTCAACATTA ATGAGCATAC TGGAAGCATA AGCAGTGAAT 
     2821 TGGAAAGTCT GAAGGTAAAT CTTAATATCT TTCTGTTGGG TGCAGCAGGA AGAAAAAACC 
     2881 TTCAGGATTT TGCTGCTTGT GGAATAGACA GAATGAATTA TGACAGCTAC TTGGCTCAGA 
     2941 CTGGTAAATC CCCCGCAGGA GTGAATCTTT TATCATTTGC ATATGATCTA GAAGCAAAAG 
     3001 CAAACAGTTT GCCCCCAGGA AATTTGAGGA ACTCCCTGAA AAGAGATGCA CAAACTATTA 
     3061 AAACAATTCA CCAGCAACGA GTCCTTCCTA TAGAACAATC ACTGAGCACT CTATACCAAA 
     3121 GCGTCAAGAT ACTTCAACGC ACAGGGAATG GATTGTTGGA GAGAGTAACT AGGATTCTAG 
     3181 CTTCTCTGGA TTTTGCTCAG AACTTCATCA CAAACAATAC TTCCTCTGTT ATTATTGAGG 
     3241 AAACTAAGAA GTATGGGAGA ACAATAATAG GATATTTTGA ACATTATCTG CAGTGGATCG 
     3301 AGTTCTCTAT CAGTGAGAAA GTGGCATCGT GCAAACCTGT GGCCACCGCT CTAGATACTG 
     3361 CTGTTGATGT CTTTCTGTGT AGCTACATTA TCGACCCCTT GAATTTGTTT TGGTTTGGCA 
     3421 TAGGAAAAGC TACTGTATTT TTACTTCCGG CTCTAATTTT TGCGGTAAAA CTGGCTAAGT 
     3481 ACTATCGTCG AATGGATTCG GAGGACGTGT ACGATGATGT TGAAACTATA CCCATGAAAA 
     3541 ATATGGAAAA TGGTAATAAT GGTTATCATA AAGATCATGT ATATGGTATT CACAATCCTG 
     3601 TTATGACAAG CCCATCACAA CATACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3661 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3721 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3781 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3841 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3901 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3961 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4021 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4081 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4141 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4201 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4261 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4321 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4381 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4441 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4501 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4561 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4621 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4681 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4741 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4801 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4861 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4921 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4981 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5041 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5101 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5161 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5221 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5281 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5341 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5401 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5461 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5521 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5581 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5641 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5701 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5761 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5821 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5881 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5941 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6001 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6061 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6121 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6181 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6241 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6301 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6361 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6421 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6481 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6541 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6601 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6661 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6721 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6781 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6841 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6901 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6961 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7021 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7081 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7141 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7201 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7261 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7321 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7381 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7441 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//