LOCUS       RC221586                8427 bp    DNA     circular  SYN 
DEFINITION  MYT1L (Myc-DDK-tagged)-Human myelin transcription factor 1-like (MYT1L)
ACCESSION   NM_015025
VERSION     NM_015025.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4582..4673 
                     /label="Myc-DDK"  
     misc_feature    4703..5288 
                     /label="PolyA signal"  
     misc_feature    4760..4779 
                     /label="Restriction Enzyme"  
     misc_feature    5564..6236 
                     /label="ColE1"  
     misc_feature    6666..7460 
                     /label="Kan/Neo"  
     misc_feature    7495..7797 
                     /label="SV40 ori"  
     misc_feature    7883..8360 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4572..4580 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4572 
                     /label="MYT1L"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGTGGAC ACCGAGGAGA AGCGGCATCG CACGCGGTCC AAAGGGGTTC 
     1081 GAGTTCCCGT GGAACCAGCC ATACAAGAGC TGTTCAGCTG TCCCACCCCT GGCTGTGACG 
     1141 GCAGTGGTCA TGTCAGTGGC AAATATGCAA GACACAGAAG TGTATATGGT TGTCCCTTGG 
     1201 CGAAAAAAAG AAAAACACAA GATAAACAGC CCCAGGAACC TGCTCCTAAA CGAAAGCCAT 
     1261 TTGCCGTGAA AGCAGACAGC TCCTCAGTGG ATGAGTGTGA CGACAGTGAT GGGACTGAGG 
     1321 ACATGGATGA GAAGGAGGAG GATGAGGGGG AGGAGTACTC CGAGGACAAT GACGAGCCAG 
     1381 GGGATGAGGA CGAGGAGGAC GAGGAGGGGG ACCGGGAGGA GGAGGAGGAG ATCGAGGAGG 
     1441 AGGATGAGGA CGATGACGAG GATGGAGAAG ATGTGGAGGA TGAAGAAGAG GAAGAGGAGG 
     1501 AGGAGGAGGA GGAGGAAGAG GAAGAAGAAA ACGAAGACCA TCAAATGAAT TGTCACAATA 
     1561 CTCGAATAAT GCAAGACACA GAAAAGGATG ATAACAATAA TGACGAATAT GACAATTACG 
     1621 ATGAACTGGT GGCCAAGTCA TTGTTAAACC TCGGCAAAAT CGCTGAGGAT GCAGCCTACC 
     1681 GGGCCAGGAC TGAGTCAGAA ATGAACAGCA ATACCTCCAA TAGTCTGGAA GACGATAGTG 
     1741 ACAAAAACGA AAACCTGGGT CGGAAAAGTG AGTTGAGTTT AGACTTAGAC AGTGATGTTG 
     1801 TTAGAGAAAC AGTGGACTCC CTTAAACTAT TAGCCCAAGG ACACGGTGTT GTGCTCTCAG 
     1861 AAAACATGAA TGACAGAAAT TATGCAGACA GCATGTCGCA GCAAGACAGT AGAAATATGA 
     1921 ATTACGTCAT GTTGGGGAAG CCCATGAACA ATGGACTCAT GGAAAAGATG GTGGAGGAGA 
     1981 GCGATGAGGA GGTGTGTCTG AGCAGTCTGG AGTGTTTGAG GAATCAGTGC TTCGACCTGG 
     2041 CCAGGAAGCT CAGTGAGACC AACCCGCAGG AGAGGAATCC GCAGCAGAAC ATGAACATCC 
     2101 GTCAGCATGT CCGGCCAGAA GAGGACTTCC CAGGAAGGAC GCCGGACAGA AACTACTCGG 
     2161 ACATGCTGAA CCTCATGCGG CTGGAGGAGC AGTTGAGCCC CCGGTCGAGA GTGTTTGCCA 
     2221 GCTGTGCGAA GGAGGATGGG TGTCATGAGC GGGACGACGA TACCACCTCT GTGAACTCGG 
     2281 ACAGGTCTGA AGAGGTGTTC GACATGACCA AGGGGAACCT GACCCTGCTG GAGAAAGCCA 
     2341 TCGCTTTGGA AACGGAAAGA GCAAAGGCCA TGAGGGAGAA GATGGCCATG GAAGCTGGGA 
     2401 GGAGGGACAA TATGAGGTCA TATGAGGACC AGTCTCCGAG ACAACTTCCC GGGGAGGACA 
     2461 GAAAGCCTAA ATCCAGTGAC AGCCATGTCA AAAAGCCATA CTATGATCCC TCAAGAACAG 
     2521 AAAAGAAAGA GAGCAAGTGT CCAACCCCCG GGTGTGATGG AACCGGCCAC GTAACTGGGC 
     2581 TGTACCCACA TCACCGCAGC CTGTCCGGAT GCCCGCACAA AGATAGGGTC CCTCCAGAAA 
     2641 TCCTTGCCAT GCATGAAAGT GTCCTCAAGT GCCCCACTCC GGGCTGCACG GGGCGCGGGC 
     2701 ATGTCAACAG CAACAGGAAC TCCCACCGAA GCCTCTCCGG ATGCCCGATC GCTGCAGCAG 
     2761 AGAAACTGGC CAAGGCACAG GAAAAGCACC AGAGCTGCGA CGTGTCCAAG TCCAGCCAGG 
     2821 CCTCGGACCG CGTGCTCAGG CCAATGTGCT TTGTGAAGCA GCTGGAGATT CCTCAGTATG 
     2881 GCTACAGAAA CAATGTCCCC ACAACTACGC CGCGTTCCAA CCTGGCCAAG GAGCTCGAGA 
     2941 AATATTCCAA GACCTCGTTT GAATACAACA GTTACGACAA CCATACTTAT GGCAAGCGAG 
     3001 CCATAGCTCC CAAGGTGCAA ACCAGGGATA TATCCCCCAA AGGATATGAT GATGCGAAGC 
     3061 GGTACTGCAA GGACCCCAGC CCCAGCAGCA GCAGCACCAG CAGCTACGCG CCCAGCAGCA 
     3121 GCAGCAACCT GAGCTGCGGC GGGGGCAGCA GCGCCAGCAG CACGTGCAGC AAGAGCAGCT 
     3181 TCGACTACAC GCACGACATG GAGGCGGCCC ACATGGCGGC CACCGCCATC CTCAACCTGT 
     3241 CCACGCGCTG CCGCGAGATG CCGCAGAACC TGAGCACCAA GCCGCAGGAC CTGTGCGCCA 
     3301 CGCGGAACCC TGACATGGAG GTGGATGAGA ACGGGACCCT GGACCTCAGC ATGAACAAGC 
     3361 AGAGGCCGCG GGACAGCTGC TGCCCCATCC TGACCCCTCT GGAGCCCATG TCCCCCCAGC 
     3421 AGCAGGCAGT GATGAACAAC CGGTGTTTCC AGCTGGGCGA GGGCGACTGC TGGGACTTGC 
     3481 CCGTAGACTA CACCAAAATG AAACCCCGGA GGATAGACGA GGACGAGTCC AAAGACATTA 
     3541 CTCCAGAAGA CTTGGACCCA TTCCAGGAGG CTCTAGAAGA AAGACGGTAT CCCGGGGAGG 
     3601 TGACCATCCC AAGTCCCAAA CCCAAGTACC CTCAGTGCAA GGAGAGCAAA AAGGACTTAA 
     3661 TAACTCTGTC TGGCTGCCCC CTGGCGGACA AAAGCATTCG AAGTATGCTG GCCACCAGCT 
     3721 CCCAAGAACT CAAGTGCCCC ACGCCTGGCT GTGATGGTTC TGGACATATC ACCGGCAATT 
     3781 ATGCTTCTCA TCGGAGCCTT TCAGGTTGCC CAAGAGCAAA GAAAAGTGGT ATCAGGATAG 
     3841 CACAGAGCAA AGAAGATAAA GAAGATCAAG AACCCATCAG GTGTCCGGTC CCCGGGTGCG 
     3901 ACGGCCAGGG CCACATCACT GGGAAGTACG CGTCCCATCG CAGCGCCTCC GGGTGCCCCT 
     3961 TGGCGGCCAA GAGGCAGAAA GACGGGTACC TGAATGGCTC CCAGTTCTCC TGGAAGTCGG 
     4021 TCAAGACGGA AGGCATGTCC TGCCCCACGC CAGGATGCGA CGGCTCAGGC CACGTCAGCG 
     4081 GCAGCTTCCT CACACACCGC AGCTTGTCAG GATGCCCGAG AGCCACGTCA GCGATGAAGA 
     4141 AGGCAAAGCT TTCTGGAGAG CAGATGCTGA CCATCAAACA GCGGGCCAGC AACGGTATAG 
     4201 AAAATGATGA AGAAATCAAA CAGTTAGATG AAGAAATCAA GGAGCTAAAT GAATCCAATT 
     4261 CCCAGATGGA AGCCGATATG ATTAAACTCA GAACTCAGAT TACCACGATG GAGAGCAACC 
     4321 TGAAGACCAT CGAAGAGGAG AACAAAGTGA TTGAGCAGCA GAACGAGTCT CTCCTCCACG 
     4381 AGCTGGCGAA CCTGAGCCAG TCTCTGATCC ACAGCCTGGC TAACATCCAG CTGCCGCACA 
     4441 TGGATCCAAT CAATGAACAA AATTTTGATG CTTACGTGAC TACTTTGACG GAAATGTATA 
     4501 CAAATCAAGA TCGTTATCAG AGTCCAGAAA ATAAAGCCCT ACTGGAAAAT ATAAAGCAGG 
     4561 CTGTGAGAGG AATTCAGGTC ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4621 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4681 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4741 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4801 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4861 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4921 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4981 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5041 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5101 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5161 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5221 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5281 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5341 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5401 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5461 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5521 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5581 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5641 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5701 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5761 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5821 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5881 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5941 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6001 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6061 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6121 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6181 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6241 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6301 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6361 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6421 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6481 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6541 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6601 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6661 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6721 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6781 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6841 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6901 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6961 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7021 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7081 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7141 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7201 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7261 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7321 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7381 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7441 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7501 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7561 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7621 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7681 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7741 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7801 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7861 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7921 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7981 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8041 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8101 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8161 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8221 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8281 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8341 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8401 ATTTAACAAA AATTTAACGC GAATTTT
//