LOCUS       RC221579                7167 bp    DNA     circular  SYN 
DEFINITION  KIAA1751 (Myc-DDK-tagged)-Human KIAA1751 (KIAA1751)
ACCESSION   NM_001080484
VERSION     NM_001080484.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3324..3415 
                     /label="Myc-DDK"  
     misc_feature    3445..4030 
                     /label="PolyA signal"  
     misc_feature    3502..3521 
                     /label="Restriction Enzyme"  
     misc_feature    4306..4978 
                     /label="ColE1"  
     misc_feature    5408..6202 
                     /label="Kan/Neo"  
     misc_feature    6237..6539 
                     /label="SV40 ori"  
     misc_feature    6625..7102 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3306..3312 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3306 
                     /label="CFAP74"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGATGAC GGCAGCCTGC TCCCTGAGGA CGAGCTTTTG GCCGATGCCC 
     1081 TTCTTTTGGA AGATGAAAGA GATGAATTAG AGGATCCGGA GTTTGACATC AAATGTCTTC 
     1141 TACAGGAAGC TGAGGATGAC GTGGACCCGG GACACAGCAG CTCAGTGAAA GAACTAGATA 
     1201 CTGATGCTGA TAAATTGAAG AAGAAAACAG CTGAGGACAG AACGCAGGCA TTTCACCTGC 
     1261 GGCAGAACCT GAGCGCCCTG GATAAGATGC ATGAGGAGCA AGAGCTTTTC ACTGAGAAGA 
     1321 TGAGAGGGGA GCTGCGCGCC TGTCGGCAGA GGCGGGACCT GATCGACAAG CAGCAGGAGG 
     1381 CTGTGGCAGC CGAGATCGCC ACAGAGGAAG AGGCGGGCAA CATGGCCGCT GTGGGCCGCC 
     1441 TCCAGGCCGT GTCCAGACGC CTGTTTGCAG AGCTGGAGAA CGAGAGAGAC CTGCAGTCGA 
     1501 GGACTGAGGC CGTGCTGAAG GAGAGCGAGA ACACCATGTG GCACATCGAG ATCCAGGAGG 
     1561 GGCGACTCGA GGCCTTCCGG ACAGCTGACC GTGAGGAGGT GGAGGCCACG GGGCGGCGGC 
     1621 TCCAGGTGCG CGCAGCCGAG CAGCTCTGCA GAGAGCAGGA GGCCCTGGGG AAGGTGGAGC 
     1681 GGAACCGACT GCTGAGGATC AGGAAGTCCC TGAACACCCA GAAGGAGCTC GGGCTCAGGC 
     1741 ACCAGAAGCT GCTGGAGGAC GCCCGGAAGA ACCACAAGGT TGCCGTGCGG TTCCTGAAGG 
     1801 CCTCCCTGGG AAGAATCCGA GAGCAAGAGA AGAAGGAGGA GATGGAGTGC CACGAGTACA 
     1861 TGAGGCGACG CATGGATGCG GTGGTGGCGC TGAAGGGCAG CATCTCCGCG AACCGGGACA 
     1921 CACTGCGGAA GTTCCAAGCA TGGGACCGTG CCAAGGCAGA GCTGGCGGAG CAGAGGGTCC 
     1981 AGGCTGAGAA GAAGGCGATT CTGGCCCAGG GCAGGGATGC ATTCAGGCAC CTTGTCCACC 
     2041 AGCGCCGGCG CCAGGAGCTG GAGGCCCAGA AGAGGGCCTT TGAGGAGGAG CAGAAGCTCA 
     2101 GAAAGCAGGA GATCATCAGT CGGATTCTGA AAGAGGAGGC TGAGGAGGAA AAGAGGAAGA 
     2161 AACAGCATCC CCCCACCAGT GCCAGGCACC GGCTGACCCT GAGGGACAAG ACCTGGAACT 
     2221 ACATTTCTGA CTTTTGCAAG AAGACCACAG TCCCAACCAA CACGTACACA CTGGACTACG 
     2281 AGGCTGCTGC AGGCCCCGGG CCCTCTCGGT TGCTGGAAGT CGTTTCCAGT GAGCTTATCC 
     2341 AGGGGGACCC CGGGGCCAGC TCAGAGGAGG AAACGTTAGC TGAGCCCGAG ATCTCTGGGC 
     2401 TTTGGAATGA AGACTACAAG CCATACCAGG TGCCCAAGGA GGACGTGGAC CGAAAGCCCG 
     2461 TGGGCGGGAC AAAGATGGAC AAGGACATCC TGGAGCGCAC GGTGGAGCGG CTGCGGAGCA 
     2521 GGGTGGTCCA CAAGCAGGTG GTGTGGGGGC GTGAGTTCCA AGGACGCCCC TTCAACAGCA 
     2581 AACCGGAGCT CCTCCACTTC CAGGACTTTG ATATTGGCAA AGTGTACAAG AAAAAGATCA 
     2641 CGTTGGTAAA CACCACCTAC ACGATCAACT ACTGCAAGCT GGTGGGCGTG GAGGAGCACC 
     2701 TCCGGGACTT CATCCACGTT GACTTTGACC CCCCTGGCCC CCTGTCAGCC GGAATGTCCT 
     2761 GTGAAGTGCT TGTCACCTTC AAGCCGATGA TAAACAAGGA TCTAGAAGGA AATATCTCAT 
     2821 TTTTGGCTCA GACGGGCGAG TTTTCAGTTC CACTGAAATG TTCAACAAAG AAATGTTCGC 
     2881 TGTCCCTCGA CAAGGAGCTC ATTGACTTCG GCAGCTACGT GGTAGGAGAG ACCACGTCTC 
     2941 GGACCATCAC GCTGACCAAC GTTGGGGGCT TGGGCACGAC TTTCAAGTTC CTGCCAGCTT 
     3001 CAGAGCCCTG TGAGATGGAC GACTCCCAGT CTGCCCTGAA ATTAAGCAGT CTCCTGACCT 
     3061 ACGAAGATAA AAGCTTGTAT GACAAAGCCG CCACCAGCTT CTCTGAGCAG CAGCTAGAGG 
     3121 GCACGGAGTC CTCCCAGGCG GACATGCAGA GCCGGAAGGA GCTGGAGAAG CTGGACAAGG 
     3181 AGCAGGAGGA GGAGCAGCCC GCAGGTGAGC CAGGCTGCCA GGCCAAGCCA GGGTGGGCGG 
     3241 GCGGTGAGGG CCTCAGGGCA GCATTCCAGG GGGCTGAGGG GCCCAGGGGA GGGCTGGAAA 
     3301 GTAGAGAATT TTCCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3361 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3421 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3481 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3541 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3601 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3661 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3721 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3781 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3841 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3901 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3961 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4021 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4081 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4141 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4201 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4261 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4321 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4381 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4441 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4501 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4561 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4621 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4681 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4741 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4801 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4861 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4921 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4981 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5041 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5101 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5161 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5221 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5281 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5341 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5401 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5461 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5521 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5581 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5641 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5701 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5761 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5821 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5881 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5941 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6001 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6061 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6121 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6181 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6241 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6301 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6361 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6421 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6481 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6541 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6601 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6661 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6721 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6781 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6841 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6901 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6961 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7021 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7081 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7141 ATTTAACAAA AATTTAACGC GAATTTT
//