LOCUS       RC221556                7266 bp    DNA     circular  SYN 
DEFINITION  MARK1 (Myc-DDK-tagged)-Human MAP/microtubule affinity-regulating kinase 1 (MARK1)
ACCESSION   NM_018650
VERSION     NM_018650.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3423..3514 
                     /label="Myc-DDK"  
     misc_feature    3544..4129 
                     /label="PolyA signal"  
     misc_feature    3601..3620 
                     /label="Restriction Enzyme"  
     misc_feature    4405..5077 
                     /label="ColE1"  
     misc_feature    5507..6301 
                     /label="Kan/Neo"  
     misc_feature    6336..6638 
                     /label="SV40 ori"  
     misc_feature    6724..7201 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3405..3411 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3405 
                     /label="MARK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGCCCGG ACGCCATTGC CGACGGTGAA CGAGCGGGAC ACGGAAAATC 
     1081 ATACATCTGT GGATGGATAT ACTGAACCAC ACATCCAGCC TACCAAGTCG AGTAGCAGAC 
     1141 AGAACATCCC CCGGTGTAGA AACTCCATTA CGTCAGCAAC AGATGAACAG CCTCACATTG 
     1201 GAAATTACCG TTTACAAAAA ACAATAGGGA AGGGAAATTT TGCCAAAGTC AAATTGGCAA 
     1261 GACACGTTCT AACTGGTAGA GAGGTTGCTG TGAAAATAAT AGACAAAACT CAGCTAAATC 
     1321 CTACCAGTCT ACAAAAGTTA TTTCGAGAAG TACGAATAAT GAAGATACTG AATCATCCTA 
     1381 ATATAGTAAA ATTGTTTGAA GTTATTGAAA CAGAGAAGAC TCTCTATTTA GTCATGGAAT 
     1441 ACGCGAGTGG GGGTGAAGTA TTTGATTACT TAGTTGCCCA TGGAAGAATG AAAGAGAAAG 
     1501 AGGCCCGTGC AAAATTTAGG CAGATTGTAT CTGCTGTACA GTATTGTCAT CAAAAGTACA 
     1561 TTGTTCACCG TGATCTTAAG GCTGAAAACC TTCTCCTTGA TGGTGATATG AATATTAAAA 
     1621 TTGCTGACTT TGGTTTTAGT AATGAATTTA CAGTTGGGAA CAAATTGGAC ACATTTTGTG 
     1681 GAAGCCCACC CTATGCTGCT CCCGAGCTTT TCCAAGGAAA GAAGTATGAT GGGCCTGAAG 
     1741 TGGATGTGTG GAGTCTGGGC GTCATTCTCT ATACATTAGT CAGTGGCTCC TTGCCTTTCG 
     1801 ATGGCCAGAA TTTAAAGGAA CTGCGAGAGC GAGTTTTACG AGGGAAGTAC CGTATTCCCT 
     1861 TCTATATGTC CACAGACTGT GAAAATCTTC TGAAGAAATT ATTAGTCCTG AATCCAATAA 
     1921 AGAGAGGCAG CTTGGAACAA ATAATGAAAG ATCGATGGAT GAATGTTGGT CATGAAGAGG 
     1981 AAGAACTAAA GCCATATACT GAGCCTGATC CGGATTTCAA TGACACAAAA AGAATAGACA 
     2041 TTATGGTCAC CATGGGCTTT GCACGAGATG AAATAAATGA TGCCTTAATA AATCAGAAGT 
     2101 ATGATGAAGT TATGGCTACT TATATTCTTC TAGGTAGAAA ACCACCTGAA TTTGAAGGTG 
     2161 GTGAATCGTT ATCCAGTGGA AACTTGTGTC AGAGGTCCCG GCCCAGTAGT GACTTAAACA 
     2221 ACAGCACTCT TCAGTCCCCT GCTCACCTGA AGGTCCAGAG AAGTATCTCA GCAAATCAGA 
     2281 AGCAGCGGCG TTTCAGTGAT CATGCTGGTC CATCCATTCC TCCTGCTGTA TCATATACCA 
     2341 AAAGACCTCA GGCTAACAGT GTGGAAAGTG AACAGAAAGA GGAGTGGGAC AAAGATGTGG 
     2401 CTCGAAAACT TGGCAGCACA ACAGTTGGAT CAAAAAGCGA GATGACTGCA AGCCCTCTTG 
     2461 TAGGGCCAGA GAGGAAAAAA TCTTCAACTA TTCCAAGTAA CAATGTGTAT TCTGGAGGTA 
     2521 GCATGGCAAG AAGGAATACA TATGTCTGTG AAAGGACCAC AGATCGATAC GTAGCATTGC 
     2581 AGAATGGAAA AGACAGCAGC CTTACGGAGA TGTCTGTGAG TAGCATATCT TCTGCAGGCT 
     2641 CTTCTGTGGC CTCTGCTGTC CCCTCAGCAC GACCCCGCCA CCAGAAGTCC ATGTCCACTT 
     2701 CTGGTCATCC TATTAAAGTC ACACTGCCAA CCATTAAAGA CGGCTCTGAA GCTTACCGGC 
     2761 CTGGTACAAC CCAGAGAGTG CCTGCTGCTT CCCCATCTGC TCACAGTATT AGTACTGCGA 
     2821 CTCCAGACCG GACCCGTTTT CCCCGAGGGA GCTCAAGCCG AAGCACTTTC CATGGTGAAC 
     2881 AGCTCCGGGA GCGACGCAGC GTTGCTTATA ATGGGCCACC TGCTTCACCA TCCCATGAAA 
     2941 CGGGTGCATT TGCACATGCC AGAAGGGGAA CGTCAACTGG TATAATAAGC AAAATCACAT 
     3001 CCAAATTTGT TCGCAGGGAT CCAAGTGAAG GCGAAGCCAG TGGCAGAACC GACACCTCAA 
     3061 GAAGTACATC AGGGGAACCA AAAGAAAGAG ACAAGGAAGA GGGTAAAGAT TCTAAGCCGC 
     3121 GTTCTTTGCG GTTCACATGG AGTATGAAGA CCACTAGTTC AATGGACCCT AATGACATGA 
     3181 TGAGAGAAAT CCGAAAAGTG TTAGATGCAA ATAACTGTGA TTATGAGCAA AAAGAGAGAT 
     3241 TTTTGCTTTT CTGTGTCCAT GGAGACGCTA GACAGGATAG CCTCGTGCAG TGGGAGATGG 
     3301 AAGTCTGCAA GTTGCCACGA CTGTCACTTA ATGGGGTTCG CTTCAAGCGA ATATCTGGGA 
     3361 CATCTATTGC CTTTAAGAAC ATTGCATCAA AAATAGCAAA TGAGCTTAAG CTGACGCGTA 
     3421 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3481 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3541 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3601 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3661 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     3721 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     3781 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     3841 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     3901 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     3961 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4021 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4081 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4141 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4201 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4261 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4321 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4381 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4441 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4501 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4561 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4621 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4681 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     4741 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     4801 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     4861 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     4921 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     4981 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5041 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5101 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5161 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5221 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5281 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5341 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5401 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5461 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5521 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5581 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5641 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     5701 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     5761 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     5821 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     5881 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     5941 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6001 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6061 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6121 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6181 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6241 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6301 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6361 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6421 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6481 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6541 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6601 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6661 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     6721 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     6781 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     6841 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     6901 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     6961 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7021 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7081 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7141 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7201 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7261 AATTTT
//