LOCUS       RC221537                8793 bp    DNA     circular  SYN 
DEFINITION  DSPP (Myc-DDK-tagged)-Human dentin sialophosphoprotein (DSPP). Note: ORF is codon optimized
ACCESSION   NM_014208
VERSION     NM_014208.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4948..5039 
                     /label="Myc-DDK"  
     misc_feature    5069..5654 
                     /label="PolyA signal"  
     misc_feature    5126..5145 
                     /label="Restriction Enzyme"  
     misc_feature    5930..6602 
                     /label="ColE1"  
     misc_feature    7032..7826 
                     /label="Kan/Neo"  
     misc_feature    7861..8163 
                     /label="SV40 ori"  
     misc_feature    8249..8726 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4923..4930 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4923 
                     /label="DSPP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAATCATT ACTTATTTTT GCATTTGGGC GGTGGCCTGG GCCATCCCAG 
     1081 TGCCGCAGTC TAAACCACTC GAACGGCACG TAGAGAAAAG TATGAACCTC CACCTGTTGG 
     1141 CACGCTCTAA CGTCAGTGTT CAGGACGAGC TGAATGCTTC CGGTACAATT AAAGAGTCCG 
     1201 GGGTCCTCGT CCACGAAGGC GATCGGGGGA GGCAGGAGAA CACCCAAGAT GGGCACAAGG 
     1261 GAGAGGGTAA CGGCTCCAAG TGGGCCGAGG TCGGAGGAAA ATCCTTTTCC ACTTACTCCA 
     1321 CACTGGCTAA CGAGGAGGGC AACATCGAAG GGTGGAATGG AGATACCGGC AAGGCAGAAA 
     1381 CATATGGACA CGATGGTATA CATGGCAAGG AGGAGAACAT CACTGCCAAT GGCATCCAAG 
     1441 GGCAGGTTTC TATCATTGAC AATGCCGGGG CCACCAATCG GTCTAATACC AACGGGAATA 
     1501 CAGATAAGAA TACCCAGAAT GGGGACGTGG GCGACGCCGG ACACAACGAA GATGTGGCTG 
     1561 TGGTCCAAGA GGATGGCCCC CAGGTGGCAG GATCAAATAA CTCAACTGAC AATGAAGACG 
     1621 AGATCATCGA GAATTCATGC AGGAATGAAG GAAACACAAG CGAGATCACC CCCCAAATTA 
     1681 ACAGTAAGCG GAATGGAACA AAGGAAGCAG AGGTGACCCC AGGCACAGGC GAGGACGCCG 
     1741 GCTTGGATAA CAGTGATGGT TCCCCATCTG GCAATGGCGC CGACGAGGAT GAGGATGAAG 
     1801 GGAGCGGTGA CGATGAGGAC GAAGAAGCAG GAAATGGCAA GGATTCCAGC AACAACTCCA 
     1861 AGGGGCAAGA AGGACAGGAC CATGGGAAGG AAGATGACCA CGATTCTAGT ATAGGTCAAA 
     1921 ATTCCGACTC TAAGGAGTAC TATGACCCCG AGGGAAAAGA GGACCCCCAC AATGAGGTGG 
     1981 ACGGCGACAA GACGAGTAAA AGCGAGGAAA ATTCCGCTGG CATTCCTGAA GACAATGGCA 
     2041 GCCAACGAAT CGAAGATACT CAGAAACTTA ACCACCGGGA GTCCAAGAGA GTGGAGAACC 
     2101 GCATCACGAA GGAATCAGAG ACACACGCCG TGGGCAAGTC CCAGGATAAA GGAATTGAAA 
     2161 TCAAGGGGCC TTCCAGCGGG AACCGCAACA TTACAAAAGA GGTGGGGAAG GGTAACGAAG 
     2221 GCAAGGAAGA TAAAGGGCAG CACGGAATGA TTCTGGGAAA GGGAAACGTG AAAACTCAGG 
     2281 GAGAGGTGGT GAACATCGAG GGTCCCGGCC AAAAGTCTGA ACCCGGAAAT AAAGTAGGTC 
     2341 ACTCTAATAC AGGTAGCGAT AGCAACTCTG ATGGCTATGA CTCTTATGAT TTTGACGATA 
     2401 AGTCAATGCA GGGAGATGAC CCGAACAGTA GCGACGAATC TAATGGTAAC GATGACGCTA 
     2461 ACAGCGAGAG CGATAACAAC AGCTCTTCTC GGGGGGATGC ATCCTATAAT AGCGACGAGA 
     2521 GTAAGGACAA CGGAAATGGA TCCGATTCCA AAGGTGCAGA GGACGACGAC AGTGACAGTA 
     2581 CGTCAGATAC AAACAACTCA GACTCCAATG GAAATGGTAA TAATGGTAAC GATGACAACG 
     2641 ACAAGTCAGA TTCAGGCAAG GGAAAATCCG ACAGCAGCGA TTCTGATAGC TCCGATAGCT 
     2701 CTAACTCAAG TGATTCCTCT GACAGCTCTG ACTCTGATTC TAGTGATAGT AACAGCAGCA 
     2761 GCGATTCCGA CTCCTCTGAC TCTGATAGCA GTGACTCCAG CGATAGCGAT TCTTCCGATA 
     2821 GCTCCAACTC TTCAGATTCT TCAGATAGCA GTGACAGCAG CGACTCATCC GATAGCTCTG 
     2881 ACTCCTCAGA TAGCAAATCA GATTCCTCCA AAAGCGAGTC CGACTCCTCC GACTCCGATT 
     2941 CAAAAAGCGA TAGCAGCGAT TCAAACTCAT CTGACTCCAG TGATAATAGT GATTCCAGTG 
     3001 ATTCTAGCAA TTCTAGCAAC TCCTCTGACT CCAGCGACTC TAGCGACAGT AGCGACTCCT 
     3061 CTTCTTCCTC AGACAGCTCC AATTCTTCAG ATAGCAGCGA CAGCAGCGAT TCCTCTAACT 
     3121 CCTCTGAGAG TAGCGACTCC AGCGATTCAT CCGACAGCGA CAGCAGCGAC TCTTCAGACT 
     3181 CAAGCAACAG TAACTCTTCA GACTCTGACT CTTCCAATTC ATCAGATAGC TCCGACTCAT 
     3241 CAAACTCCTC TGATAGCAGC GACAGCTCAG ACAGCAGTAA CAGCTCTGAC TCTTCCGATT 
     3301 CAAGCGATTC CTCCAACTCC TCAGATAGTT CAGACTCCAG TGACAGCTCC GACAGCAGCG 
     3361 ACTCCAGCAA TTCCTCCGAT TCTAATGATT CCTCTAATTC CTCCGACTCA AGCGATTCAT 
     3421 CTAACAGTAG TGATTCCAGC AACTCAAGTG ATTCCAGCGA CAGCTCCGAT AGCTCAGACT 
     3481 CCGACTCTTC TAATAGTTCC GACAGTAGTA ATTCTAGCGA TAGCTCAGAC TCAAGTAATA 
     3541 GTAGTGACAG CAGTGACTCT AGCGACAGCT CCGATGGTTC TGATAGCGAC TCCAGCAACA 
     3601 GGTCAGATTC ATCTAATTCA TCCGACAGTA GCGACTCATC TGACTCATCT AATAGCTCCG 
     3661 ATTCCTCCGA CTCTAGCGAC TCCAACGAGT CATCTAATAG TTCCGATAGC AGCGACTCAA 
     3721 GCAATTCTAG CGACTCTGAC TCATCCGACA GTAGCAATTC CAGTGACTCC TCTGACAGTT 
     3781 CAAATTCCTC TGATTCCAGT GAATCTAGCA ATTCCAGTGA CAACTCCAAT TCATCTGATT 
     3841 CCTCCAACTC TAGCGATTCT TCCGATTCTT CCGACTCCAG TAATTCATCC GATTCCAGCA 
     3901 ATAGTAGCGA CTCTTCCAAC TCCTCCGACT CATCAGATAG TAACAGTTCC GATTCAAGCG 
     3961 ATAGCAGCAA TTCCAGCGAC TCTTCAGATA GCTCCGACAG CTCCGATTCC TCTGACAGTA 
     4021 GCGATAGTAG CAATAGTAGT GACTCCTCCG ATTCTTCAGA TTCTTCCGAC AGCTCTAATA 
     4081 GCTCCGACTC CAGTAACAGC TCCGACAGCA GCAACAGCAG TGACTCATCC GATAGCTCCG 
     4141 ACTCCTCAGA CAGCAGCGAC TCTAGCGACT CCTCAGACTC CTCTGACTCC AGTAACTCCT 
     4201 CAGATTCCAG TGACAGCAGC GATTCCTCCG ACAGTTCCGA CTCCTCCGAC TCTAGCGATA 
     4261 GTAGTGACAG TTCCGAGTCC TCTGATAGCT CCGACAGTAG TAACAGCAGT GACTCTTCCG 
     4321 ATTCATCAGA CTCATCTGAT TCAAGCGATT CATCAGACTC CTCCGACTCT AGCGATTCAA 
     4381 GTGACTCTAG CAATAGCTCA GACAGCAGCG ATTCAAGTGA TTCAAGCGAC AGTTCTGACA 
     4441 GCAGCAACTC CAGCGACTCC AGCGACTCAT CTGAAAGTAG CGATTCAAGT GACTCATCCG 
     4501 ACTCCTCTGA TAGCAGTGAC AGTTCCGACT CATCTGATTC CAGCGACAGC AGCGATTCCT 
     4561 CTAATTCAAG CGACAGCTCT GATTCAAGCG ACAGCTCCGA CAGCTCCGAT TCCTCAGATA 
     4621 GTAGCGATAG TAGTGATTCC AGCGACTCCA GCGATAGTAG CGATAGCAGT GATTCATCAG 
     4681 ATTCAAGCGA TTCCTCTGAT TCTTCTGACA GTAGTGATAG TAATGAGAGC AGTGACAGTA 
     4741 GTGATAGTAG TGATAGTAGC GATAGTTCAA ATTCCAGCGA CTCTAGTGAT TCCTCTGATT 
     4801 CTTCTGACAG TACCTCTGAC AGCAACGACG AGAGCGATAG TCAGTCTAAG AGTGGCAATG 
     4861 GGAACAATAA TGGCTCCGAT TCAGACTCAG ATAGTGAAGG GTCTGATAGC AACCATTCTA 
     4921 CATCTGATGA TAGCGGACCG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4981 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5041 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5101 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5161 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5221 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5281 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5341 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5401 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5461 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5521 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5581 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5641 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5701 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5761 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5821 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5881 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5941 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6001 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6061 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6121 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6181 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6241 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6301 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6361 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6421 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6481 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6541 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6601 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6661 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6721 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6781 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6841 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6901 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6961 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7021 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7081 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7141 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7201 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7261 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7321 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7381 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7441 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7501 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7561 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7621 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7681 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7741 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7801 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7861 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7921 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7981 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8041 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8101 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8161 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8221 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8281 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8341 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8401 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8461 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8521 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8581 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8641 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8701 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8761 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//