LOCUS       RC221536                8277 bp    DNA     circular  SYN 
DEFINITION  BAG6 (Myc-DDK-tagged)-Human BCL2-associated athanogene 6 (BAG6), transcript variant 1
ACCESSION   NM_004639
VERSION     NM_004639.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4434..4525 
                     /label="Myc-DDK"  
     misc_feature    4555..5140 
                     /label="PolyA signal"  
     misc_feature    4612..4631 
                     /label="Restriction Enzyme"  
     misc_feature    5416..6088 
                     /label="ColE1"  
     misc_feature    6518..7312 
                     /label="Kan/Neo"  
     misc_feature    7347..7649 
                     /label="SV40 ori"  
     misc_feature    7735..8212 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4416..4422 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4416 
                     /label="BAG6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCTAAT GATAGTACCA GTACCGCTGT GGAGGAGCCT GACAGCTTGG 
     1081 AGGTGTTGGT GAAGACCTTG GACTCTCAAA CTCGTACCTT TATTGTGGGG GCCCAGATGA 
     1141 ATGTAAAAGA GTTTAAGGAG CACATTGCTG CCTCTGTCAG CATCCCATCT GAAAAACAAC 
     1201 GGCTCATTTA CCAGGGACGA GTTCTGCAAG ATGATAAGAA GCTTCAGGAA TACAATGTTG 
     1261 GGGGAAAGGT TATCCACCTG GTGGAACGGG CTCCTCCTCA GACTCACCTC CCTTCTGGGG 
     1321 CATCTTCTGG GACGGGGTCT GCCTCAGCCA CTCATGGTGG GGGATCCCCC CCTGGTACTC 
     1381 GGGGGCCTGG GGCCTCTGTT CATGACCGGA ATGCCAACAG CTATGTCATG GTTGGAACCT 
     1441 TCAATCTTCC TAGTGACGGC TCTGCTGTGG ATGTTCACAT CAACATGGAA CAGGCCCCGA 
     1501 TTCAGAGTGA GCCCCGGGTA CGGCTGGTGA TGGCTCAGCA CATGATCAGG GATATACAGA 
     1561 CCTTACTATC CCGGATGGAG ACTCTCCCCT ACCTTCAGTG TCGAGGAGGG CCCCAACCGC 
     1621 AGCACAGTCA GCCGCCCCCG CAGCCACCGG CTGTGACCCC GGAGCCAGTA GCCTTGAGCT 
     1681 CTCAAACATC AGAACCAGTT GAAAGTGAAG CACCTCCCCG GGAGCCCATG GAGGCAGAAG 
     1741 AAGTGGAGGA GCGTGCCCCA GCCCAGAACC CGGAGCTCAC TCCTGGCCCA GCCCCAGCGG 
     1801 GCCCAACACC TGCCCCGGAA ACAAATGCAC CCAACCATCC TTCCCCTGCG GAGTATGTCG 
     1861 AGGTGCTCCA GGAGCTACAG CGGCTGGAGA GTCGCCTCCA GCCCTTCTTG CAGCGCTACT 
     1921 ACGAGGTTCT GGGTGCTGCT GCCACCACGG ACTACAATAA CAATCACGAG GGCCGGGAGG 
     1981 AGGATCAGCG GTTGATCAAC TTGGTAGGGG AGAGCCTGCG ACTGCTGGGC AACACCTTTG 
     2041 TTGCACTGTC TGACCTGCGC TGCAATCTGG CCTGCACGCC CCCACGACAC CTGCATGTGG 
     2101 TCCGGCCTAT GTCTCACTAC ACCACCCCCA TGGTGCTCCA GCAGGCAGCC ATTCCCATAC 
     2161 AGATCAATGT GGGAACCACT GTGACCATGA CAGGAAATGG GACTCGGCCC CCCCCAACTC 
     2221 CCAATGCAGA GGCACCTCCC CCTGGTCCTG GGCAGGCCTC ATCCGTGGCT CCGTCTTCTA 
     2281 CCAATGTCGA GTCCTCAGCT GAGGGGGCTC CCCCGCCAGG TCCAGCTCCC CCGCCAGCCA 
     2341 CCAGCCACCC GAGGGTCATC CGGATTTCCC ACCAGAGTGT GGAACCCGTG GTCATGATGC 
     2401 ACATGAACAT TCAAGATTCT GGCACACAGC CTGGTGGTGT TCCGAGTGCT CCCACTGGCC 
     2461 CCCTGGGACC CCCTGGTCAT GGCCAAACCC TGGGACAGCA GGTGCCAGGC TTCCCAACAG 
     2521 CTCCAACCCG GGTGGTGATT GCCCGGCCCA CTCCTCCACA GGCTCGGCCT TCCCATCCTG 
     2581 GAGGGCCCCC AGTCTCTGGG ACACTGCAGG GCGCCGGTCT GGGTACCAAT GCCTCGTTGG 
     2641 CCCAGATGGT GAGCGGCCTT GTGGGGCAGC TTCTTATGCA GCCAGTCCTT GTGGCTCAGG 
     2701 GGACCCCAGG TATGGCTCCA CCGCCAGCCC CTGCCACTGC TTCTGCCAGT GCTGGCACCA 
     2761 CCAACACAGC TACCACAGCT GGCCCCGCTC CTGGGGGGCC TGCCCAGCCT CCACCCACCC 
     2821 CTCAACCCTC CATGGCTGAT CTTCAGTTCT CTCAGCTTCT GGGGAACCTG CTAGGGCCTG 
     2881 CAGGGCCAGG GGCTGGAGGG TCTGGTGTGG CTTCTCCCAC CATCACTGTG GCGATGCCTG 
     2941 GTGTCCCTGC CTTTCTCCAA GGCATGACTG ACTTCTTGCA GGCAACACAG ACAGCCCCTC 
     3001 CACCACCCCC ACCTCCTCCA CCCCCACCAC CTGCCCCAGA GCAGCAGACC ATGCCCCCAC 
     3061 CAGGCTCCCC TTCTGGTGGC GCAGGGAGTC CTGGAGGCCT GGGTCTTGAG AGCCTGTCAC 
     3121 CGGAGTTTTT TACCTCAGTG GTGCAGGGTG TGCTCAGCTC CCTGCTGGGC TCCCTGGGGG 
     3181 CTCGGGCTGG CAGCAGTGAA AGTATTGCTG CCTTCATACA ACGCCTCAGT GGATCCAGCA 
     3241 ACATCTTTGA GCCTGGAGCT GATGGGGCCC TTGGATTCTT TGGGGCCTTG CTTTCTCTTC 
     3301 TGTGCCAGAA CTTCTCTATG GTGGACGTAG TGATGCTTCT CCATGGGCAT TTCCAGCCAC 
     3361 TACAACGGCT CCAGCCCCAG CTGCGATCCT TCTTCCACCA GCACTACCTG GGTGGTCAGG 
     3421 AGCCCACACC CAGTAACATC CGGATGGCAA CCCACACATT GATCACGGGG CTAGAAGAGT 
     3481 ATGTGCGGGA GAGTTTTTCC TTGGTGCAGG TTCAGCCAGG TGTGGACATC ATCCGGACAA 
     3541 ACCTGGAATT TCTCCAAGAG CAGTTTAATA GCATTGCTGC GCATGTGCTG CATTGCACAG 
     3601 ATAGTGGATT TGGGGCCCGG TTGCTGGAGT TGTGTAACCA AGGCCTGTTT GAATGCCTGG 
     3661 CCCTAAACCT GCACTGCTTG GGGGGACAGC AGATGGAGCT TGCTGCTGTT ATCAATGGCC 
     3721 GAATTCGTCG TATGTCTCGT GGGGTGAATC CCTCCTTGGT GAGCTGGCTG ACCACTATGA 
     3781 TGGGACTGAG GCTTCAGGTG GTACTGGAGC ACATGCCTGT AGGCCCTGAT GCCATTCTCA 
     3841 GATACGTTCG CAGGGTTGGT GATCCCCCCC AGCCACTTCC TGAGGAGCCA ATGGAAGTTC 
     3901 AGGGAGCAGA AAGAGCTTCC CCTGAGCCTC AGCGGGAGAA TGCTTCCCCA GCCCCTGGAA 
     3961 CAACAGCAGA AGAGGCCATG TCCCGAGGTC CACCTCCTGC TCCTGAGGGG GGCTCCCGGG 
     4021 ATGAACAGGA TGGAGCTTCA GCTGAGACAG AACCTTGGGC AGCTGCAGTC CCCCCAGAAT 
     4081 GGGTCCCTAT TATCCAGCAG GACATTCAGA GCCAGCGGAA GGTGAAACCG CAGCCCCCTC 
     4141 TGAGTGATGC CTACCTCAGT GGTATGCCTG CCAAGAGACG CAAGACGATG CAGGGTGAGG 
     4201 GCCCCCAGCT GCTTCTCTCA GAGGCTGTGA GCCGGGCAGC TAAGGCAGCC GGAGCTCGGC 
     4261 CCCTGACGAG CCCCGAGAGC CTGAGCCGGG ACCTGGAGGC ACCAGAGGTT CAGGAGAGCT 
     4321 ACAGGCAGCA GCTCCGGTCT GATATACAAA AACGACTGCA GGAAGACCCC AACTACAGTC 
     4381 CCCAGCGCTT CCCCAATGCC CAGCGGGCCT TTGCTGATGA TCCTACGCGT ACGCGGCCGC 
     4441 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4501 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4561 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4621 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4681 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4741 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4801 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4861 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4921 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4981 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5041 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5101 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5161 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5221 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5281 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5341 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5401 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5461 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5521 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5581 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5641 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5701 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5761 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5821 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5881 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5941 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6001 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6061 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6121 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6181 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6241 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6301 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6361 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6421 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6481 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6541 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6601 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6661 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6721 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6781 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6841 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6901 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6961 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7021 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7081 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7141 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7201 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7261 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7321 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7381 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7441 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7501 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7621 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7681 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7741 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7801 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7861 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7921 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7981 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8041 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8101 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8161 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8221 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//