LOCUS       RC221532                8361 bp    DNA     circular  SYN 
DEFINITION  PRDM10 (Myc-DDK-tagged)-Human PR domain containing 10 (PRDM10), transcript variant 1
ACCESSION   NM_020228
VERSION     NM_020228.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4518..4609 
                     /label="Myc-DDK"  
     misc_feature    4639..5224 
                     /label="PolyA signal"  
     misc_feature    4696..4715 
                     /label="Restriction Enzyme"  
     misc_feature    5500..6172 
                     /label="ColE1"  
     misc_feature    6602..7396 
                     /label="Kan/Neo"  
     misc_feature    7431..7733 
                     /label="SV40 ori"  
     misc_feature    7819..8296 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4500..4506 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4500 
                     /label="PRDM10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATTCCAAA GATGAAAGCT CGCATGTGTG GCCGACATCT GCAGAGCATG 
     1081 AACAGAATGC CGCACAGGTG CACTTTGTTC CGGACACAGG AACAGTGGCT CAGATTGTCT 
     1141 ATACCGATGA CCAGGTTCGC CCCCCACAGC AGGTGGTGTA CACGGCAGAT GGTGCCTCCT 
     1201 ACACATCAGT GGATGGTCCA GAGCACACGC TGGTGTACAT CCACCCGGTG GAAGCTGCAC 
     1261 AGACTCTGTT TACAGACCCC GGCCAGGTAG CTTATGTCCA ACAGGATGCT ACAGCTCAGC 
     1321 AGGCCTCATT GCCAGTTCAT AACCAGGTGC TGCCTTCCAT CGAGAGTGTA GATGGGTCCG 
     1381 ACCCTTTGGC AACTCTGCAG ACCCCTCTAG GCAGACTGGA GGCCAAAGAG GAAGAGGATG 
     1441 AGGATGAGGA CGAGGACACT GAGGAAGATG AGGAAGAAGA CGGTGAGGAC ACGGATCTGG 
     1501 ATGACTGGGA GCCAGACCCG CCCCGGCCCT TCGACCCACA CGACTTGTGG TGTGAGGAGT 
     1561 GCAATAACGC GCATGCTTCA GTGTGTCCGA AGCACGGCCC CTTGCACCCG ATCCCCAACC 
     1621 GGCCGGTGCT CACCCGGGCC AGGGCGAGCC TCCCCCTGGT GCTCTACATA GACAGGTTTC 
     1681 TGGGCGGGGT GTTCTCCAAG CGGCGCATCC CCAAGCGCAC CCAGTTTGGC CCCGTGGAGG 
     1741 GGCCTCTCGT CAGGGGCTCG GAGCTGAAAG ACTGTTACAT TCACCTCAAG GTTTCTCTTG 
     1801 ATAAAGGGGA CAGGAAAGAA AGGGATTTAC ATGAAGACCT ATGGTTTGAG TTGTCTGATG 
     1861 AGACGCTTTG TAACTGGATG ATGTTTGTAC GGCCAGCCCA GAATCACCTG GAGCAGAACC 
     1921 TGGTGGCTTA CCAGTATGGC CACCATGTGT ATTATACAAC CATAAAAAAT GTGGAGCCCA 
     1981 AGCAGGAACT GAAGGTGTGG TATGCCGCAT CCTATGCTGA GTTCGTGAAC CAGAAAATTC 
     2041 ATGACATTTC TGAGGAAGAA AGGAAAGTTC TTCGAGAGCA AGAGAAGAAT TGGCCCTGCT 
     2101 ATGAATGTAA CCGCCGATTT ATAAGCTCGG AGCAGTTGCA ACAGCATCTC AATTCTCATG 
     2161 ATGAGAAACT AGATGTGTTT AGCAGAACAA GAGGCAGAGG AAGGGGACGA GGCAAGAGGC 
     2221 GATTCGGTCC AGGTCGACGG CCGGGGCGTC CTCCAAAATT TATCCGCCTG GAAATCACCA 
     2281 GCGAAAATGG GGAAAAGAGT GACGATGGGA CACAGGACTT GCTACATTTT CCCACAAAGG 
     2341 AGCAATTTGA TGAGGCTGAA CCAGCCACTC TGAATGGGCT GGATCAACCA GAACAGACCA 
     2401 CTATCCCAAT CCCTCAGCTG CCACAGGAAA CCCAGTCTTC CCTGGAACAT GAACCAGAAA 
     2461 CTCACACCCT GCACCTGCAG CCGCAGCATG AAGAGAGCGT GGTGCCCACC CAGAGCACGC 
     2521 TGACAGCCGA CGACATGCGC AGAGCCAAGC GCATCCGATT GGAGCTGCAG AATGCAGCTC 
     2581 TTCAGCATCT GTTTATTCGG AAGTCCTTCC GGCCTTTTAA ATGCTTGCAG TGTGGGAAGG 
     2641 CCTTCCGGGA AAAGGACAAA CTGGACCAGC ACTTACGCTT CCATGGGCGG GAGGGGAACT 
     2701 GCCCACTGAC CTGTGATCTC TGTAACAAGG GCTTCATCAG CAGCACATCC TTGGAGAGCC 
     2761 ACATGAAGCT CCACTCAGAC CAGAAGACTT ACTCTTGCAT TTTTTGCCCA GAATCCTTTG 
     2821 ACCGCCTTGA TTTGTTGAAA GATCATGTGG CCATTCATAT CAATGATGGC TACTTCACCT 
     2881 GCCCAACTTG TAAGAAACGG TTCCCAGATT TTATCCAGGT GAAAAAACAC GTGCGCAGCT 
     2941 TCCACTCAGA AAAGATCTAC CAGTGCACAG AGTGTGACAA GGCCTTCTGT CGCCCCGATA 
     3001 AACTGCGACT CCACATGCTC CGGCATTCGG ACCGCAAAGA CTTCCTGTGT TCCACCTGTG 
     3061 GGAAGCAATT TAAGCGAAAA GACAAACTAC GGGAACACAT GCAGAGGATG CATAATCCTG 
     3121 AGAGGGAGGC CAAGAAAGCC GACCGCATCA GCCGCTCCAA GACGTTCAAG CCCCGCATCA 
     3181 CGTCCACAGA CTACGACAGC TTCACGTTCA AGTGCCGCCT GTGCATGATG GGCTTCCGGC 
     3241 GGCGCGGCAT GCTGGTAAAT CACTTATCGA AGAGACACCC AGACATGAAG ATAGAAGAGG 
     3301 TGCCAGAGTT AACTCTACCC ATCATAAAAC CCAATCGTGA TTACTTTTGT CAGTATTGCG 
     3361 ATAAGGTTTA TAAAAGTGCC AGCAAGCGCA AAGCCCACAT TCTGAAGAAC CACCCAGGAG 
     3421 CAGAGCTCCC ACCGAGCATT CGGAAGCTCC GACCCGCTGG TCCTGGAGAG CCAGACCCCA 
     3481 TGCTGAGCAC ACACACCCAG CTGACGGGCA CCATCGCCAC CCCTCCCGTC TGCTGTCCCC 
     3541 ACTGCTCCAA GCAGTACAGC AGCAAGACCA AGATGGTCCA GCACATTCGA AAGAAGCATC 
     3601 CAGAGTTCGC CCAGCTCTCC AACACCATAC ACACACCACT GACGACAGCT GTGATCAGTG 
     3661 CCACCCCAGC GGTTTTGACT ACAGACAGCG CCACTGGAGA GACTGTGGTG ACGACGGACC 
     3721 TGCTCACCCA AGCAATGACA GAACTGTCCC AGACCTTAAC GACAGACTAC CGAACGCCAC 
     3781 AAGGGGATTA CCAGAGAATT CAGTACATCC CTGTGTCGCA GTCGGCGTCT GGCCTCCAGC 
     3841 AGCCTCAGCA CATACAGCTG CAAGTGGTTC AAGTGGCCTC GGCCACTTCC CCTCACCAGT 
     3901 CACAGCAGTC CACTGTGGAT GTTGGCCAGC TCCATGATCC TCAGCCCTAC CCCCAGCACG 
     3961 CCATCCAGGT GCAGCACATC CAGGTCAGCG AGCCTACCGC CTCGGCCCCG TCCTCCGCCC 
     4021 AGGTATCTGG GCAGCCGTTG AGTCCCTCAG CCCAGCAGGC TCAGCAGGGG CTCAGCCCCT 
     4081 CCCACATCCA GGGCAGTTCT TCCACACAGG GGCAGGCTCT GCAGCAGCAG CAGCAGCAGC 
     4141 AGCAGAATTC CTCTGTGCAG CACACGTACC TGCCCAGTGC TTGGAATTCC TTCCGTGGCT 
     4201 ATTCATCTGA GATTCAAATG ATGACGCTTC CTCCGGGTCA GTTTGTGATT ACAGACAGTG 
     4261 GTGTGGCAAC TCCAGTTACT ACTGGCCAGG TGAAGGCGGT TACTTCGGGT CATTATGTGT 
     4321 TATCAGAAAG TCAATCAGAA TTGGAAGAAA AGCAAACTTC TGCCCTCTCT GGTGGAGTCC 
     4381 AGGTCGAGCC ACCTGCACAC AGTGACTCCC TGGACCCCCA GACCAACAGC CAACAGCAGA 
     4441 CCACACAGTA CATCATCACC ACCACCACCA ACGGGAACGG AAGCAGCGAA GTGCATATCA 
     4501 CCAAACCAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4561 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4621 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4681 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4741 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4801 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4861 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4921 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4981 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5041 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5101 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5161 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5221 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5281 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5341 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5401 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5461 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5521 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5581 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5641 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5701 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5761 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5821 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5881 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5941 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6001 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6061 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6121 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6181 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6241 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6301 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6361 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6421 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6481 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6541 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6601 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6661 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6721 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6781 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6841 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6901 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6961 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7021 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7081 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7141 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7201 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7261 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7321 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7381 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7441 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7501 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7561 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7801 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7861 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7921 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7981 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8041 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8101 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8161 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8221 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8281 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8341 CAAAAATTTA ACGCGAATTT T
//