LOCUS       RC221451L1                9099 bp    DNA     circular  SYN 
DEFINITION  GEN1 (Myc-DDK-tagged)-Human Gen homolog 1, endonuclease (GEN1), transcript variant 1
ACCESSION   NM_182625
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7390..7570 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8298..8957 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7729..8297 
                     /label="ColE1"  
     misc_feature    6265..6285 
                     /label="LR50 primer"  
     misc_feature    6062..6133 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6274..6865 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    6044..6050 
                     /label="Restriction Enzyme"  
     misc_feature    3320..6044 
                     /label="GEN1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGAGTGAAT GACTTGTGGC AAATTTTGGA 
     3361 GCCTGTTAAG CAACACATCC CCTTGCGTAA TCTTGGTGGG AAAACCATTG CAGTTGATCT 
     3421 GAGTCTCTGG GTGTGTGAGG CACAGACAGT CAAAAAAATG ATGGGCAGCG TCATGAAGCC 
     3481 CCACCTCAGG AACTTATTTT TTCGTATCTC ATATTTAACA CAAATGGATG TAAAACTGGT 
     3541 ATTTGTTATG GAAGGGGAAC CACCAAAGCT GAAAGCTGAT GTCATAAGCA AGAGGAATCA 
     3601 GACTCGGTAT GGGTCTTCTG GAAAATCGTG GTCTCAGAAA ACAGGGAGAT CACATTTTAA 
     3661 ATCAGTCTTA AGAGAGTGCC TCCATATGCT CGAATGCTTA GGAATCCCCT GGGTTCAGGC 
     3721 TGCTGGGGAA GCTGAAGCCA TGTGTGCTTA TCTCAATGCT GGTGGTCATG TCGATGGCTG 
     3781 CCTCACCAAT GATGGAGATA CTTTCCTTTA TGGGGCCCAG ACTGTTTACA GGAATTTCAC 
     3841 TATGAATACA AAGGACCCAC ATGTTGACTG TTACACAATG TCATCTATCA AGAGTAAACT 
     3901 AGGTTTGGAT AGAGATGCTC TGGTTGGATT AGCAATACTT CTTGGCTGTG ATTATCTCCC 
     3961 AAAGGGAGTC CCTGGAGTTG GAAAAGAGCA AGCATTAAAA CTTATACAGA TTTTGAAAGG 
     4021 GCAAAGTTTA CTTCAGAGGT TTAATCGGTG GAATGAAACA TCTTGTAACT CTAGTCCACA 
     4081 ACTGCTAGTC ACTAAAAAAC TGGCTCATTG TTCCGTATGT TCCCATCCAG GTTCACCTAA 
     4141 GGATCATGAA CGTAATGGAT GCAGATTATG TAAAAGTGAT AAATATTGTG AGCCACATGA 
     4201 CTATGAATAC TGCTGTCCTT GTGAGTGGCA CCGTACAGAA CATGATAGGC AACTCAATGA 
     4261 AGTAGAGAAC AATATTAAGA AGAAAGCTTG CTGTTGTGAG GGATTCCCAT TCCATGAGGT 
     4321 TATTCAAGAA TTCCTTTTAA ACAAGGATAA ATTGGTGAAG GTTATCAGGT ACCAAAGACC 
     4381 TGATTTGTTA TTGTTTCAGA GATTTACTCT TGAAAAAATG GAGTGGCCCA ATCACTATGC 
     4441 ATGTGAGAAA TTGCTGGTAC TTTTGACCCA TTATGACATG ATAGAAAGAA AGCTTGGTAG 
     4501 CAGAAACTCT AATCAACTAC AGCCAATTCG AATTGTTAAG ACTCGAATCA GAAATGGAGT 
     4561 TCATTGTTTT GAAATAGAAT GGGAAAAGCC TGAACATTAT GCTATGGAAG ATAAACAACA 
     4621 TGGAGAATTT GCTTTATTAA CAATTGAGGA AGAATCATTG TTTGAAGCAG CATATCCTGA 
     4681 GATCGTTGCT GTTTACCAAA AACAAAAGTT AGAAATTAAA GGGAAGAAAC AAAAACGTAT 
     4741 TAAGCCTAAA GAAAACAATT TGCCAGAACC AGATGAAGTA ATGAGCTTTC AGTCACACAT 
     4801 GACTTTAAAA CCCACATGTG AAATCTTTCA TAAGCAGAAT TCCAAGTTAA ATTCGGGGAT 
     4861 TTCCCCTGAT CCTACATTAC CACAGGAATC TATTTCTGCC TCATTGAATA GCTTGCTTTT 
     4921 ACCTAAAAAT ACTCCATGTT TGAATGCACA AGAACAGTTC ATGTCTTCTC TAAGACCTTT 
     4981 GGCTATACAG CAAATTAAAG CTGTCAGTAA GTCTCTAATT TCAGAATCTA GTCAACCCAA 
     5041 TACCTCATCT CATAATATAT CCGTGATTGC TGATCTACAC TTGAGCACTA TTGACTGGGA 
     5101 AGGTACTTCT TTTAGTAATT CTCCAGCTAT TCAAAGGAAT ACTTTTTCTC ATGATTTAAA 
     5161 ATCAGAAGTT GAATCAGAGC TATCAGCCAT CCCTGATGGC TTTGAAAATA TCCCAGAACA 
     5221 ACTGTCCTGT GAATCAGAAA GGTACACTGC AAACATAAAG AAAGTGTTGG ATGAGGATTC 
     5281 TGATGGGATT AGTCCTGAAG AGCATCTACT TTCTGGCATT ACTGATTTAT GTCTTCAGGA 
     5341 TTTGCCTTTA AAGGAACGAA TATTTACAAA ATTATCATAT CCTCAGGATA ATCTACAACC 
     5401 AGATGTCAAC CTGAAAACTT TGTCCATACT TAGTGTAAAA GAATCTTGTA TTGCTAACAG 
     5461 TGGTTCTGAT TGTACATCAC ATCTTTCAAA GGATCTTCCA GGAATTCCCT TGCAAAATGA 
     5521 ATCCAGAGAC TCTAAAATTC TAAAAGGAGA CCAGCTGCTT CAAGAAGACT ATAAAGTCAA 
     5581 TACTTCTGTC CCTTATTCTG TCAGTAACAC AGTGGTAAAG ACCTGCAATG TTAGACCACC 
     5641 AAATACTGCT TTAGATCATA GTAGAAAAGT TGATATGCAA ACCACTCGGA AAATTTTAAT 
     5701 GAAGAAGAGT GTTTGCCTTG ACAGACATTC CTCTGATGAA CAAAGTGCCC CAGTGTTTGG 
     5761 GAAAGCTAAG TACACAACTC AAAGAATGAA GCACAGTTCT CAAAAGCATA ATTCATCCCA 
     5821 TTTCAAAGAA AGTGGCCATA ACAAGTTGAG TAGCCCTAAG ATACATATTA AAGAAACTGA 
     5881 ACAGTGTGTC AGATCTTATG AAACAGCTGA AAATGAAGAA AGCTGTTTCC CAGATTCAAC 
     5941 AAAAAGTTCT CTGAGTTCTC TACAATGTCA TAAGAAAGAA AACAACTCTG GTACTTGTTT 
     6001 GGATAGCCCT CTTCCTTTAC GCCAGAGATT AAAACTAAGA TTCCAAAGCA CTACGCGTAC 
     6061 GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA 
     6121 TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCTGTACA AGTAGGATTC 
     6181 GTCGAGGGAC CTAATAACTT CGTATAGCAT ACATTATACG AAGTTATACA TGTTTAAGGG 
     6241 TTCCGGTTCC ACTAGGTACA ATTCGATATC AAGCTTATCG ATAATCAACC TCTGGATTAC 
     6301 AAAATTTGTG AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA 
     6361 TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC 
     6421 TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA 
     6481 CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC 
     6541 ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC 
     6601 ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC 
     6661 GTGGTGTTGT CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG 
     6721 ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT 
     6781 TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG 
     6841 AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CATCGATACC GTCGACCTCG ATCGAGACCT 
     6901 AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG 
     6961 GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTCCAGTC ACACCTCAGG TACCTTTAAG 
     7021 ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT 
     7081 GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATATCCTT GATCTGTGGA TCTACCACAC 
     7141 ACAAGGCTAC TTCCCTGATT GGCAGAACTA CACACCAGGG CCAGGGATCA GATATCCACT 
     7201 GACCTTTGGA TGGTGCTACA AGCTAGTACC AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA 
     7261 TGAAGGAGAG AACACCCGCT TGTTACACCC TGTGAGCCTG CATGGGATGG ATGACCCGGA 
     7321 GAGAGAAGTA TTAGAGTGGA GGTTTGACAG CCGCCTAGCA TTTCATCACA TGGCCCGAGA 
     7381 GCTGCATCCG GACTGTACTG GGTCTCTCTG GTTAGACCAG ATCTGAGCCT GGGAGCTCTC 
     7441 TGGCTAACTA GGGAACCCAC TGCTTAAGCC TCAATAAAGC TTGCCTTGAG TGCTTCAAGT 
     7501 AGTGTGTGCC CGTCTGTTGT GTGACTCTGG TAACTAGAGA TCCCTCAGAC CCTTTTAGTC 
     7561 AGTGTGGAAA ATCTCTAGCA GCATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     7621 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     7681 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     7741 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     7801 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     7861 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     7921 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     7981 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     8041 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     8101 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     8161 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     8221 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     8281 CTCACGTTAA GGGATTTTGG TCATGATTAC GCCCCGCCCT GCCACTCATC GCAGTACTGT 
     8341 TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC AAACGGCATG ATGAACCTGA 
     8401 ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT ATTTGCCCAT GGTGAAAACG 
     8461 GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA AACTGGTGAA ACTCACCCAG 
     8521 GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT TAGGGAAATA GGCCAGGTTT 
     8581 TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG 
     8641 TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT GGAAAACGGT GTAACAAGGG 
     8701 TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG CCATACGGAA CTCCGGATGA 
     8761 GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT AAAACTTGTG CTTATTTTTC 
     8821 TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG TCTGGTTATA GGTACATTGA 
     8881 GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC ATTGGGATAT ATCAACGGTG 
     8941 GTATATCCAG TGATTTTTTT CTCCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     9001 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     9061 GGGGTCCCGC GCACATTTCC CCGAAAAGTG CCACCTGAC
//