LOCUS       RC221451                7605 bp    DNA     circular  SYN 
DEFINITION  GEN1 (Myc-DDK-tagged)-Human Gen homolog 1, endonuclease (Drosophila) (GEN1), transcript variant 1
ACCESSION   NM_182625
VERSION     NM_182625.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3762..3853 
                     /label="Myc-DDK"  
     misc_feature    3883..4468 
                     /label="PolyA signal"  
     misc_feature    3940..3959 
                     /label="Restriction Enzyme"  
     misc_feature    4744..5416 
                     /label="ColE1"  
     misc_feature    5846..6640 
                     /label="Kan/Neo"  
     misc_feature    6675..6977 
                     /label="SV40 ori"  
     misc_feature    7063..7540 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3744..3750 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3744 
                     /label="GEN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGTGAAT GACTTGTGGC AAATTTTGGA GCCTGTTAAG CAACACATCC 
     1081 CCTTGCGTAA TCTTGGTGGG AAAACCATTG CAGTTGATCT GAGTCTCTGG GTGTGTGAGG 
     1141 CACAGACAGT CAAAAAAATG ATGGGCAGCG TCATGAAGCC CCACCTCAGG AACTTATTTT 
     1201 TTCGTATCTC ATATTTAACA CAAATGGATG TAAAACTGGT ATTTGTTATG GAAGGGGAAC 
     1261 CACCAAAGCT GAAAGCTGAT GTCATAAGCA AGAGGAATCA GACTCGGTAT GGGTCTTCTG 
     1321 GAAAATCGTG GTCTCAGAAA ACAGGGAGAT CACATTTTAA ATCAGTCTTA AGAGAGTGCC 
     1381 TCCATATGCT CGAATGCTTA GGAATCCCCT GGGTTCAGGC TGCTGGGGAA GCTGAAGCCA 
     1441 TGTGTGCTTA TCTCAATGCT GGTGGTCATG TCGATGGCTG CCTCACCAAT GATGGAGATA 
     1501 CTTTCCTTTA TGGGGCCCAG ACTGTTTACA GGAATTTCAC TATGAATACA AAGGACCCAC 
     1561 ATGTTGACTG TTACACAATG TCATCTATCA AGAGTAAACT AGGTTTGGAT AGAGATGCTC 
     1621 TGGTTGGATT AGCAATACTT CTTGGCTGTG ATTATCTCCC AAAGGGAGTC CCTGGAGTTG 
     1681 GAAAAGAGCA AGCATTAAAA CTTATACAGA TTTTGAAAGG GCAAAGTTTA CTTCAGAGGT 
     1741 TTAATCGGTG GAATGAAACA TCTTGTAACT CTAGTCCACA ACTGCTAGTC ACTAAAAAAC 
     1801 TGGCTCATTG TTCCGTATGT TCCCATCCAG GTTCACCTAA GGATCATGAA CGTAATGGAT 
     1861 GCAGATTATG TAAAAGTGAT AAATATTGTG AGCCACATGA CTATGAATAC TGCTGTCCTT 
     1921 GTGAGTGGCA CCGTACAGAA CATGATAGGC AACTCAATGA AGTAGAGAAC AATATTAAGA 
     1981 AGAAAGCTTG CTGTTGTGAG GGATTCCCAT TCCATGAGGT TATTCAAGAA TTCCTTTTAA 
     2041 ACAAGGATAA ATTGGTGAAG GTTATCAGGT ACCAAAGACC TGATTTGTTA TTGTTTCAGA 
     2101 GATTTACTCT TGAAAAAATG GAGTGGCCCA ATCACTATGC ATGTGAGAAA TTGCTGGTAC 
     2161 TTTTGACCCA TTATGACATG ATAGAAAGAA AGCTTGGTAG CAGAAACTCT AATCAACTAC 
     2221 AGCCAATTCG AATTGTTAAG ACTCGAATCA GAAATGGAGT TCATTGTTTT GAAATAGAAT 
     2281 GGGAAAAGCC TGAACATTAT GCTATGGAAG ATAAACAACA TGGAGAATTT GCTTTATTAA 
     2341 CAATTGAGGA AGAATCATTG TTTGAAGCAG CATATCCTGA GATCGTTGCT GTTTACCAAA 
     2401 AACAAAAGTT AGAAATTAAA GGGAAGAAAC AAAAACGTAT TAAGCCTAAA GAAAACAATT 
     2461 TGCCAGAACC AGATGAAGTA ATGAGCTTTC AGTCACACAT GACTTTAAAA CCCACATGTG 
     2521 AAATCTTTCA TAAGCAGAAT TCCAAGTTAA ATTCGGGGAT TTCCCCTGAT CCTACATTAC 
     2581 CACAGGAATC TATTTCTGCC TCATTGAATA GCTTGCTTTT ACCTAAAAAT ACTCCATGTT 
     2641 TGAATGCACA AGAACAGTTC ATGTCTTCTC TAAGACCTTT GGCTATACAG CAAATTAAAG 
     2701 CTGTCAGTAA GTCTCTAATT TCAGAATCTA GTCAACCCAA TACCTCATCT CATAATATAT 
     2761 CCGTGATTGC TGATCTACAC TTGAGCACTA TTGACTGGGA AGGTACTTCT TTTAGTAATT 
     2821 CTCCAGCTAT TCAAAGGAAT ACTTTTTCTC ATGATTTAAA ATCAGAAGTT GAATCAGAGC 
     2881 TATCAGCCAT CCCTGATGGC TTTGAAAATA TCCCAGAACA ACTGTCCTGT GAATCAGAAA 
     2941 GGTACACTGC AAACATAAAG AAAGTGTTGG ATGAGGATTC TGATGGGATT AGTCCTGAAG 
     3001 AGCATCTACT TTCTGGCATT ACTGATTTAT GTCTTCAGGA TTTGCCTTTA AAGGAACGAA 
     3061 TATTTACAAA ATTATCATAT CCTCAGGATA ATCTACAACC AGATGTCAAC CTGAAAACTT 
     3121 TGTCCATACT TAGTGTAAAA GAATCTTGTA TTGCTAACAG TGGTTCTGAT TGTACATCAC 
     3181 ATCTTTCAAA GGATCTTCCA GGAATTCCCT TGCAAAATGA ATCCAGAGAC TCTAAAATTC 
     3241 TAAAAGGAGA CCAGCTGCTT CAAGAAGACT ATAAAGTCAA TACTTCTGTC CCTTATTCTG 
     3301 TCAGTAACAC AGTGGTAAAG ACCTGCAATG TTAGACCACC AAATACTGCT TTAGATCATA 
     3361 GTAGAAAAGT TGATATGCAA ACCACTCGGA AAATTTTAAT GAAGAAGAGT GTTTGCCTTG 
     3421 ACAGACATTC CTCTGATGAA CAAAGTGCCC CAGTGTTTGG GAAAGCTAAG TACACAACTC 
     3481 AAAGAATGAA GCACAGTTCT CAAAAGCATA ATTCATCCCA TTTCAAAGAA AGTGGCCATA 
     3541 ACAAGTTGAG TAGCCCTAAG ATACATATTA AAGAAACTGA ACAGTGTGTC AGATCTTATG 
     3601 AAACAGCTGA AAATGAAGAA AGCTGTTTCC CAGATTCAAC AAAAAGTTCT CTGAGTTCTC 
     3661 TACAATGTCA TAAGAAAGAA AACAACTCTG GTACTTGTTT GGATAGCCCT CTTCCTTTAC 
     3721 GCCAGAGATT AAAACTAAGA TTCCAAAGCA CTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3781 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3841 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     3901 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     3961 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4021 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4081 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4141 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4201 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4261 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4321 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4381 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4441 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4501 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4561 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4621 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4681 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4741 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4801 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4861 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     4921 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     4981 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5041 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5101 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5161 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5221 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5281 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5341 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5401 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5461 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5521 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5581 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5641 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5701 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5761 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5821 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     5881 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     5941 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6001 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6061 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6121 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6181 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6241 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6301 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6361 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6421 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6481 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6541 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6601 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6661 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6721 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6781 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6841 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     6901 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     6961 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7021 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7081 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7141 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7201 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7261 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7321 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7381 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7441 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7501 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7561 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//